How can micelle systems be rebuilt by a heating process?
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/226699 |
Resumo: | The aim of this work was to evaluate how an aqueous micellar system containing Amphotericin B (AmB) and sodium deoxycholate (DOC) can be rebuilt after heating treatment. Also, a review of the literature on the physicochemical and biological properties of this new system was conducted. Heated (AmB-DOC-H) and unheated (AmB-DOC) micelles were then diluted at four different concentrations (50 mg. L -1, 5 mg. L -1, 0.5 mg. L -1, and 0.05 mg. L -1) to perform physicochemical studies and a pharmacotoxicity assay, in which two cell models were used for the in vitro experiments: red blood cells (RBC) from human donors and Candida parapsilosis (Cp). While potassium (K +) and hemoglobin leakage from RBC were the parameters used to evaluate acute and chronic toxicity, respectively, the effcacy of AmB-DOC and AmB-DOC-H were assessed by K + leakage and cell survival rate from Cp. The spectral study revealed a slight change in the AmB-DOC aggregate peak from 327 nm to 323 nm, which is the peak for AmB-DOC-H. Although AmB-DOC and AmB-DOC-H exhibited different behavior for hemoglobin leakage, AmB-DOC produced higher leakage than AmB-DOC-H at high concentrations (from 5 mg. L -1). For K + leakage, both AmB-DOC and AmB-DOC-H showed a similar profle for both cell models, RBC and Cp (P < 0.05). AmB-DOC-H and AmB-DOC also revealed a similar profle of activity against Cp with an equivalent survival rate. In short, AmB-DOC-H showed much less toxicity than AmB-DOC, but remained as active as AmB-DOC against fungal cells. The results highlight the importance of this new procedure as a simple, inexpensive, and safe way to produce a new kind of micelle system for the treatment of systemic fungal infections. © 2012 Silva-Filho et al. |
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How can micelle systems be rebuilt by a heating process?MicellesNanotechnologyPre-heated amphotericin BSuper-aggregatesThe aim of this work was to evaluate how an aqueous micellar system containing Amphotericin B (AmB) and sodium deoxycholate (DOC) can be rebuilt after heating treatment. Also, a review of the literature on the physicochemical and biological properties of this new system was conducted. Heated (AmB-DOC-H) and unheated (AmB-DOC) micelles were then diluted at four different concentrations (50 mg. L -1, 5 mg. L -1, 0.5 mg. L -1, and 0.05 mg. L -1) to perform physicochemical studies and a pharmacotoxicity assay, in which two cell models were used for the in vitro experiments: red blood cells (RBC) from human donors and Candida parapsilosis (Cp). While potassium (K +) and hemoglobin leakage from RBC were the parameters used to evaluate acute and chronic toxicity, respectively, the effcacy of AmB-DOC and AmB-DOC-H were assessed by K + leakage and cell survival rate from Cp. The spectral study revealed a slight change in the AmB-DOC aggregate peak from 327 nm to 323 nm, which is the peak for AmB-DOC-H. Although AmB-DOC and AmB-DOC-H exhibited different behavior for hemoglobin leakage, AmB-DOC produced higher leakage than AmB-DOC-H at high concentrations (from 5 mg. L -1). For K + leakage, both AmB-DOC and AmB-DOC-H showed a similar profle for both cell models, RBC and Cp (P < 0.05). AmB-DOC-H and AmB-DOC also revealed a similar profle of activity against Cp with an equivalent survival rate. In short, AmB-DOC-H showed much less toxicity than AmB-DOC, but remained as active as AmB-DOC against fungal cells. The results highlight the importance of this new procedure as a simple, inexpensive, and safe way to produce a new kind of micelle system for the treatment of systemic fungal infections. © 2012 Silva-Filho et al.Dispersed Systems Laboratory Federal University of rio grande do Norte (UFrN), Rio grande do Norte, 8948 NatalDepartment of Pharmacy UFrN, Natal, Rio grande do NorteDepartment of experimental Surgery UFrN, Natal, rio grande do NorteDepartment of Drugs and Medicines College of Pharmaceutical Sciences (UNeSP), Araraquara, São PauloDepartment of Drugs and Medicines College of Pharmaceutical Sciences (UNeSP), Araraquara, São PauloFederal University of rio grande do Norte (UFrN)UFrNUniversidade Estadual Paulista (UNESP)da Silva-Filho, Miguel AdelinoSiqueira, Scheyla Daniela Vieira da SilvaFreire, Larissa Bandeirade Araújo, Ivonete Batistade Holandae e Silva, Káttya Gyselleda Medeiros, Aldo CunhaAraújo-Filho, Iramide Oliveira, Anselmo Gomes [UNESP]do Egito, Eryvaldo Sócrates Tabosa2022-04-29T02:43:14Z2022-04-29T02:43:14Z2012-02-10info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article141-150International Journal of Nanomedicine, v. 7, p. 141-150.1176-91141178-2013http://hdl.handle.net/11449/2266992-s2.0-84856639968Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Nanomedicineinfo:eu-repo/semantics/openAccess2024-06-24T13:46:11Zoai:repositorio.unesp.br:11449/226699Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:34:29.495158Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
How can micelle systems be rebuilt by a heating process? |
title |
How can micelle systems be rebuilt by a heating process? |
spellingShingle |
How can micelle systems be rebuilt by a heating process? da Silva-Filho, Miguel Adelino Micelles Nanotechnology Pre-heated amphotericin B Super-aggregates |
title_short |
How can micelle systems be rebuilt by a heating process? |
title_full |
How can micelle systems be rebuilt by a heating process? |
title_fullStr |
How can micelle systems be rebuilt by a heating process? |
title_full_unstemmed |
How can micelle systems be rebuilt by a heating process? |
title_sort |
How can micelle systems be rebuilt by a heating process? |
author |
da Silva-Filho, Miguel Adelino |
author_facet |
da Silva-Filho, Miguel Adelino Siqueira, Scheyla Daniela Vieira da Silva Freire, Larissa Bandeira de Araújo, Ivonete Batista de Holandae e Silva, Káttya Gyselle da Medeiros, Aldo Cunha Araújo-Filho, Irami de Oliveira, Anselmo Gomes [UNESP] do Egito, Eryvaldo Sócrates Tabosa |
author_role |
author |
author2 |
Siqueira, Scheyla Daniela Vieira da Silva Freire, Larissa Bandeira de Araújo, Ivonete Batista de Holandae e Silva, Káttya Gyselle da Medeiros, Aldo Cunha Araújo-Filho, Irami de Oliveira, Anselmo Gomes [UNESP] do Egito, Eryvaldo Sócrates Tabosa |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Federal University of rio grande do Norte (UFrN) UFrN Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
da Silva-Filho, Miguel Adelino Siqueira, Scheyla Daniela Vieira da Silva Freire, Larissa Bandeira de Araújo, Ivonete Batista de Holandae e Silva, Káttya Gyselle da Medeiros, Aldo Cunha Araújo-Filho, Irami de Oliveira, Anselmo Gomes [UNESP] do Egito, Eryvaldo Sócrates Tabosa |
dc.subject.por.fl_str_mv |
Micelles Nanotechnology Pre-heated amphotericin B Super-aggregates |
topic |
Micelles Nanotechnology Pre-heated amphotericin B Super-aggregates |
description |
The aim of this work was to evaluate how an aqueous micellar system containing Amphotericin B (AmB) and sodium deoxycholate (DOC) can be rebuilt after heating treatment. Also, a review of the literature on the physicochemical and biological properties of this new system was conducted. Heated (AmB-DOC-H) and unheated (AmB-DOC) micelles were then diluted at four different concentrations (50 mg. L -1, 5 mg. L -1, 0.5 mg. L -1, and 0.05 mg. L -1) to perform physicochemical studies and a pharmacotoxicity assay, in which two cell models were used for the in vitro experiments: red blood cells (RBC) from human donors and Candida parapsilosis (Cp). While potassium (K +) and hemoglobin leakage from RBC were the parameters used to evaluate acute and chronic toxicity, respectively, the effcacy of AmB-DOC and AmB-DOC-H were assessed by K + leakage and cell survival rate from Cp. The spectral study revealed a slight change in the AmB-DOC aggregate peak from 327 nm to 323 nm, which is the peak for AmB-DOC-H. Although AmB-DOC and AmB-DOC-H exhibited different behavior for hemoglobin leakage, AmB-DOC produced higher leakage than AmB-DOC-H at high concentrations (from 5 mg. L -1). For K + leakage, both AmB-DOC and AmB-DOC-H showed a similar profle for both cell models, RBC and Cp (P < 0.05). AmB-DOC-H and AmB-DOC also revealed a similar profle of activity against Cp with an equivalent survival rate. In short, AmB-DOC-H showed much less toxicity than AmB-DOC, but remained as active as AmB-DOC against fungal cells. The results highlight the importance of this new procedure as a simple, inexpensive, and safe way to produce a new kind of micelle system for the treatment of systemic fungal infections. © 2012 Silva-Filho et al. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-02-10 2022-04-29T02:43:14Z 2022-04-29T02:43:14Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
International Journal of Nanomedicine, v. 7, p. 141-150. 1176-9114 1178-2013 http://hdl.handle.net/11449/226699 2-s2.0-84856639968 |
identifier_str_mv |
International Journal of Nanomedicine, v. 7, p. 141-150. 1176-9114 1178-2013 2-s2.0-84856639968 |
url |
http://hdl.handle.net/11449/226699 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal of Nanomedicine |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
141-150 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129221274370048 |