Frequency of detection and phylogenetic analysis of porcine circovirus 3 (Pcv-3) in healthy primiparous and multiparous sows and their mummified fetuses and stillborn
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/pathogens9070533 http://hdl.handle.net/11449/200695 |
Resumo: | Porcine circovirus 3 (PCV-3) has been suggested as a putative causal agent of swine reproductive disease. A number of different studies have pointed out this association, but there is still a lack of information regarding the normal rates of PCV-3 infection in farms with normal reproductive parameters. The objective of the present study was to assess the frequency of PCV-3 detection in primiparous and multiparous sows and in tissues from their respective fetuses from farms with average reproductive parameters. Sera from 57 primiparous and 64 multiparous sows from 3 different farms were collected at two time points. Brain and lung tissues from 49 mummies and 206 stillborn were collected at farrowing. Samples were tested by PCR, and when positive, quantified by quantitative PCR. Thirty-nine complete genomes were obtained and phylogenetically analyzed. All sera from multiparous sows were negative, while 19/57 (33.3%) primiparous sows were PCV-3 PCR positive. From the 255 tested fetuses, 86 (33.7%) had at least one tissue positive to PCV-3. The frequency of detection in fetuses from primiparous sows (73/91, 80.2%) was significantly higher than those from multiparous ones (13/164, 7.9%). It can be concluded that PCV-3 is able to cause intrauterine infections in absence of overt reproductive disorders. |
id |
UNSP_8ea2195d24ea072093a868ec883232f5 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/200695 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Frequency of detection and phylogenetic analysis of porcine circovirus 3 (Pcv-3) in healthy primiparous and multiparous sows and their mummified fetuses and stillbornIntra-uterine infectionMummified fetusPorcine circovirus 3SowStillbornVertical transmissionPorcine circovirus 3 (PCV-3) has been suggested as a putative causal agent of swine reproductive disease. A number of different studies have pointed out this association, but there is still a lack of information regarding the normal rates of PCV-3 infection in farms with normal reproductive parameters. The objective of the present study was to assess the frequency of PCV-3 detection in primiparous and multiparous sows and in tissues from their respective fetuses from farms with average reproductive parameters. Sera from 57 primiparous and 64 multiparous sows from 3 different farms were collected at two time points. Brain and lung tissues from 49 mummies and 206 stillborn were collected at farrowing. Samples were tested by PCR, and when positive, quantified by quantitative PCR. Thirty-nine complete genomes were obtained and phylogenetically analyzed. All sera from multiparous sows were negative, while 19/57 (33.3%) primiparous sows were PCV-3 PCR positive. From the 255 tested fetuses, 86 (33.7%) had at least one tissue positive to PCV-3. The frequency of detection in fetuses from primiparous sows (73/91, 80.2%) was significantly higher than those from multiparous ones (13/164, 7.9%). It can be concluded that PCV-3 is able to cause intrauterine infections in absence of overt reproductive disorders.Centres de Recerca de CatalunyaFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Instituto Nacional de Investigación y Tecnología Agraria y AlimentariaIRTA Centre de Recerca en Sanitat Animal (CReSA IRTA-UAB) Campus de la Universitat Autònoma de Barcelona, BellaterraOIE Collaborating Centre for the Research and Control of Emerging and Re-emerging Swine Diseases in Europe (IRTA-CReSA), BellaterraZoetis Manufacturing & Research Spain S.L., Ctra. Camprodon s/n, La RibaDepartment of Immunology and Microbiology Institute of Biosciences São Paulo State University (Unesp)Departament de Sanitat i Anatomia Animals Universitat Autònoma de Barcelona (UAB)UAB Centre de Recerca en Sanitat Animal (CReSA IRTA-UAB) Campus de la Universitat Autònoma de Barcelona, BellaterraDepartment of Immunology and Microbiology Institute of Biosciences São Paulo State University (Unesp)FAPESP: 2017/26649-4Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria: E-RTA2017-00007-00-00IRTA-UAB)OIE Collaborating Centre for the Research and Control of Emerging and Re-emerging Swine Diseases in Europe (IRTA-CReSA)Zoetis Manufacturing & Research Spain S.L.Universidade Estadual Paulista (Unesp)Universitat Autònoma de Barcelona (UAB)Saporiti, VivianeMartorell, SusannaCruz, Taís F. [UNESP]Klaumann, FranciniCorrea-Fiz, FlorenciaBalasch, MònicaSibila, MarinaSegalés, Joaquim2020-12-12T02:13:37Z2020-12-12T02:13:37Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-12http://dx.doi.org/10.3390/pathogens9070533Pathogens, v. 9, n. 7, p. 1-12, 2020.2076-0817http://hdl.handle.net/11449/20069510.3390/pathogens90705332-s2.0-85087380899Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPathogensinfo:eu-repo/semantics/openAccess2021-10-23T14:26:54Zoai:repositorio.unesp.br:11449/200695Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:06:56.961031Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Frequency of detection and phylogenetic analysis of porcine circovirus 3 (Pcv-3) in healthy primiparous and multiparous sows and their mummified fetuses and stillborn |
title |
Frequency of detection and phylogenetic analysis of porcine circovirus 3 (Pcv-3) in healthy primiparous and multiparous sows and their mummified fetuses and stillborn |
spellingShingle |
Frequency of detection and phylogenetic analysis of porcine circovirus 3 (Pcv-3) in healthy primiparous and multiparous sows and their mummified fetuses and stillborn Saporiti, Viviane Intra-uterine infection Mummified fetus Porcine circovirus 3 Sow Stillborn Vertical transmission |
title_short |
Frequency of detection and phylogenetic analysis of porcine circovirus 3 (Pcv-3) in healthy primiparous and multiparous sows and their mummified fetuses and stillborn |
title_full |
Frequency of detection and phylogenetic analysis of porcine circovirus 3 (Pcv-3) in healthy primiparous and multiparous sows and their mummified fetuses and stillborn |
title_fullStr |
Frequency of detection and phylogenetic analysis of porcine circovirus 3 (Pcv-3) in healthy primiparous and multiparous sows and their mummified fetuses and stillborn |
title_full_unstemmed |
Frequency of detection and phylogenetic analysis of porcine circovirus 3 (Pcv-3) in healthy primiparous and multiparous sows and their mummified fetuses and stillborn |
title_sort |
Frequency of detection and phylogenetic analysis of porcine circovirus 3 (Pcv-3) in healthy primiparous and multiparous sows and their mummified fetuses and stillborn |
author |
Saporiti, Viviane |
author_facet |
Saporiti, Viviane Martorell, Susanna Cruz, Taís F. [UNESP] Klaumann, Francini Correa-Fiz, Florencia Balasch, Mònica Sibila, Marina Segalés, Joaquim |
author_role |
author |
author2 |
Martorell, Susanna Cruz, Taís F. [UNESP] Klaumann, Francini Correa-Fiz, Florencia Balasch, Mònica Sibila, Marina Segalés, Joaquim |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
IRTA-UAB) OIE Collaborating Centre for the Research and Control of Emerging and Re-emerging Swine Diseases in Europe (IRTA-CReSA) Zoetis Manufacturing & Research Spain S.L. Universidade Estadual Paulista (Unesp) Universitat Autònoma de Barcelona (UAB) |
dc.contributor.author.fl_str_mv |
Saporiti, Viviane Martorell, Susanna Cruz, Taís F. [UNESP] Klaumann, Francini Correa-Fiz, Florencia Balasch, Mònica Sibila, Marina Segalés, Joaquim |
dc.subject.por.fl_str_mv |
Intra-uterine infection Mummified fetus Porcine circovirus 3 Sow Stillborn Vertical transmission |
topic |
Intra-uterine infection Mummified fetus Porcine circovirus 3 Sow Stillborn Vertical transmission |
description |
Porcine circovirus 3 (PCV-3) has been suggested as a putative causal agent of swine reproductive disease. A number of different studies have pointed out this association, but there is still a lack of information regarding the normal rates of PCV-3 infection in farms with normal reproductive parameters. The objective of the present study was to assess the frequency of PCV-3 detection in primiparous and multiparous sows and in tissues from their respective fetuses from farms with average reproductive parameters. Sera from 57 primiparous and 64 multiparous sows from 3 different farms were collected at two time points. Brain and lung tissues from 49 mummies and 206 stillborn were collected at farrowing. Samples were tested by PCR, and when positive, quantified by quantitative PCR. Thirty-nine complete genomes were obtained and phylogenetically analyzed. All sera from multiparous sows were negative, while 19/57 (33.3%) primiparous sows were PCV-3 PCR positive. From the 255 tested fetuses, 86 (33.7%) had at least one tissue positive to PCV-3. The frequency of detection in fetuses from primiparous sows (73/91, 80.2%) was significantly higher than those from multiparous ones (13/164, 7.9%). It can be concluded that PCV-3 is able to cause intrauterine infections in absence of overt reproductive disorders. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:13:37Z 2020-12-12T02:13:37Z 2020-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/pathogens9070533 Pathogens, v. 9, n. 7, p. 1-12, 2020. 2076-0817 http://hdl.handle.net/11449/200695 10.3390/pathogens9070533 2-s2.0-85087380899 |
url |
http://dx.doi.org/10.3390/pathogens9070533 http://hdl.handle.net/11449/200695 |
identifier_str_mv |
Pathogens, v. 9, n. 7, p. 1-12, 2020. 2076-0817 10.3390/pathogens9070533 2-s2.0-85087380899 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pathogens |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-12 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129585526603776 |