Effects of zoledronic acid on odontoblast-like cells
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.archoralbio.2012.09.016 http://hdl.handle.net/11449/75203 |
Resumo: | The aim of the study was to evaluate the effects of a highly potent bisphosphonate, zoledronic acid (ZOL), on cultured odontoblast-like cells MDPC-23. The cells (1.5 × 104 cells/cm2) were seeded for 48 h in wells of 24-well dished. Then, the plain culture medium (DMEM) was replaced by fresh medium without fetal bovine serum. After 24 h, ZOL (1 or 5 μM) was added to the medium and maintained in contact with the cells for 24 h. After this period, the succinic dehydrogenase (SDH) enzyme production (cell viability-MTT assay), total protein (TP) production, alkaline phosphatase (ALP) activity, and gene expression (qPCR) of collagen type I (Col-I) and ALP were evaluated. Cell morphology was assessed by SEM. Five μM ZOL caused a significant decrease in SDH production. Both ZOL concentrations caused a dose-dependent significant decrease in TP production and ALP activity. ZOL also produced discret morphological alterations in the MDPC-23 cells. Regarding gene expression, 1 μM ZOL caused a significant increase in Col-I expression. Although 5 μM ZOL did not affect Col-I expression, it caused a significant alteration in ALP expression (ANOVA and Tukey's test, p < 0.05). ZOL presented a dose-dependent cytotoxic effect on the odontoblast-like cells, suggesting that under clinical conditions the release of this drug from dentin could cause damage to the pulpo-dentin complex. © 2012 Elsevier Ltd. |
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Effects of zoledronic acid on odontoblast-like cellsBisphosphonatesCell cultureCytotoxicityalkaline phosphatasebisphosphonic acid derivativebone density conservation agentcollagen type 1dentin sialophosphoproteinimidazole derivativephosphoproteinprotein synthesis inhibitorscleroproteinsialoglycoproteinsuccinate dehydrogenasezoledronic acidbiosynthesiscell culturecell survivaldose responsedrug antagonismdrug effectgene expression regulationhumanodontoblastAlkaline PhosphataseBone Density Conservation AgentsCell SurvivalCells, CulturedCollagen Type IDiphosphonatesDose-Response Relationship, DrugExtracellular Matrix ProteinsGene Expression Regulation, EnzymologicHumansImidazolesOdontoblastsPhosphoproteinsProtein Synthesis InhibitorsSialoglycoproteinsSuccinate DehydrogenaseBovinaeThe aim of the study was to evaluate the effects of a highly potent bisphosphonate, zoledronic acid (ZOL), on cultured odontoblast-like cells MDPC-23. The cells (1.5 × 104 cells/cm2) were seeded for 48 h in wells of 24-well dished. Then, the plain culture medium (DMEM) was replaced by fresh medium without fetal bovine serum. After 24 h, ZOL (1 or 5 μM) was added to the medium and maintained in contact with the cells for 24 h. After this period, the succinic dehydrogenase (SDH) enzyme production (cell viability-MTT assay), total protein (TP) production, alkaline phosphatase (ALP) activity, and gene expression (qPCR) of collagen type I (Col-I) and ALP were evaluated. Cell morphology was assessed by SEM. Five μM ZOL caused a significant decrease in SDH production. Both ZOL concentrations caused a dose-dependent significant decrease in TP production and ALP activity. ZOL also produced discret morphological alterations in the MDPC-23 cells. Regarding gene expression, 1 μM ZOL caused a significant increase in Col-I expression. Although 5 μM ZOL did not affect Col-I expression, it caused a significant alteration in ALP expression (ANOVA and Tukey's test, p < 0.05). ZOL presented a dose-dependent cytotoxic effect on the odontoblast-like cells, suggesting that under clinical conditions the release of this drug from dentin could cause damage to the pulpo-dentin complex. © 2012 Elsevier Ltd.UNICAMP-Universidade de Campinas Piracicaba School of Dentistry, Piracicaba, SP, 13414-903UNESP-University Estadual Paulista Araraquara School of Dentistry, Araraquara, SP, 14801-903UNESP-University Estadual Paulista Araraquara School of Dentistry, Araraquara, SP, 14801-903Universidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (Unesp)Basso, F. G.Turrioni, A. P S [UNESP]Hebling, J. [UNESP]De Souza Costa, C. A. [UNESP]2014-05-27T11:29:01Z2014-05-27T11:29:01Z2013-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article467-473application/pdfhttp://dx.doi.org/10.1016/j.archoralbio.2012.09.016Archives of Oral Biology, v. 58, n. 5, p. 467-473, 2013.0003-9969http://hdl.handle.net/11449/7520310.1016/j.archoralbio.2012.09.016WOS:0003183220000042-s2.0-848760635502-s2.0-84876063550.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Oral Biology2.0500,752info:eu-repo/semantics/openAccess2023-12-10T06:17:01Zoai:repositorio.unesp.br:11449/75203Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:54:37.093461Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effects of zoledronic acid on odontoblast-like cells |
title |
Effects of zoledronic acid on odontoblast-like cells |
spellingShingle |
Effects of zoledronic acid on odontoblast-like cells Basso, F. G. Bisphosphonates Cell culture Cytotoxicity alkaline phosphatase bisphosphonic acid derivative bone density conservation agent collagen type 1 dentin sialophosphoprotein imidazole derivative phosphoprotein protein synthesis inhibitor scleroprotein sialoglycoprotein succinate dehydrogenase zoledronic acid biosynthesis cell culture cell survival dose response drug antagonism drug effect gene expression regulation human odontoblast Alkaline Phosphatase Bone Density Conservation Agents Cell Survival Cells, Cultured Collagen Type I Diphosphonates Dose-Response Relationship, Drug Extracellular Matrix Proteins Gene Expression Regulation, Enzymologic Humans Imidazoles Odontoblasts Phosphoproteins Protein Synthesis Inhibitors Sialoglycoproteins Succinate Dehydrogenase Bovinae |
title_short |
Effects of zoledronic acid on odontoblast-like cells |
title_full |
Effects of zoledronic acid on odontoblast-like cells |
title_fullStr |
Effects of zoledronic acid on odontoblast-like cells |
title_full_unstemmed |
Effects of zoledronic acid on odontoblast-like cells |
title_sort |
Effects of zoledronic acid on odontoblast-like cells |
author |
Basso, F. G. |
author_facet |
Basso, F. G. Turrioni, A. P S [UNESP] Hebling, J. [UNESP] De Souza Costa, C. A. [UNESP] |
author_role |
author |
author2 |
Turrioni, A. P S [UNESP] Hebling, J. [UNESP] De Souza Costa, C. A. [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Basso, F. G. Turrioni, A. P S [UNESP] Hebling, J. [UNESP] De Souza Costa, C. A. [UNESP] |
dc.subject.por.fl_str_mv |
Bisphosphonates Cell culture Cytotoxicity alkaline phosphatase bisphosphonic acid derivative bone density conservation agent collagen type 1 dentin sialophosphoprotein imidazole derivative phosphoprotein protein synthesis inhibitor scleroprotein sialoglycoprotein succinate dehydrogenase zoledronic acid biosynthesis cell culture cell survival dose response drug antagonism drug effect gene expression regulation human odontoblast Alkaline Phosphatase Bone Density Conservation Agents Cell Survival Cells, Cultured Collagen Type I Diphosphonates Dose-Response Relationship, Drug Extracellular Matrix Proteins Gene Expression Regulation, Enzymologic Humans Imidazoles Odontoblasts Phosphoproteins Protein Synthesis Inhibitors Sialoglycoproteins Succinate Dehydrogenase Bovinae |
topic |
Bisphosphonates Cell culture Cytotoxicity alkaline phosphatase bisphosphonic acid derivative bone density conservation agent collagen type 1 dentin sialophosphoprotein imidazole derivative phosphoprotein protein synthesis inhibitor scleroprotein sialoglycoprotein succinate dehydrogenase zoledronic acid biosynthesis cell culture cell survival dose response drug antagonism drug effect gene expression regulation human odontoblast Alkaline Phosphatase Bone Density Conservation Agents Cell Survival Cells, Cultured Collagen Type I Diphosphonates Dose-Response Relationship, Drug Extracellular Matrix Proteins Gene Expression Regulation, Enzymologic Humans Imidazoles Odontoblasts Phosphoproteins Protein Synthesis Inhibitors Sialoglycoproteins Succinate Dehydrogenase Bovinae |
description |
The aim of the study was to evaluate the effects of a highly potent bisphosphonate, zoledronic acid (ZOL), on cultured odontoblast-like cells MDPC-23. The cells (1.5 × 104 cells/cm2) were seeded for 48 h in wells of 24-well dished. Then, the plain culture medium (DMEM) was replaced by fresh medium without fetal bovine serum. After 24 h, ZOL (1 or 5 μM) was added to the medium and maintained in contact with the cells for 24 h. After this period, the succinic dehydrogenase (SDH) enzyme production (cell viability-MTT assay), total protein (TP) production, alkaline phosphatase (ALP) activity, and gene expression (qPCR) of collagen type I (Col-I) and ALP were evaluated. Cell morphology was assessed by SEM. Five μM ZOL caused a significant decrease in SDH production. Both ZOL concentrations caused a dose-dependent significant decrease in TP production and ALP activity. ZOL also produced discret morphological alterations in the MDPC-23 cells. Regarding gene expression, 1 μM ZOL caused a significant increase in Col-I expression. Although 5 μM ZOL did not affect Col-I expression, it caused a significant alteration in ALP expression (ANOVA and Tukey's test, p < 0.05). ZOL presented a dose-dependent cytotoxic effect on the odontoblast-like cells, suggesting that under clinical conditions the release of this drug from dentin could cause damage to the pulpo-dentin complex. © 2012 Elsevier Ltd. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-05-01 2014-05-27T11:29:01Z 2014-05-27T11:29:01Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.archoralbio.2012.09.016 Archives of Oral Biology, v. 58, n. 5, p. 467-473, 2013. 0003-9969 http://hdl.handle.net/11449/75203 10.1016/j.archoralbio.2012.09.016 WOS:000318322000004 2-s2.0-84876063550 2-s2.0-84876063550.pdf |
url |
http://dx.doi.org/10.1016/j.archoralbio.2012.09.016 http://hdl.handle.net/11449/75203 |
identifier_str_mv |
Archives of Oral Biology, v. 58, n. 5, p. 467-473, 2013. 0003-9969 10.1016/j.archoralbio.2012.09.016 WOS:000318322000004 2-s2.0-84876063550 2-s2.0-84876063550.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Archives of Oral Biology 2.050 0,752 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
467-473 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129138440011776 |