Heteromorphic Sex Chromosomes and Their DNA Content in Fish: An Insight through Satellite DNA Accumulation in Megaleporinus elongatus
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1159/000506265 http://hdl.handle.net/11449/196790 |
Resumo: | The repetitive DNA content of fish sex chromosomes provides valuable insights into specificities and patterns of their genetic sex determination systems. In this study, we revealed the genomic satellite DNA (satDNA) content of Megaleporinuselongatus, a Neotropical fish species with Z(1)Z(1)Z(2)Z(2)/Z(1)W(1)Z(2)W(2) multiple sex chromosomes, through high-throughput analysis and graph-based clustering, isolating 68 satDNA families. By physically mapping these sequences in female metaphases, we discovered 15 of the most abundant satDNAs clustered in its chromosomes, 9 of which were found exclusively in the highly heterochromatic W-1. This heteromorphic sex chromosome showed the highest amount of satDNA accumulations in this species. The second most abundant family, MelSat02-26, shared FISH signals with the NOR-bearing pair in similar patterns and is linked to the multiple sex chromosome system. Our results demonstrate the diverse satDNA content in M. elongatus, especially in its heteromorphic sex chromosome. Additionally, we highlighted the different accumulation patterns and distribution of these sequences across species by physically mapping these satDNAs in other Anostomidae, Megaleporinusmacrocephalus and Leporinusfriderici (a species without differentiated sex chromosomes). |
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Heteromorphic Sex Chromosomes and Their DNA Content in Fish: An Insight through Satellite DNA Accumulation in Megaleporinus elongatusFISHHeteromorphic sex chromosome systemNeotropical fishSatellitomeSex chromosome evolutionThe repetitive DNA content of fish sex chromosomes provides valuable insights into specificities and patterns of their genetic sex determination systems. In this study, we revealed the genomic satellite DNA (satDNA) content of Megaleporinuselongatus, a Neotropical fish species with Z(1)Z(1)Z(2)Z(2)/Z(1)W(1)Z(2)W(2) multiple sex chromosomes, through high-throughput analysis and graph-based clustering, isolating 68 satDNA families. By physically mapping these sequences in female metaphases, we discovered 15 of the most abundant satDNAs clustered in its chromosomes, 9 of which were found exclusively in the highly heterochromatic W-1. This heteromorphic sex chromosome showed the highest amount of satDNA accumulations in this species. The second most abundant family, MelSat02-26, shared FISH signals with the NOR-bearing pair in similar patterns and is linked to the multiple sex chromosome system. Our results demonstrate the diverse satDNA content in M. elongatus, especially in its heteromorphic sex chromosome. Additionally, we highlighted the different accumulation patterns and distribution of these sequences across species by physically mapping these satDNAs in other Anostomidae, Megaleporinusmacrocephalus and Leporinusfriderici (a species without differentiated sex chromosomes).Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)UNESP Univ Estadual Paulista Julio de Mesquita Fi, Dept Biol, Inst Biociencias, Rio Claro, BrazilUNESP Univ Estadual Paulista Julio de Mesquita Fi, Dept Biol, Inst Biociencias, Rio Claro, BrazilFAPESP: 2017/00195_7CAPES: 001KargerUniversidade Estadual Paulista (Unesp)Crepaldi, Carolina [UNESP]Parise-Maltempi, Patricia P. [UNESP]2020-12-10T19:56:17Z2020-12-10T19:56:17Z2020-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article38-46http://dx.doi.org/10.1159/000506265Cytogenetic And Genome Research. Basel: Karger, v. 160, n. 1, p. 38-46, 2020.1424-8581http://hdl.handle.net/11449/19679010.1159/000506265WOS:000526986400007Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCytogenetic And Genome Researchinfo:eu-repo/semantics/openAccess2021-10-23T10:02:21Zoai:repositorio.unesp.br:11449/196790Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:24:52.758770Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Heteromorphic Sex Chromosomes and Their DNA Content in Fish: An Insight through Satellite DNA Accumulation in Megaleporinus elongatus |
title |
Heteromorphic Sex Chromosomes and Their DNA Content in Fish: An Insight through Satellite DNA Accumulation in Megaleporinus elongatus |
spellingShingle |
Heteromorphic Sex Chromosomes and Their DNA Content in Fish: An Insight through Satellite DNA Accumulation in Megaleporinus elongatus Crepaldi, Carolina [UNESP] FISH Heteromorphic sex chromosome system Neotropical fish Satellitome Sex chromosome evolution |
title_short |
Heteromorphic Sex Chromosomes and Their DNA Content in Fish: An Insight through Satellite DNA Accumulation in Megaleporinus elongatus |
title_full |
Heteromorphic Sex Chromosomes and Their DNA Content in Fish: An Insight through Satellite DNA Accumulation in Megaleporinus elongatus |
title_fullStr |
Heteromorphic Sex Chromosomes and Their DNA Content in Fish: An Insight through Satellite DNA Accumulation in Megaleporinus elongatus |
title_full_unstemmed |
Heteromorphic Sex Chromosomes and Their DNA Content in Fish: An Insight through Satellite DNA Accumulation in Megaleporinus elongatus |
title_sort |
Heteromorphic Sex Chromosomes and Their DNA Content in Fish: An Insight through Satellite DNA Accumulation in Megaleporinus elongatus |
author |
Crepaldi, Carolina [UNESP] |
author_facet |
Crepaldi, Carolina [UNESP] Parise-Maltempi, Patricia P. [UNESP] |
author_role |
author |
author2 |
Parise-Maltempi, Patricia P. [UNESP] |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Crepaldi, Carolina [UNESP] Parise-Maltempi, Patricia P. [UNESP] |
dc.subject.por.fl_str_mv |
FISH Heteromorphic sex chromosome system Neotropical fish Satellitome Sex chromosome evolution |
topic |
FISH Heteromorphic sex chromosome system Neotropical fish Satellitome Sex chromosome evolution |
description |
The repetitive DNA content of fish sex chromosomes provides valuable insights into specificities and patterns of their genetic sex determination systems. In this study, we revealed the genomic satellite DNA (satDNA) content of Megaleporinuselongatus, a Neotropical fish species with Z(1)Z(1)Z(2)Z(2)/Z(1)W(1)Z(2)W(2) multiple sex chromosomes, through high-throughput analysis and graph-based clustering, isolating 68 satDNA families. By physically mapping these sequences in female metaphases, we discovered 15 of the most abundant satDNAs clustered in its chromosomes, 9 of which were found exclusively in the highly heterochromatic W-1. This heteromorphic sex chromosome showed the highest amount of satDNA accumulations in this species. The second most abundant family, MelSat02-26, shared FISH signals with the NOR-bearing pair in similar patterns and is linked to the multiple sex chromosome system. Our results demonstrate the diverse satDNA content in M. elongatus, especially in its heteromorphic sex chromosome. Additionally, we highlighted the different accumulation patterns and distribution of these sequences across species by physically mapping these satDNAs in other Anostomidae, Megaleporinusmacrocephalus and Leporinusfriderici (a species without differentiated sex chromosomes). |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-10T19:56:17Z 2020-12-10T19:56:17Z 2020-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1159/000506265 Cytogenetic And Genome Research. Basel: Karger, v. 160, n. 1, p. 38-46, 2020. 1424-8581 http://hdl.handle.net/11449/196790 10.1159/000506265 WOS:000526986400007 |
url |
http://dx.doi.org/10.1159/000506265 http://hdl.handle.net/11449/196790 |
identifier_str_mv |
Cytogenetic And Genome Research. Basel: Karger, v. 160, n. 1, p. 38-46, 2020. 1424-8581 10.1159/000506265 WOS:000526986400007 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Cytogenetic And Genome Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
38-46 |
dc.publisher.none.fl_str_mv |
Karger |
publisher.none.fl_str_mv |
Karger |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129518543568896 |