Potential cannabidiol (CBD) repurposing as antibacterial and promising therapy of CBD plus polymyxin B (PB) against PB-resistant gram-negative bacilli
Autor(a) principal: | |
---|---|
Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1038/s41598-022-10393-8 http://hdl.handle.net/11449/240855 |
Resumo: | This study aimed to assess the ultrapure cannabidiol (CBD) antibacterial activity and to investigate the antibacterial activity of the combination CBD + polymyxin B (PB) against Gram-negative (GN) bacteria, including PB-resistant Gram-negative bacilli (GNB). We used the standard broth microdilution method, checkerboard assay, and time-kill assay. CBD exhibited antibacterial activity against Gram-positive bacteria, lipooligosaccharide (LOS)-expressing GN diplococcus (GND) (Neisseria gonorrhoeae, Neisseria meningitidis, Moraxella catarrhalis), and Mycobacterium tuberculosis, but not against GNB. For most of the GNB studied, our results showed that low concentrations of PB (≤ 2 µg/mL) allow CBD (≤ 4 µg/mL) to exert antibacterial activity against GNB (e.g., Klebsiella pneumoniae, Escherichia coli, Acinetobacter baumannii), including PB-resistant GNB. CBD + PB also showed additive and/or synergistic effect against LOS-expressing GND. Time-kill assays results showed that the combination CBD + PB leads to a greater reduction in the number of colony forming units per milliliter compared to CBD and PB alone, at the same concentration used in combination, and the combination CBD + PB was synergistic for all four PB-resistant K. pneumoniae isolates evaluated. Our results show that CBD has translational potential and should be further explored as a repurposed antibacterial agent in clinical trials. The antibacterial efficacy of the combination CBD + PB against multidrug-resistant and extensively drug-resistant GNB, especially PB-resistant K. pneumoniae, is particularly promising. |
id |
UNSP_8d6727df8768782f133bff7b4d8d85b3 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/240855 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Potential cannabidiol (CBD) repurposing as antibacterial and promising therapy of CBD plus polymyxin B (PB) against PB-resistant gram-negative bacilliThis study aimed to assess the ultrapure cannabidiol (CBD) antibacterial activity and to investigate the antibacterial activity of the combination CBD + polymyxin B (PB) against Gram-negative (GN) bacteria, including PB-resistant Gram-negative bacilli (GNB). We used the standard broth microdilution method, checkerboard assay, and time-kill assay. CBD exhibited antibacterial activity against Gram-positive bacteria, lipooligosaccharide (LOS)-expressing GN diplococcus (GND) (Neisseria gonorrhoeae, Neisseria meningitidis, Moraxella catarrhalis), and Mycobacterium tuberculosis, but not against GNB. For most of the GNB studied, our results showed that low concentrations of PB (≤ 2 µg/mL) allow CBD (≤ 4 µg/mL) to exert antibacterial activity against GNB (e.g., Klebsiella pneumoniae, Escherichia coli, Acinetobacter baumannii), including PB-resistant GNB. CBD + PB also showed additive and/or synergistic effect against LOS-expressing GND. Time-kill assays results showed that the combination CBD + PB leads to a greater reduction in the number of colony forming units per milliliter compared to CBD and PB alone, at the same concentration used in combination, and the combination CBD + PB was synergistic for all four PB-resistant K. pneumoniae isolates evaluated. Our results show that CBD has translational potential and should be further explored as a repurposed antibacterial agent in clinical trials. The antibacterial efficacy of the combination CBD + PB against multidrug-resistant and extensively drug-resistant GNB, especially PB-resistant K. pneumoniae, is particularly promising.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Department of Clinical Analyses Toxicology and Food Science (DACTB) School of Pharmaceutical Sciences of Ribeirao Preto (FCFRP) University of São Paulo (USP), Av. do Café, s/nº, Campus Universitário, SPDepartment of Social Medicine Ribeirão Preto Medical School (FMRP) University of São Paulo (USP), SPDepartment of Biological Sciences School of Pharmaceutical Sciences (FCF) São Paulo State University (UNESP), SPDepartment of Pharmaceutical Sciences (DCF) School of Pharmaceutical Sciences of Ribeirão Preto (FCFRP) University of São Paulo (USP), SPDepartment of Neurosciences and Behavioral Sciences Ribeirão Preto Medical School (FMRP) University of São Paulo (USP), SPNational Institute of Science and Technology for Translational Medicine (INCT-TM) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), DFHospital Universitario Ramón y Cajal and Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS)Department of Biological Sciences School of Pharmaceutical Sciences (FCF) São Paulo State University (UNESP), SPCNPq: 130766/2021-6CAPES: 8887.369851/2019-00Universidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Hospital Universitario Ramón y Cajal and Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS)Abichabki, NatháliaZacharias, Luísa V.Moreira, Natália C.Bellissimo-Rodrigues, FernandoMoreira, Fernanda L.Benzi, Jhohann R. L.Ogasawara, Tânia M. C.Ferreira, Joseane C.Ribeiro, Camila M. [UNESP]Pavan, Fernando R. [UNESP]Pereira, Leonardo R. L.Brancini, Guilherme T. P.Braga, Gilberto Ú. L.Zuardi, Antonio W.Hallak, Jaime E. C.Crippa, José A. S.Lanchote, Vera L.Cantón, RafaelDarini, Ana Lúcia C.Andrade, Leonardo N.2023-03-01T20:35:50Z2023-03-01T20:35:50Z2022-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1038/s41598-022-10393-8Scientific Reports, v. 12, n. 1, 2022.2045-2322http://hdl.handle.net/11449/24085510.1038/s41598-022-10393-82-s2.0-85128444743Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientific Reportsinfo:eu-repo/semantics/openAccess2024-06-24T13:06:58Zoai:repositorio.unesp.br:11449/240855Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:40:59.512895Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Potential cannabidiol (CBD) repurposing as antibacterial and promising therapy of CBD plus polymyxin B (PB) against PB-resistant gram-negative bacilli |
title |
Potential cannabidiol (CBD) repurposing as antibacterial and promising therapy of CBD plus polymyxin B (PB) against PB-resistant gram-negative bacilli |
spellingShingle |
Potential cannabidiol (CBD) repurposing as antibacterial and promising therapy of CBD plus polymyxin B (PB) against PB-resistant gram-negative bacilli Abichabki, Nathália |
title_short |
Potential cannabidiol (CBD) repurposing as antibacterial and promising therapy of CBD plus polymyxin B (PB) against PB-resistant gram-negative bacilli |
title_full |
Potential cannabidiol (CBD) repurposing as antibacterial and promising therapy of CBD plus polymyxin B (PB) against PB-resistant gram-negative bacilli |
title_fullStr |
Potential cannabidiol (CBD) repurposing as antibacterial and promising therapy of CBD plus polymyxin B (PB) against PB-resistant gram-negative bacilli |
title_full_unstemmed |
Potential cannabidiol (CBD) repurposing as antibacterial and promising therapy of CBD plus polymyxin B (PB) against PB-resistant gram-negative bacilli |
title_sort |
Potential cannabidiol (CBD) repurposing as antibacterial and promising therapy of CBD plus polymyxin B (PB) against PB-resistant gram-negative bacilli |
author |
Abichabki, Nathália |
author_facet |
Abichabki, Nathália Zacharias, Luísa V. Moreira, Natália C. Bellissimo-Rodrigues, Fernando Moreira, Fernanda L. Benzi, Jhohann R. L. Ogasawara, Tânia M. C. Ferreira, Joseane C. Ribeiro, Camila M. [UNESP] Pavan, Fernando R. [UNESP] Pereira, Leonardo R. L. Brancini, Guilherme T. P. Braga, Gilberto Ú. L. Zuardi, Antonio W. Hallak, Jaime E. C. Crippa, José A. S. Lanchote, Vera L. Cantón, Rafael Darini, Ana Lúcia C. Andrade, Leonardo N. |
author_role |
author |
author2 |
Zacharias, Luísa V. Moreira, Natália C. Bellissimo-Rodrigues, Fernando Moreira, Fernanda L. Benzi, Jhohann R. L. Ogasawara, Tânia M. C. Ferreira, Joseane C. Ribeiro, Camila M. [UNESP] Pavan, Fernando R. [UNESP] Pereira, Leonardo R. L. Brancini, Guilherme T. P. Braga, Gilberto Ú. L. Zuardi, Antonio W. Hallak, Jaime E. C. Crippa, José A. S. Lanchote, Vera L. Cantón, Rafael Darini, Ana Lúcia C. Andrade, Leonardo N. |
author2_role |
author author author author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (UNESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Hospital Universitario Ramón y Cajal and Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS) |
dc.contributor.author.fl_str_mv |
Abichabki, Nathália Zacharias, Luísa V. Moreira, Natália C. Bellissimo-Rodrigues, Fernando Moreira, Fernanda L. Benzi, Jhohann R. L. Ogasawara, Tânia M. C. Ferreira, Joseane C. Ribeiro, Camila M. [UNESP] Pavan, Fernando R. [UNESP] Pereira, Leonardo R. L. Brancini, Guilherme T. P. Braga, Gilberto Ú. L. Zuardi, Antonio W. Hallak, Jaime E. C. Crippa, José A. S. Lanchote, Vera L. Cantón, Rafael Darini, Ana Lúcia C. Andrade, Leonardo N. |
description |
This study aimed to assess the ultrapure cannabidiol (CBD) antibacterial activity and to investigate the antibacterial activity of the combination CBD + polymyxin B (PB) against Gram-negative (GN) bacteria, including PB-resistant Gram-negative bacilli (GNB). We used the standard broth microdilution method, checkerboard assay, and time-kill assay. CBD exhibited antibacterial activity against Gram-positive bacteria, lipooligosaccharide (LOS)-expressing GN diplococcus (GND) (Neisseria gonorrhoeae, Neisseria meningitidis, Moraxella catarrhalis), and Mycobacterium tuberculosis, but not against GNB. For most of the GNB studied, our results showed that low concentrations of PB (≤ 2 µg/mL) allow CBD (≤ 4 µg/mL) to exert antibacterial activity against GNB (e.g., Klebsiella pneumoniae, Escherichia coli, Acinetobacter baumannii), including PB-resistant GNB. CBD + PB also showed additive and/or synergistic effect against LOS-expressing GND. Time-kill assays results showed that the combination CBD + PB leads to a greater reduction in the number of colony forming units per milliliter compared to CBD and PB alone, at the same concentration used in combination, and the combination CBD + PB was synergistic for all four PB-resistant K. pneumoniae isolates evaluated. Our results show that CBD has translational potential and should be further explored as a repurposed antibacterial agent in clinical trials. The antibacterial efficacy of the combination CBD + PB against multidrug-resistant and extensively drug-resistant GNB, especially PB-resistant K. pneumoniae, is particularly promising. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-12-01 2023-03-01T20:35:50Z 2023-03-01T20:35:50Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1038/s41598-022-10393-8 Scientific Reports, v. 12, n. 1, 2022. 2045-2322 http://hdl.handle.net/11449/240855 10.1038/s41598-022-10393-8 2-s2.0-85128444743 |
url |
http://dx.doi.org/10.1038/s41598-022-10393-8 http://hdl.handle.net/11449/240855 |
identifier_str_mv |
Scientific Reports, v. 12, n. 1, 2022. 2045-2322 10.1038/s41598-022-10393-8 2-s2.0-85128444743 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Scientific Reports |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128399747579904 |