Dissociation in control of physiological and behavioral responses to emotional stress by cholinergic neurotransmission in the bed nucleus of the stria terminalis in rats

Detalhes bibliográficos
Autor(a) principal: Gouveia, Marianna K. [UNESP]
Data de Publicação: 2016
Outros Autores: Miguel, Tarciso T., Busnardo, Cristiane, Scopinho, Am�rica A., Corr�a, Fernando M.A., Nunes-De-Souza, Ricardo L. [UNESP], Crestani, Carlos C. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.neuropharm.2015.10.018
http://hdl.handle.net/11449/168088
Resumo: The bed nucleus of the stria terminalis (BNST) is a forebrain structure implicated in physiological and behavioral responses to emotional stress. However, the local neurochemical mechanisms mediating the BNST control of stress responses are not fully known. Here, we investigated the involvement of BNST cholinergic neurotransmission, acting via muscarinic receptors, in cardiovascular (increase in blood pressure and heart rate and fall in tail skin temperature) and neuroendocrine (increase in plasma corticosterone) responses and behavioral consequences (anxiogenic-like effect in the elevated plus-maze) evoked by acute restraint stress in rats. Bilateral microinjection into the BNST of either the choline uptake inhibitor hemicholinium-3 (3 nmol/100 nl) or the muscarinic receptor antagonist methylatropine (3 nmol/100 nl) enhanced the heart rate increase and inhibited the anxiogenic-like effect observed in the elevated plus-maze evoked by restraint. However, neither hemicholinium-3 nor methylatropine affected the increase in blood pressure and plasma corticosterone levels and the fall in tail skin temperature. Facilitation of local cholinergic signaling by microinjection of the acetylcholinesterase inhibitor neostigmine (0.1 nmol/100 nl) into the BNST reduced restraint-evoked pressor and tachycardiac responses and the fall in tail cutaneous temperature, without affecting the increase in plasma corticosterone. All effects of neostigmine were completely abolished by local BNST pretreatment with methylatropine. These findings indicate an opposite role of BNST cholinergic neurotransmission, acting via local muscarinic receptor, in control of cardiovascular responses (inhibitory influence) and emotional consequences (facilitatory influence) evoked by restraint stress. Furthermore, present findings provide evidence that BNST control of neuroendocrine responses to stress is mediated by mechanisms others than local cholinergic signaling.
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spelling Dissociation in control of physiological and behavioral responses to emotional stress by cholinergic neurotransmission in the bed nucleus of the stria terminalis in ratsAnxietyBNSTCardiovascularCorticosteroneElevated plus mazeExtended amygdalaMuscarinic receptorsThe bed nucleus of the stria terminalis (BNST) is a forebrain structure implicated in physiological and behavioral responses to emotional stress. However, the local neurochemical mechanisms mediating the BNST control of stress responses are not fully known. Here, we investigated the involvement of BNST cholinergic neurotransmission, acting via muscarinic receptors, in cardiovascular (increase in blood pressure and heart rate and fall in tail skin temperature) and neuroendocrine (increase in plasma corticosterone) responses and behavioral consequences (anxiogenic-like effect in the elevated plus-maze) evoked by acute restraint stress in rats. Bilateral microinjection into the BNST of either the choline uptake inhibitor hemicholinium-3 (3 nmol/100 nl) or the muscarinic receptor antagonist methylatropine (3 nmol/100 nl) enhanced the heart rate increase and inhibited the anxiogenic-like effect observed in the elevated plus-maze evoked by restraint. However, neither hemicholinium-3 nor methylatropine affected the increase in blood pressure and plasma corticosterone levels and the fall in tail skin temperature. Facilitation of local cholinergic signaling by microinjection of the acetylcholinesterase inhibitor neostigmine (0.1 nmol/100 nl) into the BNST reduced restraint-evoked pressor and tachycardiac responses and the fall in tail cutaneous temperature, without affecting the increase in plasma corticosterone. All effects of neostigmine were completely abolished by local BNST pretreatment with methylatropine. These findings indicate an opposite role of BNST cholinergic neurotransmission, acting via local muscarinic receptor, in control of cardiovascular responses (inhibitory influence) and emotional consequences (facilitatory influence) evoked by restraint stress. Furthermore, present findings provide evidence that BNST control of neuroendocrine responses to stress is mediated by mechanisms others than local cholinergic signaling.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Minist�rio da Ci�ncia, Tecnologia e Inova��oLaboratory of Pharmacology School of Pharmaceutical Sciences Univ. Estadual Paulista-UNESPInstitute of Biomedical Sciences Federal University of Uberl�ndia (UFU)Department of Pharmacology School of Medicine of Ribeir�o Preto University of S�o PauloJoint UFSCar-UNESP Graduate Program in Physiological SciencesLaboratory of Pharmacology School of Pharmaceutical Sciences Univ. Estadual Paulista-UNESPJoint UFSCar-UNESP Graduate Program in Physiological SciencesFAPESP: 2012/14376-0CNPq: 2012/50549-6FAPESP: 2012/50549-6Minist�rio da Ci�ncia, Tecnologia e Inova��o: 2012/50549-6FAPESP: 2013/01283-6FAPESP: 2015/05922-9CNPq: 305597/2012-4CNPq: 456405/2014-3CNPq: 478696/2013-2Universidade Estadual Paulista (Unesp)Federal University of Uberl�ndia (UFU)University of S�o PauloGouveia, Marianna K. [UNESP]Miguel, Tarciso T.Busnardo, CristianeScopinho, Am�rica A.Corr�a, Fernando M.A.Nunes-De-Souza, Ricardo L. [UNESP]Crestani, Carlos C. [UNESP]2018-12-11T16:39:42Z2018-12-11T16:39:42Z2016-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article379-388application/pdfhttp://dx.doi.org/10.1016/j.neuropharm.2015.10.018Neuropharmacology, v. 101, p. 379-388.1873-70640028-3908http://hdl.handle.net/11449/16808810.1016/j.neuropharm.2015.10.0182-s2.0-849449301362-s2.0-84944930136.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengNeuropharmacology2,043info:eu-repo/semantics/openAccess2023-12-25T06:26:12Zoai:repositorio.unesp.br:11449/168088Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:17:36.439640Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Dissociation in control of physiological and behavioral responses to emotional stress by cholinergic neurotransmission in the bed nucleus of the stria terminalis in rats
title Dissociation in control of physiological and behavioral responses to emotional stress by cholinergic neurotransmission in the bed nucleus of the stria terminalis in rats
spellingShingle Dissociation in control of physiological and behavioral responses to emotional stress by cholinergic neurotransmission in the bed nucleus of the stria terminalis in rats
Gouveia, Marianna K. [UNESP]
Anxiety
BNST
Cardiovascular
Corticosterone
Elevated plus maze
Extended amygdala
Muscarinic receptors
title_short Dissociation in control of physiological and behavioral responses to emotional stress by cholinergic neurotransmission in the bed nucleus of the stria terminalis in rats
title_full Dissociation in control of physiological and behavioral responses to emotional stress by cholinergic neurotransmission in the bed nucleus of the stria terminalis in rats
title_fullStr Dissociation in control of physiological and behavioral responses to emotional stress by cholinergic neurotransmission in the bed nucleus of the stria terminalis in rats
title_full_unstemmed Dissociation in control of physiological and behavioral responses to emotional stress by cholinergic neurotransmission in the bed nucleus of the stria terminalis in rats
title_sort Dissociation in control of physiological and behavioral responses to emotional stress by cholinergic neurotransmission in the bed nucleus of the stria terminalis in rats
author Gouveia, Marianna K. [UNESP]
author_facet Gouveia, Marianna K. [UNESP]
Miguel, Tarciso T.
Busnardo, Cristiane
Scopinho, Am�rica A.
Corr�a, Fernando M.A.
Nunes-De-Souza, Ricardo L. [UNESP]
Crestani, Carlos C. [UNESP]
author_role author
author2 Miguel, Tarciso T.
Busnardo, Cristiane
Scopinho, Am�rica A.
Corr�a, Fernando M.A.
Nunes-De-Souza, Ricardo L. [UNESP]
Crestani, Carlos C. [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Federal University of Uberl�ndia (UFU)
University of S�o Paulo
dc.contributor.author.fl_str_mv Gouveia, Marianna K. [UNESP]
Miguel, Tarciso T.
Busnardo, Cristiane
Scopinho, Am�rica A.
Corr�a, Fernando M.A.
Nunes-De-Souza, Ricardo L. [UNESP]
Crestani, Carlos C. [UNESP]
dc.subject.por.fl_str_mv Anxiety
BNST
Cardiovascular
Corticosterone
Elevated plus maze
Extended amygdala
Muscarinic receptors
topic Anxiety
BNST
Cardiovascular
Corticosterone
Elevated plus maze
Extended amygdala
Muscarinic receptors
description The bed nucleus of the stria terminalis (BNST) is a forebrain structure implicated in physiological and behavioral responses to emotional stress. However, the local neurochemical mechanisms mediating the BNST control of stress responses are not fully known. Here, we investigated the involvement of BNST cholinergic neurotransmission, acting via muscarinic receptors, in cardiovascular (increase in blood pressure and heart rate and fall in tail skin temperature) and neuroendocrine (increase in plasma corticosterone) responses and behavioral consequences (anxiogenic-like effect in the elevated plus-maze) evoked by acute restraint stress in rats. Bilateral microinjection into the BNST of either the choline uptake inhibitor hemicholinium-3 (3 nmol/100 nl) or the muscarinic receptor antagonist methylatropine (3 nmol/100 nl) enhanced the heart rate increase and inhibited the anxiogenic-like effect observed in the elevated plus-maze evoked by restraint. However, neither hemicholinium-3 nor methylatropine affected the increase in blood pressure and plasma corticosterone levels and the fall in tail skin temperature. Facilitation of local cholinergic signaling by microinjection of the acetylcholinesterase inhibitor neostigmine (0.1 nmol/100 nl) into the BNST reduced restraint-evoked pressor and tachycardiac responses and the fall in tail cutaneous temperature, without affecting the increase in plasma corticosterone. All effects of neostigmine were completely abolished by local BNST pretreatment with methylatropine. These findings indicate an opposite role of BNST cholinergic neurotransmission, acting via local muscarinic receptor, in control of cardiovascular responses (inhibitory influence) and emotional consequences (facilitatory influence) evoked by restraint stress. Furthermore, present findings provide evidence that BNST control of neuroendocrine responses to stress is mediated by mechanisms others than local cholinergic signaling.
publishDate 2016
dc.date.none.fl_str_mv 2016-02-01
2018-12-11T16:39:42Z
2018-12-11T16:39:42Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.neuropharm.2015.10.018
Neuropharmacology, v. 101, p. 379-388.
1873-7064
0028-3908
http://hdl.handle.net/11449/168088
10.1016/j.neuropharm.2015.10.018
2-s2.0-84944930136
2-s2.0-84944930136.pdf
url http://dx.doi.org/10.1016/j.neuropharm.2015.10.018
http://hdl.handle.net/11449/168088
identifier_str_mv Neuropharmacology, v. 101, p. 379-388.
1873-7064
0028-3908
10.1016/j.neuropharm.2015.10.018
2-s2.0-84944930136
2-s2.0-84944930136.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Neuropharmacology
2,043
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 379-388
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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