Inhibitory effect of cold atmospheric plasma on chronic wound-related multispecies biofilms
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/app11125441 http://hdl.handle.net/11449/229018 |
Resumo: | The presence of microbial biofilms in the wounds affects negatively the healing process and can contribute to therapeutic failures. This study aimed to establish the effective parameters of cold atmospheric plasma (CAP) against wound-related multispecies and monospecies biofilms, and to evaluate the cytotoxicity and genotoxicity of the protocol. Monospecies and multispecies biofilms were formed by methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa and Enterococcus faecalis. The monospecies biofilms were grown in 96 wells plates and multispecies biofilm were formed on collagen membranes. The biofilms were exposed to helium CAP for 1, 3, 5 and 7 min. In monospecies biofilms, the inhibitory effect was detected after 1 min of exposure for E. faecalis and after 3 min for MRSA. A reduction in P. aeruginosa biofilm’s viability was detected after 7 min of exposure. For the multispecies biofilms, the reduction in the overall viability was detected after 5 min of exposure to CAP. Additionally, cytotoxicity and genotoxicity were evaluated by MTT assay and static cytometry, respectively. CAP showed low cytotoxicity and no genotoxicity to mouse fibroblastic cell line (3T3). It could be concluded that He-CAP showed inhibitory effect on wound-related multispecies biofilms, with low cytotoxicity and genotoxicity to mammalian cells. These findings point out the potential application of CAP in wound care. |
id |
UNSP_94517d3e47ec4e2964683d26b1fa05ae |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/229018 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Inhibitory effect of cold atmospheric plasma on chronic wound-related multispecies biofilmsBiofilmPlasma jetWoundsThe presence of microbial biofilms in the wounds affects negatively the healing process and can contribute to therapeutic failures. This study aimed to establish the effective parameters of cold atmospheric plasma (CAP) against wound-related multispecies and monospecies biofilms, and to evaluate the cytotoxicity and genotoxicity of the protocol. Monospecies and multispecies biofilms were formed by methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa and Enterococcus faecalis. The monospecies biofilms were grown in 96 wells plates and multispecies biofilm were formed on collagen membranes. The biofilms were exposed to helium CAP for 1, 3, 5 and 7 min. In monospecies biofilms, the inhibitory effect was detected after 1 min of exposure for E. faecalis and after 3 min for MRSA. A reduction in P. aeruginosa biofilm’s viability was detected after 7 min of exposure. For the multispecies biofilms, the reduction in the overall viability was detected after 5 min of exposure to CAP. Additionally, cytotoxicity and genotoxicity were evaluated by MTT assay and static cytometry, respectively. CAP showed low cytotoxicity and no genotoxicity to mouse fibroblastic cell line (3T3). It could be concluded that He-CAP showed inhibitory effect on wound-related multispecies biofilms, with low cytotoxicity and genotoxicity to mammalian cells. These findings point out the potential application of CAP in wound care.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Institute of Science and Technology São Paulo State University (UNESP)Leibniz Institute for Plasma Science and TechnologyPlasmas and Process Laboratory (LPP) Technological Institute of Aeronautics (ITA)Department of General Pathology University Federal de Minas (UFMG)Department of Physics Guaratinguetá Faculty of Engineering São Paulo State University (UNESP)Department of Environment Engineering Institute of Science & Technology São Paulo State University (UNESP)Institute of Science and Technology São Paulo State University (UNESP)Department of Physics Guaratinguetá Faculty of Engineering São Paulo State University (UNESP)Department of Environment Engineering Institute of Science & Technology São Paulo State University (UNESP)CNPq: 308127/2018-8.CNPq: 405653/2016-6Universidade Estadual Paulista (UNESP)Leibniz Institute for Plasma Science and TechnologyTechnological Institute of Aeronautics (ITA)Universidade Federal de Minas Gerais (UFMG)de Oliveira, Maria Alcionéia Carvalho [UNESP]Lima, Gabriela de Morais Gouvêa [UNESP]Nishime, Thalita M. CastaldelliGontijo, Aline Vidal Lacerda [UNESP]de Menezes, Beatriz Rossi CanutoCaliari, Marcelo VidigalKostov, Konstantin Georgiev [UNESP]Koga-Ito, Cristiane Yumi [UNESP]2022-04-29T08:29:59Z2022-04-29T08:29:59Z2021-06-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/app11125441Applied Sciences (Switzerland), v. 11, n. 12, 2021.2076-3417http://hdl.handle.net/11449/22901810.3390/app111254412-s2.0-85108411251Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengApplied Sciences (Switzerland)info:eu-repo/semantics/openAccess2024-07-01T20:52:16Zoai:repositorio.unesp.br:11449/229018Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:12:55.034824Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Inhibitory effect of cold atmospheric plasma on chronic wound-related multispecies biofilms |
title |
Inhibitory effect of cold atmospheric plasma on chronic wound-related multispecies biofilms |
spellingShingle |
Inhibitory effect of cold atmospheric plasma on chronic wound-related multispecies biofilms de Oliveira, Maria Alcionéia Carvalho [UNESP] Biofilm Plasma jet Wounds |
title_short |
Inhibitory effect of cold atmospheric plasma on chronic wound-related multispecies biofilms |
title_full |
Inhibitory effect of cold atmospheric plasma on chronic wound-related multispecies biofilms |
title_fullStr |
Inhibitory effect of cold atmospheric plasma on chronic wound-related multispecies biofilms |
title_full_unstemmed |
Inhibitory effect of cold atmospheric plasma on chronic wound-related multispecies biofilms |
title_sort |
Inhibitory effect of cold atmospheric plasma on chronic wound-related multispecies biofilms |
author |
de Oliveira, Maria Alcionéia Carvalho [UNESP] |
author_facet |
de Oliveira, Maria Alcionéia Carvalho [UNESP] Lima, Gabriela de Morais Gouvêa [UNESP] Nishime, Thalita M. Castaldelli Gontijo, Aline Vidal Lacerda [UNESP] de Menezes, Beatriz Rossi Canuto Caliari, Marcelo Vidigal Kostov, Konstantin Georgiev [UNESP] Koga-Ito, Cristiane Yumi [UNESP] |
author_role |
author |
author2 |
Lima, Gabriela de Morais Gouvêa [UNESP] Nishime, Thalita M. Castaldelli Gontijo, Aline Vidal Lacerda [UNESP] de Menezes, Beatriz Rossi Canuto Caliari, Marcelo Vidigal Kostov, Konstantin Georgiev [UNESP] Koga-Ito, Cristiane Yumi [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Leibniz Institute for Plasma Science and Technology Technological Institute of Aeronautics (ITA) Universidade Federal de Minas Gerais (UFMG) |
dc.contributor.author.fl_str_mv |
de Oliveira, Maria Alcionéia Carvalho [UNESP] Lima, Gabriela de Morais Gouvêa [UNESP] Nishime, Thalita M. Castaldelli Gontijo, Aline Vidal Lacerda [UNESP] de Menezes, Beatriz Rossi Canuto Caliari, Marcelo Vidigal Kostov, Konstantin Georgiev [UNESP] Koga-Ito, Cristiane Yumi [UNESP] |
dc.subject.por.fl_str_mv |
Biofilm Plasma jet Wounds |
topic |
Biofilm Plasma jet Wounds |
description |
The presence of microbial biofilms in the wounds affects negatively the healing process and can contribute to therapeutic failures. This study aimed to establish the effective parameters of cold atmospheric plasma (CAP) against wound-related multispecies and monospecies biofilms, and to evaluate the cytotoxicity and genotoxicity of the protocol. Monospecies and multispecies biofilms were formed by methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa and Enterococcus faecalis. The monospecies biofilms were grown in 96 wells plates and multispecies biofilm were formed on collagen membranes. The biofilms were exposed to helium CAP for 1, 3, 5 and 7 min. In monospecies biofilms, the inhibitory effect was detected after 1 min of exposure for E. faecalis and after 3 min for MRSA. A reduction in P. aeruginosa biofilm’s viability was detected after 7 min of exposure. For the multispecies biofilms, the reduction in the overall viability was detected after 5 min of exposure to CAP. Additionally, cytotoxicity and genotoxicity were evaluated by MTT assay and static cytometry, respectively. CAP showed low cytotoxicity and no genotoxicity to mouse fibroblastic cell line (3T3). It could be concluded that He-CAP showed inhibitory effect on wound-related multispecies biofilms, with low cytotoxicity and genotoxicity to mammalian cells. These findings point out the potential application of CAP in wound care. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-02 2022-04-29T08:29:59Z 2022-04-29T08:29:59Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/app11125441 Applied Sciences (Switzerland), v. 11, n. 12, 2021. 2076-3417 http://hdl.handle.net/11449/229018 10.3390/app11125441 2-s2.0-85108411251 |
url |
http://dx.doi.org/10.3390/app11125441 http://hdl.handle.net/11449/229018 |
identifier_str_mv |
Applied Sciences (Switzerland), v. 11, n. 12, 2021. 2076-3417 10.3390/app11125441 2-s2.0-85108411251 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Applied Sciences (Switzerland) |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128225429159936 |