Ehrlichia canis (Jaboticabal strain) induces the expression of TNF-alpha in leukocytes and splenocytes of experimentally infected dogs
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1984-29612011000100015 http://hdl.handle.net/11449/130611 |
Resumo: | Canine ehrlichiosis is caused by the bacterium Ehrlichia canis and is characterized by a systemic febrile disease of unknown pathogenesis. This study evaluated the expression of cytokines TNF-alpha, IL-10, IFN-gamma, in splenic cells and blood leukocytes during the acute phase of ehrlichiosis and after treatment with doxycycline hyclate in dogs experimentally infected with the E. canis Jaboticabal strain. The study results showed a significant expression of TNF-alpha 18 days post-inoculation, reducing by approximately 70% after treatment. There was a unique peak of expression of IL-10 and IFN-gamma 18 and 30 days post-inoculation, respectively. This study suggests that TNF-alpha plays a role in the pathogenesis of the acute phase of canine ehrlichiosis and that treatment with doxycycline hyclate reduces the systemic effects of this cytokine, possibly by reducing or eliminating parasitemia. |
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Repositório Institucional da UNESP |
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Ehrlichia canis (Jaboticabal strain) induces the expression of TNF-alpha in leukocytes and splenocytes of experimentally infected dogsAmostra Ehrlichia canis (Jaboticabal) induz a expressão de TNF-alfa em leucócitos e esplenócitos de cães experimentalmente infectadosEhrlichiosisDogExperimental infectionIL-10IFN-γErliquioseCãoInfecção experimentalIL-10IFN-γCanine ehrlichiosis is caused by the bacterium Ehrlichia canis and is characterized by a systemic febrile disease of unknown pathogenesis. This study evaluated the expression of cytokines TNF-alpha, IL-10, IFN-gamma, in splenic cells and blood leukocytes during the acute phase of ehrlichiosis and after treatment with doxycycline hyclate in dogs experimentally infected with the E. canis Jaboticabal strain. The study results showed a significant expression of TNF-alpha 18 days post-inoculation, reducing by approximately 70% after treatment. There was a unique peak of expression of IL-10 and IFN-gamma 18 and 30 days post-inoculation, respectively. This study suggests that TNF-alpha plays a role in the pathogenesis of the acute phase of canine ehrlichiosis and that treatment with doxycycline hyclate reduces the systemic effects of this cytokine, possibly by reducing or eliminating parasitemia.A erliquiose canina é causada pela bactéria Ehrlichia canis, que desencadeia no hospedeiro uma doença febril e sistêmica, de patogênese pouco conhecida. O presente estudo avaliou a expressão das citocinas TNF-α, IL-10, IFN-γ, em células esplênicas e em leucócitos sanguíneos, durante a fase aguda da erliquiose e após o tratamento com hiclato de doxiciclina, em cães experimentalmente infectados com a amostra E. canis Jaboticabal. Os resultados mostraram expressão significativa de TNF-α 18 dias após a inoculação, reduzindo aproximadante 70% após o tratamento. Houve um único pico de expressão de IL-10 e de IFN-γ entre 18 e 30 dias após a inoculação, respectivamente. Este estudo sugere que o TNF-α participa da patogenia da fase aguda da erliquiose canina, e que o tratamento com hiclato de doxiciclina reduz os efeitos sistêmicos dessa citocina, possivelmente por reduzir ou eliminar a parasitemia.Universidade de UberabaUniversidade Estadual PaulistaUniversidade Federal do Pará (UFPA)Universidade Estadual PaulistaColégio Brasileiro de Parasitologia VeterináriaUniversidade de Uberaba (UNIUBE)Universidade Estadual Paulista (Unesp)Universidade Federal do Pará (UFPA)Faria, Joice Lara MaiaMunhoz, Thiago Demarchi [UNESP]João, Carolina FranchiVargas-Hernández, Giovanny [UNESP]André, Marcos Rogério [UNESP]Pereira, Wanderson Adriano BiscolaMachado, Rosângela Zacarias [UNESP]Tinucci-Costa, Mirela [UNESP]2014-05-20T13:15:57Z2014-05-20T13:15:57Z2011-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article71-74application/pdfhttp://dx.doi.org/10.1590/S1984-29612011000100015Revista Brasileira de Parasitologia Veterinaria. São Paulo: Brazilian Coll Veterinary Parasitology, v. 20, n. 1, p. 71-74, 2011.0103-846X1984-2961http://hdl.handle.net/11449/13061110.1590/S1984-29612011000100015S1984-29612011000100015WOS:0003028421000152-s2.0-79955162713S1984-29612011000100015.pdf32549906124518369139899895580513SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira de Parasitologia Veterinária1.090info:eu-repo/semantics/openAccess2023-12-29T06:13:55Zoai:repositorio.unesp.br:11449/130611Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-29T06:13:55Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Ehrlichia canis (Jaboticabal strain) induces the expression of TNF-alpha in leukocytes and splenocytes of experimentally infected dogs Amostra Ehrlichia canis (Jaboticabal) induz a expressão de TNF-alfa em leucócitos e esplenócitos de cães experimentalmente infectados |
title |
Ehrlichia canis (Jaboticabal strain) induces the expression of TNF-alpha in leukocytes and splenocytes of experimentally infected dogs |
spellingShingle |
Ehrlichia canis (Jaboticabal strain) induces the expression of TNF-alpha in leukocytes and splenocytes of experimentally infected dogs Faria, Joice Lara Maia Ehrlichiosis Dog Experimental infection IL-10 IFN-γ Erliquiose Cão Infecção experimental IL-10 IFN-γ |
title_short |
Ehrlichia canis (Jaboticabal strain) induces the expression of TNF-alpha in leukocytes and splenocytes of experimentally infected dogs |
title_full |
Ehrlichia canis (Jaboticabal strain) induces the expression of TNF-alpha in leukocytes and splenocytes of experimentally infected dogs |
title_fullStr |
Ehrlichia canis (Jaboticabal strain) induces the expression of TNF-alpha in leukocytes and splenocytes of experimentally infected dogs |
title_full_unstemmed |
Ehrlichia canis (Jaboticabal strain) induces the expression of TNF-alpha in leukocytes and splenocytes of experimentally infected dogs |
title_sort |
Ehrlichia canis (Jaboticabal strain) induces the expression of TNF-alpha in leukocytes and splenocytes of experimentally infected dogs |
author |
Faria, Joice Lara Maia |
author_facet |
Faria, Joice Lara Maia Munhoz, Thiago Demarchi [UNESP] João, Carolina Franchi Vargas-Hernández, Giovanny [UNESP] André, Marcos Rogério [UNESP] Pereira, Wanderson Adriano Biscola Machado, Rosângela Zacarias [UNESP] Tinucci-Costa, Mirela [UNESP] |
author_role |
author |
author2 |
Munhoz, Thiago Demarchi [UNESP] João, Carolina Franchi Vargas-Hernández, Giovanny [UNESP] André, Marcos Rogério [UNESP] Pereira, Wanderson Adriano Biscola Machado, Rosângela Zacarias [UNESP] Tinucci-Costa, Mirela [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de Uberaba (UNIUBE) Universidade Estadual Paulista (Unesp) Universidade Federal do Pará (UFPA) |
dc.contributor.author.fl_str_mv |
Faria, Joice Lara Maia Munhoz, Thiago Demarchi [UNESP] João, Carolina Franchi Vargas-Hernández, Giovanny [UNESP] André, Marcos Rogério [UNESP] Pereira, Wanderson Adriano Biscola Machado, Rosângela Zacarias [UNESP] Tinucci-Costa, Mirela [UNESP] |
dc.subject.por.fl_str_mv |
Ehrlichiosis Dog Experimental infection IL-10 IFN-γ Erliquiose Cão Infecção experimental IL-10 IFN-γ |
topic |
Ehrlichiosis Dog Experimental infection IL-10 IFN-γ Erliquiose Cão Infecção experimental IL-10 IFN-γ |
description |
Canine ehrlichiosis is caused by the bacterium Ehrlichia canis and is characterized by a systemic febrile disease of unknown pathogenesis. This study evaluated the expression of cytokines TNF-alpha, IL-10, IFN-gamma, in splenic cells and blood leukocytes during the acute phase of ehrlichiosis and after treatment with doxycycline hyclate in dogs experimentally infected with the E. canis Jaboticabal strain. The study results showed a significant expression of TNF-alpha 18 days post-inoculation, reducing by approximately 70% after treatment. There was a unique peak of expression of IL-10 and IFN-gamma 18 and 30 days post-inoculation, respectively. This study suggests that TNF-alpha plays a role in the pathogenesis of the acute phase of canine ehrlichiosis and that treatment with doxycycline hyclate reduces the systemic effects of this cytokine, possibly by reducing or eliminating parasitemia. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-03-01 2014-05-20T13:15:57Z 2014-05-20T13:15:57Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1984-29612011000100015 Revista Brasileira de Parasitologia Veterinaria. São Paulo: Brazilian Coll Veterinary Parasitology, v. 20, n. 1, p. 71-74, 2011. 0103-846X 1984-2961 http://hdl.handle.net/11449/130611 10.1590/S1984-29612011000100015 S1984-29612011000100015 WOS:000302842100015 2-s2.0-79955162713 S1984-29612011000100015.pdf 3254990612451836 9139899895580513 |
url |
http://dx.doi.org/10.1590/S1984-29612011000100015 http://hdl.handle.net/11449/130611 |
identifier_str_mv |
Revista Brasileira de Parasitologia Veterinaria. São Paulo: Brazilian Coll Veterinary Parasitology, v. 20, n. 1, p. 71-74, 2011. 0103-846X 1984-2961 10.1590/S1984-29612011000100015 S1984-29612011000100015 WOS:000302842100015 2-s2.0-79955162713 S1984-29612011000100015.pdf 3254990612451836 9139899895580513 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista Brasileira de Parasitologia Veterinária 1.090 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
71-74 application/pdf |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
publisher.none.fl_str_mv |
Colégio Brasileiro de Parasitologia Veterinária |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965440626130944 |