Karyotype diversity and patterns of chromosomal evolution in Eigenmannia (Teleostei, Gymnotiformes, Sternopygidae)
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3897/CompCytogen.v8i4.8396 http://hdl.handle.net/11449/117534 |
Resumo: | Conventional (Giemsa, C-banding, Ag - NORs) and molecular [5S rDNA, 18S rDNA, (TTAGGG)(n)] cytogenetic techniques were employed to study six species of the genus Eigenmannia Jordan & Evermann, 1896. They exhibited diploid chromosome numbers ranging from 2n=28 (Eigenmannia sp. 1) to 2n=38 (E. virescens (Valenciennes, 1836)). The C-banding results revealed that species with the lowest 2n have less heterochromatin content and that morphologically differentiated sex chromosomes observed in two species showed distinct patterns of heterochromatin. While the X-1, X-2 and Y-chromosomes of Eigenmannia sp. 2 showed only centromeric heterochromatin, the XY sex chromosomes of E. virescens possessed large heterochromatic blocks in the terminal position, particularly on the X chromosome. The nucleolus organizer regions (NORs) were located in different positions when compared to the 5S rDNA sites. Additionally, the presence of minor ribosomal gene sites on the sex chromosome pair of E. virescens represented a new type of the sex chromosomes in this group. The telomeric probe (TTAGGG)(n) hybridized to the terminal portion of all chromosomes in all species examined however, interstitial telomeric sites were found in the metacentric pair No. 2 in Eigenmannia sp. 1. The analyzes confirmed some hypotheses about karyotype evolution in the genus Eigenmannia, and brought new information about the distribution of the genetic material in the chromosomes of the samples analyzed providing new insights for understanding the process differentiation in the genomes of species under study. |
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Karyotype diversity and patterns of chromosomal evolution in Eigenmannia (Teleostei, Gymnotiformes, Sternopygidae)Fish comparative cytogeneticselectric fisheschromosome bandingrDNA variabilitysex chromosomesConventional (Giemsa, C-banding, Ag - NORs) and molecular [5S rDNA, 18S rDNA, (TTAGGG)(n)] cytogenetic techniques were employed to study six species of the genus Eigenmannia Jordan & Evermann, 1896. They exhibited diploid chromosome numbers ranging from 2n=28 (Eigenmannia sp. 1) to 2n=38 (E. virescens (Valenciennes, 1836)). The C-banding results revealed that species with the lowest 2n have less heterochromatin content and that morphologically differentiated sex chromosomes observed in two species showed distinct patterns of heterochromatin. While the X-1, X-2 and Y-chromosomes of Eigenmannia sp. 2 showed only centromeric heterochromatin, the XY sex chromosomes of E. virescens possessed large heterochromatic blocks in the terminal position, particularly on the X chromosome. The nucleolus organizer regions (NORs) were located in different positions when compared to the 5S rDNA sites. Additionally, the presence of minor ribosomal gene sites on the sex chromosome pair of E. virescens represented a new type of the sex chromosomes in this group. The telomeric probe (TTAGGG)(n) hybridized to the terminal portion of all chromosomes in all species examined however, interstitial telomeric sites were found in the metacentric pair No. 2 in Eigenmannia sp. 1. The analyzes confirmed some hypotheses about karyotype evolution in the genus Eigenmannia, and brought new information about the distribution of the genetic material in the chromosomes of the samples analyzed providing new insights for understanding the process differentiation in the genomes of species under study.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Estadual Paulista UNESP, Lab Biol Genet Peixes, Inst Biociencias Botucatu, Dept Morfol, Botucatu, SP, BrazilUniv Estadual Paulista UNESP, Lab Biol Genet Peixes, Inst Biociencias Botucatu, Dept Morfol, Botucatu, SP, BrazilPensoft PublUniversidade Estadual Paulista (Unesp)Sene, Viviani Franca de [UNESP]Pansonato-Alves, Jose Carlos [UNESP]Utsunomia, Ricardo [UNESP]Oliveira, Claudio [UNESP]Foresti, Fausto [UNESP]2015-03-18T15:56:22Z2015-03-18T15:56:22Z2014-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article301-311application/pdfhttp://dx.doi.org/10.3897/CompCytogen.v8i4.8396Comparative Cytogenetics. Sofia: Pensoft Publ, v. 8, n. 4, p. 301-311, 2014.1993-0771http://hdl.handle.net/11449/11753410.3897/CompCytogen.v8i4.8396WOS:000345253300005WOS000345253300005.pdf0804793944846367Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengComparative Cytogenetics1.3190,431info:eu-repo/semantics/openAccess2023-10-17T06:04:52Zoai:repositorio.unesp.br:11449/117534Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:09:50.620997Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Karyotype diversity and patterns of chromosomal evolution in Eigenmannia (Teleostei, Gymnotiformes, Sternopygidae) |
title |
Karyotype diversity and patterns of chromosomal evolution in Eigenmannia (Teleostei, Gymnotiformes, Sternopygidae) |
spellingShingle |
Karyotype diversity and patterns of chromosomal evolution in Eigenmannia (Teleostei, Gymnotiformes, Sternopygidae) Sene, Viviani Franca de [UNESP] Fish comparative cytogenetics electric fishes chromosome banding rDNA variability sex chromosomes |
title_short |
Karyotype diversity and patterns of chromosomal evolution in Eigenmannia (Teleostei, Gymnotiformes, Sternopygidae) |
title_full |
Karyotype diversity and patterns of chromosomal evolution in Eigenmannia (Teleostei, Gymnotiformes, Sternopygidae) |
title_fullStr |
Karyotype diversity and patterns of chromosomal evolution in Eigenmannia (Teleostei, Gymnotiformes, Sternopygidae) |
title_full_unstemmed |
Karyotype diversity and patterns of chromosomal evolution in Eigenmannia (Teleostei, Gymnotiformes, Sternopygidae) |
title_sort |
Karyotype diversity and patterns of chromosomal evolution in Eigenmannia (Teleostei, Gymnotiformes, Sternopygidae) |
author |
Sene, Viviani Franca de [UNESP] |
author_facet |
Sene, Viviani Franca de [UNESP] Pansonato-Alves, Jose Carlos [UNESP] Utsunomia, Ricardo [UNESP] Oliveira, Claudio [UNESP] Foresti, Fausto [UNESP] |
author_role |
author |
author2 |
Pansonato-Alves, Jose Carlos [UNESP] Utsunomia, Ricardo [UNESP] Oliveira, Claudio [UNESP] Foresti, Fausto [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Sene, Viviani Franca de [UNESP] Pansonato-Alves, Jose Carlos [UNESP] Utsunomia, Ricardo [UNESP] Oliveira, Claudio [UNESP] Foresti, Fausto [UNESP] |
dc.subject.por.fl_str_mv |
Fish comparative cytogenetics electric fishes chromosome banding rDNA variability sex chromosomes |
topic |
Fish comparative cytogenetics electric fishes chromosome banding rDNA variability sex chromosomes |
description |
Conventional (Giemsa, C-banding, Ag - NORs) and molecular [5S rDNA, 18S rDNA, (TTAGGG)(n)] cytogenetic techniques were employed to study six species of the genus Eigenmannia Jordan & Evermann, 1896. They exhibited diploid chromosome numbers ranging from 2n=28 (Eigenmannia sp. 1) to 2n=38 (E. virescens (Valenciennes, 1836)). The C-banding results revealed that species with the lowest 2n have less heterochromatin content and that morphologically differentiated sex chromosomes observed in two species showed distinct patterns of heterochromatin. While the X-1, X-2 and Y-chromosomes of Eigenmannia sp. 2 showed only centromeric heterochromatin, the XY sex chromosomes of E. virescens possessed large heterochromatic blocks in the terminal position, particularly on the X chromosome. The nucleolus organizer regions (NORs) were located in different positions when compared to the 5S rDNA sites. Additionally, the presence of minor ribosomal gene sites on the sex chromosome pair of E. virescens represented a new type of the sex chromosomes in this group. The telomeric probe (TTAGGG)(n) hybridized to the terminal portion of all chromosomes in all species examined however, interstitial telomeric sites were found in the metacentric pair No. 2 in Eigenmannia sp. 1. The analyzes confirmed some hypotheses about karyotype evolution in the genus Eigenmannia, and brought new information about the distribution of the genetic material in the chromosomes of the samples analyzed providing new insights for understanding the process differentiation in the genomes of species under study. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-01-01 2015-03-18T15:56:22Z 2015-03-18T15:56:22Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3897/CompCytogen.v8i4.8396 Comparative Cytogenetics. Sofia: Pensoft Publ, v. 8, n. 4, p. 301-311, 2014. 1993-0771 http://hdl.handle.net/11449/117534 10.3897/CompCytogen.v8i4.8396 WOS:000345253300005 WOS000345253300005.pdf 0804793944846367 |
url |
http://dx.doi.org/10.3897/CompCytogen.v8i4.8396 http://hdl.handle.net/11449/117534 |
identifier_str_mv |
Comparative Cytogenetics. Sofia: Pensoft Publ, v. 8, n. 4, p. 301-311, 2014. 1993-0771 10.3897/CompCytogen.v8i4.8396 WOS:000345253300005 WOS000345253300005.pdf 0804793944846367 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Comparative Cytogenetics 1.319 0,431 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
301-311 application/pdf |
dc.publisher.none.fl_str_mv |
Pensoft Publ |
publisher.none.fl_str_mv |
Pensoft Publ |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128470523314176 |