Detection of QTLs associated to DBH in a Eucalyptus grandis x Eucalyptus Globulus monoprogeny

Detalhes bibliográficos
Autor(a) principal: Torres-Dini, Diego Gabriel [UNESP]
Data de Publicação: 2017
Tipo de documento: Tese
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/148905
Resumo: In Uruguay, reforestation with Eucalyptus sp. is of fundamental importance to produce paper, pulp and wood. The productivity of these continually grows due to application of breeding techniques, such as hybridization. This study aimed to investigate genetic parameters, productivity, stability, adaptability and to identify SNP markers associated with the diameter breast height (DBH) for to select Eucalypts grandis x Eucalyptus globulus full-sibs hybrid clones. The study was conducted in a clonal test, repeated at two different soils, in the state of Rio Negro, Uruguay. The population was phenotypically characterized to the DBH at 48 months of age and cambium tissues of each individual were sampled for genotyping with EuCHIP60K chip. The mean growth in DBH was similar between both places. The genotype-environment interaction was the simple type, with high genotype correlation in clones’ performance between environments (0.708), indicating the possibility of the same clones being selected for both places. Mean heritability between clones (0.724), coefficient of individual genetic variation (10.9%) and relative variation (0.916), showed the possibility of obtaining gains by selecting clones with higher growth, which was estimated in 3.1% for both sites together. A total of 15,196 markers SNPs were used in the genomic selection for the DBH, but after cleaning of SNPs data, the number was reduced for 15,196 (23.5%). The predictive capacity was expected to be low or negative (-0.15) for this population given the population size (78 individuals). We used the model rrBLUP with a validation of Jackknife. The model do not showed precision to predict the DBH. These results were consistent with theoretical expectations, which indicate that it is necessary to have an improvement population of at least 1,000 phenotyped and genotyped individuals. The DBH is the most important trait in the breeding of the genus Eucalyptus. However its quantitative nature added to the time necessary for this phenotype to develop makes the early detection of this trait are difficult. The identification of molecular markers associated with quantitative phenotypes is a good choice for the identification of QTLs that will help the early detection of individuals with high DBH. Significant markers associated to DBH , were indentificated into the chromosome 6, suggesting the presence of a QTL in this chromosome. Since they are clones originated from vegetative propagation and a full-sibs single-progeny, they should preferably be used for reforestation based on their cloning, since mating between clones can generate endogamy by biparental inbreeding. The utilization of SNPs helped to confirm the degree of parentage between the clones as well as clonal identity control.
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spelling Detection of QTLs associated to DBH in a Eucalyptus grandis x Eucalyptus Globulus monoprogenyDetecção de QTL associado a DAP em Eucaliptus grandis x Eucaliptus Globulus monoprogênieEucalyptus breedingQuantitative geneticsGenomic Wide Prediction (GWP)Genomic Wide Association (GWAS)SNPs MarkersUruguayIn Uruguay, reforestation with Eucalyptus sp. is of fundamental importance to produce paper, pulp and wood. The productivity of these continually grows due to application of breeding techniques, such as hybridization. This study aimed to investigate genetic parameters, productivity, stability, adaptability and to identify SNP markers associated with the diameter breast height (DBH) for to select Eucalypts grandis x Eucalyptus globulus full-sibs hybrid clones. The study was conducted in a clonal test, repeated at two different soils, in the state of Rio Negro, Uruguay. The population was phenotypically characterized to the DBH at 48 months of age and cambium tissues of each individual were sampled for genotyping with EuCHIP60K chip. The mean growth in DBH was similar between both places. The genotype-environment interaction was the simple type, with high genotype correlation in clones’ performance between environments (0.708), indicating the possibility of the same clones being selected for both places. Mean heritability between clones (0.724), coefficient of individual genetic variation (10.9%) and relative variation (0.916), showed the possibility of obtaining gains by selecting clones with higher growth, which was estimated in 3.1% for both sites together. A total of 15,196 markers SNPs were used in the genomic selection for the DBH, but after cleaning of SNPs data, the number was reduced for 15,196 (23.5%). The predictive capacity was expected to be low or negative (-0.15) for this population given the population size (78 individuals). We used the model rrBLUP with a validation of Jackknife. The model do not showed precision to predict the DBH. These results were consistent with theoretical expectations, which indicate that it is necessary to have an improvement population of at least 1,000 phenotyped and genotyped individuals. The DBH is the most important trait in the breeding of the genus Eucalyptus. However its quantitative nature added to the time necessary for this phenotype to develop makes the early detection of this trait are difficult. The identification of molecular markers associated with quantitative phenotypes is a good choice for the identification of QTLs that will help the early detection of individuals with high DBH. Significant markers associated to DBH , were indentificated into the chromosome 6, suggesting the presence of a QTL in this chromosome. Since they are clones originated from vegetative propagation and a full-sibs single-progeny, they should preferably be used for reforestation based on their cloning, since mating between clones can generate endogamy by biparental inbreeding. The utilization of SNPs helped to confirm the degree of parentage between the clones as well as clonal identity control.OutraUniversidade Estadual Paulista (Unesp)Sebbenn, Alexandre Magno [UNESP]Universidade Estadual Paulista (Unesp)Torres-Dini, Diego Gabriel [UNESP]2017-03-07T13:42:52Z2017-03-07T13:42:52Z2017-02-03info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfhttp://hdl.handle.net/11449/14890500088138033004099079P1enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESP2024-07-10T19:59:01Zoai:repositorio.unesp.br:11449/148905Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:27:40.773325Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Detection of QTLs associated to DBH in a Eucalyptus grandis x Eucalyptus Globulus monoprogeny
Detecção de QTL associado a DAP em Eucaliptus grandis x Eucaliptus Globulus monoprogênie
title Detection of QTLs associated to DBH in a Eucalyptus grandis x Eucalyptus Globulus monoprogeny
spellingShingle Detection of QTLs associated to DBH in a Eucalyptus grandis x Eucalyptus Globulus monoprogeny
Torres-Dini, Diego Gabriel [UNESP]
Eucalyptus breeding
Quantitative genetics
Genomic Wide Prediction (GWP)
Genomic Wide Association (GWAS)
SNPs Markers
Uruguay
title_short Detection of QTLs associated to DBH in a Eucalyptus grandis x Eucalyptus Globulus monoprogeny
title_full Detection of QTLs associated to DBH in a Eucalyptus grandis x Eucalyptus Globulus monoprogeny
title_fullStr Detection of QTLs associated to DBH in a Eucalyptus grandis x Eucalyptus Globulus monoprogeny
title_full_unstemmed Detection of QTLs associated to DBH in a Eucalyptus grandis x Eucalyptus Globulus monoprogeny
title_sort Detection of QTLs associated to DBH in a Eucalyptus grandis x Eucalyptus Globulus monoprogeny
author Torres-Dini, Diego Gabriel [UNESP]
author_facet Torres-Dini, Diego Gabriel [UNESP]
author_role author
dc.contributor.none.fl_str_mv Sebbenn, Alexandre Magno [UNESP]
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Torres-Dini, Diego Gabriel [UNESP]
dc.subject.por.fl_str_mv Eucalyptus breeding
Quantitative genetics
Genomic Wide Prediction (GWP)
Genomic Wide Association (GWAS)
SNPs Markers
Uruguay
topic Eucalyptus breeding
Quantitative genetics
Genomic Wide Prediction (GWP)
Genomic Wide Association (GWAS)
SNPs Markers
Uruguay
description In Uruguay, reforestation with Eucalyptus sp. is of fundamental importance to produce paper, pulp and wood. The productivity of these continually grows due to application of breeding techniques, such as hybridization. This study aimed to investigate genetic parameters, productivity, stability, adaptability and to identify SNP markers associated with the diameter breast height (DBH) for to select Eucalypts grandis x Eucalyptus globulus full-sibs hybrid clones. The study was conducted in a clonal test, repeated at two different soils, in the state of Rio Negro, Uruguay. The population was phenotypically characterized to the DBH at 48 months of age and cambium tissues of each individual were sampled for genotyping with EuCHIP60K chip. The mean growth in DBH was similar between both places. The genotype-environment interaction was the simple type, with high genotype correlation in clones’ performance between environments (0.708), indicating the possibility of the same clones being selected for both places. Mean heritability between clones (0.724), coefficient of individual genetic variation (10.9%) and relative variation (0.916), showed the possibility of obtaining gains by selecting clones with higher growth, which was estimated in 3.1% for both sites together. A total of 15,196 markers SNPs were used in the genomic selection for the DBH, but after cleaning of SNPs data, the number was reduced for 15,196 (23.5%). The predictive capacity was expected to be low or negative (-0.15) for this population given the population size (78 individuals). We used the model rrBLUP with a validation of Jackknife. The model do not showed precision to predict the DBH. These results were consistent with theoretical expectations, which indicate that it is necessary to have an improvement population of at least 1,000 phenotyped and genotyped individuals. The DBH is the most important trait in the breeding of the genus Eucalyptus. However its quantitative nature added to the time necessary for this phenotype to develop makes the early detection of this trait are difficult. The identification of molecular markers associated with quantitative phenotypes is a good choice for the identification of QTLs that will help the early detection of individuals with high DBH. Significant markers associated to DBH , were indentificated into the chromosome 6, suggesting the presence of a QTL in this chromosome. Since they are clones originated from vegetative propagation and a full-sibs single-progeny, they should preferably be used for reforestation based on their cloning, since mating between clones can generate endogamy by biparental inbreeding. The utilization of SNPs helped to confirm the degree of parentage between the clones as well as clonal identity control.
publishDate 2017
dc.date.none.fl_str_mv 2017-03-07T13:42:52Z
2017-03-07T13:42:52Z
2017-02-03
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/11449/148905
000881380
33004099079P1
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dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual Paulista (Unesp)
publisher.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
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instname_str Universidade Estadual Paulista (UNESP)
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reponame_str Repositório Institucional da UNESP
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repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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