The genome sequence of the gram-positive sugarcane pathogen Leifsonia xyli subsp. xyli
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1094/MPMI.2004.17.8.827 http://hdl.handle.net/11449/67815 |
Resumo: | The genome sequence of Leifsonia xyli subsp. xyli, which causes ratoon stunting disease and affects sugarcane worldwide, was determined. The single circular chromosome of Leifsonia xyli subsp. xyli CTCB07 was 2.6 Mb in length with a GC content of 68% and 2,044 predicted open reading frames. The analysis also revealed 307 predicted pseudogenes, which is more than any bacterial plant pathogen sequenced to date. Many of these pseudogenes, if functional, would likely be involved in the degradation of plant heteropolysaccharides, uptake of free sugars, and synthesis of amino acids. Although L. xyli subsp. xyli has only been identified colonizing the xylem vessels of sugarcane, the numbers of predicted regulatory genes and sugar transporters are similar to those in free-living organisms. Some of the predicted pathogenicity genes appear to have been acquired by lateral transfer and include genes for cellulase, pectinase, wilt-inducing protein, lysozyme, and desaturase. The presence of the latter may contribute to stunting, since it is likely involved in the synthesis of abscisic acid, a hormone that arrests growth. Our findings are consistent with the nutritionally fastidious behavior exhibited by L. xyli subsp. xyli and suggest an ongoing adaptation to the restricted ecological niche it inhabits. |
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The genome sequence of the gram-positive sugarcane pathogen Leifsonia xyli subsp. xyliActinomycetalesbacterial genebacterial genomeclassificationDNA base compositiongeneticsmicrobiologymolecular geneticsnucleotide sequencepseudogenesugarcaneBase CompositionGenes, BacterialGenome, BacterialMolecular Sequence DataPseudogenesSaccharumActinobacteria (class)Bacteria (microorganisms)LeifsoniaLeifsonia xyli subsp. xyliPosibacteriaProkaryotaSaccharum hybrid cultivaruncultured actinomyceteThe genome sequence of Leifsonia xyli subsp. xyli, which causes ratoon stunting disease and affects sugarcane worldwide, was determined. The single circular chromosome of Leifsonia xyli subsp. xyli CTCB07 was 2.6 Mb in length with a GC content of 68% and 2,044 predicted open reading frames. The analysis also revealed 307 predicted pseudogenes, which is more than any bacterial plant pathogen sequenced to date. Many of these pseudogenes, if functional, would likely be involved in the degradation of plant heteropolysaccharides, uptake of free sugars, and synthesis of amino acids. Although L. xyli subsp. xyli has only been identified colonizing the xylem vessels of sugarcane, the numbers of predicted regulatory genes and sugar transporters are similar to those in free-living organisms. Some of the predicted pathogenicity genes appear to have been acquired by lateral transfer and include genes for cellulase, pectinase, wilt-inducing protein, lysozyme, and desaturase. The presence of the latter may contribute to stunting, since it is likely involved in the synthesis of abscisic acid, a hormone that arrests growth. Our findings are consistent with the nutritionally fastidious behavior exhibited by L. xyli subsp. xyli and suggest an ongoing adaptation to the restricted ecological niche it inhabits.Esc. Sup. de Agric. Luiz de Queiroz Universidade de São Paulo, Av. Pádua Dias, 11, 13418-900, Piracicaba, SPInstituto de Biociências Universidade de São Paulo, R. do Matão, 277, 05508-900, São Paulo, SPAlellyx Applied Genomics, R. James Clerk Maxwell 320, 13067-850, Campinas, SPEmbrapa Recurs. Geneticos B., 70770-900 Brasília-DFInstituto Biológico, Av. C. Rodrigues Alves, 1252, 04014-002, São Paulo, SPFaculdade Cie. Agrarias Veterinarias Universidade Estadual Paulista, Av. Prof. Paulo D, Castellane s/n, 14884-900, Jaboticabal, SPDept. of Microbiology University of Washington HSB, Seattle, WA 98195-7242Ctro. de Citricultura Sylvio Moreira, 13490-000, Cordeirópolis, SPInstituto de Química Universidade de São Paulo, Av. Prof. Lineu Prestes, 748, 05508-900, São Paulo, SPFaculdade de Cie. Agronômicas Universidade Estadual Paulista, C. P. 237, 18603-970, Botucatu, SPInst. Ludwig Pesquisa Sobre O Cancer, Rua Prof. Antonio Prudente, 109, 01509-010, São Paulo, SPCtro. de Cie. Exatas e Tecnologia Univ. Federal do Mato Grosso do Sul, C. P. 549, 79070-900, Campo Grande, MSCtro. de Ciê. Agrárias Univ. Federal de São Carlos, C. P. 153, 13600-970, Araras, SPFaculdade de Filosofia Ciências e Letras Universidade de São Paulo, Av. Bandeirantes, 3900, 14040-901, Ribeirão Preto, SPFaculdade Cie. Farmaceuticas R. Universidade de São Paulo, Av. do Café S/N, 14040-903, Ribeirão Preto, SPInst. de Ciê. Bioméd. Universidade de São Paulo, Av. Prof. Lineu Prestes, 1374, 05508-900, São Paulo, SPInstituto de Biociências Universidade Estadual Paulista, 18618-000, Botucatu, SPNucleo Integrado de Biotecnologia Universidade de Mogi Das Cruzes, Av. Dr. C. Xavier Almeida Souza, 200, 08780-911, Mogi das Cruzes, SPInstituto de Biociências Universidade Estadual de Campinas, C. P. 6109, 13083-970, Campinas, SPInst. Agronômico de Campinas, C. P. 28, 13001-970, Campinas, SPCtro. de Ener. Nucl. na Agricultura Universidade de São Paulo, C. P. 96, 13400-970, Piracicaba, SPLudwig Institute for Cancer Research, 605 Third Avenue, New York, NY 10158BSES Limited, 50 Meiers Road, Indooroopilly, QLD 4068CRC for Tropical Plant Protection University of Queensland, St. Lucia, QLD 4072Instituto de Computação Universidade Estadual de Campinas, C.P. 6176, Campinas, SP, 13084-971Faculdade Cie. Agrarias Veterinarias Universidade Estadual Paulista, Av. Prof. Paulo D, Castellane s/n, 14884-900, Jaboticabal, SPFaculdade de Cie. Agronômicas Universidade Estadual Paulista, C. P. 237, 18603-970, Botucatu, SPInstituto de Biociências Universidade Estadual Paulista, 18618-000, Botucatu, SPUniversidade de São Paulo (USP)Alellyx Applied GenomicsEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Instituto BiológicoUniversidade Estadual Paulista (Unesp)HSBCtro. de Citricultura Sylvio MoreiraInst. Ludwig Pesquisa Sobre O CancerUniv. Federal do Mato Grosso do SulUniversidade Federal de São Carlos (UFSCar)Universidade de Mogi Das CruzesUniversidade Estadual de Campinas (UNICAMP)Inst. Agronômico de CampinasLudwig Institute for Cancer ResearchBSES LimitedUniversity of QueenslandMonteiro-Vitorello, Claudia B.Camargo, Luis E. A.Van Sluys, Marie A.Kitajima, João P.Truffi, DanielaDo Amaral, Alexandre M.Harakava, RicardoDe Oliveira, Julio C. F.Wood, DerekDe Oliveira, Mariana C.Miyaki, CristinaTakita, Marco A.Da Silva, Ana C. R.Furlan, Luis R. [UNESP]Carraro, Dirce M.Camarotte, GiovanaAlmeida Jr., Nalvo F.Carrer, HelaineCoutinho, Luiz L.El-Dorry, Hamza A.Ferro, Maria I. T. [UNESP]Gagliardi, Paulo R.Giglioti, EderGoldman, Maria H. S.Goldman, Gustavo H.Kimura, Edna T.Ferro, Emer S.Kuramae, Eiko E. [UNESP]Lemos, Eliana G. M. [UNESP]Lemos, Manoel V. F. [UNESP]Mauro, Sonia M. Z. [UNESP]Machado, Marcos A.Marino, Celso L. [UNESP]Menck, Carlos F.Nunes, Luiz R.Oliveira, Regina C.Pereira, Gonçalo G.Siqueira, WalterDe Souza, Alessandra A.Tsai, Siu M.Zanca, A. S.Simpson, Andrew J. G.Brumbley, Stevens M.Setúbal, João C.2014-05-27T11:21:07Z2014-05-27T11:21:07Z2004-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article827-836application/pdfhttp://dx.doi.org/10.1094/MPMI.2004.17.8.827Molecular Plant-Microbe Interactions, v. 17, n. 8, p. 827-836, 2004.0894-0282http://hdl.handle.net/11449/6781510.1094/MPMI.2004.17.8.827WOS:0002227312000012-s2.0-32426908782-s2.0-3242690878.pdf01653487382083190000-0003-4524-954XScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMolecular Plant-Microbe Interactions3.5881,935info:eu-repo/semantics/openAccess2024-06-07T15:32:35Zoai:repositorio.unesp.br:11449/67815Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:07:17.368425Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The genome sequence of the gram-positive sugarcane pathogen Leifsonia xyli subsp. xyli |
title |
The genome sequence of the gram-positive sugarcane pathogen Leifsonia xyli subsp. xyli |
spellingShingle |
The genome sequence of the gram-positive sugarcane pathogen Leifsonia xyli subsp. xyli Monteiro-Vitorello, Claudia B. Actinomycetales bacterial gene bacterial genome classification DNA base composition genetics microbiology molecular genetics nucleotide sequence pseudogene sugarcane Base Composition Genes, Bacterial Genome, Bacterial Molecular Sequence Data Pseudogenes Saccharum Actinobacteria (class) Bacteria (microorganisms) Leifsonia Leifsonia xyli subsp. xyli Posibacteria Prokaryota Saccharum hybrid cultivar uncultured actinomycete |
title_short |
The genome sequence of the gram-positive sugarcane pathogen Leifsonia xyli subsp. xyli |
title_full |
The genome sequence of the gram-positive sugarcane pathogen Leifsonia xyli subsp. xyli |
title_fullStr |
The genome sequence of the gram-positive sugarcane pathogen Leifsonia xyli subsp. xyli |
title_full_unstemmed |
The genome sequence of the gram-positive sugarcane pathogen Leifsonia xyli subsp. xyli |
title_sort |
The genome sequence of the gram-positive sugarcane pathogen Leifsonia xyli subsp. xyli |
author |
Monteiro-Vitorello, Claudia B. |
author_facet |
Monteiro-Vitorello, Claudia B. Camargo, Luis E. A. Van Sluys, Marie A. Kitajima, João P. Truffi, Daniela Do Amaral, Alexandre M. Harakava, Ricardo De Oliveira, Julio C. F. Wood, Derek De Oliveira, Mariana C. Miyaki, Cristina Takita, Marco A. Da Silva, Ana C. R. Furlan, Luis R. [UNESP] Carraro, Dirce M. Camarotte, Giovana Almeida Jr., Nalvo F. Carrer, Helaine Coutinho, Luiz L. El-Dorry, Hamza A. Ferro, Maria I. T. [UNESP] Gagliardi, Paulo R. Giglioti, Eder Goldman, Maria H. S. Goldman, Gustavo H. Kimura, Edna T. Ferro, Emer S. Kuramae, Eiko E. [UNESP] Lemos, Eliana G. M. [UNESP] Lemos, Manoel V. F. [UNESP] Mauro, Sonia M. Z. [UNESP] Machado, Marcos A. Marino, Celso L. [UNESP] Menck, Carlos F. Nunes, Luiz R. Oliveira, Regina C. Pereira, Gonçalo G. Siqueira, Walter De Souza, Alessandra A. Tsai, Siu M. Zanca, A. S. Simpson, Andrew J. G. Brumbley, Stevens M. Setúbal, João C. |
author_role |
author |
author2 |
Camargo, Luis E. A. Van Sluys, Marie A. Kitajima, João P. Truffi, Daniela Do Amaral, Alexandre M. Harakava, Ricardo De Oliveira, Julio C. F. Wood, Derek De Oliveira, Mariana C. Miyaki, Cristina Takita, Marco A. Da Silva, Ana C. R. Furlan, Luis R. [UNESP] Carraro, Dirce M. Camarotte, Giovana Almeida Jr., Nalvo F. Carrer, Helaine Coutinho, Luiz L. El-Dorry, Hamza A. Ferro, Maria I. T. [UNESP] Gagliardi, Paulo R. Giglioti, Eder Goldman, Maria H. S. Goldman, Gustavo H. Kimura, Edna T. Ferro, Emer S. Kuramae, Eiko E. [UNESP] Lemos, Eliana G. M. [UNESP] Lemos, Manoel V. F. [UNESP] Mauro, Sonia M. Z. [UNESP] Machado, Marcos A. Marino, Celso L. [UNESP] Menck, Carlos F. Nunes, Luiz R. Oliveira, Regina C. Pereira, Gonçalo G. Siqueira, Walter De Souza, Alessandra A. Tsai, Siu M. Zanca, A. S. Simpson, Andrew J. G. Brumbley, Stevens M. Setúbal, João C. |
author2_role |
author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Alellyx Applied Genomics Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Instituto Biológico Universidade Estadual Paulista (Unesp) HSB Ctro. de Citricultura Sylvio Moreira Inst. Ludwig Pesquisa Sobre O Cancer Univ. Federal do Mato Grosso do Sul Universidade Federal de São Carlos (UFSCar) Universidade de Mogi Das Cruzes Universidade Estadual de Campinas (UNICAMP) Inst. Agronômico de Campinas Ludwig Institute for Cancer Research BSES Limited University of Queensland |
dc.contributor.author.fl_str_mv |
Monteiro-Vitorello, Claudia B. Camargo, Luis E. A. Van Sluys, Marie A. Kitajima, João P. Truffi, Daniela Do Amaral, Alexandre M. Harakava, Ricardo De Oliveira, Julio C. F. Wood, Derek De Oliveira, Mariana C. Miyaki, Cristina Takita, Marco A. Da Silva, Ana C. R. Furlan, Luis R. [UNESP] Carraro, Dirce M. Camarotte, Giovana Almeida Jr., Nalvo F. Carrer, Helaine Coutinho, Luiz L. El-Dorry, Hamza A. Ferro, Maria I. T. [UNESP] Gagliardi, Paulo R. Giglioti, Eder Goldman, Maria H. S. Goldman, Gustavo H. Kimura, Edna T. Ferro, Emer S. Kuramae, Eiko E. [UNESP] Lemos, Eliana G. M. [UNESP] Lemos, Manoel V. F. [UNESP] Mauro, Sonia M. Z. [UNESP] Machado, Marcos A. Marino, Celso L. [UNESP] Menck, Carlos F. Nunes, Luiz R. Oliveira, Regina C. Pereira, Gonçalo G. Siqueira, Walter De Souza, Alessandra A. Tsai, Siu M. Zanca, A. S. Simpson, Andrew J. G. Brumbley, Stevens M. Setúbal, João C. |
dc.subject.por.fl_str_mv |
Actinomycetales bacterial gene bacterial genome classification DNA base composition genetics microbiology molecular genetics nucleotide sequence pseudogene sugarcane Base Composition Genes, Bacterial Genome, Bacterial Molecular Sequence Data Pseudogenes Saccharum Actinobacteria (class) Bacteria (microorganisms) Leifsonia Leifsonia xyli subsp. xyli Posibacteria Prokaryota Saccharum hybrid cultivar uncultured actinomycete |
topic |
Actinomycetales bacterial gene bacterial genome classification DNA base composition genetics microbiology molecular genetics nucleotide sequence pseudogene sugarcane Base Composition Genes, Bacterial Genome, Bacterial Molecular Sequence Data Pseudogenes Saccharum Actinobacteria (class) Bacteria (microorganisms) Leifsonia Leifsonia xyli subsp. xyli Posibacteria Prokaryota Saccharum hybrid cultivar uncultured actinomycete |
description |
The genome sequence of Leifsonia xyli subsp. xyli, which causes ratoon stunting disease and affects sugarcane worldwide, was determined. The single circular chromosome of Leifsonia xyli subsp. xyli CTCB07 was 2.6 Mb in length with a GC content of 68% and 2,044 predicted open reading frames. The analysis also revealed 307 predicted pseudogenes, which is more than any bacterial plant pathogen sequenced to date. Many of these pseudogenes, if functional, would likely be involved in the degradation of plant heteropolysaccharides, uptake of free sugars, and synthesis of amino acids. Although L. xyli subsp. xyli has only been identified colonizing the xylem vessels of sugarcane, the numbers of predicted regulatory genes and sugar transporters are similar to those in free-living organisms. Some of the predicted pathogenicity genes appear to have been acquired by lateral transfer and include genes for cellulase, pectinase, wilt-inducing protein, lysozyme, and desaturase. The presence of the latter may contribute to stunting, since it is likely involved in the synthesis of abscisic acid, a hormone that arrests growth. Our findings are consistent with the nutritionally fastidious behavior exhibited by L. xyli subsp. xyli and suggest an ongoing adaptation to the restricted ecological niche it inhabits. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-08-01 2014-05-27T11:21:07Z 2014-05-27T11:21:07Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1094/MPMI.2004.17.8.827 Molecular Plant-Microbe Interactions, v. 17, n. 8, p. 827-836, 2004. 0894-0282 http://hdl.handle.net/11449/67815 10.1094/MPMI.2004.17.8.827 WOS:000222731200001 2-s2.0-3242690878 2-s2.0-3242690878.pdf 0165348738208319 0000-0003-4524-954X |
url |
http://dx.doi.org/10.1094/MPMI.2004.17.8.827 http://hdl.handle.net/11449/67815 |
identifier_str_mv |
Molecular Plant-Microbe Interactions, v. 17, n. 8, p. 827-836, 2004. 0894-0282 10.1094/MPMI.2004.17.8.827 WOS:000222731200001 2-s2.0-3242690878 2-s2.0-3242690878.pdf 0165348738208319 0000-0003-4524-954X |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Molecular Plant-Microbe Interactions 3.588 1,935 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
827-836 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129393960157184 |