Aspectos histológicos da união da enxertia hipocotiledonar do maracujazeiro-amarelo

• Autor(a) principal: Filho, Geraldo Costa Nogueira
• Data de Publicação: 2010
• Outros Autores: Roncatto, Givanildo, Ruggiero, Carlos [UNESP], de Oliveira, João Carlos, Malheiros, Euclides Braga, Filho, Carlos Ferreira Damião
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/231960
• topic: Histology; Passiflora spp.; Photomicrography; Rootstock; Vegetative propagation

The objective of this work was to observe, one month after the hypocotyledonary grafting, the connection between the vascular tissues of the graft, the rootstock and anatomical differences in the formation of the graft union among the seven rootstocks. Samples were collected from the region of grafting of two seedlings from each graft/rootstock combination. The regions of the graft were fixed, dehydrated in alcohol series, embedded in paraffin, blocks processing and sectioned in microtome. The sections obtained were mounted on glass slides, disparafinned, stained with Safranin and dried again, resulting in permanent histological slides. Approximately 800 sections were observed and analyzed by optical microscope and photomicrographed. There was anatomical difference in the formation of the graft union among the seven rootstocks, and one month after, the connection between the graft and vascular tissues of the rootstock was observable.