Effects of zoledronic acid on odontoblast-like cells

• Autor(a) principal: Basso, F. G.
• Data de Publicação: 2013
• Outros Autores: Turrioni, A. P S [UNESP], Hebling, J. [UNESP], De Souza Costa, C. A. [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1016/j.archoralbio.2012.09.016; http://hdl.handle.net/11449/75203
• topic: Bisphosphonates; Cell culture; Cytotoxicity; alkaline phosphatase; bisphosphonic acid derivative; bone density conservation agent; collagen type 1; dentin sialophosphoprotein; imidazole derivative; phosphoprotein; protein synthesis inhibitor; scleroprotein; sialoglycoprotein; succinate dehydrogenase; zoledronic acid; biosynthesis; cell culture; cell survival; dose response; drug antagonism; drug effect; gene expression regulation; human; odontoblast; Alkaline Phosphatase; Bone Density Conservation Agents; Cell Survival; Cells, Cultured; Collagen Type I; Diphosphonates; Dose-Response Relationship, Drug; Extracellular Matrix Proteins; Gene Expression Regulation, Enzymologic; Humans; Imidazoles; Odontoblasts; Phosphoproteins; Protein Synthesis Inhibitors; Sialoglycoproteins; Succinate Dehydrogenase; Bovinae

The aim of the study was to evaluate the effects of a highly potent bisphosphonate, zoledronic acid (ZOL), on cultured odontoblast-like cells MDPC-23. The cells (1.5 × 104 cells/cm2) were seeded for 48 h in wells of 24-well dished. Then, the plain culture medium (DMEM) was replaced by fresh medium without fetal bovine serum. After 24 h, ZOL (1 or 5 μM) was added to the medium and maintained in contact with the cells for 24 h. After this period, the succinic dehydrogenase (SDH) enzyme production (cell viability-MTT assay), total protein (TP) production, alkaline phosphatase (ALP) activity, and gene expression (qPCR) of collagen type I (Col-I) and ALP were evaluated. Cell morphology was assessed by SEM. Five μM ZOL caused a significant decrease in SDH production. Both ZOL concentrations caused a dose-dependent significant decrease in TP production and ALP activity. ZOL also produced discret morphological alterations in the MDPC-23 cells. Regarding gene expression, 1 μM ZOL caused a significant increase in Col-I expression. Although 5 μM ZOL did not affect Col-I expression, it caused a significant alteration in ALP expression (ANOVA and Tukey's test, p < 0.05). ZOL presented a dose-dependent cytotoxic effect on the odontoblast-like cells, suggesting that under clinical conditions the release of this drug from dentin could cause damage to the pulpo-dentin complex. © 2012 Elsevier Ltd.