Aspectos evolutivos e transcricionais da família gênica da catalase em genomas vegetais

• Autor(a) principal: Calzado, Natália Fermino [UNESP]
• Data de Publicação: 2016
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/156030; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2017-09-28/000890019.pdf
• topic: Genetica vegetal; Evolução molecular; Antioxidantes; Genoma; Catalase

The photosynthesis and aerobic respiration are important energy getting mechanisms in living beings. Both processes pass through cell oxidation, which can be enhanced under stress, causing an overproduction of reactive oxygen species (ROS), of which an example is hydrogen peroxide (H2O2). Cells have multiple systems to prevent damage caused by ROS, among which the antioxidant enzyme system. One of the enzymes involved in this system is catalase. This enzyme acts on H2O2 degradation and is encoded by a gene family that in Arabidopsis thaliana is composed of three genes. The aim of this study was to annotate and analysis phylogenetic the catalase gene family in 14 plant species, as well as transcriptional regulations for species selected based on literature data. Only four gene family owned species had 3 genescodificantes to catalase. Evolutionary analyses indicated that the catalase in dicots are more conserved than in monocots, there are missing phylogenetic groups of catalase in model species A. thaliana genes of this gene family go through purifying selection, but with substitution pattern dissimilar between codons. Inferences Transcriptional in selected species indicate that each member of the gene family has a tissue specific transcriptional profile they are differentially regulated by stress. Thus, this work brings elements that emphasize the importance of evolutionary analyses together with biochemical analysis for understanding cellular metabolism molecular responses