Fungal Screening for Potential PET Depolymerization
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/polym15061581 http://hdl.handle.net/11449/248696 |
Resumo: | Approximately 400 billion PET bottles are produced annually in the world, of which from 8 to 9 million tons are discarded in oceans. This requires developing strategies to urgently recycle them. PET recycling can be carried out using the microbial hydrolysis of polymers when monomers and oligomers are released. Exploring the metabolic activity of fungi is an environmentally friendly way to treat harmful polymeric waste and obtain the production of monomers. The present study addressed: (i) the investigation of potential of strains with the potential for the depolymerization of PET bottles from different manufacturers (crystallinity of 35.5 and 10.4%); (ii) the search for a culture medium that favors the depolymerization process; and (iii) gaining more knowledge on fungal enzymes that can be applied to PET recycling. Four strains (from 100 fungal strains) were found as promising for conversion into terephthalic acid from PET nanoparticles (npPET): Curvularia trifolii CBMAI 2111, Trichoderma sp. CBMAI 2071, Trichoderma atroviride CBMAI 2073, and Cladosporium cladosporioides CBMAI 2075. The fermentation assays in the presence of PET led to the release of terephthalic acid in concentrations above 12 ppm. Biodegradation was also confirmed using mass variation analyses (reducing mass), scanning electron microscopy (SEM) that showed evidence of material roughness, FTIR analysis that showed band modification, enzymatic activities detected for lipase, and esterase and cutinase, confirmed by monomers/oligomers quantification using high performance liquid chromatography (HPLC-UV). Based on the microbial strains PET depolymerization, the results are promising for the exploration of the selected microbial strain. |
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Fungal Screening for Potential PET Depolymerizationbiodegradationenzymatic catalysispolymersterephthalic acidApproximately 400 billion PET bottles are produced annually in the world, of which from 8 to 9 million tons are discarded in oceans. This requires developing strategies to urgently recycle them. PET recycling can be carried out using the microbial hydrolysis of polymers when monomers and oligomers are released. Exploring the metabolic activity of fungi is an environmentally friendly way to treat harmful polymeric waste and obtain the production of monomers. The present study addressed: (i) the investigation of potential of strains with the potential for the depolymerization of PET bottles from different manufacturers (crystallinity of 35.5 and 10.4%); (ii) the search for a culture medium that favors the depolymerization process; and (iii) gaining more knowledge on fungal enzymes that can be applied to PET recycling. Four strains (from 100 fungal strains) were found as promising for conversion into terephthalic acid from PET nanoparticles (npPET): Curvularia trifolii CBMAI 2111, Trichoderma sp. CBMAI 2071, Trichoderma atroviride CBMAI 2073, and Cladosporium cladosporioides CBMAI 2075. The fermentation assays in the presence of PET led to the release of terephthalic acid in concentrations above 12 ppm. Biodegradation was also confirmed using mass variation analyses (reducing mass), scanning electron microscopy (SEM) that showed evidence of material roughness, FTIR analysis that showed band modification, enzymatic activities detected for lipase, and esterase and cutinase, confirmed by monomers/oligomers quantification using high performance liquid chromatography (HPLC-UV). Based on the microbial strains PET depolymerization, the results are promising for the exploration of the selected microbial strain.PetrobrasEnvironmental Studies Center (CEA) São Paulo State University (UNESP), Av. 24-A, 1515, Bela Vista, SPCoordination of Natural Sciences Federal University of Maranhão (UFMA), Av. João Alberto, 700, MADepartment of Biotechnology RD Center PETROBRAS, Av. Horácio Macedo, 950, Ilha do Fundão, RJDivision of Microbial Resources CPQBA State University of Campinas (Unicamp), Rua Alexandre Cazellato, 999, SPInstitute of Chemistry State University of Campinas (Unicamp), P.O. Box 6154, SPDepartment of Biochemistry and Microbiology São Paulo State University (UNESP), Av. 24-A, 1515, Bela Vista, SPInstitute For Research in Bioenergy (IPBEN) São Paulo State University (UNESP), R. 10, 2527, Santana, SPEnvironmental Studies Center (CEA) São Paulo State University (UNESP), Av. 24-A, 1515, Bela Vista, SPDepartment of Biochemistry and Microbiology São Paulo State University (UNESP), Av. 24-A, 1515, Bela Vista, SPInstitute For Research in Bioenergy (IPBEN) São Paulo State University (UNESP), R. 10, 2527, Santana, SPPetrobras: 2012/00327-7Universidade Estadual Paulista (UNESP)Federal University of Maranhão (UFMA)PETROBRASUniversidade Estadual de Campinas (UNICAMP)Malafatti-Picca, Lusiane [UNESP]Bucioli, Elaine Cristina [UNESP]de Barros Chaves, Michel Ricardode Castro, Aline MachadoValoni, Érikade Oliveira, Valéria MaiaMarsaioli, Anita JocelyneGovone, José Silvio [UNESP]de Franceschi de Angelis, Dejanira [UNESP]Brienzo, Michel [UNESP]Attili-Angelis, Derlene [UNESP]2023-07-29T13:51:05Z2023-07-29T13:51:05Z2023-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/polym15061581Polymers, v. 15, n. 6, 2023.2073-4360http://hdl.handle.net/11449/24869610.3390/polym150615812-s2.0-85152629953Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPolymersinfo:eu-repo/semantics/openAccess2023-07-29T13:51:05Zoai:repositorio.unesp.br:11449/248696Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:44:18.783081Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Fungal Screening for Potential PET Depolymerization |
title |
Fungal Screening for Potential PET Depolymerization |
spellingShingle |
Fungal Screening for Potential PET Depolymerization Malafatti-Picca, Lusiane [UNESP] biodegradation enzymatic catalysis polymers terephthalic acid |
title_short |
Fungal Screening for Potential PET Depolymerization |
title_full |
Fungal Screening for Potential PET Depolymerization |
title_fullStr |
Fungal Screening for Potential PET Depolymerization |
title_full_unstemmed |
Fungal Screening for Potential PET Depolymerization |
title_sort |
Fungal Screening for Potential PET Depolymerization |
author |
Malafatti-Picca, Lusiane [UNESP] |
author_facet |
Malafatti-Picca, Lusiane [UNESP] Bucioli, Elaine Cristina [UNESP] de Barros Chaves, Michel Ricardo de Castro, Aline Machado Valoni, Érika de Oliveira, Valéria Maia Marsaioli, Anita Jocelyne Govone, José Silvio [UNESP] de Franceschi de Angelis, Dejanira [UNESP] Brienzo, Michel [UNESP] Attili-Angelis, Derlene [UNESP] |
author_role |
author |
author2 |
Bucioli, Elaine Cristina [UNESP] de Barros Chaves, Michel Ricardo de Castro, Aline Machado Valoni, Érika de Oliveira, Valéria Maia Marsaioli, Anita Jocelyne Govone, José Silvio [UNESP] de Franceschi de Angelis, Dejanira [UNESP] Brienzo, Michel [UNESP] Attili-Angelis, Derlene [UNESP] |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Federal University of Maranhão (UFMA) PETROBRAS Universidade Estadual de Campinas (UNICAMP) |
dc.contributor.author.fl_str_mv |
Malafatti-Picca, Lusiane [UNESP] Bucioli, Elaine Cristina [UNESP] de Barros Chaves, Michel Ricardo de Castro, Aline Machado Valoni, Érika de Oliveira, Valéria Maia Marsaioli, Anita Jocelyne Govone, José Silvio [UNESP] de Franceschi de Angelis, Dejanira [UNESP] Brienzo, Michel [UNESP] Attili-Angelis, Derlene [UNESP] |
dc.subject.por.fl_str_mv |
biodegradation enzymatic catalysis polymers terephthalic acid |
topic |
biodegradation enzymatic catalysis polymers terephthalic acid |
description |
Approximately 400 billion PET bottles are produced annually in the world, of which from 8 to 9 million tons are discarded in oceans. This requires developing strategies to urgently recycle them. PET recycling can be carried out using the microbial hydrolysis of polymers when monomers and oligomers are released. Exploring the metabolic activity of fungi is an environmentally friendly way to treat harmful polymeric waste and obtain the production of monomers. The present study addressed: (i) the investigation of potential of strains with the potential for the depolymerization of PET bottles from different manufacturers (crystallinity of 35.5 and 10.4%); (ii) the search for a culture medium that favors the depolymerization process; and (iii) gaining more knowledge on fungal enzymes that can be applied to PET recycling. Four strains (from 100 fungal strains) were found as promising for conversion into terephthalic acid from PET nanoparticles (npPET): Curvularia trifolii CBMAI 2111, Trichoderma sp. CBMAI 2071, Trichoderma atroviride CBMAI 2073, and Cladosporium cladosporioides CBMAI 2075. The fermentation assays in the presence of PET led to the release of terephthalic acid in concentrations above 12 ppm. Biodegradation was also confirmed using mass variation analyses (reducing mass), scanning electron microscopy (SEM) that showed evidence of material roughness, FTIR analysis that showed band modification, enzymatic activities detected for lipase, and esterase and cutinase, confirmed by monomers/oligomers quantification using high performance liquid chromatography (HPLC-UV). Based on the microbial strains PET depolymerization, the results are promising for the exploration of the selected microbial strain. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T13:51:05Z 2023-07-29T13:51:05Z 2023-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/polym15061581 Polymers, v. 15, n. 6, 2023. 2073-4360 http://hdl.handle.net/11449/248696 10.3390/polym15061581 2-s2.0-85152629953 |
url |
http://dx.doi.org/10.3390/polym15061581 http://hdl.handle.net/11449/248696 |
identifier_str_mv |
Polymers, v. 15, n. 6, 2023. 2073-4360 10.3390/polym15061581 2-s2.0-85152629953 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Polymers |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129240462262272 |