Recreating the Follicular Environment: A Customized Approach for In Vitro Culture of Bovine Oocytes Based on the Origin and Differentiation State

Detalhes bibliográficos
Autor(a) principal: Luciano, Alberto Maria
Data de Publicação: 2021
Outros Autores: Barros, Rodrigo Garcia, Soares, Ana Caroline Silva [UNESP], Buratini, Jose [UNESP], Lodde, Valentina, Franciosi, Federica
Tipo de documento: Capítulo de livro
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/978-1-0716-1246-0_1
http://hdl.handle.net/11449/233101
Resumo: The mammalian ovary is a large source of oocytes organized into follicles at various stages of folliculogenesis. However, only a limited number of them can be used for in vitro embryo production (IVEP), while most have yet to complete growth and development to attain full meiotic and embryonic developmental competence. While the in vitro growth of primordial follicles in the ovarian cortex has the potential to produce mature oocytes, it is still at an experimental stage. The population of early antral follicles (EAFs), instead, may represent a reserve of oocytes close to completing the growth phase, which might be more easily exploited in vitro and could increase the number of female gametes dedicated to IVEP. Here we present in vitro culture strategies that have been developed utilizing physiological parameters to support the specific needs of oocytes at distinct stages of differentiation, in order to expand the source of female gametes for IVEP by maximizing the attainment of fertilizable oocytes. Furthermore, these culture systems provide powerful tools to dissect the molecular processes that direct the final differentiation of the mammalian oocyte.
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spelling Recreating the Follicular Environment: A Customized Approach for In Vitro Culture of Bovine Oocytes Based on the Origin and Differentiation StateChromatinCumulus cellsGap junctionGerminal vesicleIn vitro oocyte growthIntercellular communicationMeiotic arrestOocyteOogenesisPrematurationThe mammalian ovary is a large source of oocytes organized into follicles at various stages of folliculogenesis. However, only a limited number of them can be used for in vitro embryo production (IVEP), while most have yet to complete growth and development to attain full meiotic and embryonic developmental competence. While the in vitro growth of primordial follicles in the ovarian cortex has the potential to produce mature oocytes, it is still at an experimental stage. The population of early antral follicles (EAFs), instead, may represent a reserve of oocytes close to completing the growth phase, which might be more easily exploited in vitro and could increase the number of female gametes dedicated to IVEP. Here we present in vitro culture strategies that have been developed utilizing physiological parameters to support the specific needs of oocytes at distinct stages of differentiation, in order to expand the source of female gametes for IVEP by maximizing the attainment of fertilizable oocytes. Furthermore, these culture systems provide powerful tools to dissect the molecular processes that direct the final differentiation of the mammalian oocyte.Reproductive and Developmental Biology Laboratory Department of Health Animal Science and Food Safety University of MilanDepartment of Structural and Functional Biology Institute of Biosciences Sao Paulo State UniversityBiogenesi Reproductive Medicine CentreDepartment of Structural and Functional Biology Institute of Biosciences Sao Paulo State UniversityUniversity of MilanUniversidade Estadual Paulista (UNESP)Reproductive Medicine CentreLuciano, Alberto MariaBarros, Rodrigo GarciaSoares, Ana Caroline Silva [UNESP]Buratini, Jose [UNESP]Lodde, ValentinaFranciosi, Federica2022-05-01T03:42:47Z2022-05-01T03:42:47Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bookPart1-15http://dx.doi.org/10.1007/978-1-0716-1246-0_1Methods in Molecular Biology, v. 2273, p. 1-15.1940-60291064-3745http://hdl.handle.net/11449/23310110.1007/978-1-0716-1246-0_12-s2.0-85101716304Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMethods in Molecular Biologyinfo:eu-repo/semantics/openAccess2022-05-01T03:42:47Zoai:repositorio.unesp.br:11449/233101Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:00:56.211873Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Recreating the Follicular Environment: A Customized Approach for In Vitro Culture of Bovine Oocytes Based on the Origin and Differentiation State
title Recreating the Follicular Environment: A Customized Approach for In Vitro Culture of Bovine Oocytes Based on the Origin and Differentiation State
spellingShingle Recreating the Follicular Environment: A Customized Approach for In Vitro Culture of Bovine Oocytes Based on the Origin and Differentiation State
Luciano, Alberto Maria
Chromatin
Cumulus cells
Gap junction
Germinal vesicle
In vitro oocyte growth
Intercellular communication
Meiotic arrest
Oocyte
Oogenesis
Prematuration
title_short Recreating the Follicular Environment: A Customized Approach for In Vitro Culture of Bovine Oocytes Based on the Origin and Differentiation State
title_full Recreating the Follicular Environment: A Customized Approach for In Vitro Culture of Bovine Oocytes Based on the Origin and Differentiation State
title_fullStr Recreating the Follicular Environment: A Customized Approach for In Vitro Culture of Bovine Oocytes Based on the Origin and Differentiation State
title_full_unstemmed Recreating the Follicular Environment: A Customized Approach for In Vitro Culture of Bovine Oocytes Based on the Origin and Differentiation State
title_sort Recreating the Follicular Environment: A Customized Approach for In Vitro Culture of Bovine Oocytes Based on the Origin and Differentiation State
author Luciano, Alberto Maria
author_facet Luciano, Alberto Maria
Barros, Rodrigo Garcia
Soares, Ana Caroline Silva [UNESP]
Buratini, Jose [UNESP]
Lodde, Valentina
Franciosi, Federica
author_role author
author2 Barros, Rodrigo Garcia
Soares, Ana Caroline Silva [UNESP]
Buratini, Jose [UNESP]
Lodde, Valentina
Franciosi, Federica
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv University of Milan
Universidade Estadual Paulista (UNESP)
Reproductive Medicine Centre
dc.contributor.author.fl_str_mv Luciano, Alberto Maria
Barros, Rodrigo Garcia
Soares, Ana Caroline Silva [UNESP]
Buratini, Jose [UNESP]
Lodde, Valentina
Franciosi, Federica
dc.subject.por.fl_str_mv Chromatin
Cumulus cells
Gap junction
Germinal vesicle
In vitro oocyte growth
Intercellular communication
Meiotic arrest
Oocyte
Oogenesis
Prematuration
topic Chromatin
Cumulus cells
Gap junction
Germinal vesicle
In vitro oocyte growth
Intercellular communication
Meiotic arrest
Oocyte
Oogenesis
Prematuration
description The mammalian ovary is a large source of oocytes organized into follicles at various stages of folliculogenesis. However, only a limited number of them can be used for in vitro embryo production (IVEP), while most have yet to complete growth and development to attain full meiotic and embryonic developmental competence. While the in vitro growth of primordial follicles in the ovarian cortex has the potential to produce mature oocytes, it is still at an experimental stage. The population of early antral follicles (EAFs), instead, may represent a reserve of oocytes close to completing the growth phase, which might be more easily exploited in vitro and could increase the number of female gametes dedicated to IVEP. Here we present in vitro culture strategies that have been developed utilizing physiological parameters to support the specific needs of oocytes at distinct stages of differentiation, in order to expand the source of female gametes for IVEP by maximizing the attainment of fertilizable oocytes. Furthermore, these culture systems provide powerful tools to dissect the molecular processes that direct the final differentiation of the mammalian oocyte.
publishDate 2021
dc.date.none.fl_str_mv 2021-01-01
2022-05-01T03:42:47Z
2022-05-01T03:42:47Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/bookPart
format bookPart
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/978-1-0716-1246-0_1
Methods in Molecular Biology, v. 2273, p. 1-15.
1940-6029
1064-3745
http://hdl.handle.net/11449/233101
10.1007/978-1-0716-1246-0_1
2-s2.0-85101716304
url http://dx.doi.org/10.1007/978-1-0716-1246-0_1
http://hdl.handle.net/11449/233101
identifier_str_mv Methods in Molecular Biology, v. 2273, p. 1-15.
1940-6029
1064-3745
10.1007/978-1-0716-1246-0_1
2-s2.0-85101716304
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Methods in Molecular Biology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1-15
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129273645498368

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