Differential Expression of ADAM23, CDKN2A (P16), MMP14 and VIM Associated with Giant Cell Tumor of Bone
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.7150/jca.11238 http://hdl.handle.net/11449/131423 |
Resumo: | Though benign, giant cell tumor of bone (GCTB) can become aggressive and can exhibit a high mitotic rate, necrosis and rarely vascular invasion and metastasis. GCTB has unique histologic characteristics, a high rate of multinucleated cells, a variable and unpredictable growth potential and uncertain biological behavior. In this study, we sought to identify genes differentially expressed in GCTB, thus building a molecular profile of this tumor. We performed quantitative real-time polymerase chain reaction (qPCR), immunohistochemistry and analyses of methylation to identify genes that are putatively associated with GCTB. The expression of the ADAM23 and CDKN2A genes was decreased in GCTB samples compared to normal bone tissue, measured by qPCR. Additionally, a high hypermethylation frequency of the promoter regions of ADAM23 and CDKN2A in GCTB was observed. The expression of the MAP2K3, MMP14, TIMP2 and VIM genes was significantly higher in GCTB than in normal bone tissue, a fact that was confirmed by qPCR and immunohistochemistry. The set of genes identified here furthers our understanding of the molecular basis of GCTB. |
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Differential Expression of ADAM23, CDKN2A (P16), MMP14 and VIM Associated with Giant Cell Tumor of BoneGiant cell tumor of boneGene expressionHypermethylationImmunohistochemistryThough benign, giant cell tumor of bone (GCTB) can become aggressive and can exhibit a high mitotic rate, necrosis and rarely vascular invasion and metastasis. GCTB has unique histologic characteristics, a high rate of multinucleated cells, a variable and unpredictable growth potential and uncertain biological behavior. In this study, we sought to identify genes differentially expressed in GCTB, thus building a molecular profile of this tumor. We performed quantitative real-time polymerase chain reaction (qPCR), immunohistochemistry and analyses of methylation to identify genes that are putatively associated with GCTB. The expression of the ADAM23 and CDKN2A genes was decreased in GCTB samples compared to normal bone tissue, measured by qPCR. Additionally, a high hypermethylation frequency of the promoter regions of ADAM23 and CDKN2A in GCTB was observed. The expression of the MAP2K3, MMP14, TIMP2 and VIM genes was significantly higher in GCTB than in normal bone tissue, a fact that was confirmed by qPCR and immunohistochemistry. The set of genes identified here furthers our understanding of the molecular basis of GCTB.Laboratory of Genomics Studies, UNESP, São José do Rio Preto, Brazil.Center for the Study of Cancer Prognosis, FAMERP, São José do Rio Preto, Brazil.Department of Pathology, FAMERP, São José do Rio Preto, Brazil.Department of Epidemiology and Collective Health, FAMERP, São José do Rio Preto, Brazil.Laboratory of Genomics Studies, UNESP, São José do Rio Preto, Brazil.Journal Of CancerUniversidade Estadual Paulista (Unesp)Faculdade de Medicina de São José do Rio Preto (FAMERP)Conceição, André Luis Giacometti [UNESP]Babeto, Erica [UNESP]Candido, Natalia Maria [UNESP]Franco, Fernanda Craveiro [UNESP]Zuccari, Débora Aparecida Pires de CamposBonilha, Jane LopesCordeiro, José AntônioCalmon, Marilia Freitas [UNESP]Rahal, Paula [UNESP]2015-12-07T15:35:15Z2015-12-07T15:35:15Z2015info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article593-603application/pdfhttp://dx.doi.org/10.7150/jca.11238Journal Of Cancer, v. 6, n. 7, p. 593-603, 2015.1837-9664http://hdl.handle.net/11449/13142310.7150/jca.11238PMC4466407.pdf799108236267121226078788PMC44664070000-0001-5693-6148PubMedreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Cancer3.2491,159info:eu-repo/semantics/openAccess2023-11-20T06:12:56Zoai:repositorio.unesp.br:11449/131423Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-20T06:12:56Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Differential Expression of ADAM23, CDKN2A (P16), MMP14 and VIM Associated with Giant Cell Tumor of Bone |
title |
Differential Expression of ADAM23, CDKN2A (P16), MMP14 and VIM Associated with Giant Cell Tumor of Bone |
spellingShingle |
Differential Expression of ADAM23, CDKN2A (P16), MMP14 and VIM Associated with Giant Cell Tumor of Bone Conceição, André Luis Giacometti [UNESP] Giant cell tumor of bone Gene expression Hypermethylation Immunohistochemistry |
title_short |
Differential Expression of ADAM23, CDKN2A (P16), MMP14 and VIM Associated with Giant Cell Tumor of Bone |
title_full |
Differential Expression of ADAM23, CDKN2A (P16), MMP14 and VIM Associated with Giant Cell Tumor of Bone |
title_fullStr |
Differential Expression of ADAM23, CDKN2A (P16), MMP14 and VIM Associated with Giant Cell Tumor of Bone |
title_full_unstemmed |
Differential Expression of ADAM23, CDKN2A (P16), MMP14 and VIM Associated with Giant Cell Tumor of Bone |
title_sort |
Differential Expression of ADAM23, CDKN2A (P16), MMP14 and VIM Associated with Giant Cell Tumor of Bone |
author |
Conceição, André Luis Giacometti [UNESP] |
author_facet |
Conceição, André Luis Giacometti [UNESP] Babeto, Erica [UNESP] Candido, Natalia Maria [UNESP] Franco, Fernanda Craveiro [UNESP] Zuccari, Débora Aparecida Pires de Campos Bonilha, Jane Lopes Cordeiro, José Antônio Calmon, Marilia Freitas [UNESP] Rahal, Paula [UNESP] |
author_role |
author |
author2 |
Babeto, Erica [UNESP] Candido, Natalia Maria [UNESP] Franco, Fernanda Craveiro [UNESP] Zuccari, Débora Aparecida Pires de Campos Bonilha, Jane Lopes Cordeiro, José Antônio Calmon, Marilia Freitas [UNESP] Rahal, Paula [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Faculdade de Medicina de São José do Rio Preto (FAMERP) |
dc.contributor.author.fl_str_mv |
Conceição, André Luis Giacometti [UNESP] Babeto, Erica [UNESP] Candido, Natalia Maria [UNESP] Franco, Fernanda Craveiro [UNESP] Zuccari, Débora Aparecida Pires de Campos Bonilha, Jane Lopes Cordeiro, José Antônio Calmon, Marilia Freitas [UNESP] Rahal, Paula [UNESP] |
dc.subject.por.fl_str_mv |
Giant cell tumor of bone Gene expression Hypermethylation Immunohistochemistry |
topic |
Giant cell tumor of bone Gene expression Hypermethylation Immunohistochemistry |
description |
Though benign, giant cell tumor of bone (GCTB) can become aggressive and can exhibit a high mitotic rate, necrosis and rarely vascular invasion and metastasis. GCTB has unique histologic characteristics, a high rate of multinucleated cells, a variable and unpredictable growth potential and uncertain biological behavior. In this study, we sought to identify genes differentially expressed in GCTB, thus building a molecular profile of this tumor. We performed quantitative real-time polymerase chain reaction (qPCR), immunohistochemistry and analyses of methylation to identify genes that are putatively associated with GCTB. The expression of the ADAM23 and CDKN2A genes was decreased in GCTB samples compared to normal bone tissue, measured by qPCR. Additionally, a high hypermethylation frequency of the promoter regions of ADAM23 and CDKN2A in GCTB was observed. The expression of the MAP2K3, MMP14, TIMP2 and VIM genes was significantly higher in GCTB than in normal bone tissue, a fact that was confirmed by qPCR and immunohistochemistry. The set of genes identified here furthers our understanding of the molecular basis of GCTB. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-12-07T15:35:15Z 2015-12-07T15:35:15Z 2015 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.7150/jca.11238 Journal Of Cancer, v. 6, n. 7, p. 593-603, 2015. 1837-9664 http://hdl.handle.net/11449/131423 10.7150/jca.11238 PMC4466407.pdf 7991082362671212 26078788 PMC4466407 0000-0001-5693-6148 |
url |
http://dx.doi.org/10.7150/jca.11238 http://hdl.handle.net/11449/131423 |
identifier_str_mv |
Journal Of Cancer, v. 6, n. 7, p. 593-603, 2015. 1837-9664 10.7150/jca.11238 PMC4466407.pdf 7991082362671212 26078788 PMC4466407 0000-0001-5693-6148 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Cancer 3.249 1,159 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
593-603 application/pdf |
dc.publisher.none.fl_str_mv |
Journal Of Cancer |
publisher.none.fl_str_mv |
Journal Of Cancer |
dc.source.none.fl_str_mv |
PubMed reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965005313998848 |