Decontamination of root canals infected with reciprocating instruments, sodium hypochlorite 2.5% and apple vinegar
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.14436/2358-2545.8.2.070-077.oar http://hdl.handle.net/11449/221265 |
Resumo: | Objective: To evaluate decontamination of root canals infected with reciprocating instruments, 2.5% sodium hypochlorite and apple vinegar. Methods: A total of 50 single- rooted human teeth were emptied and contaminated with Enterococcus faecalis for 60 days. After the period of contamination, root canal preparation was performed with instruments, including: 1. WaveOne TM 40.08 (n = 10); 2. Reciproc TM 40.06 (n = 10); and 3. Unicone TM 40.06 (n = 10). Two control groups were used: positive control (n = 10) and negative control (n = 10). Half of the samples from each group were irrigated with 2.5% sodium hypochlorite, while another half was irrigated with apple cider vinegar. Subsequently, sterilized paper cup collections were made and immersed in culture medium with 7 mL of Lethen Broth and incubated at 37 °C for 48 hours in a reduced oxygen atmosphere. Bacterial growth was analyzed by turbidity of culture medium and UV spectrophotometry. Cleaning of dentine walls was analyzed by scanning electron microscopy. Results: All groups showed reduction of optical density of the culture medium after root canal preparation (p < 0.05). No sanitation strategy promoted complete elimination of the biological indicator. Complete removal of debris occurred in none of the groups, and no significant differences were found regardless of the thirds analyzed (p > 0.05). Conclusion: Tested reciprocating instruments and irrigating solutions reduced bacterial contamination of root canals infected with E. faecalis. |
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Decontamination of root canals infected with reciprocating instruments, sodium hypochlorite 2.5% and apple vinegarRoot canal irrigating solutionsRoot canal preparationRoot canal therapyObjective: To evaluate decontamination of root canals infected with reciprocating instruments, 2.5% sodium hypochlorite and apple vinegar. Methods: A total of 50 single- rooted human teeth were emptied and contaminated with Enterococcus faecalis for 60 days. After the period of contamination, root canal preparation was performed with instruments, including: 1. WaveOne TM 40.08 (n = 10); 2. Reciproc TM 40.06 (n = 10); and 3. Unicone TM 40.06 (n = 10). Two control groups were used: positive control (n = 10) and negative control (n = 10). Half of the samples from each group were irrigated with 2.5% sodium hypochlorite, while another half was irrigated with apple cider vinegar. Subsequently, sterilized paper cup collections were made and immersed in culture medium with 7 mL of Lethen Broth and incubated at 37 °C for 48 hours in a reduced oxygen atmosphere. Bacterial growth was analyzed by turbidity of culture medium and UV spectrophotometry. Cleaning of dentine walls was analyzed by scanning electron microscopy. Results: All groups showed reduction of optical density of the culture medium after root canal preparation (p < 0.05). No sanitation strategy promoted complete elimination of the biological indicator. Complete removal of debris occurred in none of the groups, and no significant differences were found regardless of the thirds analyzed (p > 0.05). Conclusion: Tested reciprocating instruments and irrigating solutions reduced bacterial contamination of root canals infected with E. faecalis.UniEvangélica Centro Universitário de Anápolis Departamento de EndodontiaUniversidade Federal de Goiás Faculdade de MedicinaUniversidade Federal de Goiás Faculdade de Odontologia Departamento de EndodontiaUniversidade Estadual Paulista Júlio de Mesquita FilhoUniversidade de Cuiabá Programa de Pós-graduação em Ciências Odontológicas IntegradasUniversidade Federal de Goiás Instituto de Ciências BiológicasUniversidade Federal de Goiás Faculdade de Odontologia Disciplinas de Endodontia e UrgênciaUniversidade Federal de Goiás Programa de Pós-graduação em Ciências da SaúdeUniversidade de São Paulo Faculdade de Odontologia de Ribeirão PretoUniversidade Estadual Paulista Júlio de Mesquita FilhoUniEvangélica Centro Universitário de AnápolisUniversidade Federal de Goiás (UFG)Universidade Estadual Paulista (UNESP)Programa de Pós-graduação em Ciências Odontológicas IntegradasUniversidade de São Paulo (USP)de Oliveira, Helder FernandesAlencar, Ana Helena Gonçalves [UNESP]Estrela, Cyntia Rodrigues de AraújoDecurcio, Daniel AlmeidaSilva, Julio AlmeidaSousa, Vinícius CaixetaEstrela, Carlos2022-04-28T19:27:03Z2022-04-28T19:27:03Z2018-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article70-77http://dx.doi.org/10.14436/2358-2545.8.2.070-077.oarDental Press Endodontics, v. 8, n. 2, p. 70-77, 2018.2178-3713http://hdl.handle.net/11449/22126510.14436/2358-2545.8.2.070-077.oar2-s2.0-85062985784Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengDental Press Endodonticsinfo:eu-repo/semantics/openAccess2022-04-28T19:27:03Zoai:repositorio.unesp.br:11449/221265Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:17:04.925970Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Decontamination of root canals infected with reciprocating instruments, sodium hypochlorite 2.5% and apple vinegar |
title |
Decontamination of root canals infected with reciprocating instruments, sodium hypochlorite 2.5% and apple vinegar |
spellingShingle |
Decontamination of root canals infected with reciprocating instruments, sodium hypochlorite 2.5% and apple vinegar de Oliveira, Helder Fernandes Root canal irrigating solutions Root canal preparation Root canal therapy |
title_short |
Decontamination of root canals infected with reciprocating instruments, sodium hypochlorite 2.5% and apple vinegar |
title_full |
Decontamination of root canals infected with reciprocating instruments, sodium hypochlorite 2.5% and apple vinegar |
title_fullStr |
Decontamination of root canals infected with reciprocating instruments, sodium hypochlorite 2.5% and apple vinegar |
title_full_unstemmed |
Decontamination of root canals infected with reciprocating instruments, sodium hypochlorite 2.5% and apple vinegar |
title_sort |
Decontamination of root canals infected with reciprocating instruments, sodium hypochlorite 2.5% and apple vinegar |
author |
de Oliveira, Helder Fernandes |
author_facet |
de Oliveira, Helder Fernandes Alencar, Ana Helena Gonçalves [UNESP] Estrela, Cyntia Rodrigues de Araújo Decurcio, Daniel Almeida Silva, Julio Almeida Sousa, Vinícius Caixeta Estrela, Carlos |
author_role |
author |
author2 |
Alencar, Ana Helena Gonçalves [UNESP] Estrela, Cyntia Rodrigues de Araújo Decurcio, Daniel Almeida Silva, Julio Almeida Sousa, Vinícius Caixeta Estrela, Carlos |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
UniEvangélica Centro Universitário de Anápolis Universidade Federal de Goiás (UFG) Universidade Estadual Paulista (UNESP) Programa de Pós-graduação em Ciências Odontológicas Integradas Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
de Oliveira, Helder Fernandes Alencar, Ana Helena Gonçalves [UNESP] Estrela, Cyntia Rodrigues de Araújo Decurcio, Daniel Almeida Silva, Julio Almeida Sousa, Vinícius Caixeta Estrela, Carlos |
dc.subject.por.fl_str_mv |
Root canal irrigating solutions Root canal preparation Root canal therapy |
topic |
Root canal irrigating solutions Root canal preparation Root canal therapy |
description |
Objective: To evaluate decontamination of root canals infected with reciprocating instruments, 2.5% sodium hypochlorite and apple vinegar. Methods: A total of 50 single- rooted human teeth were emptied and contaminated with Enterococcus faecalis for 60 days. After the period of contamination, root canal preparation was performed with instruments, including: 1. WaveOne TM 40.08 (n = 10); 2. Reciproc TM 40.06 (n = 10); and 3. Unicone TM 40.06 (n = 10). Two control groups were used: positive control (n = 10) and negative control (n = 10). Half of the samples from each group were irrigated with 2.5% sodium hypochlorite, while another half was irrigated with apple cider vinegar. Subsequently, sterilized paper cup collections were made and immersed in culture medium with 7 mL of Lethen Broth and incubated at 37 °C for 48 hours in a reduced oxygen atmosphere. Bacterial growth was analyzed by turbidity of culture medium and UV spectrophotometry. Cleaning of dentine walls was analyzed by scanning electron microscopy. Results: All groups showed reduction of optical density of the culture medium after root canal preparation (p < 0.05). No sanitation strategy promoted complete elimination of the biological indicator. Complete removal of debris occurred in none of the groups, and no significant differences were found regardless of the thirds analyzed (p > 0.05). Conclusion: Tested reciprocating instruments and irrigating solutions reduced bacterial contamination of root canals infected with E. faecalis. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-01 2022-04-28T19:27:03Z 2022-04-28T19:27:03Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.14436/2358-2545.8.2.070-077.oar Dental Press Endodontics, v. 8, n. 2, p. 70-77, 2018. 2178-3713 http://hdl.handle.net/11449/221265 10.14436/2358-2545.8.2.070-077.oar 2-s2.0-85062985784 |
url |
http://dx.doi.org/10.14436/2358-2545.8.2.070-077.oar http://hdl.handle.net/11449/221265 |
identifier_str_mv |
Dental Press Endodontics, v. 8, n. 2, p. 70-77, 2018. 2178-3713 10.14436/2358-2545.8.2.070-077.oar 2-s2.0-85062985784 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Dental Press Endodontics |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
70-77 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129503859310592 |