Absolute quantification of Aggregatibacter actinomycetemcomitans in patients carrying haplotypes associated with susceptibility to chronic periodontitis: Multifaceted evaluation with periodontitis covariants
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1093/femspd/ftx092 http://hdl.handle.net/11449/170443 |
Resumo: | This study aimed to evaluate the association between haplotypes in the interleukin 8 (IL8) and IL4 genes previously associated to chronic periodontitis (CP) and the levels of Aggregatibacter actinomycetemcomitans (A.a.) in subgingival sites of patients with and without CP. Moreover, multifaceted evaluations were made to search associations among patients' genetic background with the A.a. levels and previous clinical/immunological/microbiological findings. Subgingival sites (n = 596) of 104 patients were divided into susceptible to CP by the IL8 haplotype ATC/TTC (IL8+); non-susceptible to CP by the IL8 AGT/TTC (IL8-); susceptible to CP by the IL4 TCI/CCI (IL4+); protection against CP by the IL4 TTD/CTI (IL4-). Subgingival biofilm samples from diseased and healthy sites of CP patients and from control sites of health patients were obtained for absolute quantification of A.a. by quantitative real-time polymerase chain reaction. For diseased sites, samples were collected before and 45 days after periodontal treatment. The IL4 but not the IL8 haplotypes were associated with levels of A.a. (in both periods). After periodontal treatment, higher levels of A.a. were found in subgingival sites of (IL4-) patients, and higher levels of IL-4 were associated with deeper probing pockets in these same patients. Significant correlations were found among genetic (patients carrying IL8 or IL4 haplotypes), microbiological and immunological data showing the interrelationship of different factors in the CP. |
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Absolute quantification of Aggregatibacter actinomycetemcomitans in patients carrying haplotypes associated with susceptibility to chronic periodontitis: Multifaceted evaluation with periodontitis covariantsAggregatibacter actinomycetemcomitansChronic periodontitisGenetic polymorphismInterleukin-4Interleukin-8This study aimed to evaluate the association between haplotypes in the interleukin 8 (IL8) and IL4 genes previously associated to chronic periodontitis (CP) and the levels of Aggregatibacter actinomycetemcomitans (A.a.) in subgingival sites of patients with and without CP. Moreover, multifaceted evaluations were made to search associations among patients' genetic background with the A.a. levels and previous clinical/immunological/microbiological findings. Subgingival sites (n = 596) of 104 patients were divided into susceptible to CP by the IL8 haplotype ATC/TTC (IL8+); non-susceptible to CP by the IL8 AGT/TTC (IL8-); susceptible to CP by the IL4 TCI/CCI (IL4+); protection against CP by the IL4 TTD/CTI (IL4-). Subgingival biofilm samples from diseased and healthy sites of CP patients and from control sites of health patients were obtained for absolute quantification of A.a. by quantitative real-time polymerase chain reaction. For diseased sites, samples were collected before and 45 days after periodontal treatment. The IL4 but not the IL8 haplotypes were associated with levels of A.a. (in both periods). After periodontal treatment, higher levels of A.a. were found in subgingival sites of (IL4-) patients, and higher levels of IL-4 were associated with deeper probing pockets in these same patients. Significant correlations were found among genetic (patients carrying IL8 or IL4 haplotypes), microbiological and immunological data showing the interrelationship of different factors in the CP.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Morphology São Paulo State University (UNESP) School of DentistryDepartment of Oral Diagnosis and Surgery São Paulo State University (UNESP) School of DentistryDepartment of Microbiology Institute of Biomedical Sciences University of São Paulo (USP)Department of Morphology São Paulo State University (UNESP) School of DentistryDepartment of Oral Diagnosis and Surgery São Paulo State University (UNESP) School of DentistryFAPESP: 2009/08773-3Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Cirelli, Thamiris [UNESP]Finoti, Livia S. [UNESP]Corbi, Sâmia C.T. [UNESP]Anovazzi, Giovana [UNESP]Nepomuceno, Rafael [UNESP]Orrico, Silvana R.P. [UNESP]Cirelli, Joni A. [UNESP]Mayer, Márcia P.A.Scarel-Caminaga, Raquel M. [UNESP]2018-12-11T16:50:50Z2018-12-11T16:50:50Z2017-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1093/femspd/ftx092Pathogens and Disease, v. 75, n. 7, 2017.2049-632Xhttp://hdl.handle.net/11449/17044310.1093/femspd/ftx0922-s2.0-850370432392-s2.0-85037043239.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPathogens and Diseaseinfo:eu-repo/semantics/openAccess2023-12-19T06:23:58Zoai:repositorio.unesp.br:11449/170443Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-19T06:23:58Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Absolute quantification of Aggregatibacter actinomycetemcomitans in patients carrying haplotypes associated with susceptibility to chronic periodontitis: Multifaceted evaluation with periodontitis covariants |
title |
Absolute quantification of Aggregatibacter actinomycetemcomitans in patients carrying haplotypes associated with susceptibility to chronic periodontitis: Multifaceted evaluation with periodontitis covariants |
spellingShingle |
Absolute quantification of Aggregatibacter actinomycetemcomitans in patients carrying haplotypes associated with susceptibility to chronic periodontitis: Multifaceted evaluation with periodontitis covariants Cirelli, Thamiris [UNESP] Aggregatibacter actinomycetemcomitans Chronic periodontitis Genetic polymorphism Interleukin-4 Interleukin-8 |
title_short |
Absolute quantification of Aggregatibacter actinomycetemcomitans in patients carrying haplotypes associated with susceptibility to chronic periodontitis: Multifaceted evaluation with periodontitis covariants |
title_full |
Absolute quantification of Aggregatibacter actinomycetemcomitans in patients carrying haplotypes associated with susceptibility to chronic periodontitis: Multifaceted evaluation with periodontitis covariants |
title_fullStr |
Absolute quantification of Aggregatibacter actinomycetemcomitans in patients carrying haplotypes associated with susceptibility to chronic periodontitis: Multifaceted evaluation with periodontitis covariants |
title_full_unstemmed |
Absolute quantification of Aggregatibacter actinomycetemcomitans in patients carrying haplotypes associated with susceptibility to chronic periodontitis: Multifaceted evaluation with periodontitis covariants |
title_sort |
Absolute quantification of Aggregatibacter actinomycetemcomitans in patients carrying haplotypes associated with susceptibility to chronic periodontitis: Multifaceted evaluation with periodontitis covariants |
author |
Cirelli, Thamiris [UNESP] |
author_facet |
Cirelli, Thamiris [UNESP] Finoti, Livia S. [UNESP] Corbi, Sâmia C.T. [UNESP] Anovazzi, Giovana [UNESP] Nepomuceno, Rafael [UNESP] Orrico, Silvana R.P. [UNESP] Cirelli, Joni A. [UNESP] Mayer, Márcia P.A. Scarel-Caminaga, Raquel M. [UNESP] |
author_role |
author |
author2 |
Finoti, Livia S. [UNESP] Corbi, Sâmia C.T. [UNESP] Anovazzi, Giovana [UNESP] Nepomuceno, Rafael [UNESP] Orrico, Silvana R.P. [UNESP] Cirelli, Joni A. [UNESP] Mayer, Márcia P.A. Scarel-Caminaga, Raquel M. [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Cirelli, Thamiris [UNESP] Finoti, Livia S. [UNESP] Corbi, Sâmia C.T. [UNESP] Anovazzi, Giovana [UNESP] Nepomuceno, Rafael [UNESP] Orrico, Silvana R.P. [UNESP] Cirelli, Joni A. [UNESP] Mayer, Márcia P.A. Scarel-Caminaga, Raquel M. [UNESP] |
dc.subject.por.fl_str_mv |
Aggregatibacter actinomycetemcomitans Chronic periodontitis Genetic polymorphism Interleukin-4 Interleukin-8 |
topic |
Aggregatibacter actinomycetemcomitans Chronic periodontitis Genetic polymorphism Interleukin-4 Interleukin-8 |
description |
This study aimed to evaluate the association between haplotypes in the interleukin 8 (IL8) and IL4 genes previously associated to chronic periodontitis (CP) and the levels of Aggregatibacter actinomycetemcomitans (A.a.) in subgingival sites of patients with and without CP. Moreover, multifaceted evaluations were made to search associations among patients' genetic background with the A.a. levels and previous clinical/immunological/microbiological findings. Subgingival sites (n = 596) of 104 patients were divided into susceptible to CP by the IL8 haplotype ATC/TTC (IL8+); non-susceptible to CP by the IL8 AGT/TTC (IL8-); susceptible to CP by the IL4 TCI/CCI (IL4+); protection against CP by the IL4 TTD/CTI (IL4-). Subgingival biofilm samples from diseased and healthy sites of CP patients and from control sites of health patients were obtained for absolute quantification of A.a. by quantitative real-time polymerase chain reaction. For diseased sites, samples were collected before and 45 days after periodontal treatment. The IL4 but not the IL8 haplotypes were associated with levels of A.a. (in both periods). After periodontal treatment, higher levels of A.a. were found in subgingival sites of (IL4-) patients, and higher levels of IL-4 were associated with deeper probing pockets in these same patients. Significant correlations were found among genetic (patients carrying IL8 or IL4 haplotypes), microbiological and immunological data showing the interrelationship of different factors in the CP. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-10-01 2018-12-11T16:50:50Z 2018-12-11T16:50:50Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1093/femspd/ftx092 Pathogens and Disease, v. 75, n. 7, 2017. 2049-632X http://hdl.handle.net/11449/170443 10.1093/femspd/ftx092 2-s2.0-85037043239 2-s2.0-85037043239.pdf |
url |
http://dx.doi.org/10.1093/femspd/ftx092 http://hdl.handle.net/11449/170443 |
identifier_str_mv |
Pathogens and Disease, v. 75, n. 7, 2017. 2049-632X 10.1093/femspd/ftx092 2-s2.0-85037043239 2-s2.0-85037043239.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pathogens and Disease |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965338978222080 |