Serological point-of-care and label-free capacitive diagnosis of dengue virus infection

Detalhes bibliográficos
Autor(a) principal: Cecchetto, Juliana [UNESP]
Data de Publicação: 2020
Outros Autores: Santos, Adriano [UNESP], Mondini, Adriano [UNESP], Cilli, Eduardo M. [UNESP], Bueno, Paulo R. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.bios.2019.111972
http://hdl.handle.net/11449/201405
Resumo: Dengue non-structural protein 1 (NS1 DENV) is considered a biomarker for dengue fever in an early stage. A sensitive and rapid assay for distinguishing positive from negative dengue infection samples is imperative for epidemic control and public health in tropical regions because it enables the development of instantaneous updatable databases and effective surveillance systems. Presently, we successfully report, for the first time, the use of the electrochemical capacitive method for the detection of NS1 DENV biomarker in human serum samples. By using a ferrocene-tagged peptide modified surface containing anti-NS1 as the receptor, it was possible to differentiate positive from negative samples with a p < 0.01 in a reagentless and label-free capacitive format. This capacitive assay had a cut-off of 1.36% (confidence interval of 99.99%); it therefore opens new avenues for developing miniature label-free electrochemical devices for infectious diseases.
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spelling Serological point-of-care and label-free capacitive diagnosis of dengue virus infectionCapacitive biosensorsElectrochemical capacitanceElectrochemical impedance spectroscopyLabel-free diagnosticsMolecular assayingPoint-of-care diagnosticsDengue non-structural protein 1 (NS1 DENV) is considered a biomarker for dengue fever in an early stage. A sensitive and rapid assay for distinguishing positive from negative dengue infection samples is imperative for epidemic control and public health in tropical regions because it enables the development of instantaneous updatable databases and effective surveillance systems. Presently, we successfully report, for the first time, the use of the electrochemical capacitive method for the detection of NS1 DENV biomarker in human serum samples. By using a ferrocene-tagged peptide modified surface containing anti-NS1 as the receptor, it was possible to differentiate positive from negative samples with a p < 0.01 in a reagentless and label-free capacitive format. This capacitive assay had a cut-off of 1.36% (confidence interval of 99.99%); it therefore opens new avenues for developing miniature label-free electrochemical devices for infectious diseases.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Institute of Chemistry São Paulo State University (UNESP), CP 355, São PauloPharmaceutical Faculty São Paulo State University (UNESP), CP 355, São PauloInstitute of Chemistry São Paulo State University (UNESP), CP 355, São PauloPharmaceutical Faculty São Paulo State University (UNESP), CP 355, São PauloFAPESP: 2016/17185-1Universidade Estadual Paulista (Unesp)Cecchetto, Juliana [UNESP]Santos, Adriano [UNESP]Mondini, Adriano [UNESP]Cilli, Eduardo M. [UNESP]Bueno, Paulo R. [UNESP]2020-12-12T02:31:41Z2020-12-12T02:31:41Z2020-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.bios.2019.111972Biosensors and Bioelectronics, v. 151.1873-42350956-5663http://hdl.handle.net/11449/20140510.1016/j.bios.2019.1119722-s2.0-85076707182Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiosensors and Bioelectronicsinfo:eu-repo/semantics/openAccess2021-10-22T18:21:04Zoai:repositorio.unesp.br:11449/201405Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-22T18:21:04Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Serological point-of-care and label-free capacitive diagnosis of dengue virus infection
title Serological point-of-care and label-free capacitive diagnosis of dengue virus infection
spellingShingle Serological point-of-care and label-free capacitive diagnosis of dengue virus infection
Cecchetto, Juliana [UNESP]
Capacitive biosensors
Electrochemical capacitance
Electrochemical impedance spectroscopy
Label-free diagnostics
Molecular assaying
Point-of-care diagnostics
title_short Serological point-of-care and label-free capacitive diagnosis of dengue virus infection
title_full Serological point-of-care and label-free capacitive diagnosis of dengue virus infection
title_fullStr Serological point-of-care and label-free capacitive diagnosis of dengue virus infection
title_full_unstemmed Serological point-of-care and label-free capacitive diagnosis of dengue virus infection
title_sort Serological point-of-care and label-free capacitive diagnosis of dengue virus infection
author Cecchetto, Juliana [UNESP]
author_facet Cecchetto, Juliana [UNESP]
Santos, Adriano [UNESP]
Mondini, Adriano [UNESP]
Cilli, Eduardo M. [UNESP]
Bueno, Paulo R. [UNESP]
author_role author
author2 Santos, Adriano [UNESP]
Mondini, Adriano [UNESP]
Cilli, Eduardo M. [UNESP]
Bueno, Paulo R. [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Cecchetto, Juliana [UNESP]
Santos, Adriano [UNESP]
Mondini, Adriano [UNESP]
Cilli, Eduardo M. [UNESP]
Bueno, Paulo R. [UNESP]
dc.subject.por.fl_str_mv Capacitive biosensors
Electrochemical capacitance
Electrochemical impedance spectroscopy
Label-free diagnostics
Molecular assaying
Point-of-care diagnostics
topic Capacitive biosensors
Electrochemical capacitance
Electrochemical impedance spectroscopy
Label-free diagnostics
Molecular assaying
Point-of-care diagnostics
description Dengue non-structural protein 1 (NS1 DENV) is considered a biomarker for dengue fever in an early stage. A sensitive and rapid assay for distinguishing positive from negative dengue infection samples is imperative for epidemic control and public health in tropical regions because it enables the development of instantaneous updatable databases and effective surveillance systems. Presently, we successfully report, for the first time, the use of the electrochemical capacitive method for the detection of NS1 DENV biomarker in human serum samples. By using a ferrocene-tagged peptide modified surface containing anti-NS1 as the receptor, it was possible to differentiate positive from negative samples with a p < 0.01 in a reagentless and label-free capacitive format. This capacitive assay had a cut-off of 1.36% (confidence interval of 99.99%); it therefore opens new avenues for developing miniature label-free electrochemical devices for infectious diseases.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T02:31:41Z
2020-12-12T02:31:41Z
2020-03-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.bios.2019.111972
Biosensors and Bioelectronics, v. 151.
1873-4235
0956-5663
http://hdl.handle.net/11449/201405
10.1016/j.bios.2019.111972
2-s2.0-85076707182
url http://dx.doi.org/10.1016/j.bios.2019.111972
http://hdl.handle.net/11449/201405
identifier_str_mv Biosensors and Bioelectronics, v. 151.
1873-4235
0956-5663
10.1016/j.bios.2019.111972
2-s2.0-85076707182
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Biosensors and Bioelectronics
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1797789352887058432

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