The role of triiodothyronine hormone and mechanically-stressed endothelial cell paracrine signalling synergism in gene reprogramming during hBMSC-stimulated osteogenic phenotype in vitro
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.mce.2018.08.008 http://hdl.handle.net/11449/188105 |
Resumo: | We therefore investigated whether there is synergism between triiodothyronine (T3) hormone and trophic molecules released from mechanically-stressed endothelial cells (EC-enriched medium) in osteogenic phenotype by mapping classical repertory of genes. Although there are studies reporting the efficiency of T3 hormone on bone cells, it is scarce considering their effect in conjunction with other physiologically active molecules, such as those released by the active endothelial cells. To address this issue, human bone marrow-derived mesenchymal stem cells (hBMSCs) were treated with EC-enriched medium subjected to shear-stress up to 72 h in vitro, in conjunction or not with T3 hormone. Although our results found an important synergism considering these parameters on modulating key bone-related gene markers, such as on the alkaline phosphatase (ALP) behavior (at both mRNA and protein content), contributing for osteoblast differentiation, important genes such as OSTERIX and RUNX2 were significantly down-expressed, while a over-expression of RANKL was found when the conjunction effect of T3 and endothelial paracrine signaling was considered. In addition, T3 hormone over expressed both OCT4 and NANOG genes in a DNA epigenetic-independent manner. However, we observed a dynamic reprogramming of DNMT1, DNMT3A, DNMT3B and TET1, important DNA-related epigenetic markers. Specifically, T3 hormone alone up-modulated TET2 transcripts profile. Complimentarily, expression of microRNA (miRs) processing-related genes also was modulated, as well as miR-10b, miR-22, miR-21, miR-143 and miR-145 transcriptional related profiles. Altogether, our results suggested a positive effect of mechanically-stressed endothelial cells-induced paracrine signaling on T3 hormone-obtaining osteogenic phenotype, contributing to understanding the paradoxal effect of T3 hormone on the bone physiology. |
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The role of triiodothyronine hormone and mechanically-stressed endothelial cell paracrine signalling synergism in gene reprogramming during hBMSC-stimulated osteogenic phenotype in vitroBoneEpigeneticMesenchymal stem cellsOsteoblastOsteogenic phenotypeThyroidTriiodothyronineWe therefore investigated whether there is synergism between triiodothyronine (T3) hormone and trophic molecules released from mechanically-stressed endothelial cells (EC-enriched medium) in osteogenic phenotype by mapping classical repertory of genes. Although there are studies reporting the efficiency of T3 hormone on bone cells, it is scarce considering their effect in conjunction with other physiologically active molecules, such as those released by the active endothelial cells. To address this issue, human bone marrow-derived mesenchymal stem cells (hBMSCs) were treated with EC-enriched medium subjected to shear-stress up to 72 h in vitro, in conjunction or not with T3 hormone. Although our results found an important synergism considering these parameters on modulating key bone-related gene markers, such as on the alkaline phosphatase (ALP) behavior (at both mRNA and protein content), contributing for osteoblast differentiation, important genes such as OSTERIX and RUNX2 were significantly down-expressed, while a over-expression of RANKL was found when the conjunction effect of T3 and endothelial paracrine signaling was considered. In addition, T3 hormone over expressed both OCT4 and NANOG genes in a DNA epigenetic-independent manner. However, we observed a dynamic reprogramming of DNMT1, DNMT3A, DNMT3B and TET1, important DNA-related epigenetic markers. Specifically, T3 hormone alone up-modulated TET2 transcripts profile. Complimentarily, expression of microRNA (miRs) processing-related genes also was modulated, as well as miR-10b, miR-22, miR-21, miR-143 and miR-145 transcriptional related profiles. Altogether, our results suggested a positive effect of mechanically-stressed endothelial cells-induced paracrine signaling on T3 hormone-obtaining osteogenic phenotype, contributing to understanding the paradoxal effect of T3 hormone on the bone physiology.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Chemistry and Biochemistry São Paulo State University (UNESP) Institute of Biosciences, Campus BotucatuElectron Microscopy Center São Paulo State University (UNESP) Institute of Biosciences, Campus BotucatuÁrea de Periodontia Departamento de Prótese e Periodontia Faculdade de Odontologia de Piracicaba Universidade de CampinasÁrea de Epigenética Faculdade de Odontologia Universidade PaulistaDepartment of Chemistry and Biochemistry São Paulo State University (UNESP) Institute of Biosciences, Campus BotucatuElectron Microscopy Center São Paulo State University (UNESP) Institute of Biosciences, Campus BotucatuFAPESP: 2014/22689-3FAPESP: 2016/01139-0FAPESP: 2016/10392-1Universidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Universidade Paulistada Silva, Rodrigo A. [UNESP]de Camargo Andrade, Amanda Fantini [UNESP]da Silva Feltran, Geórgia [UNESP]Fernandes, Célio Júnior da C. [UNESP]de Assis, Rahyza Inacio F.Ferreira, Marcel Rodrigues [UNESP]Andia, Denise C.Zambuzzi, Willian F. [UNESP]2019-10-06T15:57:24Z2019-10-06T15:57:24Z2018-12-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article151-167http://dx.doi.org/10.1016/j.mce.2018.08.008Molecular and Cellular Endocrinology, v. 478, p. 151-167.1872-80570303-7207http://hdl.handle.net/11449/18810510.1016/j.mce.2018.08.0082-s2.0-85053845440Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMolecular and Cellular Endocrinologyinfo:eu-repo/semantics/openAccess2021-10-23T19:28:01Zoai:repositorio.unesp.br:11449/188105Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T19:28:01Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The role of triiodothyronine hormone and mechanically-stressed endothelial cell paracrine signalling synergism in gene reprogramming during hBMSC-stimulated osteogenic phenotype in vitro |
title |
The role of triiodothyronine hormone and mechanically-stressed endothelial cell paracrine signalling synergism in gene reprogramming during hBMSC-stimulated osteogenic phenotype in vitro |
spellingShingle |
The role of triiodothyronine hormone and mechanically-stressed endothelial cell paracrine signalling synergism in gene reprogramming during hBMSC-stimulated osteogenic phenotype in vitro da Silva, Rodrigo A. [UNESP] Bone Epigenetic Mesenchymal stem cells Osteoblast Osteogenic phenotype Thyroid Triiodothyronine |
title_short |
The role of triiodothyronine hormone and mechanically-stressed endothelial cell paracrine signalling synergism in gene reprogramming during hBMSC-stimulated osteogenic phenotype in vitro |
title_full |
The role of triiodothyronine hormone and mechanically-stressed endothelial cell paracrine signalling synergism in gene reprogramming during hBMSC-stimulated osteogenic phenotype in vitro |
title_fullStr |
The role of triiodothyronine hormone and mechanically-stressed endothelial cell paracrine signalling synergism in gene reprogramming during hBMSC-stimulated osteogenic phenotype in vitro |
title_full_unstemmed |
The role of triiodothyronine hormone and mechanically-stressed endothelial cell paracrine signalling synergism in gene reprogramming during hBMSC-stimulated osteogenic phenotype in vitro |
title_sort |
The role of triiodothyronine hormone and mechanically-stressed endothelial cell paracrine signalling synergism in gene reprogramming during hBMSC-stimulated osteogenic phenotype in vitro |
author |
da Silva, Rodrigo A. [UNESP] |
author_facet |
da Silva, Rodrigo A. [UNESP] de Camargo Andrade, Amanda Fantini [UNESP] da Silva Feltran, Geórgia [UNESP] Fernandes, Célio Júnior da C. [UNESP] de Assis, Rahyza Inacio F. Ferreira, Marcel Rodrigues [UNESP] Andia, Denise C. Zambuzzi, Willian F. [UNESP] |
author_role |
author |
author2 |
de Camargo Andrade, Amanda Fantini [UNESP] da Silva Feltran, Geórgia [UNESP] Fernandes, Célio Júnior da C. [UNESP] de Assis, Rahyza Inacio F. Ferreira, Marcel Rodrigues [UNESP] Andia, Denise C. Zambuzzi, Willian F. [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Estadual de Campinas (UNICAMP) Universidade Paulista |
dc.contributor.author.fl_str_mv |
da Silva, Rodrigo A. [UNESP] de Camargo Andrade, Amanda Fantini [UNESP] da Silva Feltran, Geórgia [UNESP] Fernandes, Célio Júnior da C. [UNESP] de Assis, Rahyza Inacio F. Ferreira, Marcel Rodrigues [UNESP] Andia, Denise C. Zambuzzi, Willian F. [UNESP] |
dc.subject.por.fl_str_mv |
Bone Epigenetic Mesenchymal stem cells Osteoblast Osteogenic phenotype Thyroid Triiodothyronine |
topic |
Bone Epigenetic Mesenchymal stem cells Osteoblast Osteogenic phenotype Thyroid Triiodothyronine |
description |
We therefore investigated whether there is synergism between triiodothyronine (T3) hormone and trophic molecules released from mechanically-stressed endothelial cells (EC-enriched medium) in osteogenic phenotype by mapping classical repertory of genes. Although there are studies reporting the efficiency of T3 hormone on bone cells, it is scarce considering their effect in conjunction with other physiologically active molecules, such as those released by the active endothelial cells. To address this issue, human bone marrow-derived mesenchymal stem cells (hBMSCs) were treated with EC-enriched medium subjected to shear-stress up to 72 h in vitro, in conjunction or not with T3 hormone. Although our results found an important synergism considering these parameters on modulating key bone-related gene markers, such as on the alkaline phosphatase (ALP) behavior (at both mRNA and protein content), contributing for osteoblast differentiation, important genes such as OSTERIX and RUNX2 were significantly down-expressed, while a over-expression of RANKL was found when the conjunction effect of T3 and endothelial paracrine signaling was considered. In addition, T3 hormone over expressed both OCT4 and NANOG genes in a DNA epigenetic-independent manner. However, we observed a dynamic reprogramming of DNMT1, DNMT3A, DNMT3B and TET1, important DNA-related epigenetic markers. Specifically, T3 hormone alone up-modulated TET2 transcripts profile. Complimentarily, expression of microRNA (miRs) processing-related genes also was modulated, as well as miR-10b, miR-22, miR-21, miR-143 and miR-145 transcriptional related profiles. Altogether, our results suggested a positive effect of mechanically-stressed endothelial cells-induced paracrine signaling on T3 hormone-obtaining osteogenic phenotype, contributing to understanding the paradoxal effect of T3 hormone on the bone physiology. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-15 2019-10-06T15:57:24Z 2019-10-06T15:57:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.mce.2018.08.008 Molecular and Cellular Endocrinology, v. 478, p. 151-167. 1872-8057 0303-7207 http://hdl.handle.net/11449/188105 10.1016/j.mce.2018.08.008 2-s2.0-85053845440 |
url |
http://dx.doi.org/10.1016/j.mce.2018.08.008 http://hdl.handle.net/11449/188105 |
identifier_str_mv |
Molecular and Cellular Endocrinology, v. 478, p. 151-167. 1872-8057 0303-7207 10.1016/j.mce.2018.08.008 2-s2.0-85053845440 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Molecular and Cellular Endocrinology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
151-167 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803046252329304064 |