Estresse salino em plantas jovens de cana-deaçúcar: respostas bioquímicas e fisiológicas
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/133987 |
Resumo: | Sugarcane is a globally important crop. Brazil is the largest producer of sugarcane and stands out in the marketing and exportation of sugar and ethanol derived from this culture. The various abiotic stresses adversely change plant growth and between the stresses, soil salinity is presented by the severe reduction in productivity, it is the main stressor in arid and semi-arid climates. This study aimed to verify the effects of NaCl on the development of sugarcane young plants, SP 81- 3250 and IAC 87-3396 cultivars, through the study of physiological-biochemical variables: (I) the osmoprotectors proline, glycine betaine and sucrose, (II) the occurrence of nutritional changes the ratio Na+/K+ (III) lipid peroxidation (IV) effect of NaCl in the activity of nitrate reductase enzyme; (V) the effect of salt stress on gas exchange; (VI) the growth of shoots and roots of the sugarcane cultivars under salt stress. Thus, it was used four concentrations of NaCl (0, 40, 80 and 160 mM) and made the following assessments: Na+ and K+ accumulation in the leaves and roots, compatible osmolytes: proline, glycine betaine and sucrose, water potential, peroxidation lipid, activity of nitrate reductase enzyme, gas exchange, total chlorophyll, quantum yield of PSII and shoot and root growth at 15 and 30 days of development after stress application. The results showed higher susceptibility of the cv. IAC 87-3396 by higher accumulation of Na+ and Na+/K+ ratio, increase in lipid peroxidation by MDA accumulation and greater negative effect on gas exchange. Both cultivars showed proline accumulation under stress, however, the cv. SP 81- 3250 showed in addition faster accumulation of this amino acid, reduced lipid peroxidation and higher growth. Under severe salt stress cv. IAC 87-3396 is more susceptible. |
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Estresse salino em plantas jovens de cana-deaçúcar: respostas bioquímicas e fisiológicasSalt stress in young sugarcane plants: biochemical and physiological responsesPhotosynthesisOsmoprotectorsNitrate reductaseFotossínteseNaClOsmoprotetoresPeroxidação lipídicaRedutase do nitratoSaccharum sppLipid peroxidatioSugarcane is a globally important crop. Brazil is the largest producer of sugarcane and stands out in the marketing and exportation of sugar and ethanol derived from this culture. The various abiotic stresses adversely change plant growth and between the stresses, soil salinity is presented by the severe reduction in productivity, it is the main stressor in arid and semi-arid climates. This study aimed to verify the effects of NaCl on the development of sugarcane young plants, SP 81- 3250 and IAC 87-3396 cultivars, through the study of physiological-biochemical variables: (I) the osmoprotectors proline, glycine betaine and sucrose, (II) the occurrence of nutritional changes the ratio Na+/K+ (III) lipid peroxidation (IV) effect of NaCl in the activity of nitrate reductase enzyme; (V) the effect of salt stress on gas exchange; (VI) the growth of shoots and roots of the sugarcane cultivars under salt stress. Thus, it was used four concentrations of NaCl (0, 40, 80 and 160 mM) and made the following assessments: Na+ and K+ accumulation in the leaves and roots, compatible osmolytes: proline, glycine betaine and sucrose, water potential, peroxidation lipid, activity of nitrate reductase enzyme, gas exchange, total chlorophyll, quantum yield of PSII and shoot and root growth at 15 and 30 days of development after stress application. The results showed higher susceptibility of the cv. IAC 87-3396 by higher accumulation of Na+ and Na+/K+ ratio, increase in lipid peroxidation by MDA accumulation and greater negative effect on gas exchange. Both cultivars showed proline accumulation under stress, however, the cv. SP 81- 3250 showed in addition faster accumulation of this amino acid, reduced lipid peroxidation and higher growth. Under severe salt stress cv. IAC 87-3396 is more susceptible.A cana-de-açúcar é uma cultura mundialmente importante. O Brasil é o maior produtor mundial de cana-de-açúcar e se destaca na comercialização e exportação de açúcar e etanol originados a partir desta cultura. Os diferentes estresses abióticos alteram negativamente o desenvolvimento das plantas e, entre os estresses, a salinidade de solos se apresenta pela grave redução de produtividade, sendo o principal fator estressante em regiões de clima árido e semiárido. Este trabalho objetivou verificar os efeitos do NaCl sobre o desenvolvimento de plantas jovens de cana-de-açúcar, cultivares SP 81-3250 e IAC 87-3396, mediante o estudo de variáveis fisiológicas-bioquímicas: (I) os osmoprotetores prolina, glicina betaína e sacarose, (II) a ocorrência de alterações nutricionais pela relação Na+/K+, (III) a peroxidação lipídica (IV) a influência do NaCl na atividade da enzima redutase do nitrato; (V) o efeito do estresse salino nas trocas gasosas; (VI) o crescimento de parte aérea e raízes das diferentes cultivares de cana-de-açúcar sob estresse salino. Para tanto, foram utilizadas quatro concentrações de NaCl (0, 40, 80 e 160 mM) e realizadas as seguintes avaliações: teores de Na+ e K+ nas folhas e raízes, os osmólitos compatíveis prolina, glicina betaína e sacarose, potencial hídrico, a peroxidação lipídica, atividade da enzima redutase do nitrato, trocas gasosas, clorofilas totais, rendimento quântico do FSII e crescimento de parte aérea e raiz aos 15 e 30 dias de desenvolvimento após aplicação do estresse. A partir dos resultados, observou-se maior suscetibilidade das plantas da cv. IAC 87-3396 pelo maior acúmulo de Na+ e relação Na+/K+, maior peroxidação lipídica pelo acúmulo de MDA e maior efeito negativo sobre as trocas gasosas. Ambas as cultivares apresentaram acúmulo de prolina sob estresse, porém, plantas da cv. SP 81-3250 apresentaram, além de mais rápido acúmulo deste aminoácido, menor peroxidação lipídica e crescimento superior. Sob estresse salino severo a cv. IAC 87-3396 apresenta maior suscetibilidade.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Estadual Paulista (Unesp)Santos, Durvalina Maria Mathias dos [UNESP]Munns, RanaUniversidade Estadual Paulista (Unesp)Chiconato, Denise Aparecida [UNESP]2016-02-05T12:07:51Z2016-02-05T12:07:51Z2016-01-22info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfhttp://hdl.handle.net/11449/13398700086576233004102001P488595641821396360000-0002-2158-280Xporinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESP2024-06-05T15:17:42Zoai:repositorio.unesp.br:11449/133987Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:32:26.517926Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Estresse salino em plantas jovens de cana-deaçúcar: respostas bioquímicas e fisiológicas Salt stress in young sugarcane plants: biochemical and physiological responses |
title |
Estresse salino em plantas jovens de cana-deaçúcar: respostas bioquímicas e fisiológicas |
spellingShingle |
Estresse salino em plantas jovens de cana-deaçúcar: respostas bioquímicas e fisiológicas Chiconato, Denise Aparecida [UNESP] Photosynthesis Osmoprotectors Nitrate reductase Fotossíntese NaCl Osmoprotetores Peroxidação lipídica Redutase do nitrato Saccharum spp Lipid peroxidatio |
title_short |
Estresse salino em plantas jovens de cana-deaçúcar: respostas bioquímicas e fisiológicas |
title_full |
Estresse salino em plantas jovens de cana-deaçúcar: respostas bioquímicas e fisiológicas |
title_fullStr |
Estresse salino em plantas jovens de cana-deaçúcar: respostas bioquímicas e fisiológicas |
title_full_unstemmed |
Estresse salino em plantas jovens de cana-deaçúcar: respostas bioquímicas e fisiológicas |
title_sort |
Estresse salino em plantas jovens de cana-deaçúcar: respostas bioquímicas e fisiológicas |
author |
Chiconato, Denise Aparecida [UNESP] |
author_facet |
Chiconato, Denise Aparecida [UNESP] |
author_role |
author |
dc.contributor.none.fl_str_mv |
Santos, Durvalina Maria Mathias dos [UNESP] Munns, Rana Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Chiconato, Denise Aparecida [UNESP] |
dc.subject.por.fl_str_mv |
Photosynthesis Osmoprotectors Nitrate reductase Fotossíntese NaCl Osmoprotetores Peroxidação lipídica Redutase do nitrato Saccharum spp Lipid peroxidatio |
topic |
Photosynthesis Osmoprotectors Nitrate reductase Fotossíntese NaCl Osmoprotetores Peroxidação lipídica Redutase do nitrato Saccharum spp Lipid peroxidatio |
description |
Sugarcane is a globally important crop. Brazil is the largest producer of sugarcane and stands out in the marketing and exportation of sugar and ethanol derived from this culture. The various abiotic stresses adversely change plant growth and between the stresses, soil salinity is presented by the severe reduction in productivity, it is the main stressor in arid and semi-arid climates. This study aimed to verify the effects of NaCl on the development of sugarcane young plants, SP 81- 3250 and IAC 87-3396 cultivars, through the study of physiological-biochemical variables: (I) the osmoprotectors proline, glycine betaine and sucrose, (II) the occurrence of nutritional changes the ratio Na+/K+ (III) lipid peroxidation (IV) effect of NaCl in the activity of nitrate reductase enzyme; (V) the effect of salt stress on gas exchange; (VI) the growth of shoots and roots of the sugarcane cultivars under salt stress. Thus, it was used four concentrations of NaCl (0, 40, 80 and 160 mM) and made the following assessments: Na+ and K+ accumulation in the leaves and roots, compatible osmolytes: proline, glycine betaine and sucrose, water potential, peroxidation lipid, activity of nitrate reductase enzyme, gas exchange, total chlorophyll, quantum yield of PSII and shoot and root growth at 15 and 30 days of development after stress application. The results showed higher susceptibility of the cv. IAC 87-3396 by higher accumulation of Na+ and Na+/K+ ratio, increase in lipid peroxidation by MDA accumulation and greater negative effect on gas exchange. Both cultivars showed proline accumulation under stress, however, the cv. SP 81- 3250 showed in addition faster accumulation of this amino acid, reduced lipid peroxidation and higher growth. Under severe salt stress cv. IAC 87-3396 is more susceptible. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-02-05T12:07:51Z 2016-02-05T12:07:51Z 2016-01-22 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/11449/133987 000865762 33004102001P4 8859564182139636 0000-0002-2158-280X |
url |
http://hdl.handle.net/11449/133987 |
identifier_str_mv |
000865762 33004102001P4 8859564182139636 0000-0002-2158-280X |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
publisher.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
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UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129435295023104 |