Efficacy evaluation of a novel oral silica-based vaccine in inducing mucosal immunity against Mycoplasma hyopneumoniae
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.rvsc.2023.03.018 http://hdl.handle.net/11449/247078 |
Resumo: | Mycoplasma hyopneumoniae, the main etiological agent of Porcine Enzootic Pneumonia, is widely spread in swine production worldwide. Its prevention is of great interest for the productive system, since its colonization in the lung tissue leads to intense production losses. This study aimed to compare the M. hyopneumoniae shedding and acute-phase response in 30 pigs submitted to different vaccination protocols: an experimental oral vaccine using a nanostructured mesoporous silica (SBA-15) as adjuvant (n = 10); an intramuscular commercially available vaccine at 24 days of age (n = 10); and a control group (n = 10) following experimental challenge with M. hyopneumoniae. Laryngeal and nasal swabs were collected weekly and oral fluids were collected at 7, 10, 14, 17, 23, 28, 35, 42, and 49 days post-infection to monitor pathogen excretion by qPCR. Nasal swabs were also used to detect anti-M. hyopneumoniae IgA by ELISA. Blood samples were collected for monitoring acute phase proteins. The antibody response was observed in both immunized groups seven days after vaccination, while the control group became positive for this immunoglobulin at 4 weeks after challenge. Lung lesion score was similar in the immunized groups, and lower than that observed in the control. SBA-15-adjuvanted oral vaccine provided immunological response, decreased shedding of M. hyopneumoniae and led to mucosal protection confirmed by the reduced pulmonary lesions. This study provides useful data for future development of vaccines against M. hyopneumoniae. |
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Efficacy evaluation of a novel oral silica-based vaccine in inducing mucosal immunity against Mycoplasma hyopneumoniaeEnzootic pneumoniaNanoparticlesOral vaccineMycoplasma hyopneumoniae, the main etiological agent of Porcine Enzootic Pneumonia, is widely spread in swine production worldwide. Its prevention is of great interest for the productive system, since its colonization in the lung tissue leads to intense production losses. This study aimed to compare the M. hyopneumoniae shedding and acute-phase response in 30 pigs submitted to different vaccination protocols: an experimental oral vaccine using a nanostructured mesoporous silica (SBA-15) as adjuvant (n = 10); an intramuscular commercially available vaccine at 24 days of age (n = 10); and a control group (n = 10) following experimental challenge with M. hyopneumoniae. Laryngeal and nasal swabs were collected weekly and oral fluids were collected at 7, 10, 14, 17, 23, 28, 35, 42, and 49 days post-infection to monitor pathogen excretion by qPCR. Nasal swabs were also used to detect anti-M. hyopneumoniae IgA by ELISA. Blood samples were collected for monitoring acute phase proteins. The antibody response was observed in both immunized groups seven days after vaccination, while the control group became positive for this immunoglobulin at 4 weeks after challenge. Lung lesion score was similar in the immunized groups, and lower than that observed in the control. SBA-15-adjuvanted oral vaccine provided immunological response, decreased shedding of M. hyopneumoniae and led to mucosal protection confirmed by the reduced pulmonary lesions. This study provides useful data for future development of vaccines against M. hyopneumoniae.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)São Paulo State University (Unesp) School of Agricultural and Veterinarian SciencesDepartment of Chemistry Federal University of São Paulo (UNIFESP), SPButantan InstituteUniversity of São Paulo (USP) Physics InstituteSão Paulo State University (Unesp) School of Agricultural and Veterinarian SciencesFAPESP: #2021/11914-0CNPq: #316447/2021-8FAPESP: 2017/17844-8FAPESP: 2018/12742-5FAPESP: 2019/19710-4FAPESP: 2020/04707-5FAPESP: 2020/08028-5Universidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Butantan InstituteFerreira, Geovana C. [UNESP]Sanches, Thainara V.C. [UNESP]Mechler-Dreibi, Marina L. [UNESP]Almeida, Henrique M.S. [UNESP]Storino, Gabriel Y. [UNESP]Sonalio, Karina [UNESP]Petri, Fernando A.M. [UNESP]Martins, Tereza S.Cides da Silva, Luís CarlosMontassier, Hélio J. [UNESP]Sant'Anna, Osvaldo A.Fantini, Márcia C.A.de Oliveira, Luís Guilherme [UNESP]2023-07-29T13:05:38Z2023-07-29T13:05:38Z2023-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article141-150http://dx.doi.org/10.1016/j.rvsc.2023.03.018Research in Veterinary Science, v. 158, p. 141-150.1532-26610034-5288http://hdl.handle.net/11449/24707810.1016/j.rvsc.2023.03.0182-s2.0-85151252565Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengResearch in Veterinary Scienceinfo:eu-repo/semantics/openAccess2023-07-29T13:05:38Zoai:repositorio.unesp.br:11449/247078Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:27:06.289748Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Efficacy evaluation of a novel oral silica-based vaccine in inducing mucosal immunity against Mycoplasma hyopneumoniae |
title |
Efficacy evaluation of a novel oral silica-based vaccine in inducing mucosal immunity against Mycoplasma hyopneumoniae |
spellingShingle |
Efficacy evaluation of a novel oral silica-based vaccine in inducing mucosal immunity against Mycoplasma hyopneumoniae Ferreira, Geovana C. [UNESP] Enzootic pneumonia Nanoparticles Oral vaccine |
title_short |
Efficacy evaluation of a novel oral silica-based vaccine in inducing mucosal immunity against Mycoplasma hyopneumoniae |
title_full |
Efficacy evaluation of a novel oral silica-based vaccine in inducing mucosal immunity against Mycoplasma hyopneumoniae |
title_fullStr |
Efficacy evaluation of a novel oral silica-based vaccine in inducing mucosal immunity against Mycoplasma hyopneumoniae |
title_full_unstemmed |
Efficacy evaluation of a novel oral silica-based vaccine in inducing mucosal immunity against Mycoplasma hyopneumoniae |
title_sort |
Efficacy evaluation of a novel oral silica-based vaccine in inducing mucosal immunity against Mycoplasma hyopneumoniae |
author |
Ferreira, Geovana C. [UNESP] |
author_facet |
Ferreira, Geovana C. [UNESP] Sanches, Thainara V.C. [UNESP] Mechler-Dreibi, Marina L. [UNESP] Almeida, Henrique M.S. [UNESP] Storino, Gabriel Y. [UNESP] Sonalio, Karina [UNESP] Petri, Fernando A.M. [UNESP] Martins, Tereza S. Cides da Silva, Luís Carlos Montassier, Hélio J. [UNESP] Sant'Anna, Osvaldo A. Fantini, Márcia C.A. de Oliveira, Luís Guilherme [UNESP] |
author_role |
author |
author2 |
Sanches, Thainara V.C. [UNESP] Mechler-Dreibi, Marina L. [UNESP] Almeida, Henrique M.S. [UNESP] Storino, Gabriel Y. [UNESP] Sonalio, Karina [UNESP] Petri, Fernando A.M. [UNESP] Martins, Tereza S. Cides da Silva, Luís Carlos Montassier, Hélio J. [UNESP] Sant'Anna, Osvaldo A. Fantini, Márcia C.A. de Oliveira, Luís Guilherme [UNESP] |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade de São Paulo (USP) Butantan Institute |
dc.contributor.author.fl_str_mv |
Ferreira, Geovana C. [UNESP] Sanches, Thainara V.C. [UNESP] Mechler-Dreibi, Marina L. [UNESP] Almeida, Henrique M.S. [UNESP] Storino, Gabriel Y. [UNESP] Sonalio, Karina [UNESP] Petri, Fernando A.M. [UNESP] Martins, Tereza S. Cides da Silva, Luís Carlos Montassier, Hélio J. [UNESP] Sant'Anna, Osvaldo A. Fantini, Márcia C.A. de Oliveira, Luís Guilherme [UNESP] |
dc.subject.por.fl_str_mv |
Enzootic pneumonia Nanoparticles Oral vaccine |
topic |
Enzootic pneumonia Nanoparticles Oral vaccine |
description |
Mycoplasma hyopneumoniae, the main etiological agent of Porcine Enzootic Pneumonia, is widely spread in swine production worldwide. Its prevention is of great interest for the productive system, since its colonization in the lung tissue leads to intense production losses. This study aimed to compare the M. hyopneumoniae shedding and acute-phase response in 30 pigs submitted to different vaccination protocols: an experimental oral vaccine using a nanostructured mesoporous silica (SBA-15) as adjuvant (n = 10); an intramuscular commercially available vaccine at 24 days of age (n = 10); and a control group (n = 10) following experimental challenge with M. hyopneumoniae. Laryngeal and nasal swabs were collected weekly and oral fluids were collected at 7, 10, 14, 17, 23, 28, 35, 42, and 49 days post-infection to monitor pathogen excretion by qPCR. Nasal swabs were also used to detect anti-M. hyopneumoniae IgA by ELISA. Blood samples were collected for monitoring acute phase proteins. The antibody response was observed in both immunized groups seven days after vaccination, while the control group became positive for this immunoglobulin at 4 weeks after challenge. Lung lesion score was similar in the immunized groups, and lower than that observed in the control. SBA-15-adjuvanted oral vaccine provided immunological response, decreased shedding of M. hyopneumoniae and led to mucosal protection confirmed by the reduced pulmonary lesions. This study provides useful data for future development of vaccines against M. hyopneumoniae. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T13:05:38Z 2023-07-29T13:05:38Z 2023-05-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.rvsc.2023.03.018 Research in Veterinary Science, v. 158, p. 141-150. 1532-2661 0034-5288 http://hdl.handle.net/11449/247078 10.1016/j.rvsc.2023.03.018 2-s2.0-85151252565 |
url |
http://dx.doi.org/10.1016/j.rvsc.2023.03.018 http://hdl.handle.net/11449/247078 |
identifier_str_mv |
Research in Veterinary Science, v. 158, p. 141-150. 1532-2661 0034-5288 10.1016/j.rvsc.2023.03.018 2-s2.0-85151252565 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Research in Veterinary Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
141-150 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129070776451072 |