Maternal dietary zinc status alters offspring female mammary gland development and response to acute 7,12-dimethylbenzanthracene insult

Detalhes bibliográficos
Autor(a) principal: da Silva, Flávia R. M.
Data de Publicação: 2023
Outros Autores: Zapaterini, Joyce R. [UNESP], Grassi, Tony F. [UNESP], Bidinotto, Lucas T. [UNESP], Fernandes, Ana Angélica H. [UNESP], Barbisan, Luis F. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1080/10520295.2023.2196092
http://hdl.handle.net/11449/248680
Resumo: We evaluated the effects of prenatal and postnatal dietary zinc (Zn) deficiency or supplementation on mammary gland morphology and on acute response to 7,12-dimethylbenzanthracene (DMBA) in pubertal female rats. On gestational day 10 (GD 10), rat dams were allocated randomly into three experimental groups of 10: a Zn-adequate diet group (ZnA) fed 35 mg Zn/kg chow, a Zn-deficient diet group (ZnD) fed 3 mg ZN/kg chow and a Zn-supplemented diet group (ZnS) fed 180 mg Zn/kg chow. After weaning, female offspring were fed the same diet as their dams until postnatal day 53 (PND 53). All animals received a single 50 mg/kg dose of DMBA on PND 51 and were euthanized on PND 53. Female ZnD offspring exhibited significantly less weight gain compared to the ZnA group and reduced mammary gland development compared to the ZnD and ZnA groups. By PND 53, the Ki-67 labeling index in mammary gland epithelial cells was significantly greater for the ZnS group than for the ZnA and ZnD groups. Apoptosis and ER-α indices did not differ among groups. The ZnD group exhibited significantly increased lipid hydroperoxide (LOOH) levels and decreased catalase and glutathione peroxidase (GSH-Px) activity compared to the ZnA and ZnS groups. The ZnS group exhibited significantly reduced superoxide dismutase (SOD) activity compared to the ZnA and ZnS groups. We observed atypical ductal hyperplasia in the mammary gland of female ZnS group offspring compared to the ZnA and ZnD groups and decreased expression of the Api5 and Ercc1 genes related to apoptosis inhibition and DNA damage repair, respectively. Both the Zn-deficient and Zn-supplemented diet exerted adverse effects on offspring mammary gland morphology and acute response to DMBA.
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spelling Maternal dietary zinc status alters offspring female mammary gland development and response to acute 7,12-dimethylbenzanthracene insult7,12-dimethylbenzanthraceneDevelopmentmammary glandratszincWe evaluated the effects of prenatal and postnatal dietary zinc (Zn) deficiency or supplementation on mammary gland morphology and on acute response to 7,12-dimethylbenzanthracene (DMBA) in pubertal female rats. On gestational day 10 (GD 10), rat dams were allocated randomly into three experimental groups of 10: a Zn-adequate diet group (ZnA) fed 35 mg Zn/kg chow, a Zn-deficient diet group (ZnD) fed 3 mg ZN/kg chow and a Zn-supplemented diet group (ZnS) fed 180 mg Zn/kg chow. After weaning, female offspring were fed the same diet as their dams until postnatal day 53 (PND 53). All animals received a single 50 mg/kg dose of DMBA on PND 51 and were euthanized on PND 53. Female ZnD offspring exhibited significantly less weight gain compared to the ZnA group and reduced mammary gland development compared to the ZnD and ZnA groups. By PND 53, the Ki-67 labeling index in mammary gland epithelial cells was significantly greater for the ZnS group than for the ZnA and ZnD groups. Apoptosis and ER-α indices did not differ among groups. The ZnD group exhibited significantly increased lipid hydroperoxide (LOOH) levels and decreased catalase and glutathione peroxidase (GSH-Px) activity compared to the ZnA and ZnS groups. The ZnS group exhibited significantly reduced superoxide dismutase (SOD) activity compared to the ZnA and ZnS groups. We observed atypical ductal hyperplasia in the mammary gland of female ZnS group offspring compared to the ZnA and ZnD groups and decreased expression of the Api5 and Ercc1 genes related to apoptosis inhibition and DNA damage repair, respectively. Both the Zn-deficient and Zn-supplemented diet exerted adverse effects on offspring mammary gland morphology and acute response to DMBA.Faculty of Pharmaceutical Sciences Campinas University, SPDepartment of Structural and Functional Biology Institute of Biosciences São Paulo State University, SPDepartment of Pathology School of Medicine São Paulo State UniversityMolecular Oncology Research Center Barretos Cancer Hospital, SPDr. Paulo Prata-FACISB Barretos School of Health Sciences, SPDepartment of Chemistry and Biochemistry Institute of Biosciences São Paulo State University, SPDepartment of Structural and Functional Biology Institute of Biosciences São Paulo State University, SPDepartment of Pathology School of Medicine São Paulo State UniversityDepartment of Chemistry and Biochemistry Institute of Biosciences São Paulo State University, SPUniversidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (UNESP)Barretos Cancer HospitalBarretos School of Health Sciencesda Silva, Flávia R. M.Zapaterini, Joyce R. [UNESP]Grassi, Tony F. [UNESP]Bidinotto, Lucas T. [UNESP]Fernandes, Ana Angélica H. [UNESP]Barbisan, Luis F. [UNESP]2023-07-29T13:50:41Z2023-07-29T13:50:41Z2023-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1080/10520295.2023.2196092Biotechnic and Histochemistry.1473-77601052-0295http://hdl.handle.net/11449/24868010.1080/10520295.2023.21960922-s2.0-85152373801Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiotechnic and Histochemistryinfo:eu-repo/semantics/openAccess2023-07-29T13:50:41Zoai:repositorio.unesp.br:11449/248680Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:35:38.775100Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Maternal dietary zinc status alters offspring female mammary gland development and response to acute 7,12-dimethylbenzanthracene insult
title Maternal dietary zinc status alters offspring female mammary gland development and response to acute 7,12-dimethylbenzanthracene insult
spellingShingle Maternal dietary zinc status alters offspring female mammary gland development and response to acute 7,12-dimethylbenzanthracene insult
da Silva, Flávia R. M.
7,12-dimethylbenzanthracene
Development
mammary gland
rats
zinc
title_short Maternal dietary zinc status alters offspring female mammary gland development and response to acute 7,12-dimethylbenzanthracene insult
title_full Maternal dietary zinc status alters offspring female mammary gland development and response to acute 7,12-dimethylbenzanthracene insult
title_fullStr Maternal dietary zinc status alters offspring female mammary gland development and response to acute 7,12-dimethylbenzanthracene insult
title_full_unstemmed Maternal dietary zinc status alters offspring female mammary gland development and response to acute 7,12-dimethylbenzanthracene insult
title_sort Maternal dietary zinc status alters offspring female mammary gland development and response to acute 7,12-dimethylbenzanthracene insult
author da Silva, Flávia R. M.
author_facet da Silva, Flávia R. M.
Zapaterini, Joyce R. [UNESP]
Grassi, Tony F. [UNESP]
Bidinotto, Lucas T. [UNESP]
Fernandes, Ana Angélica H. [UNESP]
Barbisan, Luis F. [UNESP]
author_role author
author2 Zapaterini, Joyce R. [UNESP]
Grassi, Tony F. [UNESP]
Bidinotto, Lucas T. [UNESP]
Fernandes, Ana Angélica H. [UNESP]
Barbisan, Luis F. [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Campinas (UNICAMP)
Universidade Estadual Paulista (UNESP)
Barretos Cancer Hospital
Barretos School of Health Sciences
dc.contributor.author.fl_str_mv da Silva, Flávia R. M.
Zapaterini, Joyce R. [UNESP]
Grassi, Tony F. [UNESP]
Bidinotto, Lucas T. [UNESP]
Fernandes, Ana Angélica H. [UNESP]
Barbisan, Luis F. [UNESP]
dc.subject.por.fl_str_mv 7,12-dimethylbenzanthracene
Development
mammary gland
rats
zinc
topic 7,12-dimethylbenzanthracene
Development
mammary gland
rats
zinc
description We evaluated the effects of prenatal and postnatal dietary zinc (Zn) deficiency or supplementation on mammary gland morphology and on acute response to 7,12-dimethylbenzanthracene (DMBA) in pubertal female rats. On gestational day 10 (GD 10), rat dams were allocated randomly into three experimental groups of 10: a Zn-adequate diet group (ZnA) fed 35 mg Zn/kg chow, a Zn-deficient diet group (ZnD) fed 3 mg ZN/kg chow and a Zn-supplemented diet group (ZnS) fed 180 mg Zn/kg chow. After weaning, female offspring were fed the same diet as their dams until postnatal day 53 (PND 53). All animals received a single 50 mg/kg dose of DMBA on PND 51 and were euthanized on PND 53. Female ZnD offspring exhibited significantly less weight gain compared to the ZnA group and reduced mammary gland development compared to the ZnD and ZnA groups. By PND 53, the Ki-67 labeling index in mammary gland epithelial cells was significantly greater for the ZnS group than for the ZnA and ZnD groups. Apoptosis and ER-α indices did not differ among groups. The ZnD group exhibited significantly increased lipid hydroperoxide (LOOH) levels and decreased catalase and glutathione peroxidase (GSH-Px) activity compared to the ZnA and ZnS groups. The ZnS group exhibited significantly reduced superoxide dismutase (SOD) activity compared to the ZnA and ZnS groups. We observed atypical ductal hyperplasia in the mammary gland of female ZnS group offspring compared to the ZnA and ZnD groups and decreased expression of the Api5 and Ercc1 genes related to apoptosis inhibition and DNA damage repair, respectively. Both the Zn-deficient and Zn-supplemented diet exerted adverse effects on offspring mammary gland morphology and acute response to DMBA.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-29T13:50:41Z
2023-07-29T13:50:41Z
2023-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1080/10520295.2023.2196092
Biotechnic and Histochemistry.
1473-7760
1052-0295
http://hdl.handle.net/11449/248680
10.1080/10520295.2023.2196092
2-s2.0-85152373801
url http://dx.doi.org/10.1080/10520295.2023.2196092
http://hdl.handle.net/11449/248680
identifier_str_mv Biotechnic and Histochemistry.
1473-7760
1052-0295
10.1080/10520295.2023.2196092
2-s2.0-85152373801
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Biotechnic and Histochemistry
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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