Deregulation of E-cadherin, β-catenin, APC and Caveolin-1 expression occurs in canine prostate cancer and metastatic processes
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.rvsc.2018.03.004 http://hdl.handle.net/11449/175995 |
Resumo: | Prostate cancer is a heterogeneous disease with high levels of clinical and gene heterogeneity, consequently offering several targets for therapy. Dogs with naturally occurring prostate cancer are useful models for molecular investigations and studying new treatment efficacy. Three genes and proteins associated with the WNT pathway (β-catenin, APC and E-cadherin) and Caveolin-1 (CAV-1) were evaluated in canine pre-neoplastic proliferative inflammatory atrophy (PIA), prostate cancer and metastatic disease. The APC gene methylation status was also investigated. As in human prostate cancer, cytoplasmic and nuclear β-catenin, which are fundamental for activating the canonical WNT pathway, were found in canine prostate cancer and metastasis. Membranous E-cadherin was also lost in these lesions, allowing cellular migration to the stroma and nuclear localization of β-catenin. In contrast to human prostate tumours, no APC downregulation or hypermethylation was found in canine prostate cancer. The CAV-1 gene and protein overexpression were found in canine prostate cancer, and as in humans, the highest levels were found in Gleason scores ≥8. In conclusion, as with human prostate cancer, β-catenin and E-cadherin in the WNT pathway, as well as Caveolin-1, are molecular drivers in canine prostate cancer. These findings provide additional evidence that dogs are useful models for studying new therapeutic targets in prostate cancer. |
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Deregulation of E-cadherin, β-catenin, APC and Caveolin-1 expression occurs in canine prostate cancer and metastatic processesDogMetastasisOncologyProstatic carcinomaWNT pathwayProstate cancer is a heterogeneous disease with high levels of clinical and gene heterogeneity, consequently offering several targets for therapy. Dogs with naturally occurring prostate cancer are useful models for molecular investigations and studying new treatment efficacy. Three genes and proteins associated with the WNT pathway (β-catenin, APC and E-cadherin) and Caveolin-1 (CAV-1) were evaluated in canine pre-neoplastic proliferative inflammatory atrophy (PIA), prostate cancer and metastatic disease. The APC gene methylation status was also investigated. As in human prostate cancer, cytoplasmic and nuclear β-catenin, which are fundamental for activating the canonical WNT pathway, were found in canine prostate cancer and metastasis. Membranous E-cadherin was also lost in these lesions, allowing cellular migration to the stroma and nuclear localization of β-catenin. In contrast to human prostate tumours, no APC downregulation or hypermethylation was found in canine prostate cancer. The CAV-1 gene and protein overexpression were found in canine prostate cancer, and as in humans, the highest levels were found in Gleason scores ≥8. In conclusion, as with human prostate cancer, β-catenin and E-cadherin in the WNT pathway, as well as Caveolin-1, are molecular drivers in canine prostate cancer. These findings provide additional evidence that dogs are useful models for studying new therapeutic targets in prostate cancer.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)São Paulo State University (UNESP) Department of Veterinary Clinic School of Veterinary Medicine and Animal ScienceSão Paulo State University (UNESP) Department of Veterinary Pathology School of Agricultural and Veterinarian SciencesInternational Center for Research (CIPE) AC Camargo Hospital, LiberdadeDepartment of Clinical Genetics Vejle Hospital and Institute of Regional Health University of Southern DenmarkSão Paulo State University (UNESP) Department of Veterinary Clinic School of Veterinary Medicine and Animal ScienceSão Paulo State University (UNESP) Department of Veterinary Pathology School of Agricultural and Veterinarian SciencesCNPq: 131323/2014-8FAPESP: 2012/16068-0CNPq: 443884/2014-5Universidade Estadual Paulista (Unesp)AC Camargo HospitalUniversity of Southern DenmarkKobayashi, Priscila E. [UNESP]Fonseca-Alves, Carlos E. [UNESP]Rivera-Calderón, Luis G. [UNESP]Carvalho, Márcio [UNESP]Kuasne, HellenRogatto, Silvia R.Laufer-Amorim, Renée [UNESP]2018-12-11T17:18:27Z2018-12-11T17:18:27Z2018-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article254-261application/pdfhttp://dx.doi.org/10.1016/j.rvsc.2018.03.004Research in Veterinary Science, v. 118, p. 254-261.1532-26610034-5288http://hdl.handle.net/11449/17599510.1016/j.rvsc.2018.03.0042-s2.0-850433760212-s2.0-85043376021.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengResearch in Veterinary Science0,593info:eu-repo/semantics/openAccess2023-11-27T06:16:13Zoai:repositorio.unesp.br:11449/175995Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:53:07.364680Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Deregulation of E-cadherin, β-catenin, APC and Caveolin-1 expression occurs in canine prostate cancer and metastatic processes |
title |
Deregulation of E-cadherin, β-catenin, APC and Caveolin-1 expression occurs in canine prostate cancer and metastatic processes |
spellingShingle |
Deregulation of E-cadherin, β-catenin, APC and Caveolin-1 expression occurs in canine prostate cancer and metastatic processes Kobayashi, Priscila E. [UNESP] Dog Metastasis Oncology Prostatic carcinoma WNT pathway |
title_short |
Deregulation of E-cadherin, β-catenin, APC and Caveolin-1 expression occurs in canine prostate cancer and metastatic processes |
title_full |
Deregulation of E-cadherin, β-catenin, APC and Caveolin-1 expression occurs in canine prostate cancer and metastatic processes |
title_fullStr |
Deregulation of E-cadherin, β-catenin, APC and Caveolin-1 expression occurs in canine prostate cancer and metastatic processes |
title_full_unstemmed |
Deregulation of E-cadherin, β-catenin, APC and Caveolin-1 expression occurs in canine prostate cancer and metastatic processes |
title_sort |
Deregulation of E-cadherin, β-catenin, APC and Caveolin-1 expression occurs in canine prostate cancer and metastatic processes |
author |
Kobayashi, Priscila E. [UNESP] |
author_facet |
Kobayashi, Priscila E. [UNESP] Fonseca-Alves, Carlos E. [UNESP] Rivera-Calderón, Luis G. [UNESP] Carvalho, Márcio [UNESP] Kuasne, Hellen Rogatto, Silvia R. Laufer-Amorim, Renée [UNESP] |
author_role |
author |
author2 |
Fonseca-Alves, Carlos E. [UNESP] Rivera-Calderón, Luis G. [UNESP] Carvalho, Márcio [UNESP] Kuasne, Hellen Rogatto, Silvia R. Laufer-Amorim, Renée [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) AC Camargo Hospital University of Southern Denmark |
dc.contributor.author.fl_str_mv |
Kobayashi, Priscila E. [UNESP] Fonseca-Alves, Carlos E. [UNESP] Rivera-Calderón, Luis G. [UNESP] Carvalho, Márcio [UNESP] Kuasne, Hellen Rogatto, Silvia R. Laufer-Amorim, Renée [UNESP] |
dc.subject.por.fl_str_mv |
Dog Metastasis Oncology Prostatic carcinoma WNT pathway |
topic |
Dog Metastasis Oncology Prostatic carcinoma WNT pathway |
description |
Prostate cancer is a heterogeneous disease with high levels of clinical and gene heterogeneity, consequently offering several targets for therapy. Dogs with naturally occurring prostate cancer are useful models for molecular investigations and studying new treatment efficacy. Three genes and proteins associated with the WNT pathway (β-catenin, APC and E-cadherin) and Caveolin-1 (CAV-1) were evaluated in canine pre-neoplastic proliferative inflammatory atrophy (PIA), prostate cancer and metastatic disease. The APC gene methylation status was also investigated. As in human prostate cancer, cytoplasmic and nuclear β-catenin, which are fundamental for activating the canonical WNT pathway, were found in canine prostate cancer and metastasis. Membranous E-cadherin was also lost in these lesions, allowing cellular migration to the stroma and nuclear localization of β-catenin. In contrast to human prostate tumours, no APC downregulation or hypermethylation was found in canine prostate cancer. The CAV-1 gene and protein overexpression were found in canine prostate cancer, and as in humans, the highest levels were found in Gleason scores ≥8. In conclusion, as with human prostate cancer, β-catenin and E-cadherin in the WNT pathway, as well as Caveolin-1, are molecular drivers in canine prostate cancer. These findings provide additional evidence that dogs are useful models for studying new therapeutic targets in prostate cancer. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:18:27Z 2018-12-11T17:18:27Z 2018-06-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.rvsc.2018.03.004 Research in Veterinary Science, v. 118, p. 254-261. 1532-2661 0034-5288 http://hdl.handle.net/11449/175995 10.1016/j.rvsc.2018.03.004 2-s2.0-85043376021 2-s2.0-85043376021.pdf |
url |
http://dx.doi.org/10.1016/j.rvsc.2018.03.004 http://hdl.handle.net/11449/175995 |
identifier_str_mv |
Research in Veterinary Science, v. 118, p. 254-261. 1532-2661 0034-5288 10.1016/j.rvsc.2018.03.004 2-s2.0-85043376021 2-s2.0-85043376021.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Research in Veterinary Science 0,593 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
254-261 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128994606841856 |