Structure, organization, and expression of the alpha prolamin multigenic family bring new insights into the evolutionary relationships among grasses

Detalhes bibliográficos
Autor(a) principal: Correa de Souza, Rafael Soares
Data de Publicação: 2015
Outros Autores: Balbuena, Tiago Santana [UNESP], Arruda, Paulo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: https://dl.sciencesocieties.org/publications/tpg/abstracts/8/1/plantgenome2014.06.0027
http://hdl.handle.net/11449/129172
Resumo: Prolamins are the major seed storage proteins of grasses. In maize and related species, prolamins are classified into alpha-, beta-, gamma-, and delta-subclasses by their solubility properties. alpha-prolamins are encoded by multigene families and have a secondary structure that consists of tandem alpha-helix repeats. Maize has two alpha-prolamin subclasses, namely the 19 and 22 kDa subclasses that contain nine and 10 alpha-helix repeats, respectively. Here, we present an evolutionary study based on the structure, organization, and expression of alpha-prolamins in maize, sugarcane, sorghum, and coix. True 22 kDa subclasses containing 10 repeats are conserved in all four species, but true 19 kDa subclasses containing nine repeats are found only in maize and sugarcane. We discovered a 19 kD alpha-like a-coixin that, as in sorghum, is encoded by few genes. These data suggest that a 19 kDa progenitor present in the ancestor common to maize, coix, sorghum, and sugarcane was preserved at low copy number in coix and sorghum, while amplified into multigene family architecture in maize and sugarcane. The expression profiling of alpha-prolamins, verified by two-dimensional gels, showed highly conserved multispot composition for the 19 kDa alpha-prolamins in maize and sugarcane. Coix and sorghum did not present true 19 kDa alpha-prolamin spots. Our data show remarkable similarity between maize and sugarcane 19 kDa alpha-prolamins regarding both gene structure and expression. Since the multigene architecture of 19 kDa alpha-canein appeared after sugarcane diverged from sorghum, our data suggest that maize and sugarcane might have acquired the multigene family encoding these storage proteins from a common ancestor.
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spelling Structure, organization, and expression of the alpha prolamin multigenic family bring new insights into the evolutionary relationships among grassesProlamins are the major seed storage proteins of grasses. In maize and related species, prolamins are classified into alpha-, beta-, gamma-, and delta-subclasses by their solubility properties. alpha-prolamins are encoded by multigene families and have a secondary structure that consists of tandem alpha-helix repeats. Maize has two alpha-prolamin subclasses, namely the 19 and 22 kDa subclasses that contain nine and 10 alpha-helix repeats, respectively. Here, we present an evolutionary study based on the structure, organization, and expression of alpha-prolamins in maize, sugarcane, sorghum, and coix. True 22 kDa subclasses containing 10 repeats are conserved in all four species, but true 19 kDa subclasses containing nine repeats are found only in maize and sugarcane. We discovered a 19 kD alpha-like a-coixin that, as in sorghum, is encoded by few genes. These data suggest that a 19 kDa progenitor present in the ancestor common to maize, coix, sorghum, and sugarcane was preserved at low copy number in coix and sorghum, while amplified into multigene family architecture in maize and sugarcane. The expression profiling of alpha-prolamins, verified by two-dimensional gels, showed highly conserved multispot composition for the 19 kDa alpha-prolamins in maize and sugarcane. Coix and sorghum did not present true 19 kDa alpha-prolamin spots. Our data show remarkable similarity between maize and sugarcane 19 kDa alpha-prolamins regarding both gene structure and expression. Since the multigene architecture of 19 kDa alpha-canein appeared after sugarcane diverged from sorghum, our data suggest that maize and sugarcane might have acquired the multigene family encoding these storage proteins from a common ancestor.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Estadual de Campinas (UNICAMP), Departamento de Genética e Evolução, Instituto de Biologia, 13083-875, Campinas, SP, BrazilUniversidade Estadual Paulista (UNESP), Departamento de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias, 14884-900, Jaboticabal, SP, BrazilCentro de Biologia Molecular e Engenharia GenéticaUniversidade Estadual Paulista (UNESP), Departamento de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias, 14884-900, Jaboticabal, SP, BrazilFAPESP: 2011/12431-0FAPESP: 2011/11650-0Crop Science Soc AmerUniversidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (Unesp)Correa de Souza, Rafael SoaresBalbuena, Tiago Santana [UNESP]Arruda, Paulo2015-10-21T20:30:33Z2015-10-21T20:30:33Z2015-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-11https://dl.sciencesocieties.org/publications/tpg/abstracts/8/1/plantgenome2014.06.0027Plant Genome. Madison: Crop Science Soc Amer, v. 8, n. 1, 1-11 p., 2015.1940-3372http://hdl.handle.net/11449/12917210.3835/plantgenome2014.06.0027WOS:0003524949000032767178161293127Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPlant Genome2.9231,728info:eu-repo/semantics/openAccess2024-06-07T15:32:21Zoai:repositorio.unesp.br:11449/129172Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:54:36.013623Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Structure, organization, and expression of the alpha prolamin multigenic family bring new insights into the evolutionary relationships among grasses
title Structure, organization, and expression of the alpha prolamin multigenic family bring new insights into the evolutionary relationships among grasses
spellingShingle Structure, organization, and expression of the alpha prolamin multigenic family bring new insights into the evolutionary relationships among grasses
Correa de Souza, Rafael Soares
title_short Structure, organization, and expression of the alpha prolamin multigenic family bring new insights into the evolutionary relationships among grasses
title_full Structure, organization, and expression of the alpha prolamin multigenic family bring new insights into the evolutionary relationships among grasses
title_fullStr Structure, organization, and expression of the alpha prolamin multigenic family bring new insights into the evolutionary relationships among grasses
title_full_unstemmed Structure, organization, and expression of the alpha prolamin multigenic family bring new insights into the evolutionary relationships among grasses
title_sort Structure, organization, and expression of the alpha prolamin multigenic family bring new insights into the evolutionary relationships among grasses
author Correa de Souza, Rafael Soares
author_facet Correa de Souza, Rafael Soares
Balbuena, Tiago Santana [UNESP]
Arruda, Paulo
author_role author
author2 Balbuena, Tiago Santana [UNESP]
Arruda, Paulo
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Campinas (UNICAMP)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Correa de Souza, Rafael Soares
Balbuena, Tiago Santana [UNESP]
Arruda, Paulo
description Prolamins are the major seed storage proteins of grasses. In maize and related species, prolamins are classified into alpha-, beta-, gamma-, and delta-subclasses by their solubility properties. alpha-prolamins are encoded by multigene families and have a secondary structure that consists of tandem alpha-helix repeats. Maize has two alpha-prolamin subclasses, namely the 19 and 22 kDa subclasses that contain nine and 10 alpha-helix repeats, respectively. Here, we present an evolutionary study based on the structure, organization, and expression of alpha-prolamins in maize, sugarcane, sorghum, and coix. True 22 kDa subclasses containing 10 repeats are conserved in all four species, but true 19 kDa subclasses containing nine repeats are found only in maize and sugarcane. We discovered a 19 kD alpha-like a-coixin that, as in sorghum, is encoded by few genes. These data suggest that a 19 kDa progenitor present in the ancestor common to maize, coix, sorghum, and sugarcane was preserved at low copy number in coix and sorghum, while amplified into multigene family architecture in maize and sugarcane. The expression profiling of alpha-prolamins, verified by two-dimensional gels, showed highly conserved multispot composition for the 19 kDa alpha-prolamins in maize and sugarcane. Coix and sorghum did not present true 19 kDa alpha-prolamin spots. Our data show remarkable similarity between maize and sugarcane 19 kDa alpha-prolamins regarding both gene structure and expression. Since the multigene architecture of 19 kDa alpha-canein appeared after sugarcane diverged from sorghum, our data suggest that maize and sugarcane might have acquired the multigene family encoding these storage proteins from a common ancestor.
publishDate 2015
dc.date.none.fl_str_mv 2015-10-21T20:30:33Z
2015-10-21T20:30:33Z
2015-03-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://dl.sciencesocieties.org/publications/tpg/abstracts/8/1/plantgenome2014.06.0027
Plant Genome. Madison: Crop Science Soc Amer, v. 8, n. 1, 1-11 p., 2015.
1940-3372
http://hdl.handle.net/11449/129172
10.3835/plantgenome2014.06.0027
WOS:000352494900003
2767178161293127
url https://dl.sciencesocieties.org/publications/tpg/abstracts/8/1/plantgenome2014.06.0027
http://hdl.handle.net/11449/129172
identifier_str_mv Plant Genome. Madison: Crop Science Soc Amer, v. 8, n. 1, 1-11 p., 2015.
1940-3372
10.3835/plantgenome2014.06.0027
WOS:000352494900003
2767178161293127
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Plant Genome
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dc.publisher.none.fl_str_mv Crop Science Soc Amer
publisher.none.fl_str_mv Crop Science Soc Amer
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
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instname_str Universidade Estadual Paulista (UNESP)
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