Live and Heat-Killed Lactobacillus rhamnosus ATCC 7469 May Induce Modulatory Cytokines Profiles on Macrophages RAW 264.7

Detalhes bibliográficos
Autor(a) principal: Jorjão, Adeline Lacerda [UNESP]
Data de Publicação: 2015
Outros Autores: De Oliveira, Felipe Eduardo [UNESP], Leão, Mariella Vieira Pereira, Carvalho, Cláudio Antonio Talge [UNESP], Jorge, Antonio Olavo Cardoso [UNESP], De Oliveira, Luciane Dias [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1155/2015/716749
http://hdl.handle.net/11449/172255
Resumo: This study aimed to evaluate the capacity of Lactobacillus rhamnosus and/or its products to induce the synthesis of cytokines (TNF-α, IL-1β, IL-4, IL-6, IL-10, and IL-12) by mouse macrophages (RAW 264.7). Three microorganism preparations were used: live L. rhamnosus (LLR) suspension, heat-killed L. rhamnosus (HKLR) suspension, and the supernatant of a heat-killed L. rhamnosus (SHKLR) suspension, which were cultured with macrophages (37°C, 5% CO2) for 2 h and 30 min. After that, cells were cultured for 16 h. The supernatants were used for the quantitation of cytokines, by ELISA. The results were compared with the synthesis induced by lipopolysaccharide (LPS) and analysed, using ANOVA and Tukey test, 5%. LLR and HKLR groups were able to significantly increase the production of TNF-α, IL-6, and IL-10 (P < 0. 05). SHKLR also significantly increased the production of TNF-α and IL-10 (P < 0. 05) but not IL-6 (P > 0. 05). All the L. rhamnosus suspensions were not able to produce detectable levels of IL-1β or significant levels of IL-4 and IL-12 (P > 0. 05). In conclusion, live and heat-killed L. rhamnosus suspensions were able to induce the synthesis of different cytokines with proinflammatory (TNF-α and IL-6) or regulatory (IL-10) functions, suggesting the role of strain L. rhamnosus ATCC 7469 in the modulation or in the stimulation of immune responses.
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spelling Live and Heat-Killed Lactobacillus rhamnosus ATCC 7469 May Induce Modulatory Cytokines Profiles on Macrophages RAW 264.7This study aimed to evaluate the capacity of Lactobacillus rhamnosus and/or its products to induce the synthesis of cytokines (TNF-α, IL-1β, IL-4, IL-6, IL-10, and IL-12) by mouse macrophages (RAW 264.7). Three microorganism preparations were used: live L. rhamnosus (LLR) suspension, heat-killed L. rhamnosus (HKLR) suspension, and the supernatant of a heat-killed L. rhamnosus (SHKLR) suspension, which were cultured with macrophages (37°C, 5% CO2) for 2 h and 30 min. After that, cells were cultured for 16 h. The supernatants were used for the quantitation of cytokines, by ELISA. The results were compared with the synthesis induced by lipopolysaccharide (LPS) and analysed, using ANOVA and Tukey test, 5%. LLR and HKLR groups were able to significantly increase the production of TNF-α, IL-6, and IL-10 (P < 0. 05). SHKLR also significantly increased the production of TNF-α and IL-10 (P < 0. 05) but not IL-6 (P > 0. 05). All the L. rhamnosus suspensions were not able to produce detectable levels of IL-1β or significant levels of IL-4 and IL-12 (P > 0. 05). In conclusion, live and heat-killed L. rhamnosus suspensions were able to induce the synthesis of different cytokines with proinflammatory (TNF-α and IL-6) or regulatory (IL-10) functions, suggesting the role of strain L. rhamnosus ATCC 7469 in the modulation or in the stimulation of immune responses.Department of Biosciences and Oral Diagnosis Laboratory of Microbiology and Immunology Institute of Science and Technology Universidade Estadual Paulista (UNESP), Avenida Engenheiro Francisco José LongoBioscience Basic Institute University of Taubaté, Street Expedicionário Ernesto Pereira 3Department of Restorative Dentistry Institute of Science and Technology Universidade Estadual Paulista (UNESP), Avenida Engenheiro Francisco José LongoDepartment of Biosciences and Oral Diagnosis Laboratory of Microbiology and Immunology Institute of Science and Technology Universidade Estadual Paulista (UNESP), Avenida Engenheiro Francisco José LongoDepartment of Restorative Dentistry Institute of Science and Technology Universidade Estadual Paulista (UNESP), Avenida Engenheiro Francisco José LongoUniversidade Estadual Paulista (Unesp)University of TaubatéJorjão, Adeline Lacerda [UNESP]De Oliveira, Felipe Eduardo [UNESP]Leão, Mariella Vieira PereiraCarvalho, Cláudio Antonio Talge [UNESP]Jorge, Antonio Olavo Cardoso [UNESP]De Oliveira, Luciane Dias [UNESP]2018-12-11T16:59:23Z2018-12-11T16:59:23Z2015-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1155/2015/716749Scientific World Journal, v. 2015.1537-744X2356-6140http://hdl.handle.net/11449/17225510.1155/2015/7167492-s2.0-849488453062-s2.0-84948845306.pdf93046001665831000000-0003-0987-5594Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientific World Journalinfo:eu-repo/semantics/openAccess2023-10-09T06:03:46Zoai:repositorio.unesp.br:11449/172255Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:25:03.238042Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Live and Heat-Killed Lactobacillus rhamnosus ATCC 7469 May Induce Modulatory Cytokines Profiles on Macrophages RAW 264.7
title Live and Heat-Killed Lactobacillus rhamnosus ATCC 7469 May Induce Modulatory Cytokines Profiles on Macrophages RAW 264.7
spellingShingle Live and Heat-Killed Lactobacillus rhamnosus ATCC 7469 May Induce Modulatory Cytokines Profiles on Macrophages RAW 264.7
Jorjão, Adeline Lacerda [UNESP]
title_short Live and Heat-Killed Lactobacillus rhamnosus ATCC 7469 May Induce Modulatory Cytokines Profiles on Macrophages RAW 264.7
title_full Live and Heat-Killed Lactobacillus rhamnosus ATCC 7469 May Induce Modulatory Cytokines Profiles on Macrophages RAW 264.7
title_fullStr Live and Heat-Killed Lactobacillus rhamnosus ATCC 7469 May Induce Modulatory Cytokines Profiles on Macrophages RAW 264.7
title_full_unstemmed Live and Heat-Killed Lactobacillus rhamnosus ATCC 7469 May Induce Modulatory Cytokines Profiles on Macrophages RAW 264.7
title_sort Live and Heat-Killed Lactobacillus rhamnosus ATCC 7469 May Induce Modulatory Cytokines Profiles on Macrophages RAW 264.7
author Jorjão, Adeline Lacerda [UNESP]
author_facet Jorjão, Adeline Lacerda [UNESP]
De Oliveira, Felipe Eduardo [UNESP]
Leão, Mariella Vieira Pereira
Carvalho, Cláudio Antonio Talge [UNESP]
Jorge, Antonio Olavo Cardoso [UNESP]
De Oliveira, Luciane Dias [UNESP]
author_role author
author2 De Oliveira, Felipe Eduardo [UNESP]
Leão, Mariella Vieira Pereira
Carvalho, Cláudio Antonio Talge [UNESP]
Jorge, Antonio Olavo Cardoso [UNESP]
De Oliveira, Luciane Dias [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
University of Taubaté
dc.contributor.author.fl_str_mv Jorjão, Adeline Lacerda [UNESP]
De Oliveira, Felipe Eduardo [UNESP]
Leão, Mariella Vieira Pereira
Carvalho, Cláudio Antonio Talge [UNESP]
Jorge, Antonio Olavo Cardoso [UNESP]
De Oliveira, Luciane Dias [UNESP]
description This study aimed to evaluate the capacity of Lactobacillus rhamnosus and/or its products to induce the synthesis of cytokines (TNF-α, IL-1β, IL-4, IL-6, IL-10, and IL-12) by mouse macrophages (RAW 264.7). Three microorganism preparations were used: live L. rhamnosus (LLR) suspension, heat-killed L. rhamnosus (HKLR) suspension, and the supernatant of a heat-killed L. rhamnosus (SHKLR) suspension, which were cultured with macrophages (37°C, 5% CO2) for 2 h and 30 min. After that, cells were cultured for 16 h. The supernatants were used for the quantitation of cytokines, by ELISA. The results were compared with the synthesis induced by lipopolysaccharide (LPS) and analysed, using ANOVA and Tukey test, 5%. LLR and HKLR groups were able to significantly increase the production of TNF-α, IL-6, and IL-10 (P < 0. 05). SHKLR also significantly increased the production of TNF-α and IL-10 (P < 0. 05) but not IL-6 (P > 0. 05). All the L. rhamnosus suspensions were not able to produce detectable levels of IL-1β or significant levels of IL-4 and IL-12 (P > 0. 05). In conclusion, live and heat-killed L. rhamnosus suspensions were able to induce the synthesis of different cytokines with proinflammatory (TNF-α and IL-6) or regulatory (IL-10) functions, suggesting the role of strain L. rhamnosus ATCC 7469 in the modulation or in the stimulation of immune responses.
publishDate 2015
dc.date.none.fl_str_mv 2015-01-01
2018-12-11T16:59:23Z
2018-12-11T16:59:23Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1155/2015/716749
Scientific World Journal, v. 2015.
1537-744X
2356-6140
http://hdl.handle.net/11449/172255
10.1155/2015/716749
2-s2.0-84948845306
2-s2.0-84948845306.pdf
9304600166583100
0000-0003-0987-5594
url http://dx.doi.org/10.1155/2015/716749
http://hdl.handle.net/11449/172255
identifier_str_mv Scientific World Journal, v. 2015.
1537-744X
2356-6140
10.1155/2015/716749
2-s2.0-84948845306
2-s2.0-84948845306.pdf
9304600166583100
0000-0003-0987-5594
dc.language.iso.fl_str_mv eng
language eng
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.source.none.fl_str_mv Scopus
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instname_str Universidade Estadual Paulista (UNESP)
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