Lyophilized açaí pulp (Euterpe oleracea Mart) attenuates colitis-associated colon carcinogenesis while its main anthocyanin has the potential to affect the motility of colon cancer cells
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.fct.2018.08.078 http://hdl.handle.net/11449/176819 |
Resumo: | This study evaluated the possible protective effects of lyophilized açaí pulp (AP) in a colitis-associated carcinogenesis (CAC) rat model and the modifying effect of cyanidin 3-rutinoside (C3R) on the motility of RKO colon adenocarcinoma cells, using the wound healing assay. Male Wistar rats were induced to develop CAC using 1,2-dimethylhydrazine (DMH) and 2,4,6-trinitrobenzene acid (TNBS). Animals were randomly assigned to different groups that received basal diet or basal diet supplemented with 5.0% or 7.5% lyophilized AP. The findings indicate: 1) C3R (25 μM) has the potential to reduce RKO cell motility in vitro; 2) ingestion of lyophilized AP reduces the total number of aberrant crypt foci (ACF), ACF multiplicity, tumor cell proliferation and incidence of tumors with high grade dysplasia; 3) AP increases the gene expression of negative regulators of cell proliferation such as Dlc1 and Akt3, as well as inflammation (Ppara). Thus, lyophilized AP could exert a potential antitumor activity. |
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Lyophilized açaí pulp (Euterpe oleracea Mart) attenuates colitis-associated colon carcinogenesis while its main anthocyanin has the potential to affect the motility of colon cancer cellsColitisColon carcinogenesisCyanidin 3-rutinosideLyophilized açaí pulpPreventionThis study evaluated the possible protective effects of lyophilized açaí pulp (AP) in a colitis-associated carcinogenesis (CAC) rat model and the modifying effect of cyanidin 3-rutinoside (C3R) on the motility of RKO colon adenocarcinoma cells, using the wound healing assay. Male Wistar rats were induced to develop CAC using 1,2-dimethylhydrazine (DMH) and 2,4,6-trinitrobenzene acid (TNBS). Animals were randomly assigned to different groups that received basal diet or basal diet supplemented with 5.0% or 7.5% lyophilized AP. The findings indicate: 1) C3R (25 μM) has the potential to reduce RKO cell motility in vitro; 2) ingestion of lyophilized AP reduces the total number of aberrant crypt foci (ACF), ACF multiplicity, tumor cell proliferation and incidence of tumors with high grade dysplasia; 3) AP increases the gene expression of negative regulators of cell proliferation such as Dlc1 and Akt3, as well as inflammation (Ppara). Thus, lyophilized AP could exert a potential antitumor activity.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Department of Pathology Botucatu Medical School UNESPCancer Prevention and Control Program Fox Chase Cancer CenterCancer Biology Program Fox Chase Cancer CenterDepartment of Morphology Institute of Biosciences of Botucatu UNESPDepartment of Pathology Botucatu Medical School UNESPDepartment of Morphology Institute of Biosciences of Botucatu UNESPFAPESP: 2013/17600-0CAPES: PDSE 008192/2014-06Universidade Estadual Paulista (Unesp)Fox Chase Cancer CenterFragoso, Mariana F. [UNESP]Romualdo, Guilherme R. [UNESP]Vanderveer, Lisa A.Franco-Barraza, JanuszCukierman, EdnaClapper, Margie L.Carvalho, Robson F. [UNESP]Barbisan, Luis F. [UNESP]2018-12-11T17:22:37Z2018-12-11T17:22:37Z2018-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article237-245application/pdfhttp://dx.doi.org/10.1016/j.fct.2018.08.078Food and Chemical Toxicology, v. 121, p. 237-245.1873-63510278-6915http://hdl.handle.net/11449/17681910.1016/j.fct.2018.08.0782-s2.0-850530260752-s2.0-85053026075.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFood and Chemical Toxicology1,144info:eu-repo/semantics/openAccess2024-09-03T13:18:43Zoai:repositorio.unesp.br:11449/176819Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:18:43Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Lyophilized açaí pulp (Euterpe oleracea Mart) attenuates colitis-associated colon carcinogenesis while its main anthocyanin has the potential to affect the motility of colon cancer cells |
title |
Lyophilized açaí pulp (Euterpe oleracea Mart) attenuates colitis-associated colon carcinogenesis while its main anthocyanin has the potential to affect the motility of colon cancer cells |
spellingShingle |
Lyophilized açaí pulp (Euterpe oleracea Mart) attenuates colitis-associated colon carcinogenesis while its main anthocyanin has the potential to affect the motility of colon cancer cells Fragoso, Mariana F. [UNESP] Colitis Colon carcinogenesis Cyanidin 3-rutinoside Lyophilized açaí pulp Prevention |
title_short |
Lyophilized açaí pulp (Euterpe oleracea Mart) attenuates colitis-associated colon carcinogenesis while its main anthocyanin has the potential to affect the motility of colon cancer cells |
title_full |
Lyophilized açaí pulp (Euterpe oleracea Mart) attenuates colitis-associated colon carcinogenesis while its main anthocyanin has the potential to affect the motility of colon cancer cells |
title_fullStr |
Lyophilized açaí pulp (Euterpe oleracea Mart) attenuates colitis-associated colon carcinogenesis while its main anthocyanin has the potential to affect the motility of colon cancer cells |
title_full_unstemmed |
Lyophilized açaí pulp (Euterpe oleracea Mart) attenuates colitis-associated colon carcinogenesis while its main anthocyanin has the potential to affect the motility of colon cancer cells |
title_sort |
Lyophilized açaí pulp (Euterpe oleracea Mart) attenuates colitis-associated colon carcinogenesis while its main anthocyanin has the potential to affect the motility of colon cancer cells |
author |
Fragoso, Mariana F. [UNESP] |
author_facet |
Fragoso, Mariana F. [UNESP] Romualdo, Guilherme R. [UNESP] Vanderveer, Lisa A. Franco-Barraza, Janusz Cukierman, Edna Clapper, Margie L. Carvalho, Robson F. [UNESP] Barbisan, Luis F. [UNESP] |
author_role |
author |
author2 |
Romualdo, Guilherme R. [UNESP] Vanderveer, Lisa A. Franco-Barraza, Janusz Cukierman, Edna Clapper, Margie L. Carvalho, Robson F. [UNESP] Barbisan, Luis F. [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Fox Chase Cancer Center |
dc.contributor.author.fl_str_mv |
Fragoso, Mariana F. [UNESP] Romualdo, Guilherme R. [UNESP] Vanderveer, Lisa A. Franco-Barraza, Janusz Cukierman, Edna Clapper, Margie L. Carvalho, Robson F. [UNESP] Barbisan, Luis F. [UNESP] |
dc.subject.por.fl_str_mv |
Colitis Colon carcinogenesis Cyanidin 3-rutinoside Lyophilized açaí pulp Prevention |
topic |
Colitis Colon carcinogenesis Cyanidin 3-rutinoside Lyophilized açaí pulp Prevention |
description |
This study evaluated the possible protective effects of lyophilized açaí pulp (AP) in a colitis-associated carcinogenesis (CAC) rat model and the modifying effect of cyanidin 3-rutinoside (C3R) on the motility of RKO colon adenocarcinoma cells, using the wound healing assay. Male Wistar rats were induced to develop CAC using 1,2-dimethylhydrazine (DMH) and 2,4,6-trinitrobenzene acid (TNBS). Animals were randomly assigned to different groups that received basal diet or basal diet supplemented with 5.0% or 7.5% lyophilized AP. The findings indicate: 1) C3R (25 μM) has the potential to reduce RKO cell motility in vitro; 2) ingestion of lyophilized AP reduces the total number of aberrant crypt foci (ACF), ACF multiplicity, tumor cell proliferation and incidence of tumors with high grade dysplasia; 3) AP increases the gene expression of negative regulators of cell proliferation such as Dlc1 and Akt3, as well as inflammation (Ppara). Thus, lyophilized AP could exert a potential antitumor activity. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:22:37Z 2018-12-11T17:22:37Z 2018-11-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.fct.2018.08.078 Food and Chemical Toxicology, v. 121, p. 237-245. 1873-6351 0278-6915 http://hdl.handle.net/11449/176819 10.1016/j.fct.2018.08.078 2-s2.0-85053026075 2-s2.0-85053026075.pdf |
url |
http://dx.doi.org/10.1016/j.fct.2018.08.078 http://hdl.handle.net/11449/176819 |
identifier_str_mv |
Food and Chemical Toxicology, v. 121, p. 237-245. 1873-6351 0278-6915 10.1016/j.fct.2018.08.078 2-s2.0-85053026075 2-s2.0-85053026075.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Food and Chemical Toxicology 1,144 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
237-245 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021424698490880 |