Maternal dietary zinc status alters offspring female mammary gland development and response to acute 7,12-dimethylbenzanthracene insult
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1080/10520295.2023.2196092 http://hdl.handle.net/11449/248680 |
Resumo: | We evaluated the effects of prenatal and postnatal dietary zinc (Zn) deficiency or supplementation on mammary gland morphology and on acute response to 7,12-dimethylbenzanthracene (DMBA) in pubertal female rats. On gestational day 10 (GD 10), rat dams were allocated randomly into three experimental groups of 10: a Zn-adequate diet group (ZnA) fed 35 mg Zn/kg chow, a Zn-deficient diet group (ZnD) fed 3 mg ZN/kg chow and a Zn-supplemented diet group (ZnS) fed 180 mg Zn/kg chow. After weaning, female offspring were fed the same diet as their dams until postnatal day 53 (PND 53). All animals received a single 50 mg/kg dose of DMBA on PND 51 and were euthanized on PND 53. Female ZnD offspring exhibited significantly less weight gain compared to the ZnA group and reduced mammary gland development compared to the ZnD and ZnA groups. By PND 53, the Ki-67 labeling index in mammary gland epithelial cells was significantly greater for the ZnS group than for the ZnA and ZnD groups. Apoptosis and ER-α indices did not differ among groups. The ZnD group exhibited significantly increased lipid hydroperoxide (LOOH) levels and decreased catalase and glutathione peroxidase (GSH-Px) activity compared to the ZnA and ZnS groups. The ZnS group exhibited significantly reduced superoxide dismutase (SOD) activity compared to the ZnA and ZnS groups. We observed atypical ductal hyperplasia in the mammary gland of female ZnS group offspring compared to the ZnA and ZnD groups and decreased expression of the Api5 and Ercc1 genes related to apoptosis inhibition and DNA damage repair, respectively. Both the Zn-deficient and Zn-supplemented diet exerted adverse effects on offspring mammary gland morphology and acute response to DMBA. |
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Maternal dietary zinc status alters offspring female mammary gland development and response to acute 7,12-dimethylbenzanthracene insult7,12-dimethylbenzanthraceneDevelopmentmammary glandratszincWe evaluated the effects of prenatal and postnatal dietary zinc (Zn) deficiency or supplementation on mammary gland morphology and on acute response to 7,12-dimethylbenzanthracene (DMBA) in pubertal female rats. On gestational day 10 (GD 10), rat dams were allocated randomly into three experimental groups of 10: a Zn-adequate diet group (ZnA) fed 35 mg Zn/kg chow, a Zn-deficient diet group (ZnD) fed 3 mg ZN/kg chow and a Zn-supplemented diet group (ZnS) fed 180 mg Zn/kg chow. After weaning, female offspring were fed the same diet as their dams until postnatal day 53 (PND 53). All animals received a single 50 mg/kg dose of DMBA on PND 51 and were euthanized on PND 53. Female ZnD offspring exhibited significantly less weight gain compared to the ZnA group and reduced mammary gland development compared to the ZnD and ZnA groups. By PND 53, the Ki-67 labeling index in mammary gland epithelial cells was significantly greater for the ZnS group than for the ZnA and ZnD groups. Apoptosis and ER-α indices did not differ among groups. The ZnD group exhibited significantly increased lipid hydroperoxide (LOOH) levels and decreased catalase and glutathione peroxidase (GSH-Px) activity compared to the ZnA and ZnS groups. The ZnS group exhibited significantly reduced superoxide dismutase (SOD) activity compared to the ZnA and ZnS groups. We observed atypical ductal hyperplasia in the mammary gland of female ZnS group offspring compared to the ZnA and ZnD groups and decreased expression of the Api5 and Ercc1 genes related to apoptosis inhibition and DNA damage repair, respectively. Both the Zn-deficient and Zn-supplemented diet exerted adverse effects on offspring mammary gland morphology and acute response to DMBA.Faculty of Pharmaceutical Sciences Campinas University, SPDepartment of Structural and Functional Biology Institute of Biosciences São Paulo State University, SPDepartment of Pathology School of Medicine São Paulo State UniversityMolecular Oncology Research Center Barretos Cancer Hospital, SPDr. Paulo Prata-FACISB Barretos School of Health Sciences, SPDepartment of Chemistry and Biochemistry Institute of Biosciences São Paulo State University, SPDepartment of Structural and Functional Biology Institute of Biosciences São Paulo State University, SPDepartment of Pathology School of Medicine São Paulo State UniversityDepartment of Chemistry and Biochemistry Institute of Biosciences São Paulo State University, SPUniversidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (UNESP)Barretos Cancer HospitalBarretos School of Health Sciencesda Silva, Flávia R. M.Zapaterini, Joyce R. [UNESP]Grassi, Tony F. [UNESP]Bidinotto, Lucas T. [UNESP]Fernandes, Ana Angélica H. [UNESP]Barbisan, Luis F. [UNESP]2023-07-29T13:50:41Z2023-07-29T13:50:41Z2023-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1080/10520295.2023.2196092Biotechnic and Histochemistry.1473-77601052-0295http://hdl.handle.net/11449/24868010.1080/10520295.2023.21960922-s2.0-85152373801Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiotechnic and Histochemistryinfo:eu-repo/semantics/openAccess2023-07-29T13:50:41Zoai:repositorio.unesp.br:11449/248680Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:35:38.775100Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Maternal dietary zinc status alters offspring female mammary gland development and response to acute 7,12-dimethylbenzanthracene insult |
title |
Maternal dietary zinc status alters offspring female mammary gland development and response to acute 7,12-dimethylbenzanthracene insult |
spellingShingle |
Maternal dietary zinc status alters offspring female mammary gland development and response to acute 7,12-dimethylbenzanthracene insult da Silva, Flávia R. M. 7,12-dimethylbenzanthracene Development mammary gland rats zinc |
title_short |
Maternal dietary zinc status alters offspring female mammary gland development and response to acute 7,12-dimethylbenzanthracene insult |
title_full |
Maternal dietary zinc status alters offspring female mammary gland development and response to acute 7,12-dimethylbenzanthracene insult |
title_fullStr |
Maternal dietary zinc status alters offspring female mammary gland development and response to acute 7,12-dimethylbenzanthracene insult |
title_full_unstemmed |
Maternal dietary zinc status alters offspring female mammary gland development and response to acute 7,12-dimethylbenzanthracene insult |
title_sort |
Maternal dietary zinc status alters offspring female mammary gland development and response to acute 7,12-dimethylbenzanthracene insult |
author |
da Silva, Flávia R. M. |
author_facet |
da Silva, Flávia R. M. Zapaterini, Joyce R. [UNESP] Grassi, Tony F. [UNESP] Bidinotto, Lucas T. [UNESP] Fernandes, Ana Angélica H. [UNESP] Barbisan, Luis F. [UNESP] |
author_role |
author |
author2 |
Zapaterini, Joyce R. [UNESP] Grassi, Tony F. [UNESP] Bidinotto, Lucas T. [UNESP] Fernandes, Ana Angélica H. [UNESP] Barbisan, Luis F. [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Universidade Estadual Paulista (UNESP) Barretos Cancer Hospital Barretos School of Health Sciences |
dc.contributor.author.fl_str_mv |
da Silva, Flávia R. M. Zapaterini, Joyce R. [UNESP] Grassi, Tony F. [UNESP] Bidinotto, Lucas T. [UNESP] Fernandes, Ana Angélica H. [UNESP] Barbisan, Luis F. [UNESP] |
dc.subject.por.fl_str_mv |
7,12-dimethylbenzanthracene Development mammary gland rats zinc |
topic |
7,12-dimethylbenzanthracene Development mammary gland rats zinc |
description |
We evaluated the effects of prenatal and postnatal dietary zinc (Zn) deficiency or supplementation on mammary gland morphology and on acute response to 7,12-dimethylbenzanthracene (DMBA) in pubertal female rats. On gestational day 10 (GD 10), rat dams were allocated randomly into three experimental groups of 10: a Zn-adequate diet group (ZnA) fed 35 mg Zn/kg chow, a Zn-deficient diet group (ZnD) fed 3 mg ZN/kg chow and a Zn-supplemented diet group (ZnS) fed 180 mg Zn/kg chow. After weaning, female offspring were fed the same diet as their dams until postnatal day 53 (PND 53). All animals received a single 50 mg/kg dose of DMBA on PND 51 and were euthanized on PND 53. Female ZnD offspring exhibited significantly less weight gain compared to the ZnA group and reduced mammary gland development compared to the ZnD and ZnA groups. By PND 53, the Ki-67 labeling index in mammary gland epithelial cells was significantly greater for the ZnS group than for the ZnA and ZnD groups. Apoptosis and ER-α indices did not differ among groups. The ZnD group exhibited significantly increased lipid hydroperoxide (LOOH) levels and decreased catalase and glutathione peroxidase (GSH-Px) activity compared to the ZnA and ZnS groups. The ZnS group exhibited significantly reduced superoxide dismutase (SOD) activity compared to the ZnA and ZnS groups. We observed atypical ductal hyperplasia in the mammary gland of female ZnS group offspring compared to the ZnA and ZnD groups and decreased expression of the Api5 and Ercc1 genes related to apoptosis inhibition and DNA damage repair, respectively. Both the Zn-deficient and Zn-supplemented diet exerted adverse effects on offspring mammary gland morphology and acute response to DMBA. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T13:50:41Z 2023-07-29T13:50:41Z 2023-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1080/10520295.2023.2196092 Biotechnic and Histochemistry. 1473-7760 1052-0295 http://hdl.handle.net/11449/248680 10.1080/10520295.2023.2196092 2-s2.0-85152373801 |
url |
http://dx.doi.org/10.1080/10520295.2023.2196092 http://hdl.handle.net/11449/248680 |
identifier_str_mv |
Biotechnic and Histochemistry. 1473-7760 1052-0295 10.1080/10520295.2023.2196092 2-s2.0-85152373801 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biotechnic and Histochemistry |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128953260441600 |