Effect of the Association of Nonsteroidal Anti-inflammatory and Antibiotic Drugs on Antibiofilm Activity and pH of Calcium Hydroxide Pastes
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.joen.2016.09.014 http://hdl.handle.net/11449/162306 |
Resumo: | Introduction: The objective of the present study was to evaluate the in vitro antibiofilm activity and pH of calcium hydroxide associated with different nonsteroidal anti-inflammatory drugs (NSAIDs). Methods: The groups analyzed were as follows: group 1, calcium hydroxide paste with propylene glycol; group 2, calcium hydroxide paste with propylene glycol + 5% diclofenac sodium; group 3, calcium hydroxide paste with propylene glycol + 5% ibuprofen; group 4, calcium hydroxide paste with propylene glycol + 5% ciprofloxacin; and group 6, positive control (without medication). For analysis of the pH, the pastes were inserted into tubes and immersed in flasks containing ultrapure water. At the time intervals of 3, 24, 72, and 168 hours, the pH was measured with a calibrated pH meter. For microbial analysis, biofilm was induced in 30 bovine dentin blocks for 21 days. Subsequently, the pastes were placed on the blocks with biofilm for 7 days. Afterward, the pastes were removed by irrigation with sterile water, and the specimens were analyzed with a laser scanning confocal microscope with the 50 mu L Live/Dead BacLight Bacterial Viability solution L7012 Kit (Molecular Probes, Inc, Eugene, OR). Data were subjected to statistical analysis at a significance level of 5%. Results: The highest pH values were found for calcium hydroxide associated with ciprofloxacin in all periods analyzed. With the exception of pure calcium hydroxide paste, the other groups showed statistically significant differences (P <.05) in comparison with the positive control. Conclusions: The association of NSAIDs or antibiotic did not interfere with the pH of calcium hydroxide paste and increased the antimicrobial action of calcium hydroxide paste against Enterococcus faecalis biofilm formation. |
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Effect of the Association of Nonsteroidal Anti-inflammatory and Antibiotic Drugs on Antibiofilm Activity and pH of Calcium Hydroxide PastesAntibiofilm actioncalcium hydroxidediclofenacibuprofenintracanal medicationIntroduction: The objective of the present study was to evaluate the in vitro antibiofilm activity and pH of calcium hydroxide associated with different nonsteroidal anti-inflammatory drugs (NSAIDs). Methods: The groups analyzed were as follows: group 1, calcium hydroxide paste with propylene glycol; group 2, calcium hydroxide paste with propylene glycol + 5% diclofenac sodium; group 3, calcium hydroxide paste with propylene glycol + 5% ibuprofen; group 4, calcium hydroxide paste with propylene glycol + 5% ciprofloxacin; and group 6, positive control (without medication). For analysis of the pH, the pastes were inserted into tubes and immersed in flasks containing ultrapure water. At the time intervals of 3, 24, 72, and 168 hours, the pH was measured with a calibrated pH meter. For microbial analysis, biofilm was induced in 30 bovine dentin blocks for 21 days. Subsequently, the pastes were placed on the blocks with biofilm for 7 days. Afterward, the pastes were removed by irrigation with sterile water, and the specimens were analyzed with a laser scanning confocal microscope with the 50 mu L Live/Dead BacLight Bacterial Viability solution L7012 Kit (Molecular Probes, Inc, Eugene, OR). Data were subjected to statistical analysis at a significance level of 5%. Results: The highest pH values were found for calcium hydroxide associated with ciprofloxacin in all periods analyzed. With the exception of pure calcium hydroxide paste, the other groups showed statistically significant differences (P <.05) in comparison with the positive control. Conclusions: The association of NSAIDs or antibiotic did not interfere with the pH of calcium hydroxide paste and increased the antimicrobial action of calcium hydroxide paste against Enterococcus faecalis biofilm formation.Univ Sagrado Coracao, Prorectory Res & Post Graduat, Bauru, SP, BrazilSao Paulo State Univ, Sch Pharmaceut Sci, Dept Drugs & Med, Pharmaceut Biotechnol Lab, Araraquara, SP, BrazilUniv Sao Paulo, Bauru Sch Dent, Dept Operat Dent Endodont & Dent Mat, Bauru, SP, BrazilUniv Fed Parana, Dept Restorat Dent, Curitiba, Parana, BrazilLauro De Souza Lima Inst, Micol Dept, Bauru, SP, BrazilSao Paulo State Univ, Sch Pharmaceut Sci, Dept Drugs & Med, Pharmaceut Biotechnol Lab, Araraquara, SP, BrazilElsevier B.V.Univ Sagrado CoracaoUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Univ Fed ParanaLauro De Souza Lima InstFreitas, Rafaela Pignatti deGreatti, Vanessa Raquel [UNESP]Alcalde, Murilo PrioriCavenago, Bruno CavaliniVivan, Rodrigo RicciHungaro Duarte, Marco AntonioVillas Boas Weckwerth, Ana CarolinaWeckwerth, Paulo Henrique2018-11-26T17:15:33Z2018-11-26T17:15:33Z2017-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article131-134application/pdfhttp://dx.doi.org/10.1016/j.joen.2016.09.014Journal Of Endodontics. New York: Elsevier Science Inc, v. 43, n. 1, p. 131-134, 2017.0099-2399http://hdl.handle.net/11449/16230610.1016/j.joen.2016.09.014WOS:000391246400020WOS000391246400020.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Endodontics1,585info:eu-repo/semantics/openAccess2024-06-24T13:45:30Zoai:repositorio.unesp.br:11449/162306Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:00:25.272610Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effect of the Association of Nonsteroidal Anti-inflammatory and Antibiotic Drugs on Antibiofilm Activity and pH of Calcium Hydroxide Pastes |
title |
Effect of the Association of Nonsteroidal Anti-inflammatory and Antibiotic Drugs on Antibiofilm Activity and pH of Calcium Hydroxide Pastes |
spellingShingle |
Effect of the Association of Nonsteroidal Anti-inflammatory and Antibiotic Drugs on Antibiofilm Activity and pH of Calcium Hydroxide Pastes Freitas, Rafaela Pignatti de Antibiofilm action calcium hydroxide diclofenac ibuprofen intracanal medication |
title_short |
Effect of the Association of Nonsteroidal Anti-inflammatory and Antibiotic Drugs on Antibiofilm Activity and pH of Calcium Hydroxide Pastes |
title_full |
Effect of the Association of Nonsteroidal Anti-inflammatory and Antibiotic Drugs on Antibiofilm Activity and pH of Calcium Hydroxide Pastes |
title_fullStr |
Effect of the Association of Nonsteroidal Anti-inflammatory and Antibiotic Drugs on Antibiofilm Activity and pH of Calcium Hydroxide Pastes |
title_full_unstemmed |
Effect of the Association of Nonsteroidal Anti-inflammatory and Antibiotic Drugs on Antibiofilm Activity and pH of Calcium Hydroxide Pastes |
title_sort |
Effect of the Association of Nonsteroidal Anti-inflammatory and Antibiotic Drugs on Antibiofilm Activity and pH of Calcium Hydroxide Pastes |
author |
Freitas, Rafaela Pignatti de |
author_facet |
Freitas, Rafaela Pignatti de Greatti, Vanessa Raquel [UNESP] Alcalde, Murilo Priori Cavenago, Bruno Cavalini Vivan, Rodrigo Ricci Hungaro Duarte, Marco Antonio Villas Boas Weckwerth, Ana Carolina Weckwerth, Paulo Henrique |
author_role |
author |
author2 |
Greatti, Vanessa Raquel [UNESP] Alcalde, Murilo Priori Cavenago, Bruno Cavalini Vivan, Rodrigo Ricci Hungaro Duarte, Marco Antonio Villas Boas Weckwerth, Ana Carolina Weckwerth, Paulo Henrique |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Univ Sagrado Coracao Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) Univ Fed Parana Lauro De Souza Lima Inst |
dc.contributor.author.fl_str_mv |
Freitas, Rafaela Pignatti de Greatti, Vanessa Raquel [UNESP] Alcalde, Murilo Priori Cavenago, Bruno Cavalini Vivan, Rodrigo Ricci Hungaro Duarte, Marco Antonio Villas Boas Weckwerth, Ana Carolina Weckwerth, Paulo Henrique |
dc.subject.por.fl_str_mv |
Antibiofilm action calcium hydroxide diclofenac ibuprofen intracanal medication |
topic |
Antibiofilm action calcium hydroxide diclofenac ibuprofen intracanal medication |
description |
Introduction: The objective of the present study was to evaluate the in vitro antibiofilm activity and pH of calcium hydroxide associated with different nonsteroidal anti-inflammatory drugs (NSAIDs). Methods: The groups analyzed were as follows: group 1, calcium hydroxide paste with propylene glycol; group 2, calcium hydroxide paste with propylene glycol + 5% diclofenac sodium; group 3, calcium hydroxide paste with propylene glycol + 5% ibuprofen; group 4, calcium hydroxide paste with propylene glycol + 5% ciprofloxacin; and group 6, positive control (without medication). For analysis of the pH, the pastes were inserted into tubes and immersed in flasks containing ultrapure water. At the time intervals of 3, 24, 72, and 168 hours, the pH was measured with a calibrated pH meter. For microbial analysis, biofilm was induced in 30 bovine dentin blocks for 21 days. Subsequently, the pastes were placed on the blocks with biofilm for 7 days. Afterward, the pastes were removed by irrigation with sterile water, and the specimens were analyzed with a laser scanning confocal microscope with the 50 mu L Live/Dead BacLight Bacterial Viability solution L7012 Kit (Molecular Probes, Inc, Eugene, OR). Data were subjected to statistical analysis at a significance level of 5%. Results: The highest pH values were found for calcium hydroxide associated with ciprofloxacin in all periods analyzed. With the exception of pure calcium hydroxide paste, the other groups showed statistically significant differences (P <.05) in comparison with the positive control. Conclusions: The association of NSAIDs or antibiotic did not interfere with the pH of calcium hydroxide paste and increased the antimicrobial action of calcium hydroxide paste against Enterococcus faecalis biofilm formation. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-01 2018-11-26T17:15:33Z 2018-11-26T17:15:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.joen.2016.09.014 Journal Of Endodontics. New York: Elsevier Science Inc, v. 43, n. 1, p. 131-134, 2017. 0099-2399 http://hdl.handle.net/11449/162306 10.1016/j.joen.2016.09.014 WOS:000391246400020 WOS000391246400020.pdf |
url |
http://dx.doi.org/10.1016/j.joen.2016.09.014 http://hdl.handle.net/11449/162306 |
identifier_str_mv |
Journal Of Endodontics. New York: Elsevier Science Inc, v. 43, n. 1, p. 131-134, 2017. 0099-2399 10.1016/j.joen.2016.09.014 WOS:000391246400020 WOS000391246400020.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Endodontics 1,585 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
131-134 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128737439383552 |