Long-term evaluation of poly(Lactic acid) (PLA) implants in a horse: An experimental pilot study
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/molecules26237224 http://hdl.handle.net/11449/233852 |
Resumo: | In horses, there is an increasing interest in developing long-lasting drug formulations, with biopolymers as viable carrier alternatives in addition to their use as scaffolds, suture threads, screws, pins, and plates for orthopedic surgeries. This communication focuses on the prolonged biocompatibility and biodegradation of PLA, prepared by hot pressing at 180◦ C. Six samples were implanted subcutaneously on the lateral surface of the neck of one horse. The polymers remained implanted for 24 to 57 weeks. Physical examination, plasma fibrinogen, and the mechanical nociceptive threshold (MNT) were performed. After 24, 28, 34, 38, and 57 weeks, the materials were removed for histochemical analysis using hematoxylin-eosin and scanning electron microscopy (SEM). There were no essential clinical changes. MNT decreased after the implantation procedure, returning to normal after 48 h. A foreign body response was observed by histopathologic evaluation up to 38 weeks. At 57 weeks, no polymer or fibrotic capsules were identified. SEM showed surface roughness suggesting a biodegradation process, with an increase in the median pore diameter. As in the histopathological evaluation, it was not possible to detect the polymer 57 weeks after implantation. PLA showed biocompatible degradation and these findings may contribute to future research in the biomedical area. |
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Long-term evaluation of poly(Lactic acid) (PLA) implants in a horse: An experimental pilot studyBiocompatibilityBiodegradationBiomaterialPolylactide-based materialsPolymerScanning electron microscopyIn horses, there is an increasing interest in developing long-lasting drug formulations, with biopolymers as viable carrier alternatives in addition to their use as scaffolds, suture threads, screws, pins, and plates for orthopedic surgeries. This communication focuses on the prolonged biocompatibility and biodegradation of PLA, prepared by hot pressing at 180◦ C. Six samples were implanted subcutaneously on the lateral surface of the neck of one horse. The polymers remained implanted for 24 to 57 weeks. Physical examination, plasma fibrinogen, and the mechanical nociceptive threshold (MNT) were performed. After 24, 28, 34, 38, and 57 weeks, the materials were removed for histochemical analysis using hematoxylin-eosin and scanning electron microscopy (SEM). There were no essential clinical changes. MNT decreased after the implantation procedure, returning to normal after 48 h. A foreign body response was observed by histopathologic evaluation up to 38 weeks. At 57 weeks, no polymer or fibrotic capsules were identified. SEM showed surface roughness suggesting a biodegradation process, with an increase in the median pore diameter. As in the histopathological evaluation, it was not possible to detect the polymer 57 weeks after implantation. PLA showed biocompatible degradation and these findings may contribute to future research in the biomedical area.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)School of Agricultural and Veterinarian Sciences—FCAV São Paulo State University—UNESPVeterinary School Federal University of Minas Gerais—UFMGSão Carlos School of Engineering—EESC University of São Paulo—USPSchool of Agricultural and Veterinarian Sciences—FCAV São Paulo State University—UNESPCAPES: 001CNPq: 132700/2017-4FAPESP: 2015/26738-1FAPESP: 2017/10959-4FAPESP: 2018/13685-5FAPESP: 2019/16779-3Universidade Estadual Paulista (UNESP)Universidade Federal de Minas Gerais (UFMG)Universidade de São Paulo (USP)Carvalho, Júlia Ribeiro Garcia [UNESP]Conde, Gabriel [UNESP]Antonioli, Marina Lansarini [UNESP]Santana, Clarissa HelenaLittiere, Thayssa Oliveira [UNESP]Dias, Paula PatrocínioChinelatto, Marcelo AparecidoCanola, Paulo Aléscio [UNESP]Zara, Fernando José. [UNESP]Ferraz, Guilherme Camargo [UNESP]2022-05-01T11:07:19Z2022-05-01T11:07:19Z2021-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/molecules26237224Molecules, v. 26, n. 23, 2021.1420-3049http://hdl.handle.net/11449/23385210.3390/molecules262372242-s2.0-85120169901Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMoleculesinfo:eu-repo/semantics/openAccess2024-06-06T13:05:22Zoai:repositorio.unesp.br:11449/233852Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:07:56.042688Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Long-term evaluation of poly(Lactic acid) (PLA) implants in a horse: An experimental pilot study |
title |
Long-term evaluation of poly(Lactic acid) (PLA) implants in a horse: An experimental pilot study |
spellingShingle |
Long-term evaluation of poly(Lactic acid) (PLA) implants in a horse: An experimental pilot study Carvalho, Júlia Ribeiro Garcia [UNESP] Biocompatibility Biodegradation Biomaterial Polylactide-based materials Polymer Scanning electron microscopy |
title_short |
Long-term evaluation of poly(Lactic acid) (PLA) implants in a horse: An experimental pilot study |
title_full |
Long-term evaluation of poly(Lactic acid) (PLA) implants in a horse: An experimental pilot study |
title_fullStr |
Long-term evaluation of poly(Lactic acid) (PLA) implants in a horse: An experimental pilot study |
title_full_unstemmed |
Long-term evaluation of poly(Lactic acid) (PLA) implants in a horse: An experimental pilot study |
title_sort |
Long-term evaluation of poly(Lactic acid) (PLA) implants in a horse: An experimental pilot study |
author |
Carvalho, Júlia Ribeiro Garcia [UNESP] |
author_facet |
Carvalho, Júlia Ribeiro Garcia [UNESP] Conde, Gabriel [UNESP] Antonioli, Marina Lansarini [UNESP] Santana, Clarissa Helena Littiere, Thayssa Oliveira [UNESP] Dias, Paula Patrocínio Chinelatto, Marcelo Aparecido Canola, Paulo Aléscio [UNESP] Zara, Fernando José. [UNESP] Ferraz, Guilherme Camargo [UNESP] |
author_role |
author |
author2 |
Conde, Gabriel [UNESP] Antonioli, Marina Lansarini [UNESP] Santana, Clarissa Helena Littiere, Thayssa Oliveira [UNESP] Dias, Paula Patrocínio Chinelatto, Marcelo Aparecido Canola, Paulo Aléscio [UNESP] Zara, Fernando José. [UNESP] Ferraz, Guilherme Camargo [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade Federal de Minas Gerais (UFMG) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Carvalho, Júlia Ribeiro Garcia [UNESP] Conde, Gabriel [UNESP] Antonioli, Marina Lansarini [UNESP] Santana, Clarissa Helena Littiere, Thayssa Oliveira [UNESP] Dias, Paula Patrocínio Chinelatto, Marcelo Aparecido Canola, Paulo Aléscio [UNESP] Zara, Fernando José. [UNESP] Ferraz, Guilherme Camargo [UNESP] |
dc.subject.por.fl_str_mv |
Biocompatibility Biodegradation Biomaterial Polylactide-based materials Polymer Scanning electron microscopy |
topic |
Biocompatibility Biodegradation Biomaterial Polylactide-based materials Polymer Scanning electron microscopy |
description |
In horses, there is an increasing interest in developing long-lasting drug formulations, with biopolymers as viable carrier alternatives in addition to their use as scaffolds, suture threads, screws, pins, and plates for orthopedic surgeries. This communication focuses on the prolonged biocompatibility and biodegradation of PLA, prepared by hot pressing at 180◦ C. Six samples were implanted subcutaneously on the lateral surface of the neck of one horse. The polymers remained implanted for 24 to 57 weeks. Physical examination, plasma fibrinogen, and the mechanical nociceptive threshold (MNT) were performed. After 24, 28, 34, 38, and 57 weeks, the materials were removed for histochemical analysis using hematoxylin-eosin and scanning electron microscopy (SEM). There were no essential clinical changes. MNT decreased after the implantation procedure, returning to normal after 48 h. A foreign body response was observed by histopathologic evaluation up to 38 weeks. At 57 weeks, no polymer or fibrotic capsules were identified. SEM showed surface roughness suggesting a biodegradation process, with an increase in the median pore diameter. As in the histopathological evaluation, it was not possible to detect the polymer 57 weeks after implantation. PLA showed biocompatible degradation and these findings may contribute to future research in the biomedical area. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-12-01 2022-05-01T11:07:19Z 2022-05-01T11:07:19Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/molecules26237224 Molecules, v. 26, n. 23, 2021. 1420-3049 http://hdl.handle.net/11449/233852 10.3390/molecules26237224 2-s2.0-85120169901 |
url |
http://dx.doi.org/10.3390/molecules26237224 http://hdl.handle.net/11449/233852 |
identifier_str_mv |
Molecules, v. 26, n. 23, 2021. 1420-3049 10.3390/molecules26237224 2-s2.0-85120169901 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Molecules |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129492375306240 |