Oral vaccination of piglets against Mycoplasma hyopneumoniae using silica SBA-15 as an adjuvant effectively reduced consolidation lung lesions at slaughter

Detalhes bibliográficos
Autor(a) principal: Mechler-Dreibi, Marina L. [UNESP]
Data de Publicação: 2021
Outros Autores: Almeida, Henrique M. S. [UNESP], Sonalio, Karina [UNESP], Martines, Mariela A. C. [UNESP], Petri, Fernando A. M. [UNESP], Zambotti, Beatriz B. [UNESP], Ferreira, Marcela M. [UNESP], Storino, Gabriel Y. [UNESP], Martins, Tereza S., Montassier, Hélio J. [UNESP], Sant’Anna, Osvaldo A., Fantini, Márcia C. A., de Oliveira, Luís Guilherme [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1038/s41598-021-01883-2
http://hdl.handle.net/11449/222881
Resumo: Mycoplasma (M.) hyopneumoniae is the main pathogen of porcine enzootic pneumonia (PEP). Its controlling is challenging, and requires alternative strategies. This study aimed to develop an oral vaccine against M. hyopneumoniae using a nanostructured mesoporous silica (SBA-15) as an adjuvant, and compare its effect with an intramuscular (IM) commercial vaccine (CV). Fifty 24 day-old M. hyopneumoniae-free piglets composed five equal groups for different immunization protocols, consisting of a CV and/or oral immunization (OI). Control piglets did not receive any form of immunization. All piglets were challenged with M. hyopneumoniae strain 232 on D49 by tracheal route. IgA antibody response in the respiratory tract, bacterial shedding and serum IgG were evaluated. The piglets were euthanized on 28 (D77) and 56 (D105) days post-infection. Lung lesions were macroscopically evaluated; lung fragments and bronchoalveolar fluid (BALF) were collected for estimation of bacterial loads by qPCR and/or histopathology examination. All immunization protocols induced reduction on Mycoplasma-like macroscopic lung lesions. IgA Ab responses anti-M. hyopneumoniae, the expression of IL-4 cytokine and a lower expression of IL-8 were induced by CV and OI vaccines, while IgG was induced only by CV. Oral immunization using silica as a carrier-adjuvant can be viable in controlling M. hyopneumoniae infection.
id UNSP_93b5b89764b862197c1eea76e0508ada
oai_identifier_str oai:repositorio.unesp.br:11449/222881
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Oral vaccination of piglets against Mycoplasma hyopneumoniae using silica SBA-15 as an adjuvant effectively reduced consolidation lung lesions at slaughterMycoplasma (M.) hyopneumoniae is the main pathogen of porcine enzootic pneumonia (PEP). Its controlling is challenging, and requires alternative strategies. This study aimed to develop an oral vaccine against M. hyopneumoniae using a nanostructured mesoporous silica (SBA-15) as an adjuvant, and compare its effect with an intramuscular (IM) commercial vaccine (CV). Fifty 24 day-old M. hyopneumoniae-free piglets composed five equal groups for different immunization protocols, consisting of a CV and/or oral immunization (OI). Control piglets did not receive any form of immunization. All piglets were challenged with M. hyopneumoniae strain 232 on D49 by tracheal route. IgA antibody response in the respiratory tract, bacterial shedding and serum IgG were evaluated. The piglets were euthanized on 28 (D77) and 56 (D105) days post-infection. Lung lesions were macroscopically evaluated; lung fragments and bronchoalveolar fluid (BALF) were collected for estimation of bacterial loads by qPCR and/or histopathology examination. All immunization protocols induced reduction on Mycoplasma-like macroscopic lung lesions. IgA Ab responses anti-M. hyopneumoniae, the expression of IL-4 cytokine and a lower expression of IL-8 were induced by CV and OI vaccines, while IgG was induced only by CV. Oral immunization using silica as a carrier-adjuvant can be viable in controlling M. hyopneumoniae infection.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)School of Agricultural and Veterinarian Sciences São Paulo State University (Unesp)Department of Chemistry Federal University of São Paulo (UNIFESP)Butantan InstitutePhysics Institute University of São Paulo (USP)School of Agricultural and Veterinarian Sciences São Paulo State University (Unesp)FAPESP: #2018/12742-5FAPESP: #2019/19710-4Universidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Butantan InstituteMechler-Dreibi, Marina L. [UNESP]Almeida, Henrique M. S. [UNESP]Sonalio, Karina [UNESP]Martines, Mariela A. C. [UNESP]Petri, Fernando A. M. [UNESP]Zambotti, Beatriz B. [UNESP]Ferreira, Marcela M. [UNESP]Storino, Gabriel Y. [UNESP]Martins, Tereza S.Montassier, Hélio J. [UNESP]Sant’Anna, Osvaldo A.Fantini, Márcia C. A.de Oliveira, Luís Guilherme [UNESP]2022-04-28T19:47:15Z2022-04-28T19:47:15Z2021-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1038/s41598-021-01883-2Scientific Reports, v. 11, n. 1, 2021.2045-2322http://hdl.handle.net/11449/22288110.1038/s41598-021-01883-22-s2.0-85119282981Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientific Reportsinfo:eu-repo/semantics/openAccess2022-04-28T19:47:16Zoai:repositorio.unesp.br:11449/222881Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:36:04.410048Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Oral vaccination of piglets against Mycoplasma hyopneumoniae using silica SBA-15 as an adjuvant effectively reduced consolidation lung lesions at slaughter
title Oral vaccination of piglets against Mycoplasma hyopneumoniae using silica SBA-15 as an adjuvant effectively reduced consolidation lung lesions at slaughter
spellingShingle Oral vaccination of piglets against Mycoplasma hyopneumoniae using silica SBA-15 as an adjuvant effectively reduced consolidation lung lesions at slaughter
Mechler-Dreibi, Marina L. [UNESP]
title_short Oral vaccination of piglets against Mycoplasma hyopneumoniae using silica SBA-15 as an adjuvant effectively reduced consolidation lung lesions at slaughter
title_full Oral vaccination of piglets against Mycoplasma hyopneumoniae using silica SBA-15 as an adjuvant effectively reduced consolidation lung lesions at slaughter
title_fullStr Oral vaccination of piglets against Mycoplasma hyopneumoniae using silica SBA-15 as an adjuvant effectively reduced consolidation lung lesions at slaughter
title_full_unstemmed Oral vaccination of piglets against Mycoplasma hyopneumoniae using silica SBA-15 as an adjuvant effectively reduced consolidation lung lesions at slaughter
title_sort Oral vaccination of piglets against Mycoplasma hyopneumoniae using silica SBA-15 as an adjuvant effectively reduced consolidation lung lesions at slaughter
author Mechler-Dreibi, Marina L. [UNESP]
author_facet Mechler-Dreibi, Marina L. [UNESP]
Almeida, Henrique M. S. [UNESP]
Sonalio, Karina [UNESP]
Martines, Mariela A. C. [UNESP]
Petri, Fernando A. M. [UNESP]
Zambotti, Beatriz B. [UNESP]
Ferreira, Marcela M. [UNESP]
Storino, Gabriel Y. [UNESP]
Martins, Tereza S.
Montassier, Hélio J. [UNESP]
Sant’Anna, Osvaldo A.
Fantini, Márcia C. A.
de Oliveira, Luís Guilherme [UNESP]
author_role author
author2 Almeida, Henrique M. S. [UNESP]
Sonalio, Karina [UNESP]
Martines, Mariela A. C. [UNESP]
Petri, Fernando A. M. [UNESP]
Zambotti, Beatriz B. [UNESP]
Ferreira, Marcela M. [UNESP]
Storino, Gabriel Y. [UNESP]
Martins, Tereza S.
Montassier, Hélio J. [UNESP]
Sant’Anna, Osvaldo A.
Fantini, Márcia C. A.
de Oliveira, Luís Guilherme [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Universidade de São Paulo (USP)
Butantan Institute
dc.contributor.author.fl_str_mv Mechler-Dreibi, Marina L. [UNESP]
Almeida, Henrique M. S. [UNESP]
Sonalio, Karina [UNESP]
Martines, Mariela A. C. [UNESP]
Petri, Fernando A. M. [UNESP]
Zambotti, Beatriz B. [UNESP]
Ferreira, Marcela M. [UNESP]
Storino, Gabriel Y. [UNESP]
Martins, Tereza S.
Montassier, Hélio J. [UNESP]
Sant’Anna, Osvaldo A.
Fantini, Márcia C. A.
de Oliveira, Luís Guilherme [UNESP]
description Mycoplasma (M.) hyopneumoniae is the main pathogen of porcine enzootic pneumonia (PEP). Its controlling is challenging, and requires alternative strategies. This study aimed to develop an oral vaccine against M. hyopneumoniae using a nanostructured mesoporous silica (SBA-15) as an adjuvant, and compare its effect with an intramuscular (IM) commercial vaccine (CV). Fifty 24 day-old M. hyopneumoniae-free piglets composed five equal groups for different immunization protocols, consisting of a CV and/or oral immunization (OI). Control piglets did not receive any form of immunization. All piglets were challenged with M. hyopneumoniae strain 232 on D49 by tracheal route. IgA antibody response in the respiratory tract, bacterial shedding and serum IgG were evaluated. The piglets were euthanized on 28 (D77) and 56 (D105) days post-infection. Lung lesions were macroscopically evaluated; lung fragments and bronchoalveolar fluid (BALF) were collected for estimation of bacterial loads by qPCR and/or histopathology examination. All immunization protocols induced reduction on Mycoplasma-like macroscopic lung lesions. IgA Ab responses anti-M. hyopneumoniae, the expression of IL-4 cytokine and a lower expression of IL-8 were induced by CV and OI vaccines, while IgG was induced only by CV. Oral immunization using silica as a carrier-adjuvant can be viable in controlling M. hyopneumoniae infection.
publishDate 2021
dc.date.none.fl_str_mv 2021-12-01
2022-04-28T19:47:15Z
2022-04-28T19:47:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1038/s41598-021-01883-2
Scientific Reports, v. 11, n. 1, 2021.
2045-2322
http://hdl.handle.net/11449/222881
10.1038/s41598-021-01883-2
2-s2.0-85119282981
url http://dx.doi.org/10.1038/s41598-021-01883-2
http://hdl.handle.net/11449/222881
identifier_str_mv Scientific Reports, v. 11, n. 1, 2021.
2045-2322
10.1038/s41598-021-01883-2
2-s2.0-85119282981
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Scientific Reports
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129535338610688