Experimental infection of commercial layers with wild or attenuated Salmonella Gallinarum mutant strains: Anatomic pathology, total blood cell count and serum protein levels
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1516-635X2013000200004 http://hdl.handle.net/11449/76072 |
Resumo: | The aim of the present study was to comparatively evaluate hemogram, blood serum components and anatomopathologic alterations in commercial layers experimentally challenged with an attenuated vaccine candidate strain (SGΔcobSΔcbiA) and other two pathogenic strains (SGΔcobS and SGNalr) of Gallinarum (SG). In total, 280 commercial layers were randomly divided into 4 groups (G1, G2, G3 and G4). At five days of age, birds from groups G1 received approximately 107 colony forming units (CFU) of SGΔcobS; meanwhile birds from group G2 and G3 received the same dose of SGNalr and SGΔcobSΔcbiA, respectively. Birds from G4 were not infected. At 24 hours before (DBI) and 24 hours after (1 DAI), and three (3 DAI), five (5 DAI), seven (7 DAI) ten (10 DAI), and fifteen (15 DAI) days after the infection, 10 birds of each group were humanely killed and blood samples collected to hematological and serum tests. Samples of liver, spleen, thymus, bursa of Fabricius, kidney and heart were also collected for the histological examination. Birds inoculated with SGΔcobS and SGNalr showed similar alterations in hemogram, blood serum components and anatomopathologic exams. On the other hand, the exams of birds inoculated with SGΔcobSΔcbiA strain were similar to those of the uninfected birds. However, changes could be noticed in levels of uric acid and cholesterol during the course of the infection of birds from G3. Decrease in levels of light IgG 3 DAI was also observed in birds from this group. Pyknosis in kidney cells was a microscopic alteration found in birds from G3. Further studies must be done to verify if these alterations will not interfere in the performance of the vaccinate birds with SGΔcobSΔcbiA strain. |
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Experimental infection of commercial layers with wild or attenuated Salmonella Gallinarum mutant strains: Anatomic pathology, total blood cell count and serum protein levelsAcute-phase proteinsAttenuationFowl typhoidHistopathologyLeukogramThe aim of the present study was to comparatively evaluate hemogram, blood serum components and anatomopathologic alterations in commercial layers experimentally challenged with an attenuated vaccine candidate strain (SGΔcobSΔcbiA) and other two pathogenic strains (SGΔcobS and SGNalr) of Gallinarum (SG). In total, 280 commercial layers were randomly divided into 4 groups (G1, G2, G3 and G4). At five days of age, birds from groups G1 received approximately 107 colony forming units (CFU) of SGΔcobS; meanwhile birds from group G2 and G3 received the same dose of SGNalr and SGΔcobSΔcbiA, respectively. Birds from G4 were not infected. At 24 hours before (DBI) and 24 hours after (1 DAI), and three (3 DAI), five (5 DAI), seven (7 DAI) ten (10 DAI), and fifteen (15 DAI) days after the infection, 10 birds of each group were humanely killed and blood samples collected to hematological and serum tests. Samples of liver, spleen, thymus, bursa of Fabricius, kidney and heart were also collected for the histological examination. Birds inoculated with SGΔcobS and SGNalr showed similar alterations in hemogram, blood serum components and anatomopathologic exams. On the other hand, the exams of birds inoculated with SGΔcobSΔcbiA strain were similar to those of the uninfected birds. However, changes could be noticed in levels of uric acid and cholesterol during the course of the infection of birds from G3. Decrease in levels of light IgG 3 DAI was also observed in birds from this group. Pyknosis in kidney cells was a microscopic alteration found in birds from G3. Further studies must be done to verify if these alterations will not interfere in the performance of the vaccinate birds with SGΔcobSΔcbiA strain.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Veterinary Pathology Sao Paulo State University Campus of Jaboticabal, College of Agricultural and Veterinarian Sciences, Via de Acesso Prof.P.D.Castellane, s/n, 14884-900 - Jaboticabal - SPDepartment of Veterinary Clinical Sciences Sao Paulo State University Campus of Jaboticabal, College of Agricultural and Veterinarian Sciences, Via de Acesso Prof.P.D.Castellane, s/n, 14884-900 - Jaboticabal - SPDepartment of Animal Physiology Sao Paulo State University Campus of Jaboticabal, College of Agricultural and Veterinarian Sciences, Via de Acesso Prof.P.D.Castellane,s/n, 14884-900 - Jaboticabal - SPDepartment of Veterinary Pathology Sao Paulo State University Campus of Jaboticabal, College of Agricultural and Veterinarian Sciences, Via de Acesso Prof.P.D.Castellane, s/n, 14884-900 - Jaboticabal - SPDepartment of Veterinary Clinical Sciences Sao Paulo State University Campus of Jaboticabal, College of Agricultural and Veterinarian Sciences, Via de Acesso Prof.P.D.Castellane, s/n, 14884-900 - Jaboticabal - SPDepartment of Animal Physiology Sao Paulo State University Campus of Jaboticabal, College of Agricultural and Veterinarian Sciences, Via de Acesso Prof.P.D.Castellane,s/n, 14884-900 - Jaboticabal - SPUniversidade Estadual Paulista (Unesp)Garcia, K. O. [UNESP]Berchieri, A. [UNESP]Santana, A. M. [UNESP]Alarcon, M. F F [UNESP]Freitas Neto, O. C. [UNESP]Fagliari, J. J. [UNESP]2014-05-27T11:30:03Z2014-05-27T11:30:03Z2013-07-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article91-103application/pdfhttp://dx.doi.org/10.1590/S1516-635X2013000200004Revista Brasileira de Ciencia Avicola, v. 15, n. 2, p. 91-103, 2013.1516-635Xhttp://hdl.handle.net/11449/7607210.1590/S1516-635X2013000200004S1516-635X2013000200004WOS:0003268110000042-s2.0-848805997452-s2.0-84880599745.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira de Ciência Avícola0.463info:eu-repo/semantics/openAccess2024-06-07T13:01:17Zoai:repositorio.unesp.br:11449/76072Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T13:42:33.851280Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Experimental infection of commercial layers with wild or attenuated Salmonella Gallinarum mutant strains: Anatomic pathology, total blood cell count and serum protein levels |
title |
Experimental infection of commercial layers with wild or attenuated Salmonella Gallinarum mutant strains: Anatomic pathology, total blood cell count and serum protein levels |
spellingShingle |
Experimental infection of commercial layers with wild or attenuated Salmonella Gallinarum mutant strains: Anatomic pathology, total blood cell count and serum protein levels Garcia, K. O. [UNESP] Acute-phase proteins Attenuation Fowl typhoid Histopathology Leukogram |
title_short |
Experimental infection of commercial layers with wild or attenuated Salmonella Gallinarum mutant strains: Anatomic pathology, total blood cell count and serum protein levels |
title_full |
Experimental infection of commercial layers with wild or attenuated Salmonella Gallinarum mutant strains: Anatomic pathology, total blood cell count and serum protein levels |
title_fullStr |
Experimental infection of commercial layers with wild or attenuated Salmonella Gallinarum mutant strains: Anatomic pathology, total blood cell count and serum protein levels |
title_full_unstemmed |
Experimental infection of commercial layers with wild or attenuated Salmonella Gallinarum mutant strains: Anatomic pathology, total blood cell count and serum protein levels |
title_sort |
Experimental infection of commercial layers with wild or attenuated Salmonella Gallinarum mutant strains: Anatomic pathology, total blood cell count and serum protein levels |
author |
Garcia, K. O. [UNESP] |
author_facet |
Garcia, K. O. [UNESP] Berchieri, A. [UNESP] Santana, A. M. [UNESP] Alarcon, M. F F [UNESP] Freitas Neto, O. C. [UNESP] Fagliari, J. J. [UNESP] |
author_role |
author |
author2 |
Berchieri, A. [UNESP] Santana, A. M. [UNESP] Alarcon, M. F F [UNESP] Freitas Neto, O. C. [UNESP] Fagliari, J. J. [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Garcia, K. O. [UNESP] Berchieri, A. [UNESP] Santana, A. M. [UNESP] Alarcon, M. F F [UNESP] Freitas Neto, O. C. [UNESP] Fagliari, J. J. [UNESP] |
dc.subject.por.fl_str_mv |
Acute-phase proteins Attenuation Fowl typhoid Histopathology Leukogram |
topic |
Acute-phase proteins Attenuation Fowl typhoid Histopathology Leukogram |
description |
The aim of the present study was to comparatively evaluate hemogram, blood serum components and anatomopathologic alterations in commercial layers experimentally challenged with an attenuated vaccine candidate strain (SGΔcobSΔcbiA) and other two pathogenic strains (SGΔcobS and SGNalr) of Gallinarum (SG). In total, 280 commercial layers were randomly divided into 4 groups (G1, G2, G3 and G4). At five days of age, birds from groups G1 received approximately 107 colony forming units (CFU) of SGΔcobS; meanwhile birds from group G2 and G3 received the same dose of SGNalr and SGΔcobSΔcbiA, respectively. Birds from G4 were not infected. At 24 hours before (DBI) and 24 hours after (1 DAI), and three (3 DAI), five (5 DAI), seven (7 DAI) ten (10 DAI), and fifteen (15 DAI) days after the infection, 10 birds of each group were humanely killed and blood samples collected to hematological and serum tests. Samples of liver, spleen, thymus, bursa of Fabricius, kidney and heart were also collected for the histological examination. Birds inoculated with SGΔcobS and SGNalr showed similar alterations in hemogram, blood serum components and anatomopathologic exams. On the other hand, the exams of birds inoculated with SGΔcobSΔcbiA strain were similar to those of the uninfected birds. However, changes could be noticed in levels of uric acid and cholesterol during the course of the infection of birds from G3. Decrease in levels of light IgG 3 DAI was also observed in birds from this group. Pyknosis in kidney cells was a microscopic alteration found in birds from G3. Further studies must be done to verify if these alterations will not interfere in the performance of the vaccinate birds with SGΔcobSΔcbiA strain. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-07-30 2014-05-27T11:30:03Z 2014-05-27T11:30:03Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1516-635X2013000200004 Revista Brasileira de Ciencia Avicola, v. 15, n. 2, p. 91-103, 2013. 1516-635X http://hdl.handle.net/11449/76072 10.1590/S1516-635X2013000200004 S1516-635X2013000200004 WOS:000326811000004 2-s2.0-84880599745 2-s2.0-84880599745.pdf |
url |
http://dx.doi.org/10.1590/S1516-635X2013000200004 http://hdl.handle.net/11449/76072 |
identifier_str_mv |
Revista Brasileira de Ciencia Avicola, v. 15, n. 2, p. 91-103, 2013. 1516-635X 10.1590/S1516-635X2013000200004 S1516-635X2013000200004 WOS:000326811000004 2-s2.0-84880599745 2-s2.0-84880599745.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista Brasileira de Ciência Avícola 0.463 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
91-103 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128268896829440 |