Structure, organization, and expression of the alpha prolamin multigenic family bring new insights into the evolutionary relationships among grasses
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | https://dl.sciencesocieties.org/publications/tpg/abstracts/8/1/plantgenome2014.06.0027 http://hdl.handle.net/11449/129172 |
Resumo: | Prolamins are the major seed storage proteins of grasses. In maize and related species, prolamins are classified into alpha-, beta-, gamma-, and delta-subclasses by their solubility properties. alpha-prolamins are encoded by multigene families and have a secondary structure that consists of tandem alpha-helix repeats. Maize has two alpha-prolamin subclasses, namely the 19 and 22 kDa subclasses that contain nine and 10 alpha-helix repeats, respectively. Here, we present an evolutionary study based on the structure, organization, and expression of alpha-prolamins in maize, sugarcane, sorghum, and coix. True 22 kDa subclasses containing 10 repeats are conserved in all four species, but true 19 kDa subclasses containing nine repeats are found only in maize and sugarcane. We discovered a 19 kD alpha-like a-coixin that, as in sorghum, is encoded by few genes. These data suggest that a 19 kDa progenitor present in the ancestor common to maize, coix, sorghum, and sugarcane was preserved at low copy number in coix and sorghum, while amplified into multigene family architecture in maize and sugarcane. The expression profiling of alpha-prolamins, verified by two-dimensional gels, showed highly conserved multispot composition for the 19 kDa alpha-prolamins in maize and sugarcane. Coix and sorghum did not present true 19 kDa alpha-prolamin spots. Our data show remarkable similarity between maize and sugarcane 19 kDa alpha-prolamins regarding both gene structure and expression. Since the multigene architecture of 19 kDa alpha-canein appeared after sugarcane diverged from sorghum, our data suggest that maize and sugarcane might have acquired the multigene family encoding these storage proteins from a common ancestor. |
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Structure, organization, and expression of the alpha prolamin multigenic family bring new insights into the evolutionary relationships among grassesProlamins are the major seed storage proteins of grasses. In maize and related species, prolamins are classified into alpha-, beta-, gamma-, and delta-subclasses by their solubility properties. alpha-prolamins are encoded by multigene families and have a secondary structure that consists of tandem alpha-helix repeats. Maize has two alpha-prolamin subclasses, namely the 19 and 22 kDa subclasses that contain nine and 10 alpha-helix repeats, respectively. Here, we present an evolutionary study based on the structure, organization, and expression of alpha-prolamins in maize, sugarcane, sorghum, and coix. True 22 kDa subclasses containing 10 repeats are conserved in all four species, but true 19 kDa subclasses containing nine repeats are found only in maize and sugarcane. We discovered a 19 kD alpha-like a-coixin that, as in sorghum, is encoded by few genes. These data suggest that a 19 kDa progenitor present in the ancestor common to maize, coix, sorghum, and sugarcane was preserved at low copy number in coix and sorghum, while amplified into multigene family architecture in maize and sugarcane. The expression profiling of alpha-prolamins, verified by two-dimensional gels, showed highly conserved multispot composition for the 19 kDa alpha-prolamins in maize and sugarcane. Coix and sorghum did not present true 19 kDa alpha-prolamin spots. Our data show remarkable similarity between maize and sugarcane 19 kDa alpha-prolamins regarding both gene structure and expression. Since the multigene architecture of 19 kDa alpha-canein appeared after sugarcane diverged from sorghum, our data suggest that maize and sugarcane might have acquired the multigene family encoding these storage proteins from a common ancestor.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Estadual de Campinas (UNICAMP), Departamento de Genética e Evolução, Instituto de Biologia, 13083-875, Campinas, SP, BrazilUniversidade Estadual Paulista (UNESP), Departamento de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias, 14884-900, Jaboticabal, SP, BrazilCentro de Biologia Molecular e Engenharia GenéticaUniversidade Estadual Paulista (UNESP), Departamento de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias, 14884-900, Jaboticabal, SP, BrazilFAPESP: 2011/12431-0FAPESP: 2011/11650-0Crop Science Soc AmerUniversidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (Unesp)Correa de Souza, Rafael SoaresBalbuena, Tiago Santana [UNESP]Arruda, Paulo2015-10-21T20:30:33Z2015-10-21T20:30:33Z2015-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-11https://dl.sciencesocieties.org/publications/tpg/abstracts/8/1/plantgenome2014.06.0027Plant Genome. Madison: Crop Science Soc Amer, v. 8, n. 1, 1-11 p., 2015.1940-3372http://hdl.handle.net/11449/12917210.3835/plantgenome2014.06.0027WOS:0003524949000032767178161293127Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPlant Genome2.9231,728info:eu-repo/semantics/openAccess2024-06-07T15:32:21Zoai:repositorio.unesp.br:11449/129172Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:54:36.013623Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Structure, organization, and expression of the alpha prolamin multigenic family bring new insights into the evolutionary relationships among grasses |
title |
Structure, organization, and expression of the alpha prolamin multigenic family bring new insights into the evolutionary relationships among grasses |
spellingShingle |
Structure, organization, and expression of the alpha prolamin multigenic family bring new insights into the evolutionary relationships among grasses Correa de Souza, Rafael Soares |
title_short |
Structure, organization, and expression of the alpha prolamin multigenic family bring new insights into the evolutionary relationships among grasses |
title_full |
Structure, organization, and expression of the alpha prolamin multigenic family bring new insights into the evolutionary relationships among grasses |
title_fullStr |
Structure, organization, and expression of the alpha prolamin multigenic family bring new insights into the evolutionary relationships among grasses |
title_full_unstemmed |
Structure, organization, and expression of the alpha prolamin multigenic family bring new insights into the evolutionary relationships among grasses |
title_sort |
Structure, organization, and expression of the alpha prolamin multigenic family bring new insights into the evolutionary relationships among grasses |
author |
Correa de Souza, Rafael Soares |
author_facet |
Correa de Souza, Rafael Soares Balbuena, Tiago Santana [UNESP] Arruda, Paulo |
author_role |
author |
author2 |
Balbuena, Tiago Santana [UNESP] Arruda, Paulo |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Correa de Souza, Rafael Soares Balbuena, Tiago Santana [UNESP] Arruda, Paulo |
description |
Prolamins are the major seed storage proteins of grasses. In maize and related species, prolamins are classified into alpha-, beta-, gamma-, and delta-subclasses by their solubility properties. alpha-prolamins are encoded by multigene families and have a secondary structure that consists of tandem alpha-helix repeats. Maize has two alpha-prolamin subclasses, namely the 19 and 22 kDa subclasses that contain nine and 10 alpha-helix repeats, respectively. Here, we present an evolutionary study based on the structure, organization, and expression of alpha-prolamins in maize, sugarcane, sorghum, and coix. True 22 kDa subclasses containing 10 repeats are conserved in all four species, but true 19 kDa subclasses containing nine repeats are found only in maize and sugarcane. We discovered a 19 kD alpha-like a-coixin that, as in sorghum, is encoded by few genes. These data suggest that a 19 kDa progenitor present in the ancestor common to maize, coix, sorghum, and sugarcane was preserved at low copy number in coix and sorghum, while amplified into multigene family architecture in maize and sugarcane. The expression profiling of alpha-prolamins, verified by two-dimensional gels, showed highly conserved multispot composition for the 19 kDa alpha-prolamins in maize and sugarcane. Coix and sorghum did not present true 19 kDa alpha-prolamin spots. Our data show remarkable similarity between maize and sugarcane 19 kDa alpha-prolamins regarding both gene structure and expression. Since the multigene architecture of 19 kDa alpha-canein appeared after sugarcane diverged from sorghum, our data suggest that maize and sugarcane might have acquired the multigene family encoding these storage proteins from a common ancestor. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-10-21T20:30:33Z 2015-10-21T20:30:33Z 2015-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://dl.sciencesocieties.org/publications/tpg/abstracts/8/1/plantgenome2014.06.0027 Plant Genome. Madison: Crop Science Soc Amer, v. 8, n. 1, 1-11 p., 2015. 1940-3372 http://hdl.handle.net/11449/129172 10.3835/plantgenome2014.06.0027 WOS:000352494900003 2767178161293127 |
url |
https://dl.sciencesocieties.org/publications/tpg/abstracts/8/1/plantgenome2014.06.0027 http://hdl.handle.net/11449/129172 |
identifier_str_mv |
Plant Genome. Madison: Crop Science Soc Amer, v. 8, n. 1, 1-11 p., 2015. 1940-3372 10.3835/plantgenome2014.06.0027 WOS:000352494900003 2767178161293127 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Plant Genome 2.923 1,728 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-11 |
dc.publisher.none.fl_str_mv |
Crop Science Soc Amer |
publisher.none.fl_str_mv |
Crop Science Soc Amer |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129262513815552 |