Lps-induced alterations in reproductive organs and liver pigmentation in the toad rhinella diptycha (Bufonidae)
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1139/cjz-2018-0012 http://hdl.handle.net/11449/188463 |
Resumo: | Anurans can be naturally infected by the bacterium Escherichia coli (Migula, 1895) Castellani and Chalmers, 1919, which contain lipopolysaccharides (LPS) in their external membrane. LPS affects reproductive functions in mammals and extracuta-neous pigmentary system of anurans. Besides the functional gonad, bufonid amphibians have an undeveloped female organ (Bidder’s organ), whose function is poorly known. We aimed to investigate the effects of LPS on testis and Bidder’s organ germ cells and on liver pigmentation in the toad Rhinella diptycha (Cope, 1862). Animals were inoculated with 0.1 mL of saline solution (control) or LPS solution (18 mg/kg) and then euthanized after 24 or 48 h. The organs were processed for histology and then evaluated with morphometric analysis. The LPS decreased the area of bundle spermatozoa and increased lumen spermatozoa, while in Bidder’s organ, the number of degenerating oocytes decreased. Liver melanin increased after 24 h and decreased after 48 h. Our results suggest that LPS delays the release of spermatozoa, possibly due to alterations in serum levels of testosterone and (or) gonadotropins, which may also explain fewer degrading cells in Bidder’s organ. Increased melanin in the liver was expected due to the bactericidal role of this pigment. Thus, LPS affected reproductive organs and induced cytoprotective responses in the liver of amphibians. |
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Lps-induced alterations in reproductive organs and liver pigmentation in the toad rhinella diptycha (Bufonidae)AmphibiaBidderian oogenesisCururu toadLPS infectionMelanomacrophagesRhinella diptychaSpermatogenesisAnurans can be naturally infected by the bacterium Escherichia coli (Migula, 1895) Castellani and Chalmers, 1919, which contain lipopolysaccharides (LPS) in their external membrane. LPS affects reproductive functions in mammals and extracuta-neous pigmentary system of anurans. Besides the functional gonad, bufonid amphibians have an undeveloped female organ (Bidder’s organ), whose function is poorly known. We aimed to investigate the effects of LPS on testis and Bidder’s organ germ cells and on liver pigmentation in the toad Rhinella diptycha (Cope, 1862). Animals were inoculated with 0.1 mL of saline solution (control) or LPS solution (18 mg/kg) and then euthanized after 24 or 48 h. The organs were processed for histology and then evaluated with morphometric analysis. The LPS decreased the area of bundle spermatozoa and increased lumen spermatozoa, while in Bidder’s organ, the number of degenerating oocytes decreased. Liver melanin increased after 24 h and decreased after 48 h. Our results suggest that LPS delays the release of spermatozoa, possibly due to alterations in serum levels of testosterone and (or) gonadotropins, which may also explain fewer degrading cells in Bidder’s organ. Increased melanin in the liver was expected due to the bactericidal role of this pigment. Thus, LPS affected reproductive organs and induced cytoprotective responses in the liver of amphibians.Department of Biology São Paulo State University (Unesp) IbilceDepartment of Hydraulic and Sanitation Center of Ecotoxicology and Applied Ecology School of Engineering of São Carlos University of São Paulo (USP)Department of Biology São Paulo State University (Unesp) IbilceUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Gregorio, L. S. [UNESP]Freitas, J. S.Franco-Belussi, L. [UNESP]Oliveira, C. De [UNESP]2019-10-06T16:08:53Z2019-10-06T16:08:53Z2018-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1336-1345http://dx.doi.org/10.1139/cjz-2018-0012Canadian Journal of Zoology, v. 96, n. 12, p. 1336-1345, 2018.1480-32830008-4301http://hdl.handle.net/11449/18846310.1139/cjz-2018-00122-s2.0-85058039417Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCanadian Journal of Zoologyinfo:eu-repo/semantics/openAccess2021-10-22T22:17:26Zoai:repositorio.unesp.br:11449/188463Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:50:01.030695Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Lps-induced alterations in reproductive organs and liver pigmentation in the toad rhinella diptycha (Bufonidae) |
title |
Lps-induced alterations in reproductive organs and liver pigmentation in the toad rhinella diptycha (Bufonidae) |
spellingShingle |
Lps-induced alterations in reproductive organs and liver pigmentation in the toad rhinella diptycha (Bufonidae) Gregorio, L. S. [UNESP] Amphibia Bidderian oogenesis Cururu toad LPS infection Melanomacrophages Rhinella diptycha Spermatogenesis |
title_short |
Lps-induced alterations in reproductive organs and liver pigmentation in the toad rhinella diptycha (Bufonidae) |
title_full |
Lps-induced alterations in reproductive organs and liver pigmentation in the toad rhinella diptycha (Bufonidae) |
title_fullStr |
Lps-induced alterations in reproductive organs and liver pigmentation in the toad rhinella diptycha (Bufonidae) |
title_full_unstemmed |
Lps-induced alterations in reproductive organs and liver pigmentation in the toad rhinella diptycha (Bufonidae) |
title_sort |
Lps-induced alterations in reproductive organs and liver pigmentation in the toad rhinella diptycha (Bufonidae) |
author |
Gregorio, L. S. [UNESP] |
author_facet |
Gregorio, L. S. [UNESP] Freitas, J. S. Franco-Belussi, L. [UNESP] Oliveira, C. De [UNESP] |
author_role |
author |
author2 |
Freitas, J. S. Franco-Belussi, L. [UNESP] Oliveira, C. De [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Gregorio, L. S. [UNESP] Freitas, J. S. Franco-Belussi, L. [UNESP] Oliveira, C. De [UNESP] |
dc.subject.por.fl_str_mv |
Amphibia Bidderian oogenesis Cururu toad LPS infection Melanomacrophages Rhinella diptycha Spermatogenesis |
topic |
Amphibia Bidderian oogenesis Cururu toad LPS infection Melanomacrophages Rhinella diptycha Spermatogenesis |
description |
Anurans can be naturally infected by the bacterium Escherichia coli (Migula, 1895) Castellani and Chalmers, 1919, which contain lipopolysaccharides (LPS) in their external membrane. LPS affects reproductive functions in mammals and extracuta-neous pigmentary system of anurans. Besides the functional gonad, bufonid amphibians have an undeveloped female organ (Bidder’s organ), whose function is poorly known. We aimed to investigate the effects of LPS on testis and Bidder’s organ germ cells and on liver pigmentation in the toad Rhinella diptycha (Cope, 1862). Animals were inoculated with 0.1 mL of saline solution (control) or LPS solution (18 mg/kg) and then euthanized after 24 or 48 h. The organs were processed for histology and then evaluated with morphometric analysis. The LPS decreased the area of bundle spermatozoa and increased lumen spermatozoa, while in Bidder’s organ, the number of degenerating oocytes decreased. Liver melanin increased after 24 h and decreased after 48 h. Our results suggest that LPS delays the release of spermatozoa, possibly due to alterations in serum levels of testosterone and (or) gonadotropins, which may also explain fewer degrading cells in Bidder’s organ. Increased melanin in the liver was expected due to the bactericidal role of this pigment. Thus, LPS affected reproductive organs and induced cytoprotective responses in the liver of amphibians. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-01 2019-10-06T16:08:53Z 2019-10-06T16:08:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1139/cjz-2018-0012 Canadian Journal of Zoology, v. 96, n. 12, p. 1336-1345, 2018. 1480-3283 0008-4301 http://hdl.handle.net/11449/188463 10.1139/cjz-2018-0012 2-s2.0-85058039417 |
url |
http://dx.doi.org/10.1139/cjz-2018-0012 http://hdl.handle.net/11449/188463 |
identifier_str_mv |
Canadian Journal of Zoology, v. 96, n. 12, p. 1336-1345, 2018. 1480-3283 0008-4301 10.1139/cjz-2018-0012 2-s2.0-85058039417 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Canadian Journal of Zoology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1336-1345 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128987290927104 |