Alterations to oxidative stress markers in dogs after a short-term stress during transport
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1017/jns.2014.47 http://hdl.handle.net/11449/131036 |
Resumo: | While methods to evaluate antioxidant capacity in animals exist, one problem with the models is induction of oxidative stress. It is necessary to promote a great enough challenge to induce measurable alterations to oxidative parameters while ensuring the protocol is compatible with animal welfare. The aim of the present study was to evaluate caged transport as a viable short-term stress that would significantly affect oxidative parameters. Twenty adult Beagle dogs, maintained on the same diet for 60 d prior to the transport, were included in the study. To simulate the stress, the dogs were housed in pairs in transport cages (1·0 m × 1·0 m × 1·5 m), placed on a truck coupled to a trailer and transported for a period of 15 min. Blood collection was performed immediately before and again 3 h after the transportation to evaluate oxidative parameters in blood serum, including thiobarbituric acid reactive substances (TBARS), total antioxidant capacity (TAC), sequestration activity of the radical 2,2-diphenyl-1-picryl-hydrazyl (DPPH•), protein carbonylation (PC), total sulfhydryl groups (SH), alpha-tocopherol (αToc) and retinol (Ret). PC, SH and αToc were not significantly changed in the study; however, TBARS, TAC and DPPH increased, whereas Ret decreased after the transport. Although the lack of a control group of dogs not submitted to transport is a limitation to be considered, we conclude that the transport model is effective in inducing an antioxidant response in dogs and relevant blood parameters show sensitivity to this proposed model. |
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Alterations to oxidative stress markers in dogs after a short-term stress during transportAlpha-tocopherolAntioxidantsCanine nutritionDpph, 2,2-diphenyl-1-picryl-hydrazylLipoperoxidationPc, protein carbonilationRos, reactive oxygen speciesRet, retinolSh, total sulfhydryl groupsTac, total antioxidant capacityTbars, thiobarbituric acid reactive substancesThiobarbituric acidΑtoc, α-tocopherolWhile methods to evaluate antioxidant capacity in animals exist, one problem with the models is induction of oxidative stress. It is necessary to promote a great enough challenge to induce measurable alterations to oxidative parameters while ensuring the protocol is compatible with animal welfare. The aim of the present study was to evaluate caged transport as a viable short-term stress that would significantly affect oxidative parameters. Twenty adult Beagle dogs, maintained on the same diet for 60 d prior to the transport, were included in the study. To simulate the stress, the dogs were housed in pairs in transport cages (1·0 m × 1·0 m × 1·5 m), placed on a truck coupled to a trailer and transported for a period of 15 min. Blood collection was performed immediately before and again 3 h after the transportation to evaluate oxidative parameters in blood serum, including thiobarbituric acid reactive substances (TBARS), total antioxidant capacity (TAC), sequestration activity of the radical 2,2-diphenyl-1-picryl-hydrazyl (DPPH•), protein carbonylation (PC), total sulfhydryl groups (SH), alpha-tocopherol (αToc) and retinol (Ret). PC, SH and αToc were not significantly changed in the study; however, TBARS, TAC and DPPH increased, whereas Ret decreased after the transport. Although the lack of a control group of dogs not submitted to transport is a limitation to be considered, we conclude that the transport model is effective in inducing an antioxidant response in dogs and relevant blood parameters show sensitivity to this proposed model.College of Agrarian and Veterinarian Sciences (FCAV), São Paulo State University (UNESP) , Via de Acesso Professor Paulo Donato Castellane , s/n Jaboticabal , 14.884-900 SP , Brazil.Department of Animal Science (DZO) , State University of Maringá (UEM) , Av. Colombo, 5790 , Maringá 87020-900 , PR , Brazil.College of Agrarian and Veterinarian Sciences (FCAV), São Paulo State University (UNESP) , Via de Acesso Professor Paulo Donato Castellane , s/n Jaboticabal , 14.884-900 SP , Brazil.Journal of Nutritional ScienceUniversidade Estadual Paulista (Unesp)Universidade Estadual de Maringá (UEM)Ferreira, Chayanne S. [UNESP]Vasconcellos, Ricardo S.Pedreira, Raquel S. [UNESP]Silva, Flavio L. [UNESP]Sá, Fabiano C. [UNESP]Kroll, Fernanda S A [UNESP]Maria, Ana P J [UNESP]Venturini, Katiani S. [UNESP]Carciofi, Aulus C. [UNESP]2015-12-07T15:31:03Z2015-12-07T15:31:03Z2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article27application/pdfhttp://dx.doi.org/10.1017/jns.2014.47Journal Of Nutritional Science, v. 3, p. 27, 2014.2048-6790http://hdl.handle.net/11449/13103610.1017/jns.2014.47PMC4473139.pdf26101596PMC4473139PubMedreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Nutritional Science0,984info:eu-repo/semantics/openAccess2024-06-06T14:11:13Zoai:repositorio.unesp.br:11449/131036Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-06-06T14:11:13Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Alterations to oxidative stress markers in dogs after a short-term stress during transport |
title |
Alterations to oxidative stress markers in dogs after a short-term stress during transport |
spellingShingle |
Alterations to oxidative stress markers in dogs after a short-term stress during transport Ferreira, Chayanne S. [UNESP] Alpha-tocopherol Antioxidants Canine nutrition Dpph, 2,2-diphenyl-1-picryl-hydrazyl Lipoperoxidation Pc, protein carbonilation Ros, reactive oxygen species Ret, retinol Sh, total sulfhydryl groups Tac, total antioxidant capacity Tbars, thiobarbituric acid reactive substances Thiobarbituric acid Αtoc, α-tocopherol |
title_short |
Alterations to oxidative stress markers in dogs after a short-term stress during transport |
title_full |
Alterations to oxidative stress markers in dogs after a short-term stress during transport |
title_fullStr |
Alterations to oxidative stress markers in dogs after a short-term stress during transport |
title_full_unstemmed |
Alterations to oxidative stress markers in dogs after a short-term stress during transport |
title_sort |
Alterations to oxidative stress markers in dogs after a short-term stress during transport |
author |
Ferreira, Chayanne S. [UNESP] |
author_facet |
Ferreira, Chayanne S. [UNESP] Vasconcellos, Ricardo S. Pedreira, Raquel S. [UNESP] Silva, Flavio L. [UNESP] Sá, Fabiano C. [UNESP] Kroll, Fernanda S A [UNESP] Maria, Ana P J [UNESP] Venturini, Katiani S. [UNESP] Carciofi, Aulus C. [UNESP] |
author_role |
author |
author2 |
Vasconcellos, Ricardo S. Pedreira, Raquel S. [UNESP] Silva, Flavio L. [UNESP] Sá, Fabiano C. [UNESP] Kroll, Fernanda S A [UNESP] Maria, Ana P J [UNESP] Venturini, Katiani S. [UNESP] Carciofi, Aulus C. [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Estadual de Maringá (UEM) |
dc.contributor.author.fl_str_mv |
Ferreira, Chayanne S. [UNESP] Vasconcellos, Ricardo S. Pedreira, Raquel S. [UNESP] Silva, Flavio L. [UNESP] Sá, Fabiano C. [UNESP] Kroll, Fernanda S A [UNESP] Maria, Ana P J [UNESP] Venturini, Katiani S. [UNESP] Carciofi, Aulus C. [UNESP] |
dc.subject.por.fl_str_mv |
Alpha-tocopherol Antioxidants Canine nutrition Dpph, 2,2-diphenyl-1-picryl-hydrazyl Lipoperoxidation Pc, protein carbonilation Ros, reactive oxygen species Ret, retinol Sh, total sulfhydryl groups Tac, total antioxidant capacity Tbars, thiobarbituric acid reactive substances Thiobarbituric acid Αtoc, α-tocopherol |
topic |
Alpha-tocopherol Antioxidants Canine nutrition Dpph, 2,2-diphenyl-1-picryl-hydrazyl Lipoperoxidation Pc, protein carbonilation Ros, reactive oxygen species Ret, retinol Sh, total sulfhydryl groups Tac, total antioxidant capacity Tbars, thiobarbituric acid reactive substances Thiobarbituric acid Αtoc, α-tocopherol |
description |
While methods to evaluate antioxidant capacity in animals exist, one problem with the models is induction of oxidative stress. It is necessary to promote a great enough challenge to induce measurable alterations to oxidative parameters while ensuring the protocol is compatible with animal welfare. The aim of the present study was to evaluate caged transport as a viable short-term stress that would significantly affect oxidative parameters. Twenty adult Beagle dogs, maintained on the same diet for 60 d prior to the transport, were included in the study. To simulate the stress, the dogs were housed in pairs in transport cages (1·0 m × 1·0 m × 1·5 m), placed on a truck coupled to a trailer and transported for a period of 15 min. Blood collection was performed immediately before and again 3 h after the transportation to evaluate oxidative parameters in blood serum, including thiobarbituric acid reactive substances (TBARS), total antioxidant capacity (TAC), sequestration activity of the radical 2,2-diphenyl-1-picryl-hydrazyl (DPPH•), protein carbonylation (PC), total sulfhydryl groups (SH), alpha-tocopherol (αToc) and retinol (Ret). PC, SH and αToc were not significantly changed in the study; however, TBARS, TAC and DPPH increased, whereas Ret decreased after the transport. Although the lack of a control group of dogs not submitted to transport is a limitation to be considered, we conclude that the transport model is effective in inducing an antioxidant response in dogs and relevant blood parameters show sensitivity to this proposed model. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2015-12-07T15:31:03Z 2015-12-07T15:31:03Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1017/jns.2014.47 Journal Of Nutritional Science, v. 3, p. 27, 2014. 2048-6790 http://hdl.handle.net/11449/131036 10.1017/jns.2014.47 PMC4473139.pdf 26101596 PMC4473139 |
url |
http://dx.doi.org/10.1017/jns.2014.47 http://hdl.handle.net/11449/131036 |
identifier_str_mv |
Journal Of Nutritional Science, v. 3, p. 27, 2014. 2048-6790 10.1017/jns.2014.47 PMC4473139.pdf 26101596 PMC4473139 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Nutritional Science 0,984 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
27 application/pdf |
dc.publisher.none.fl_str_mv |
Journal of Nutritional Science |
publisher.none.fl_str_mv |
Journal of Nutritional Science |
dc.source.none.fl_str_mv |
PubMed reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1803045563450523648 |