Detecção sorológica e molecular de patógenos transmitidos por carrapatos em cães de uma área endêmica para Leishmania infantum do estado do Mato Grosso do Sul

Detalhes bibliográficos
Autor(a) principal: de Sousa, Keyla Carstens Marques [UNESP]
Data de Publicação: 2013
Outros Autores: André, Marcos Rogério [UNESP], Herrera, Heitor Miraglia, de Andrade, Gisele Braziliano, Jusi, Marcia Mariza Gomes [UNESP], Ladislau, Luciana, Barreto, Wanessa Teixeira Gomes, Machado, Rosangela Zacarias [UNESP], de Oliveira, Gilson Pereira [UNESP]
Tipo de documento: Artigo
Idioma: eng
por
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/232186
Resumo: Tick-borne pathogens affect a wide range of vertebrate hosts. To identify tick-borne pathogens among dogs from Campo Grande, MS, Brazil testing seropositive for Leishmania infantum (syn. L. chagasi), a serological and molecular study was conducted to detect Ehrlichia canis, Anaplasma platys and Babesia vogeli in 60 serum and spleen samples. A confirmatory diagnosis of L. infantum based on serological and molecular assays was also performed, as was sequence alignment and phylogenetic analysis to assess the identity of the parasite species infecting these animals. IgG antibodies to Ehrlichia spp., B. vogeli and L. infantum were found, respectively, in 39 (65%), 49 (81.6%) and 60 (100%) of the sampled dogs. Twenty-seven (45%), fifty-four (90%), fifty-three (88.3%), two (3.3%) and one (1.6%) dog were positive, respectively, for E. canis, Leishmania spp., Leishmania donovani complex, Babesia sp. and Anaplasma sp. in PCR assays. After sequencing, the amplicons showed 99% of identity with E. canis, B. vogeli, A. platys and Leishmania chagasi isolates. The findings of this study indicate that L. infantum-seropositive dogs from Campo Grande are exposed to multiple tick-borne pathogens, which should therefore be included in the differential diagnosis of dogs with clinical suspicion of leishmaniasis.
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spelling Detecção sorológica e molecular de patógenos transmitidos por carrapatos em cães de uma área endêmica para Leishmania infantum do estado do Mato Grosso do SulMolecular and serological detection of tick-borne pathogens in dogs from an area endemic for Leishmania infantum in Mato Grosso do Sul, BrazilAnaplasma platysBabesia vogeliCo-infectionDogsEhrlichia canisLeishmania infantumTick-borne pathogens affect a wide range of vertebrate hosts. To identify tick-borne pathogens among dogs from Campo Grande, MS, Brazil testing seropositive for Leishmania infantum (syn. L. chagasi), a serological and molecular study was conducted to detect Ehrlichia canis, Anaplasma platys and Babesia vogeli in 60 serum and spleen samples. A confirmatory diagnosis of L. infantum based on serological and molecular assays was also performed, as was sequence alignment and phylogenetic analysis to assess the identity of the parasite species infecting these animals. IgG antibodies to Ehrlichia spp., B. vogeli and L. infantum were found, respectively, in 39 (65%), 49 (81.6%) and 60 (100%) of the sampled dogs. Twenty-seven (45%), fifty-four (90%), fifty-three (88.3%), two (3.3%) and one (1.6%) dog were positive, respectively, for E. canis, Leishmania spp., Leishmania donovani complex, Babesia sp. and Anaplasma sp. in PCR assays. After sequencing, the amplicons showed 99% of identity with E. canis, B. vogeli, A. platys and Leishmania chagasi isolates. The findings of this study indicate that L. infantum-seropositive dogs from Campo Grande are exposed to multiple tick-borne pathogens, which should therefore be included in the differential diagnosis of dogs with clinical suspicion of leishmaniasis.Universidade Estadual Paulista-Unesp, Jaboticabal, SPUniversidade Católica Dom Bosco-UCDB, Campo Grande, MSUniversidade Estadual Paulista-Unesp, Jaboticabal, SPUniversidade Estadual Paulista (UNESP)Universidade Católica Dom Bosco-UCDBde Sousa, Keyla Carstens Marques [UNESP]André, Marcos Rogério [UNESP]Herrera, Heitor Miragliade Andrade, Gisele BrazilianoJusi, Marcia Mariza Gomes [UNESP]Ladislau, LucianaBarreto, Wanessa Teixeira GomesMachado, Rosangela Zacarias [UNESP]de Oliveira, Gilson Pereira [UNESP]2022-04-29T09:12:16Z2022-04-29T09:12:16Z2013-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article525-531Revista Brasileira de Parasitologia Veterinaria, v. 22, n. 4, p. 525-531, 2013.0103-846Xhttp://hdl.handle.net/11449/2321862-s2.0-84890510318Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengporRevista Brasileira de Parasitologia Veterinariainfo:eu-repo/semantics/openAccess2024-06-07T13:01:27Zoai:repositorio.unesp.br:11449/232186Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:24:46.355468Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Detecção sorológica e molecular de patógenos transmitidos por carrapatos em cães de uma área endêmica para Leishmania infantum do estado do Mato Grosso do Sul
Molecular and serological detection of tick-borne pathogens in dogs from an area endemic for Leishmania infantum in Mato Grosso do Sul, Brazil
title Detecção sorológica e molecular de patógenos transmitidos por carrapatos em cães de uma área endêmica para Leishmania infantum do estado do Mato Grosso do Sul
spellingShingle Detecção sorológica e molecular de patógenos transmitidos por carrapatos em cães de uma área endêmica para Leishmania infantum do estado do Mato Grosso do Sul
de Sousa, Keyla Carstens Marques [UNESP]
Anaplasma platys
Babesia vogeli
Co-infection
Dogs
Ehrlichia canis
Leishmania infantum
title_short Detecção sorológica e molecular de patógenos transmitidos por carrapatos em cães de uma área endêmica para Leishmania infantum do estado do Mato Grosso do Sul
title_full Detecção sorológica e molecular de patógenos transmitidos por carrapatos em cães de uma área endêmica para Leishmania infantum do estado do Mato Grosso do Sul
title_fullStr Detecção sorológica e molecular de patógenos transmitidos por carrapatos em cães de uma área endêmica para Leishmania infantum do estado do Mato Grosso do Sul
title_full_unstemmed Detecção sorológica e molecular de patógenos transmitidos por carrapatos em cães de uma área endêmica para Leishmania infantum do estado do Mato Grosso do Sul
title_sort Detecção sorológica e molecular de patógenos transmitidos por carrapatos em cães de uma área endêmica para Leishmania infantum do estado do Mato Grosso do Sul
author de Sousa, Keyla Carstens Marques [UNESP]
author_facet de Sousa, Keyla Carstens Marques [UNESP]
André, Marcos Rogério [UNESP]
Herrera, Heitor Miraglia
de Andrade, Gisele Braziliano
Jusi, Marcia Mariza Gomes [UNESP]
Ladislau, Luciana
Barreto, Wanessa Teixeira Gomes
Machado, Rosangela Zacarias [UNESP]
de Oliveira, Gilson Pereira [UNESP]
author_role author
author2 André, Marcos Rogério [UNESP]
Herrera, Heitor Miraglia
de Andrade, Gisele Braziliano
Jusi, Marcia Mariza Gomes [UNESP]
Ladislau, Luciana
Barreto, Wanessa Teixeira Gomes
Machado, Rosangela Zacarias [UNESP]
de Oliveira, Gilson Pereira [UNESP]
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Universidade Católica Dom Bosco-UCDB
dc.contributor.author.fl_str_mv de Sousa, Keyla Carstens Marques [UNESP]
André, Marcos Rogério [UNESP]
Herrera, Heitor Miraglia
de Andrade, Gisele Braziliano
Jusi, Marcia Mariza Gomes [UNESP]
Ladislau, Luciana
Barreto, Wanessa Teixeira Gomes
Machado, Rosangela Zacarias [UNESP]
de Oliveira, Gilson Pereira [UNESP]
dc.subject.por.fl_str_mv Anaplasma platys
Babesia vogeli
Co-infection
Dogs
Ehrlichia canis
Leishmania infantum
topic Anaplasma platys
Babesia vogeli
Co-infection
Dogs
Ehrlichia canis
Leishmania infantum
description Tick-borne pathogens affect a wide range of vertebrate hosts. To identify tick-borne pathogens among dogs from Campo Grande, MS, Brazil testing seropositive for Leishmania infantum (syn. L. chagasi), a serological and molecular study was conducted to detect Ehrlichia canis, Anaplasma platys and Babesia vogeli in 60 serum and spleen samples. A confirmatory diagnosis of L. infantum based on serological and molecular assays was also performed, as was sequence alignment and phylogenetic analysis to assess the identity of the parasite species infecting these animals. IgG antibodies to Ehrlichia spp., B. vogeli and L. infantum were found, respectively, in 39 (65%), 49 (81.6%) and 60 (100%) of the sampled dogs. Twenty-seven (45%), fifty-four (90%), fifty-three (88.3%), two (3.3%) and one (1.6%) dog were positive, respectively, for E. canis, Leishmania spp., Leishmania donovani complex, Babesia sp. and Anaplasma sp. in PCR assays. After sequencing, the amplicons showed 99% of identity with E. canis, B. vogeli, A. platys and Leishmania chagasi isolates. The findings of this study indicate that L. infantum-seropositive dogs from Campo Grande are exposed to multiple tick-borne pathogens, which should therefore be included in the differential diagnosis of dogs with clinical suspicion of leishmaniasis.
publishDate 2013
dc.date.none.fl_str_mv 2013-10-01
2022-04-29T09:12:16Z
2022-04-29T09:12:16Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Revista Brasileira de Parasitologia Veterinaria, v. 22, n. 4, p. 525-531, 2013.
0103-846X
http://hdl.handle.net/11449/232186
2-s2.0-84890510318
identifier_str_mv Revista Brasileira de Parasitologia Veterinaria, v. 22, n. 4, p. 525-531, 2013.
0103-846X
2-s2.0-84890510318
url http://hdl.handle.net/11449/232186
dc.language.iso.fl_str_mv eng
por
language eng
por
dc.relation.none.fl_str_mv Revista Brasileira de Parasitologia Veterinaria
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 525-531
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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