Cytological investigations of the effect of colchicine on meiosis in Lilium hybrid cv. Black Beauty microsporocytes
Autor(a) principal: | |
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Data de Publicação: | 1979 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/BF00293232 http://hdl.handle.net/11449/223899 |
Resumo: | Using colchicine, two methods have been successfully applied for the induction of tetraploid meiocytes at premeiotic mitosis in the near achiasmate diploid hybrid cultivar Black Beauty of Lilium. The experiments were aimed at understanding whether the achiasmate condition is attributable to insufficient homology for effective pairing or to some genie defect in order to interpret the data obtained from molecular studies. Cytological observations demonstrate a strong correlation between the induction of tetraploidy and subsequent chiasma formation. The chiasma frequency per cell in untreated diploid control meiocytes ranged from 0 to 8 (mean 2.25), while in colchicine treated undoubled cells sampled 14±2 days after the start of treatment it ranged from 0 to 2 (mean 0.148). By comparison, the chiasma frequency per cell in colchicine-doubled tetraploid cells ranged from 29 to 51 (mean 42.24), such cells showing complete or near complete bivalent pairing. These results, similar to those reported previously for other higher plant species, demonstrate that the achiasmatic condition in Black Beauty is not due to a genie defect. The methods developed have made possible hitherto inaccessible biochemical analyses of meiocytes that had been treated with colchicine or other chemicals at premeiotic stages. © 1979 Springer-Verlag. |
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Cytological investigations of the effect of colchicine on meiosis in Lilium hybrid cv. Black Beauty microsporocytesUsing colchicine, two methods have been successfully applied for the induction of tetraploid meiocytes at premeiotic mitosis in the near achiasmate diploid hybrid cultivar Black Beauty of Lilium. The experiments were aimed at understanding whether the achiasmate condition is attributable to insufficient homology for effective pairing or to some genie defect in order to interpret the data obtained from molecular studies. Cytological observations demonstrate a strong correlation between the induction of tetraploidy and subsequent chiasma formation. The chiasma frequency per cell in untreated diploid control meiocytes ranged from 0 to 8 (mean 2.25), while in colchicine treated undoubled cells sampled 14±2 days after the start of treatment it ranged from 0 to 2 (mean 0.148). By comparison, the chiasma frequency per cell in colchicine-doubled tetraploid cells ranged from 29 to 51 (mean 42.24), such cells showing complete or near complete bivalent pairing. These results, similar to those reported previously for other higher plant species, demonstrate that the achiasmatic condition in Black Beauty is not due to a genie defect. The methods developed have made possible hitherto inaccessible biochemical analyses of meiocytes that had been treated with colchicine or other chemicals at premeiotic stages. © 1979 Springer-Verlag.Departamento de Morfologia Instituto Básico de Biologia Médica e Agrícola, UNESP, Botucatu, São Paulo, 18600Plant Breeding Institute, Maris Lane, Trumpington, CB2 2LQ, CambridgeDepartment of Biology University of California, San Diego, La Jolla, 92093, CaliforniaDepartamento de Morfologia Instituto Básico de Biologia Médica e Agrícola, UNESP, Botucatu, São Paulo, 18600Universidade Estadual Paulista (UNESP)Plant Breeding InstituteUniversity of CaliforniaToledo, L. A. [UNESP]Bennett, M. D.Stern, H.2022-04-28T19:53:34Z2022-04-28T19:53:34Z1979-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article157-173http://dx.doi.org/10.1007/BF00293232Chromosoma, v. 72, n. 2, p. 157-173, 1979.0009-59151432-0886http://hdl.handle.net/11449/22389910.1007/BF002932322-s2.0-0018296012Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengChromosomainfo:eu-repo/semantics/openAccess2022-04-28T19:53:34Zoai:repositorio.unesp.br:11449/223899Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:36:21.283898Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cytological investigations of the effect of colchicine on meiosis in Lilium hybrid cv. Black Beauty microsporocytes |
title |
Cytological investigations of the effect of colchicine on meiosis in Lilium hybrid cv. Black Beauty microsporocytes |
spellingShingle |
Cytological investigations of the effect of colchicine on meiosis in Lilium hybrid cv. Black Beauty microsporocytes Toledo, L. A. [UNESP] |
title_short |
Cytological investigations of the effect of colchicine on meiosis in Lilium hybrid cv. Black Beauty microsporocytes |
title_full |
Cytological investigations of the effect of colchicine on meiosis in Lilium hybrid cv. Black Beauty microsporocytes |
title_fullStr |
Cytological investigations of the effect of colchicine on meiosis in Lilium hybrid cv. Black Beauty microsporocytes |
title_full_unstemmed |
Cytological investigations of the effect of colchicine on meiosis in Lilium hybrid cv. Black Beauty microsporocytes |
title_sort |
Cytological investigations of the effect of colchicine on meiosis in Lilium hybrid cv. Black Beauty microsporocytes |
author |
Toledo, L. A. [UNESP] |
author_facet |
Toledo, L. A. [UNESP] Bennett, M. D. Stern, H. |
author_role |
author |
author2 |
Bennett, M. D. Stern, H. |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Plant Breeding Institute University of California |
dc.contributor.author.fl_str_mv |
Toledo, L. A. [UNESP] Bennett, M. D. Stern, H. |
description |
Using colchicine, two methods have been successfully applied for the induction of tetraploid meiocytes at premeiotic mitosis in the near achiasmate diploid hybrid cultivar Black Beauty of Lilium. The experiments were aimed at understanding whether the achiasmate condition is attributable to insufficient homology for effective pairing or to some genie defect in order to interpret the data obtained from molecular studies. Cytological observations demonstrate a strong correlation between the induction of tetraploidy and subsequent chiasma formation. The chiasma frequency per cell in untreated diploid control meiocytes ranged from 0 to 8 (mean 2.25), while in colchicine treated undoubled cells sampled 14±2 days after the start of treatment it ranged from 0 to 2 (mean 0.148). By comparison, the chiasma frequency per cell in colchicine-doubled tetraploid cells ranged from 29 to 51 (mean 42.24), such cells showing complete or near complete bivalent pairing. These results, similar to those reported previously for other higher plant species, demonstrate that the achiasmatic condition in Black Beauty is not due to a genie defect. The methods developed have made possible hitherto inaccessible biochemical analyses of meiocytes that had been treated with colchicine or other chemicals at premeiotic stages. © 1979 Springer-Verlag. |
publishDate |
1979 |
dc.date.none.fl_str_mv |
1979-01-01 2022-04-28T19:53:34Z 2022-04-28T19:53:34Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/BF00293232 Chromosoma, v. 72, n. 2, p. 157-173, 1979. 0009-5915 1432-0886 http://hdl.handle.net/11449/223899 10.1007/BF00293232 2-s2.0-0018296012 |
url |
http://dx.doi.org/10.1007/BF00293232 http://hdl.handle.net/11449/223899 |
identifier_str_mv |
Chromosoma, v. 72, n. 2, p. 157-173, 1979. 0009-5915 1432-0886 10.1007/BF00293232 2-s2.0-0018296012 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Chromosoma |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
157-173 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128386664497152 |