Collagen Membranes Functionalized with 150 Cycles of Atomic Layer Deposited Titania Improve Osteopromotive Property in Critical-Size Defects Created on Rat Calvaria
Autor(a) principal: | |
---|---|
Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/jfb14030120 http://hdl.handle.net/11449/247065 |
Resumo: | The membranes used in bone reconstructions have been the object of investigation in the field of tissue engineering, seeking to improve their mechanical strength and add other properties, mainly the osteopromotive. This study aimed to evaluate the functionalization of collagen membranes, with atomic layer deposition of TiO2 on the bone repair of critical defects in rat calvaria and subcutaneous biocompatibility. A total of 39 male rats were randomized into four groups: blood clot (BC), collagen membrane (COL), COL 150—150 cycles of titania, and COL 600—600 cycles of titania. The defects were created in each calvaria (5 mm in diameter) and covered according to each group; the animals were euthanized at 7, 14, and 28 days. The collected samples were assessed by histometric (newly bone formed, soft tissue area, membrane area, and residual linear defect) and histologic (inflammatory cells and blood cells count) analysis. All data were subjected to statistical analysis (p < 0.05). The COL150 group showed statistically significant differences compared to the other groups, mainly in the analysis of residual linear defects (1.5 ± 0.5 × 106 pixels/µm2 for COL 150, and around 1 ± 0.5 × 106 pixels/µm2 for the other groups) and newly formed bone (1500 ± 1200 pixels/µm for COL 150, and around 4000 pixels/µm for the others) (p < 0.05), demonstrating a better biological behavior in the chronology of defects repair. It is concluded that the collagen membrane functionalized by TiO2 over 150 cycles showed better bioactive potential in treating critical size defects in the rats’ calvaria. |
id |
UNSP_96964cc43795e476675afb63a02a57f4 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/247065 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Collagen Membranes Functionalized with 150 Cycles of Atomic Layer Deposited Titania Improve Osteopromotive Property in Critical-Size Defects Created on Rat Calvariaatomic layer depositionmembrane functionalizationosseointegrationtitanium dioxideThe membranes used in bone reconstructions have been the object of investigation in the field of tissue engineering, seeking to improve their mechanical strength and add other properties, mainly the osteopromotive. This study aimed to evaluate the functionalization of collagen membranes, with atomic layer deposition of TiO2 on the bone repair of critical defects in rat calvaria and subcutaneous biocompatibility. A total of 39 male rats were randomized into four groups: blood clot (BC), collagen membrane (COL), COL 150—150 cycles of titania, and COL 600—600 cycles of titania. The defects were created in each calvaria (5 mm in diameter) and covered according to each group; the animals were euthanized at 7, 14, and 28 days. The collected samples were assessed by histometric (newly bone formed, soft tissue area, membrane area, and residual linear defect) and histologic (inflammatory cells and blood cells count) analysis. All data were subjected to statistical analysis (p < 0.05). The COL150 group showed statistically significant differences compared to the other groups, mainly in the analysis of residual linear defects (1.5 ± 0.5 × 106 pixels/µm2 for COL 150, and around 1 ± 0.5 × 106 pixels/µm2 for the other groups) and newly formed bone (1500 ± 1200 pixels/µm for COL 150, and around 4000 pixels/µm for the others) (p < 0.05), demonstrating a better biological behavior in the chronology of defects repair. It is concluded that the collagen membrane functionalized by TiO2 over 150 cycles showed better bioactive potential in treating critical size defects in the rats’ calvaria.Center for Hierarchical Manufacturing, National Science FoundationCenter for Selective C-H Functionalization, National Science FoundationNational Coordination OfficeNational Science Foundation, United Arab EmiratesNational Science FoundationNational Science Foundation of Sri LankaOffice of Integrative ActivitiesDepartment of Diagnosis and Surgery School of Dentistry Sao Paulo State University (UNESP)Department of Chemical Engineering University of Illinois ChicagoFaculty of Dental Medicine Universitas AirlanggaBiomedical Engineering Department University of Illinois ChicagoDepartment of Restorative Dentistry University of Illinois ChicagoDepartment of Diagnosis and Surgery School of Dentistry Sao Paulo State University (UNESP)Center for Hierarchical Manufacturing, National Science Foundation: NSF DMR-1309114Center for Selective C-H Functionalization, National Science Foundation: NSF DMR-1309114National Coordination Office: NSF DMR-1309114National Science Foundation, United Arab Emirates: NSF DMR-1309114National Science Foundation: NSF DMR-1309114National Science Foundation of Sri Lanka: NSF DMR-1309114Office of Integrative Activities: NSF DMR-1309114Universidade Estadual Paulista (UNESP)University of Illinois ChicagoUniversitas AirlanggaFaverani, Leonardo P. [UNESP]Astaneh, Sarah HashemiCosta, Monique Gonçalves da [UNESP]Delanora, Leonardo A. [UNESP]Lima-Neto, Tiburtino J. [UNESP]Barbosa, Stéfany [UNESP]Ariani, Maretaningtias DwiTakoudis, ChristosSukotjo, Cortino2023-07-29T12:58:09Z2023-07-29T12:58:09Z2023-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/jfb14030120Journal of Functional Biomaterials, v. 14, n. 3, 2023.2079-4983http://hdl.handle.net/11449/24706510.3390/jfb140301202-s2.0-85151121035Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Functional Biomaterialsinfo:eu-repo/semantics/openAccess2023-07-29T12:58:09Zoai:repositorio.unesp.br:11449/247065Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T12:58:09Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Collagen Membranes Functionalized with 150 Cycles of Atomic Layer Deposited Titania Improve Osteopromotive Property in Critical-Size Defects Created on Rat Calvaria |
title |
Collagen Membranes Functionalized with 150 Cycles of Atomic Layer Deposited Titania Improve Osteopromotive Property in Critical-Size Defects Created on Rat Calvaria |
spellingShingle |
Collagen Membranes Functionalized with 150 Cycles of Atomic Layer Deposited Titania Improve Osteopromotive Property in Critical-Size Defects Created on Rat Calvaria Faverani, Leonardo P. [UNESP] atomic layer deposition membrane functionalization osseointegration titanium dioxide |
title_short |
Collagen Membranes Functionalized with 150 Cycles of Atomic Layer Deposited Titania Improve Osteopromotive Property in Critical-Size Defects Created on Rat Calvaria |
title_full |
Collagen Membranes Functionalized with 150 Cycles of Atomic Layer Deposited Titania Improve Osteopromotive Property in Critical-Size Defects Created on Rat Calvaria |
title_fullStr |
Collagen Membranes Functionalized with 150 Cycles of Atomic Layer Deposited Titania Improve Osteopromotive Property in Critical-Size Defects Created on Rat Calvaria |
title_full_unstemmed |
Collagen Membranes Functionalized with 150 Cycles of Atomic Layer Deposited Titania Improve Osteopromotive Property in Critical-Size Defects Created on Rat Calvaria |
title_sort |
Collagen Membranes Functionalized with 150 Cycles of Atomic Layer Deposited Titania Improve Osteopromotive Property in Critical-Size Defects Created on Rat Calvaria |
author |
Faverani, Leonardo P. [UNESP] |
author_facet |
Faverani, Leonardo P. [UNESP] Astaneh, Sarah Hashemi Costa, Monique Gonçalves da [UNESP] Delanora, Leonardo A. [UNESP] Lima-Neto, Tiburtino J. [UNESP] Barbosa, Stéfany [UNESP] Ariani, Maretaningtias Dwi Takoudis, Christos Sukotjo, Cortino |
author_role |
author |
author2 |
Astaneh, Sarah Hashemi Costa, Monique Gonçalves da [UNESP] Delanora, Leonardo A. [UNESP] Lima-Neto, Tiburtino J. [UNESP] Barbosa, Stéfany [UNESP] Ariani, Maretaningtias Dwi Takoudis, Christos Sukotjo, Cortino |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) University of Illinois Chicago Universitas Airlangga |
dc.contributor.author.fl_str_mv |
Faverani, Leonardo P. [UNESP] Astaneh, Sarah Hashemi Costa, Monique Gonçalves da [UNESP] Delanora, Leonardo A. [UNESP] Lima-Neto, Tiburtino J. [UNESP] Barbosa, Stéfany [UNESP] Ariani, Maretaningtias Dwi Takoudis, Christos Sukotjo, Cortino |
dc.subject.por.fl_str_mv |
atomic layer deposition membrane functionalization osseointegration titanium dioxide |
topic |
atomic layer deposition membrane functionalization osseointegration titanium dioxide |
description |
The membranes used in bone reconstructions have been the object of investigation in the field of tissue engineering, seeking to improve their mechanical strength and add other properties, mainly the osteopromotive. This study aimed to evaluate the functionalization of collagen membranes, with atomic layer deposition of TiO2 on the bone repair of critical defects in rat calvaria and subcutaneous biocompatibility. A total of 39 male rats were randomized into four groups: blood clot (BC), collagen membrane (COL), COL 150—150 cycles of titania, and COL 600—600 cycles of titania. The defects were created in each calvaria (5 mm in diameter) and covered according to each group; the animals were euthanized at 7, 14, and 28 days. The collected samples were assessed by histometric (newly bone formed, soft tissue area, membrane area, and residual linear defect) and histologic (inflammatory cells and blood cells count) analysis. All data were subjected to statistical analysis (p < 0.05). The COL150 group showed statistically significant differences compared to the other groups, mainly in the analysis of residual linear defects (1.5 ± 0.5 × 106 pixels/µm2 for COL 150, and around 1 ± 0.5 × 106 pixels/µm2 for the other groups) and newly formed bone (1500 ± 1200 pixels/µm for COL 150, and around 4000 pixels/µm for the others) (p < 0.05), demonstrating a better biological behavior in the chronology of defects repair. It is concluded that the collagen membrane functionalized by TiO2 over 150 cycles showed better bioactive potential in treating critical size defects in the rats’ calvaria. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T12:58:09Z 2023-07-29T12:58:09Z 2023-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/jfb14030120 Journal of Functional Biomaterials, v. 14, n. 3, 2023. 2079-4983 http://hdl.handle.net/11449/247065 10.3390/jfb14030120 2-s2.0-85151121035 |
url |
http://dx.doi.org/10.3390/jfb14030120 http://hdl.handle.net/11449/247065 |
identifier_str_mv |
Journal of Functional Biomaterials, v. 14, n. 3, 2023. 2079-4983 10.3390/jfb14030120 2-s2.0-85151121035 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Functional Biomaterials |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964961242349568 |