Introduction of Trichoderma spp. biocontrol strains against Sclerotinia sclerotiorum (Lib.) de Bary change soil microbial community composition in common bean (Phaseolus vulgaris L.) cultivation
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.biocontrol.2021.104755 http://hdl.handle.net/11449/229513 |
Resumo: | Biological control represents a promising alternative to maintain the viability and productivity of different agricultural crops. In this work, dry bean (Phaseolus vulgaris L.) was grown in a field contaminated with Sclerotinia sclerotiorum (Lib.) de Bary, the causal agent of white mold disease. We analyzed the impact of the application of antagonistic strains of Trichoderma harzianum ESALQ-1306 and Trichoderma asperellum BRM-29104 on the edaphic native microbiota and endophytic fungal community. The structure of the fungal and bacterial communities was evaluated by next generation sequencing of the 28S and 16S rRNA genes, respectively. The results showed that the introduction of the strains changed the composition of the community without significantly affecting the parameters of abundance and alpha diversity (Anova P > 0.05). Ascomycota (54.8%) and Actinobacteria (37.2%) were predominant in the analyzed communities. Hill's diversity profiles of the treatments were similar in terms of diversity and dominance, being higher in a 90-day period and in the control treatment. NMDS and UPGMA analysis show that treatments of different lengths have low similarity in their composition, however, treatments where Trichoderma spp. were inoculated were relatively similar to the control. T. harzianum ESALQ-1306 promoted a greater quantity of indicator OTUs, 17 in total. T. harzianum ESALQ-1306 and T. asperellum BRM-29104 alter the size (1140 and 449 nodes respectively), connectivity parameters such as average degree (79.28 and 22.29 links respectively) and predominant topological roles in co-occurrence networks, but they do not alter the richness or abundance of orthologous genes. Cultivation time and plant organs were the factors that most influenced the structure of the microbial community. Trichoderma harzianum ESALQ-1306 and T. asperellum BRM-29104 promote the biological control of white mold without deleterious effects on the edaphic and endophytic bean communities. |
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Introduction of Trichoderma spp. biocontrol strains against Sclerotinia sclerotiorum (Lib.) de Bary change soil microbial community composition in common bean (Phaseolus vulgaris L.) cultivationBiocontrolMetagenomicsMicrobial ecologyNon-targetBiological control represents a promising alternative to maintain the viability and productivity of different agricultural crops. In this work, dry bean (Phaseolus vulgaris L.) was grown in a field contaminated with Sclerotinia sclerotiorum (Lib.) de Bary, the causal agent of white mold disease. We analyzed the impact of the application of antagonistic strains of Trichoderma harzianum ESALQ-1306 and Trichoderma asperellum BRM-29104 on the edaphic native microbiota and endophytic fungal community. The structure of the fungal and bacterial communities was evaluated by next generation sequencing of the 28S and 16S rRNA genes, respectively. The results showed that the introduction of the strains changed the composition of the community without significantly affecting the parameters of abundance and alpha diversity (Anova P > 0.05). Ascomycota (54.8%) and Actinobacteria (37.2%) were predominant in the analyzed communities. Hill's diversity profiles of the treatments were similar in terms of diversity and dominance, being higher in a 90-day period and in the control treatment. NMDS and UPGMA analysis show that treatments of different lengths have low similarity in their composition, however, treatments where Trichoderma spp. were inoculated were relatively similar to the control. T. harzianum ESALQ-1306 promoted a greater quantity of indicator OTUs, 17 in total. T. harzianum ESALQ-1306 and T. asperellum BRM-29104 alter the size (1140 and 449 nodes respectively), connectivity parameters such as average degree (79.28 and 22.29 links respectively) and predominant topological roles in co-occurrence networks, but they do not alter the richness or abundance of orthologous genes. Cultivation time and plant organs were the factors that most influenced the structure of the microbial community. Trichoderma harzianum ESALQ-1306 and T. asperellum BRM-29104 promote the biological control of white mold without deleterious effects on the edaphic and endophytic bean communities.Departamento de Botânica e Ecologia Instituto de Biociências Universidade Federal de Mato GrossoDepartamento de Tecnologia Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual PaulistaDepartamento de Tecnologia Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual PaulistaUniversidade Federal de Mato GrossoUniversidade Estadual Paulista (UNESP)de Azevedo Silva, Fábiode Oliveira Vieira, VandinelmaCorreia da Silva, Rafael [UNESP]Guariz Pinheiro, Daniel [UNESP]Antônio Soares, Marcos2022-04-29T08:33:03Z2022-04-29T08:33:03Z2021-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.biocontrol.2021.104755Biological Control, v. 163.1049-9644http://hdl.handle.net/11449/22951310.1016/j.biocontrol.2021.1047552-s2.0-85114841545Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiological Controlinfo:eu-repo/semantics/openAccess2024-06-07T15:32:00Zoai:repositorio.unesp.br:11449/229513Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:19:37.161726Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Introduction of Trichoderma spp. biocontrol strains against Sclerotinia sclerotiorum (Lib.) de Bary change soil microbial community composition in common bean (Phaseolus vulgaris L.) cultivation |
title |
Introduction of Trichoderma spp. biocontrol strains against Sclerotinia sclerotiorum (Lib.) de Bary change soil microbial community composition in common bean (Phaseolus vulgaris L.) cultivation |
spellingShingle |
Introduction of Trichoderma spp. biocontrol strains against Sclerotinia sclerotiorum (Lib.) de Bary change soil microbial community composition in common bean (Phaseolus vulgaris L.) cultivation de Azevedo Silva, Fábio Biocontrol Metagenomics Microbial ecology Non-target |
title_short |
Introduction of Trichoderma spp. biocontrol strains against Sclerotinia sclerotiorum (Lib.) de Bary change soil microbial community composition in common bean (Phaseolus vulgaris L.) cultivation |
title_full |
Introduction of Trichoderma spp. biocontrol strains against Sclerotinia sclerotiorum (Lib.) de Bary change soil microbial community composition in common bean (Phaseolus vulgaris L.) cultivation |
title_fullStr |
Introduction of Trichoderma spp. biocontrol strains against Sclerotinia sclerotiorum (Lib.) de Bary change soil microbial community composition in common bean (Phaseolus vulgaris L.) cultivation |
title_full_unstemmed |
Introduction of Trichoderma spp. biocontrol strains against Sclerotinia sclerotiorum (Lib.) de Bary change soil microbial community composition in common bean (Phaseolus vulgaris L.) cultivation |
title_sort |
Introduction of Trichoderma spp. biocontrol strains against Sclerotinia sclerotiorum (Lib.) de Bary change soil microbial community composition in common bean (Phaseolus vulgaris L.) cultivation |
author |
de Azevedo Silva, Fábio |
author_facet |
de Azevedo Silva, Fábio de Oliveira Vieira, Vandinelma Correia da Silva, Rafael [UNESP] Guariz Pinheiro, Daniel [UNESP] Antônio Soares, Marcos |
author_role |
author |
author2 |
de Oliveira Vieira, Vandinelma Correia da Silva, Rafael [UNESP] Guariz Pinheiro, Daniel [UNESP] Antônio Soares, Marcos |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Mato Grosso Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
de Azevedo Silva, Fábio de Oliveira Vieira, Vandinelma Correia da Silva, Rafael [UNESP] Guariz Pinheiro, Daniel [UNESP] Antônio Soares, Marcos |
dc.subject.por.fl_str_mv |
Biocontrol Metagenomics Microbial ecology Non-target |
topic |
Biocontrol Metagenomics Microbial ecology Non-target |
description |
Biological control represents a promising alternative to maintain the viability and productivity of different agricultural crops. In this work, dry bean (Phaseolus vulgaris L.) was grown in a field contaminated with Sclerotinia sclerotiorum (Lib.) de Bary, the causal agent of white mold disease. We analyzed the impact of the application of antagonistic strains of Trichoderma harzianum ESALQ-1306 and Trichoderma asperellum BRM-29104 on the edaphic native microbiota and endophytic fungal community. The structure of the fungal and bacterial communities was evaluated by next generation sequencing of the 28S and 16S rRNA genes, respectively. The results showed that the introduction of the strains changed the composition of the community without significantly affecting the parameters of abundance and alpha diversity (Anova P > 0.05). Ascomycota (54.8%) and Actinobacteria (37.2%) were predominant in the analyzed communities. Hill's diversity profiles of the treatments were similar in terms of diversity and dominance, being higher in a 90-day period and in the control treatment. NMDS and UPGMA analysis show that treatments of different lengths have low similarity in their composition, however, treatments where Trichoderma spp. were inoculated were relatively similar to the control. T. harzianum ESALQ-1306 promoted a greater quantity of indicator OTUs, 17 in total. T. harzianum ESALQ-1306 and T. asperellum BRM-29104 alter the size (1140 and 449 nodes respectively), connectivity parameters such as average degree (79.28 and 22.29 links respectively) and predominant topological roles in co-occurrence networks, but they do not alter the richness or abundance of orthologous genes. Cultivation time and plant organs were the factors that most influenced the structure of the microbial community. Trichoderma harzianum ESALQ-1306 and T. asperellum BRM-29104 promote the biological control of white mold without deleterious effects on the edaphic and endophytic bean communities. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-11-01 2022-04-29T08:33:03Z 2022-04-29T08:33:03Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.biocontrol.2021.104755 Biological Control, v. 163. 1049-9644 http://hdl.handle.net/11449/229513 10.1016/j.biocontrol.2021.104755 2-s2.0-85114841545 |
url |
http://dx.doi.org/10.1016/j.biocontrol.2021.104755 http://hdl.handle.net/11449/229513 |
identifier_str_mv |
Biological Control, v. 163. 1049-9644 10.1016/j.biocontrol.2021.104755 2-s2.0-85114841545 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biological Control |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129052338290688 |