Inactivation of Streptococcus mutans genes lytST and dltAD impairs its pathogenicity in vivo
Main Author: | |
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Publication Date: | 2019 |
Other Authors: | , , , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UNESP |
Download full: | http://dx.doi.org/10.1080/20002297.2019.1607505 http://hdl.handle.net/11449/187666 |
Summary: | Background: Streptococcus mutans orchestrates the development of a biofilm that causes dental caries in the presence of dietary sucrose, and, in the bloodstream, S. mutans can cause systemic infections. The development of a cariogenic biofilm is dependent on the formation of an extracellular matrix rich in exopolysaccharides, which contains extracellular DNA (eDNA) and lipoteichoic acids (LTAs). While the exopolysaccharides are virulence markers, the involvement of genes linked to eDNA and LTAs metabolism in the pathogenicity of S. mutans remains unclear. Objective and Design: In this study, a parental strain S. mutans UA159 and derivative strains carrying single gene deletions were used to investigate the role of eDNA (ΔlytS and ΔlytT), LTA (ΔdltA and ΔdltD), and insoluble exopolysaccharides (ΔgtfB) in virulence in a rodent model of dental caries (rats) and a systemic infection model (Galleria mellonella larvae). Results: Fewer carious lesions were observed on smooth and sulcal surfaces of enamel and dentin of the rats infected with ∆lytS, ∆dltD, and ΔgtfB (vs. the parental strain). Moreover, strains carrying gene deletions prevented the killing of larvae (vs. the parental strain). Conclusions: Altogether, these findings indicate that inactivation of lytST and dltAD impaired S. mutans cariogenicity and virulence in vivo. |
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Inactivation of Streptococcus mutans genes lytST and dltAD impairs its pathogenicity in vivodental carieseDNAExopolysaccharideslipoteichoic acidsoxidative stresssystemic infectionBackground: Streptococcus mutans orchestrates the development of a biofilm that causes dental caries in the presence of dietary sucrose, and, in the bloodstream, S. mutans can cause systemic infections. The development of a cariogenic biofilm is dependent on the formation of an extracellular matrix rich in exopolysaccharides, which contains extracellular DNA (eDNA) and lipoteichoic acids (LTAs). While the exopolysaccharides are virulence markers, the involvement of genes linked to eDNA and LTAs metabolism in the pathogenicity of S. mutans remains unclear. Objective and Design: In this study, a parental strain S. mutans UA159 and derivative strains carrying single gene deletions were used to investigate the role of eDNA (ΔlytS and ΔlytT), LTA (ΔdltA and ΔdltD), and insoluble exopolysaccharides (ΔgtfB) in virulence in a rodent model of dental caries (rats) and a systemic infection model (Galleria mellonella larvae). Results: Fewer carious lesions were observed on smooth and sulcal surfaces of enamel and dentin of the rats infected with ∆lytS, ∆dltD, and ΔgtfB (vs. the parental strain). Moreover, strains carrying gene deletions prevented the killing of larvae (vs. the parental strain). Conclusions: Altogether, these findings indicate that inactivation of lytST and dltAD impaired S. mutans cariogenicity and virulence in vivo.Department of Dental Materials and Prosthodontics Sao Paulo State University (Unesp) School of DentistryDepartment of Physiological Sciences Piracicaba Dental School University of Campinas–UNICAMPDepartment of Pediatric Dentistry Piracicaba Dental School University of Campinas–UNICAMPCenter for Oral Biology University of RochesterDepartment of Dental Materials and Prosthodontics Sao Paulo State University (Unesp) School of DentistryUniversidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)University of RochesterCastillo Pedraza, Midian C. [UNESP]Rosalen, Pedro L.Castilho, Aline Rogéria Freire deFreires, Irlan de Almeidade Sales Leite, Luana [UNESP]Faustoferri, Roberta C.Quivey Jr, Robert G.Klein, Marlise I. [UNESP]2019-10-06T15:43:30Z2019-10-06T15:43:30Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1080/20002297.2019.1607505Journal of Oral Microbiology, v. 11, n. 1, 2019.2000-2297http://hdl.handle.net/11449/18766610.1080/20002297.2019.16075052-s2.0-85065714274Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Oral Microbiologyinfo:eu-repo/semantics/openAccess2021-10-23T17:52:04Zoai:repositorio.unesp.br:11449/187666Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T17:52:04Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Inactivation of Streptococcus mutans genes lytST and dltAD impairs its pathogenicity in vivo |
title |
Inactivation of Streptococcus mutans genes lytST and dltAD impairs its pathogenicity in vivo |
spellingShingle |
Inactivation of Streptococcus mutans genes lytST and dltAD impairs its pathogenicity in vivo Castillo Pedraza, Midian C. [UNESP] dental caries eDNA Exopolysaccharides lipoteichoic acids oxidative stress systemic infection |
title_short |
Inactivation of Streptococcus mutans genes lytST and dltAD impairs its pathogenicity in vivo |
title_full |
Inactivation of Streptococcus mutans genes lytST and dltAD impairs its pathogenicity in vivo |
title_fullStr |
Inactivation of Streptococcus mutans genes lytST and dltAD impairs its pathogenicity in vivo |
title_full_unstemmed |
Inactivation of Streptococcus mutans genes lytST and dltAD impairs its pathogenicity in vivo |
title_sort |
Inactivation of Streptococcus mutans genes lytST and dltAD impairs its pathogenicity in vivo |
author |
Castillo Pedraza, Midian C. [UNESP] |
author_facet |
Castillo Pedraza, Midian C. [UNESP] Rosalen, Pedro L. Castilho, Aline Rogéria Freire de Freires, Irlan de Almeida de Sales Leite, Luana [UNESP] Faustoferri, Roberta C. Quivey Jr, Robert G. Klein, Marlise I. [UNESP] |
author_role |
author |
author2 |
Rosalen, Pedro L. Castilho, Aline Rogéria Freire de Freires, Irlan de Almeida de Sales Leite, Luana [UNESP] Faustoferri, Roberta C. Quivey Jr, Robert G. Klein, Marlise I. [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Estadual de Campinas (UNICAMP) University of Rochester |
dc.contributor.author.fl_str_mv |
Castillo Pedraza, Midian C. [UNESP] Rosalen, Pedro L. Castilho, Aline Rogéria Freire de Freires, Irlan de Almeida de Sales Leite, Luana [UNESP] Faustoferri, Roberta C. Quivey Jr, Robert G. Klein, Marlise I. [UNESP] |
dc.subject.por.fl_str_mv |
dental caries eDNA Exopolysaccharides lipoteichoic acids oxidative stress systemic infection |
topic |
dental caries eDNA Exopolysaccharides lipoteichoic acids oxidative stress systemic infection |
description |
Background: Streptococcus mutans orchestrates the development of a biofilm that causes dental caries in the presence of dietary sucrose, and, in the bloodstream, S. mutans can cause systemic infections. The development of a cariogenic biofilm is dependent on the formation of an extracellular matrix rich in exopolysaccharides, which contains extracellular DNA (eDNA) and lipoteichoic acids (LTAs). While the exopolysaccharides are virulence markers, the involvement of genes linked to eDNA and LTAs metabolism in the pathogenicity of S. mutans remains unclear. Objective and Design: In this study, a parental strain S. mutans UA159 and derivative strains carrying single gene deletions were used to investigate the role of eDNA (ΔlytS and ΔlytT), LTA (ΔdltA and ΔdltD), and insoluble exopolysaccharides (ΔgtfB) in virulence in a rodent model of dental caries (rats) and a systemic infection model (Galleria mellonella larvae). Results: Fewer carious lesions were observed on smooth and sulcal surfaces of enamel and dentin of the rats infected with ∆lytS, ∆dltD, and ΔgtfB (vs. the parental strain). Moreover, strains carrying gene deletions prevented the killing of larvae (vs. the parental strain). Conclusions: Altogether, these findings indicate that inactivation of lytST and dltAD impaired S. mutans cariogenicity and virulence in vivo. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T15:43:30Z 2019-10-06T15:43:30Z 2019-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1080/20002297.2019.1607505 Journal of Oral Microbiology, v. 11, n. 1, 2019. 2000-2297 http://hdl.handle.net/11449/187666 10.1080/20002297.2019.1607505 2-s2.0-85065714274 |
url |
http://dx.doi.org/10.1080/20002297.2019.1607505 http://hdl.handle.net/11449/187666 |
identifier_str_mv |
Journal of Oral Microbiology, v. 11, n. 1, 2019. 2000-2297 10.1080/20002297.2019.1607505 2-s2.0-85065714274 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Oral Microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799965195129323520 |