Exploring the Potential of Two Bacterial Consortia to Degrade Cellulosic Biomass for Biotechnological Applications
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00284-020-02136-7 http://hdl.handle.net/11449/201976 |
Resumo: | This paper outlines two cellulolytic bacterial consortia named SCS and SCB, isolated from soil samples of sugarcane (Saccharum officinarum) crop field, and a sugarcane bagasse deposit in an ethanol mill. Both consortia were able to grow on different carbon sources, such as sugarcane bagasse, corn husk, peanut hulls, and carboxymethylcellulose, releasing up to 11.90 µmol/mL and 15.23 µmol/mL of glucose for SCS and SCB, respectively. In addition, SCS and SCB have several strains capable of producing cellulase, amylase, lipase, and protease. Whole genome sequencing of the SCS consortium revealed that Burkholderia was the most prevalent genus, encompassing approximately 80% of the consortia. In addition, metagenome analysis allowed the identification of genes encoding enzymes related to starch and cellulose degradation, as well as enzymes related to lipases and proteases, confirming our initial findings. The results showed that SCS and SCB had the capability to degrade cellulose, and that they were an efficient source of enzyme production, which would provide a new choice for use in different biotechnological applications. |
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Repositório Institucional da UNESP |
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spelling |
Exploring the Potential of Two Bacterial Consortia to Degrade Cellulosic Biomass for Biotechnological ApplicationsThis paper outlines two cellulolytic bacterial consortia named SCS and SCB, isolated from soil samples of sugarcane (Saccharum officinarum) crop field, and a sugarcane bagasse deposit in an ethanol mill. Both consortia were able to grow on different carbon sources, such as sugarcane bagasse, corn husk, peanut hulls, and carboxymethylcellulose, releasing up to 11.90 µmol/mL and 15.23 µmol/mL of glucose for SCS and SCB, respectively. In addition, SCS and SCB have several strains capable of producing cellulase, amylase, lipase, and protease. Whole genome sequencing of the SCS consortium revealed that Burkholderia was the most prevalent genus, encompassing approximately 80% of the consortia. In addition, metagenome analysis allowed the identification of genes encoding enzymes related to starch and cellulose degradation, as well as enzymes related to lipases and proteases, confirming our initial findings. The results showed that SCS and SCB had the capability to degrade cellulose, and that they were an efficient source of enzyme production, which would provide a new choice for use in different biotechnological applications.Department of Technology São Paulo State University (UNESP) School of Agricultural and Veterinarian Sciences, Via de Acesso Prof. Paulo Donato Castellane, s/nDepartment of Technology São Paulo State University (UNESP) School of Agricultural and Veterinarian Sciences, Via de Acesso Prof. Paulo Donato Castellane, s/nUniversidade Estadual Paulista (Unesp)Constancio, Milena Tavares Lima [UNESP]Sacco, Laís Postai [UNESP]Campanharo, João Carlos [UNESP]Castellane, Tereza Cristina Luque [UNESP]de Oliveira Souza, Anna Carolina [UNESP]Weiss, Bruno [UNESP]de Mello Varani, Alessandro [UNESP]Alves, Lúcia Maria Carareto [UNESP]2020-12-12T02:46:39Z2020-12-12T02:46:39Z2020-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article3114-3124http://dx.doi.org/10.1007/s00284-020-02136-7Current Microbiology, v. 77, n. 10, p. 3114-3124, 2020.1432-09910343-8651http://hdl.handle.net/11449/20197610.1007/s00284-020-02136-72-s2.0-85088651124Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCurrent Microbiologyinfo:eu-repo/semantics/openAccess2021-10-23T04:15:50Zoai:repositorio.unesp.br:11449/201976Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T04:15:50Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Exploring the Potential of Two Bacterial Consortia to Degrade Cellulosic Biomass for Biotechnological Applications |
title |
Exploring the Potential of Two Bacterial Consortia to Degrade Cellulosic Biomass for Biotechnological Applications |
spellingShingle |
Exploring the Potential of Two Bacterial Consortia to Degrade Cellulosic Biomass for Biotechnological Applications Constancio, Milena Tavares Lima [UNESP] |
title_short |
Exploring the Potential of Two Bacterial Consortia to Degrade Cellulosic Biomass for Biotechnological Applications |
title_full |
Exploring the Potential of Two Bacterial Consortia to Degrade Cellulosic Biomass for Biotechnological Applications |
title_fullStr |
Exploring the Potential of Two Bacterial Consortia to Degrade Cellulosic Biomass for Biotechnological Applications |
title_full_unstemmed |
Exploring the Potential of Two Bacterial Consortia to Degrade Cellulosic Biomass for Biotechnological Applications |
title_sort |
Exploring the Potential of Two Bacterial Consortia to Degrade Cellulosic Biomass for Biotechnological Applications |
author |
Constancio, Milena Tavares Lima [UNESP] |
author_facet |
Constancio, Milena Tavares Lima [UNESP] Sacco, Laís Postai [UNESP] Campanharo, João Carlos [UNESP] Castellane, Tereza Cristina Luque [UNESP] de Oliveira Souza, Anna Carolina [UNESP] Weiss, Bruno [UNESP] de Mello Varani, Alessandro [UNESP] Alves, Lúcia Maria Carareto [UNESP] |
author_role |
author |
author2 |
Sacco, Laís Postai [UNESP] Campanharo, João Carlos [UNESP] Castellane, Tereza Cristina Luque [UNESP] de Oliveira Souza, Anna Carolina [UNESP] Weiss, Bruno [UNESP] de Mello Varani, Alessandro [UNESP] Alves, Lúcia Maria Carareto [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Constancio, Milena Tavares Lima [UNESP] Sacco, Laís Postai [UNESP] Campanharo, João Carlos [UNESP] Castellane, Tereza Cristina Luque [UNESP] de Oliveira Souza, Anna Carolina [UNESP] Weiss, Bruno [UNESP] de Mello Varani, Alessandro [UNESP] Alves, Lúcia Maria Carareto [UNESP] |
description |
This paper outlines two cellulolytic bacterial consortia named SCS and SCB, isolated from soil samples of sugarcane (Saccharum officinarum) crop field, and a sugarcane bagasse deposit in an ethanol mill. Both consortia were able to grow on different carbon sources, such as sugarcane bagasse, corn husk, peanut hulls, and carboxymethylcellulose, releasing up to 11.90 µmol/mL and 15.23 µmol/mL of glucose for SCS and SCB, respectively. In addition, SCS and SCB have several strains capable of producing cellulase, amylase, lipase, and protease. Whole genome sequencing of the SCS consortium revealed that Burkholderia was the most prevalent genus, encompassing approximately 80% of the consortia. In addition, metagenome analysis allowed the identification of genes encoding enzymes related to starch and cellulose degradation, as well as enzymes related to lipases and proteases, confirming our initial findings. The results showed that SCS and SCB had the capability to degrade cellulose, and that they were an efficient source of enzyme production, which would provide a new choice for use in different biotechnological applications. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:46:39Z 2020-12-12T02:46:39Z 2020-10-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00284-020-02136-7 Current Microbiology, v. 77, n. 10, p. 3114-3124, 2020. 1432-0991 0343-8651 http://hdl.handle.net/11449/201976 10.1007/s00284-020-02136-7 2-s2.0-85088651124 |
url |
http://dx.doi.org/10.1007/s00284-020-02136-7 http://hdl.handle.net/11449/201976 |
identifier_str_mv |
Current Microbiology, v. 77, n. 10, p. 3114-3124, 2020. 1432-0991 0343-8651 10.1007/s00284-020-02136-7 2-s2.0-85088651124 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Current Microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
3114-3124 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964928101056512 |