The Effect of Toothpastes Containing Natural Extracts on Bacterial Species of a Microcosm Biofilm and on Enamel Caries Development

Detalhes bibliográficos
Autor(a) principal: Braga, Aline Silva
Data de Publicação: 2022
Outros Autores: Abdelbary, Mohamed Mostafa Hefny, Kim, Rafaela Ricci, de Melo, Fernanda Pereira de Souza Rosa [UNESP], Saldanha, Luiz Leonardo [UNESP], Dokkedal, Anne Lígia [UNESP], Conrads, Georg, Esteves-Oliveira, Marcella, Magalhães, Ana Carolina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3390/antibiotics11030414
http://hdl.handle.net/11449/231649
Resumo: This study investigated the effects of herbal toothpaste on bacterial counts and enamel demineralization. Thirty-six bovine enamel samples were exposed to a microcosm biofilm using human saliva and McBain saliva (0.2% sucrose) for 5 days at 37 °C and first incubated anaerobically, then aerobically–capnophilically. The following experimental toothpaste slurries (2 × 2 min/day) were applied: (1) Vochysia tucanorum (10 mg/g); (2) Myrcia bella (5 mg/g); (3) Matricaria chamomilla (80 mg/g); (4) Myrrha and propolis toothpaste (commercial); (5) fluoride (F) and triclosan (1450 ppm F), 0.3% triclosan and sorbitol (Colgate®, positive control); (6) placebo (negative control). The pH of the medium was measured, bacteria were analyzed using quantitative polymerase chain reaction, and enamel demineralization was quantified using transverse microradiography. The total bacterial count was reduced by toothpaste containing Myrcia bella, Matricaria chamomilla, fluoride, and triclosan (commercial) compared to the placebo. As far as assessable, Myrcia bella, Matricaria chamomilla, and Myrrha and propolis (commercial) inhibited the outgrowth of S. mutans, while Lactobacillus spp. were reduced/eliminated by all toothpastes except Vochysia tucanorum. Mineral loss and lesion depth were significantly reduced by all toothpastes (total: 1423.6 ± 115.2 vol% × μm; 57.3 ± 9.8 μm) compared to the placebo (2420.0 ± 626.0 vol% × μm; 108.9 ± 21.17 μm). Herbal toothpastes were able to reduce enamel demineralization.
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spelling The Effect of Toothpastes Containing Natural Extracts on Bacterial Species of a Microcosm Biofilm and on Enamel Caries Developmentantimicrobial strategiesdental cariesoral biofilmsplantsThis study investigated the effects of herbal toothpaste on bacterial counts and enamel demineralization. Thirty-six bovine enamel samples were exposed to a microcosm biofilm using human saliva and McBain saliva (0.2% sucrose) for 5 days at 37 °C and first incubated anaerobically, then aerobically–capnophilically. The following experimental toothpaste slurries (2 × 2 min/day) were applied: (1) Vochysia tucanorum (10 mg/g); (2) Myrcia bella (5 mg/g); (3) Matricaria chamomilla (80 mg/g); (4) Myrrha and propolis toothpaste (commercial); (5) fluoride (F) and triclosan (1450 ppm F), 0.3% triclosan and sorbitol (Colgate®, positive control); (6) placebo (negative control). The pH of the medium was measured, bacteria were analyzed using quantitative polymerase chain reaction, and enamel demineralization was quantified using transverse microradiography. The total bacterial count was reduced by toothpaste containing Myrcia bella, Matricaria chamomilla, fluoride, and triclosan (commercial) compared to the placebo. As far as assessable, Myrcia bella, Matricaria chamomilla, and Myrrha and propolis (commercial) inhibited the outgrowth of S. mutans, while Lactobacillus spp. were reduced/eliminated by all toothpastes except Vochysia tucanorum. Mineral loss and lesion depth were significantly reduced by all toothpastes (total: 1423.6 ± 115.2 vol% × μm; 57.3 ± 9.8 μm) compared to the placebo (2420.0 ± 626.0 vol% × μm; 108.9 ± 21.17 μm). Herbal toothpastes were able to reduce enamel demineralization.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Biological Sciences Bauru School of Dentistry University of São PauloDivision of Oral Microbiology and Immunology Department of Operative and Preventive Dentistry and Periodontology RWTH Aachen University HospitalDepartment of Biological Sciences School of Science The São Paulo State University (UNESP)Department of Restorative Preventive and Pediatric Dentistry University of BernDepartment of Biological Sciences School of Science The São Paulo State University (UNESP)FAPESP: 2017/00556-0FAPESP: 2017/17249-2FAPESP: 2018/26506-1FAPESP: 2019/21797-0Universidade de São Paulo (USP)RWTH Aachen University HospitalUniversidade Estadual Paulista (UNESP)University of BernBraga, Aline SilvaAbdelbary, Mohamed Mostafa HefnyKim, Rafaela Riccide Melo, Fernanda Pereira de Souza Rosa [UNESP]Saldanha, Luiz Leonardo [UNESP]Dokkedal, Anne Lígia [UNESP]Conrads, GeorgEsteves-Oliveira, MarcellaMagalhães, Ana Carolina2022-04-29T08:46:48Z2022-04-29T08:46:48Z2022-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/antibiotics11030414Antibiotics, v. 11, n. 3, 2022.2079-6382http://hdl.handle.net/11449/23164910.3390/antibiotics110304142-s2.0-85127660874Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAntibioticsinfo:eu-repo/semantics/openAccess2024-04-23T15:23:49Zoai:repositorio.unesp.br:11449/231649Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:44:46.634376Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv The Effect of Toothpastes Containing Natural Extracts on Bacterial Species of a Microcosm Biofilm and on Enamel Caries Development
title The Effect of Toothpastes Containing Natural Extracts on Bacterial Species of a Microcosm Biofilm and on Enamel Caries Development
spellingShingle The Effect of Toothpastes Containing Natural Extracts on Bacterial Species of a Microcosm Biofilm and on Enamel Caries Development
Braga, Aline Silva
antimicrobial strategies
dental caries
oral biofilms
plants
title_short The Effect of Toothpastes Containing Natural Extracts on Bacterial Species of a Microcosm Biofilm and on Enamel Caries Development
title_full The Effect of Toothpastes Containing Natural Extracts on Bacterial Species of a Microcosm Biofilm and on Enamel Caries Development
title_fullStr The Effect of Toothpastes Containing Natural Extracts on Bacterial Species of a Microcosm Biofilm and on Enamel Caries Development
title_full_unstemmed The Effect of Toothpastes Containing Natural Extracts on Bacterial Species of a Microcosm Biofilm and on Enamel Caries Development
title_sort The Effect of Toothpastes Containing Natural Extracts on Bacterial Species of a Microcosm Biofilm and on Enamel Caries Development
author Braga, Aline Silva
author_facet Braga, Aline Silva
Abdelbary, Mohamed Mostafa Hefny
Kim, Rafaela Ricci
de Melo, Fernanda Pereira de Souza Rosa [UNESP]
Saldanha, Luiz Leonardo [UNESP]
Dokkedal, Anne Lígia [UNESP]
Conrads, Georg
Esteves-Oliveira, Marcella
Magalhães, Ana Carolina
author_role author
author2 Abdelbary, Mohamed Mostafa Hefny
Kim, Rafaela Ricci
de Melo, Fernanda Pereira de Souza Rosa [UNESP]
Saldanha, Luiz Leonardo [UNESP]
Dokkedal, Anne Lígia [UNESP]
Conrads, Georg
Esteves-Oliveira, Marcella
Magalhães, Ana Carolina
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
RWTH Aachen University Hospital
Universidade Estadual Paulista (UNESP)
University of Bern
dc.contributor.author.fl_str_mv Braga, Aline Silva
Abdelbary, Mohamed Mostafa Hefny
Kim, Rafaela Ricci
de Melo, Fernanda Pereira de Souza Rosa [UNESP]
Saldanha, Luiz Leonardo [UNESP]
Dokkedal, Anne Lígia [UNESP]
Conrads, Georg
Esteves-Oliveira, Marcella
Magalhães, Ana Carolina
dc.subject.por.fl_str_mv antimicrobial strategies
dental caries
oral biofilms
plants
topic antimicrobial strategies
dental caries
oral biofilms
plants
description This study investigated the effects of herbal toothpaste on bacterial counts and enamel demineralization. Thirty-six bovine enamel samples were exposed to a microcosm biofilm using human saliva and McBain saliva (0.2% sucrose) for 5 days at 37 °C and first incubated anaerobically, then aerobically–capnophilically. The following experimental toothpaste slurries (2 × 2 min/day) were applied: (1) Vochysia tucanorum (10 mg/g); (2) Myrcia bella (5 mg/g); (3) Matricaria chamomilla (80 mg/g); (4) Myrrha and propolis toothpaste (commercial); (5) fluoride (F) and triclosan (1450 ppm F), 0.3% triclosan and sorbitol (Colgate®, positive control); (6) placebo (negative control). The pH of the medium was measured, bacteria were analyzed using quantitative polymerase chain reaction, and enamel demineralization was quantified using transverse microradiography. The total bacterial count was reduced by toothpaste containing Myrcia bella, Matricaria chamomilla, fluoride, and triclosan (commercial) compared to the placebo. As far as assessable, Myrcia bella, Matricaria chamomilla, and Myrrha and propolis (commercial) inhibited the outgrowth of S. mutans, while Lactobacillus spp. were reduced/eliminated by all toothpastes except Vochysia tucanorum. Mineral loss and lesion depth were significantly reduced by all toothpastes (total: 1423.6 ± 115.2 vol% × μm; 57.3 ± 9.8 μm) compared to the placebo (2420.0 ± 626.0 vol% × μm; 108.9 ± 21.17 μm). Herbal toothpastes were able to reduce enamel demineralization.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-29T08:46:48Z
2022-04-29T08:46:48Z
2022-03-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3390/antibiotics11030414
Antibiotics, v. 11, n. 3, 2022.
2079-6382
http://hdl.handle.net/11449/231649
10.3390/antibiotics11030414
2-s2.0-85127660874
url http://dx.doi.org/10.3390/antibiotics11030414
http://hdl.handle.net/11449/231649
identifier_str_mv Antibiotics, v. 11, n. 3, 2022.
2079-6382
10.3390/antibiotics11030414
2-s2.0-85127660874
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Antibiotics
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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