Pharmacological evidence that methylene blue inhibits noradrenaline neuronal uptake in the rat vas deferens
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/230951 |
Resumo: | We report that the classical guanylate cyclase inhibitor methylene blue (MB, 1 μM or 10 μM), but not the selective guanylate cyclase inhibitor 1H-[1,2,4]oxidazolo[4,3-a]quinoxalin-1-one (1 μM) or nitric oxide synthase inhibitor, NG-nitro-L-arginine methyl ester (100 μM), causes a shift to the left in the concentration-response curve for noradrenaline in the isolated rat vas deferens preparations. The main objective of our study was to investigate the pharmacological mechanism by which MB increases the sensitivity of the rat vas deferens to noradrenaline. According to the presented results, MB did not change rat vas deferens sensitivity to methoxamine or noradrenaline in the presence of desipramine (0.1 μM). The pre-contracted rat vas deferens relaxation induced by isoproterenol was also not significantly changed by MB (1 μM). Thus, we suggest that MB increases rat vas deferens sensitivity through neuronal uptake inhibition without interfering in either the nitrergic mechanism or guanylate cyclase activity. |
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Pharmacological evidence that methylene blue inhibits noradrenaline neuronal uptake in the rat vas deferensGuanylate cyclaseMethylene blueNeuronal uptakeNoradrenalineRat vas deferensWe report that the classical guanylate cyclase inhibitor methylene blue (MB, 1 μM or 10 μM), but not the selective guanylate cyclase inhibitor 1H-[1,2,4]oxidazolo[4,3-a]quinoxalin-1-one (1 μM) or nitric oxide synthase inhibitor, NG-nitro-L-arginine methyl ester (100 μM), causes a shift to the left in the concentration-response curve for noradrenaline in the isolated rat vas deferens preparations. The main objective of our study was to investigate the pharmacological mechanism by which MB increases the sensitivity of the rat vas deferens to noradrenaline. According to the presented results, MB did not change rat vas deferens sensitivity to methoxamine or noradrenaline in the presence of desipramine (0.1 μM). The pre-contracted rat vas deferens relaxation induced by isoproterenol was also not significantly changed by MB (1 μM). Thus, we suggest that MB increases rat vas deferens sensitivity through neuronal uptake inhibition without interfering in either the nitrergic mechanism or guanylate cyclase activity.Laboratory of Pharmacology School of Medicine of Marília, Marília 17519-030 SPDepartment of Pharmacology School of Medicine of Ribeirao Preto, Ribeirão Preto 14049-900 SPDepartment of Pharmacology Biosciences Institute of Botucatu, Botucatu 18.618-000 SPSchool of Medicine of MaríliaSchool of Medicine of Ribeirao PretoBiosciences Institute of BotucatuChies, Agnaldo B.Custódio, Ricardo C.de Souza, GregórioCorrêa, Fernando M.A.Pereira, Oduvaldo C.M.2022-04-29T08:42:51Z2022-04-29T08:42:51Z2003-10-09info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article573-579Polish Journal of Pharmacology, v. 55, n. 4, p. 573-579, 2003.1230-6002http://hdl.handle.net/11449/2309512-s2.0-0141725416Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPolish Journal of Pharmacologyinfo:eu-repo/semantics/openAccess2022-04-29T08:42:52Zoai:repositorio.unesp.br:11449/230951Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:20:14.504615Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Pharmacological evidence that methylene blue inhibits noradrenaline neuronal uptake in the rat vas deferens |
title |
Pharmacological evidence that methylene blue inhibits noradrenaline neuronal uptake in the rat vas deferens |
spellingShingle |
Pharmacological evidence that methylene blue inhibits noradrenaline neuronal uptake in the rat vas deferens Chies, Agnaldo B. Guanylate cyclase Methylene blue Neuronal uptake Noradrenaline Rat vas deferens |
title_short |
Pharmacological evidence that methylene blue inhibits noradrenaline neuronal uptake in the rat vas deferens |
title_full |
Pharmacological evidence that methylene blue inhibits noradrenaline neuronal uptake in the rat vas deferens |
title_fullStr |
Pharmacological evidence that methylene blue inhibits noradrenaline neuronal uptake in the rat vas deferens |
title_full_unstemmed |
Pharmacological evidence that methylene blue inhibits noradrenaline neuronal uptake in the rat vas deferens |
title_sort |
Pharmacological evidence that methylene blue inhibits noradrenaline neuronal uptake in the rat vas deferens |
author |
Chies, Agnaldo B. |
author_facet |
Chies, Agnaldo B. Custódio, Ricardo C. de Souza, Gregório Corrêa, Fernando M.A. Pereira, Oduvaldo C.M. |
author_role |
author |
author2 |
Custódio, Ricardo C. de Souza, Gregório Corrêa, Fernando M.A. Pereira, Oduvaldo C.M. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
School of Medicine of Marília School of Medicine of Ribeirao Preto Biosciences Institute of Botucatu |
dc.contributor.author.fl_str_mv |
Chies, Agnaldo B. Custódio, Ricardo C. de Souza, Gregório Corrêa, Fernando M.A. Pereira, Oduvaldo C.M. |
dc.subject.por.fl_str_mv |
Guanylate cyclase Methylene blue Neuronal uptake Noradrenaline Rat vas deferens |
topic |
Guanylate cyclase Methylene blue Neuronal uptake Noradrenaline Rat vas deferens |
description |
We report that the classical guanylate cyclase inhibitor methylene blue (MB, 1 μM or 10 μM), but not the selective guanylate cyclase inhibitor 1H-[1,2,4]oxidazolo[4,3-a]quinoxalin-1-one (1 μM) or nitric oxide synthase inhibitor, NG-nitro-L-arginine methyl ester (100 μM), causes a shift to the left in the concentration-response curve for noradrenaline in the isolated rat vas deferens preparations. The main objective of our study was to investigate the pharmacological mechanism by which MB increases the sensitivity of the rat vas deferens to noradrenaline. According to the presented results, MB did not change rat vas deferens sensitivity to methoxamine or noradrenaline in the presence of desipramine (0.1 μM). The pre-contracted rat vas deferens relaxation induced by isoproterenol was also not significantly changed by MB (1 μM). Thus, we suggest that MB increases rat vas deferens sensitivity through neuronal uptake inhibition without interfering in either the nitrergic mechanism or guanylate cyclase activity. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003-10-09 2022-04-29T08:42:51Z 2022-04-29T08:42:51Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Polish Journal of Pharmacology, v. 55, n. 4, p. 573-579, 2003. 1230-6002 http://hdl.handle.net/11449/230951 2-s2.0-0141725416 |
identifier_str_mv |
Polish Journal of Pharmacology, v. 55, n. 4, p. 573-579, 2003. 1230-6002 2-s2.0-0141725416 |
url |
http://hdl.handle.net/11449/230951 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Polish Journal of Pharmacology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
573-579 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128793374621696 |