Detecção sorológica e molecular de patógenos transmitidos por carrapatos em cães de uma área endêmica para Leishmania infantum do estado do Mato Grosso do Sul
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/232186 |
Resumo: | Tick-borne pathogens affect a wide range of vertebrate hosts. To identify tick-borne pathogens among dogs from Campo Grande, MS, Brazil testing seropositive for Leishmania infantum (syn. L. chagasi), a serological and molecular study was conducted to detect Ehrlichia canis, Anaplasma platys and Babesia vogeli in 60 serum and spleen samples. A confirmatory diagnosis of L. infantum based on serological and molecular assays was also performed, as was sequence alignment and phylogenetic analysis to assess the identity of the parasite species infecting these animals. IgG antibodies to Ehrlichia spp., B. vogeli and L. infantum were found, respectively, in 39 (65%), 49 (81.6%) and 60 (100%) of the sampled dogs. Twenty-seven (45%), fifty-four (90%), fifty-three (88.3%), two (3.3%) and one (1.6%) dog were positive, respectively, for E. canis, Leishmania spp., Leishmania donovani complex, Babesia sp. and Anaplasma sp. in PCR assays. After sequencing, the amplicons showed 99% of identity with E. canis, B. vogeli, A. platys and Leishmania chagasi isolates. The findings of this study indicate that L. infantum-seropositive dogs from Campo Grande are exposed to multiple tick-borne pathogens, which should therefore be included in the differential diagnosis of dogs with clinical suspicion of leishmaniasis. |
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Detecção sorológica e molecular de patógenos transmitidos por carrapatos em cães de uma área endêmica para Leishmania infantum do estado do Mato Grosso do SulMolecular and serological detection of tick-borne pathogens in dogs from an area endemic for Leishmania infantum in Mato Grosso do Sul, BrazilAnaplasma platysBabesia vogeliCo-infectionDogsEhrlichia canisLeishmania infantumTick-borne pathogens affect a wide range of vertebrate hosts. To identify tick-borne pathogens among dogs from Campo Grande, MS, Brazil testing seropositive for Leishmania infantum (syn. L. chagasi), a serological and molecular study was conducted to detect Ehrlichia canis, Anaplasma platys and Babesia vogeli in 60 serum and spleen samples. A confirmatory diagnosis of L. infantum based on serological and molecular assays was also performed, as was sequence alignment and phylogenetic analysis to assess the identity of the parasite species infecting these animals. IgG antibodies to Ehrlichia spp., B. vogeli and L. infantum were found, respectively, in 39 (65%), 49 (81.6%) and 60 (100%) of the sampled dogs. Twenty-seven (45%), fifty-four (90%), fifty-three (88.3%), two (3.3%) and one (1.6%) dog were positive, respectively, for E. canis, Leishmania spp., Leishmania donovani complex, Babesia sp. and Anaplasma sp. in PCR assays. After sequencing, the amplicons showed 99% of identity with E. canis, B. vogeli, A. platys and Leishmania chagasi isolates. The findings of this study indicate that L. infantum-seropositive dogs from Campo Grande are exposed to multiple tick-borne pathogens, which should therefore be included in the differential diagnosis of dogs with clinical suspicion of leishmaniasis.Universidade Estadual Paulista-Unesp, Jaboticabal, SPUniversidade Católica Dom Bosco-UCDB, Campo Grande, MSUniversidade Estadual Paulista-Unesp, Jaboticabal, SPUniversidade Estadual Paulista (UNESP)Universidade Católica Dom Bosco-UCDBde Sousa, Keyla Carstens Marques [UNESP]André, Marcos Rogério [UNESP]Herrera, Heitor Miragliade Andrade, Gisele BrazilianoJusi, Marcia Mariza Gomes [UNESP]Ladislau, LucianaBarreto, Wanessa Teixeira GomesMachado, Rosangela Zacarias [UNESP]de Oliveira, Gilson Pereira [UNESP]2022-04-29T09:12:16Z2022-04-29T09:12:16Z2013-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article525-531Revista Brasileira de Parasitologia Veterinaria, v. 22, n. 4, p. 525-531, 2013.0103-846Xhttp://hdl.handle.net/11449/2321862-s2.0-84890510318Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengporRevista Brasileira de Parasitologia Veterinariainfo:eu-repo/semantics/openAccess2024-06-07T13:01:27Zoai:repositorio.unesp.br:11449/232186Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:24:46.355468Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Detecção sorológica e molecular de patógenos transmitidos por carrapatos em cães de uma área endêmica para Leishmania infantum do estado do Mato Grosso do Sul Molecular and serological detection of tick-borne pathogens in dogs from an area endemic for Leishmania infantum in Mato Grosso do Sul, Brazil |
title |
Detecção sorológica e molecular de patógenos transmitidos por carrapatos em cães de uma área endêmica para Leishmania infantum do estado do Mato Grosso do Sul |
spellingShingle |
Detecção sorológica e molecular de patógenos transmitidos por carrapatos em cães de uma área endêmica para Leishmania infantum do estado do Mato Grosso do Sul de Sousa, Keyla Carstens Marques [UNESP] Anaplasma platys Babesia vogeli Co-infection Dogs Ehrlichia canis Leishmania infantum |
title_short |
Detecção sorológica e molecular de patógenos transmitidos por carrapatos em cães de uma área endêmica para Leishmania infantum do estado do Mato Grosso do Sul |
title_full |
Detecção sorológica e molecular de patógenos transmitidos por carrapatos em cães de uma área endêmica para Leishmania infantum do estado do Mato Grosso do Sul |
title_fullStr |
Detecção sorológica e molecular de patógenos transmitidos por carrapatos em cães de uma área endêmica para Leishmania infantum do estado do Mato Grosso do Sul |
title_full_unstemmed |
Detecção sorológica e molecular de patógenos transmitidos por carrapatos em cães de uma área endêmica para Leishmania infantum do estado do Mato Grosso do Sul |
title_sort |
Detecção sorológica e molecular de patógenos transmitidos por carrapatos em cães de uma área endêmica para Leishmania infantum do estado do Mato Grosso do Sul |
author |
de Sousa, Keyla Carstens Marques [UNESP] |
author_facet |
de Sousa, Keyla Carstens Marques [UNESP] André, Marcos Rogério [UNESP] Herrera, Heitor Miraglia de Andrade, Gisele Braziliano Jusi, Marcia Mariza Gomes [UNESP] Ladislau, Luciana Barreto, Wanessa Teixeira Gomes Machado, Rosangela Zacarias [UNESP] de Oliveira, Gilson Pereira [UNESP] |
author_role |
author |
author2 |
André, Marcos Rogério [UNESP] Herrera, Heitor Miraglia de Andrade, Gisele Braziliano Jusi, Marcia Mariza Gomes [UNESP] Ladislau, Luciana Barreto, Wanessa Teixeira Gomes Machado, Rosangela Zacarias [UNESP] de Oliveira, Gilson Pereira [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade Católica Dom Bosco-UCDB |
dc.contributor.author.fl_str_mv |
de Sousa, Keyla Carstens Marques [UNESP] André, Marcos Rogério [UNESP] Herrera, Heitor Miraglia de Andrade, Gisele Braziliano Jusi, Marcia Mariza Gomes [UNESP] Ladislau, Luciana Barreto, Wanessa Teixeira Gomes Machado, Rosangela Zacarias [UNESP] de Oliveira, Gilson Pereira [UNESP] |
dc.subject.por.fl_str_mv |
Anaplasma platys Babesia vogeli Co-infection Dogs Ehrlichia canis Leishmania infantum |
topic |
Anaplasma platys Babesia vogeli Co-infection Dogs Ehrlichia canis Leishmania infantum |
description |
Tick-borne pathogens affect a wide range of vertebrate hosts. To identify tick-borne pathogens among dogs from Campo Grande, MS, Brazil testing seropositive for Leishmania infantum (syn. L. chagasi), a serological and molecular study was conducted to detect Ehrlichia canis, Anaplasma platys and Babesia vogeli in 60 serum and spleen samples. A confirmatory diagnosis of L. infantum based on serological and molecular assays was also performed, as was sequence alignment and phylogenetic analysis to assess the identity of the parasite species infecting these animals. IgG antibodies to Ehrlichia spp., B. vogeli and L. infantum were found, respectively, in 39 (65%), 49 (81.6%) and 60 (100%) of the sampled dogs. Twenty-seven (45%), fifty-four (90%), fifty-three (88.3%), two (3.3%) and one (1.6%) dog were positive, respectively, for E. canis, Leishmania spp., Leishmania donovani complex, Babesia sp. and Anaplasma sp. in PCR assays. After sequencing, the amplicons showed 99% of identity with E. canis, B. vogeli, A. platys and Leishmania chagasi isolates. The findings of this study indicate that L. infantum-seropositive dogs from Campo Grande are exposed to multiple tick-borne pathogens, which should therefore be included in the differential diagnosis of dogs with clinical suspicion of leishmaniasis. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-10-01 2022-04-29T09:12:16Z 2022-04-29T09:12:16Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Revista Brasileira de Parasitologia Veterinaria, v. 22, n. 4, p. 525-531, 2013. 0103-846X http://hdl.handle.net/11449/232186 2-s2.0-84890510318 |
identifier_str_mv |
Revista Brasileira de Parasitologia Veterinaria, v. 22, n. 4, p. 525-531, 2013. 0103-846X 2-s2.0-84890510318 |
url |
http://hdl.handle.net/11449/232186 |
dc.language.iso.fl_str_mv |
eng por |
language |
eng por |
dc.relation.none.fl_str_mv |
Revista Brasileira de Parasitologia Veterinaria |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
525-531 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128357160714240 |