BIOMOLECULES EXTRACTED BY ATPS: PRACTICAL EXAMPLES

Detalhes bibliográficos
Autor(a) principal: Dutra-Molino, J. V.
Data de Publicação: 2014
Outros Autores: Feitosa, V. Araujo, Lencastre-Novaes, L. C. de, Santos-Ebinuma, V. de Carvalho [UNESP], Lopes, A. Moreni, Jozala, A. Faustino, Viana Marques, D. de Araujo, Malpiedi, L. Pellegrini, Pessoa Junior, A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://redalyc.org/articulo.oa?id=62031508001
http://hdl.handle.net/11449/117689
Resumo: The actual biotechnology industry demands fast and economic upstream and downstream processes to purify biomolecules. In this context, different purification techniques, that offer both high recovery and purity to the final product, have been assayed by different research groups. Liquid-liquid extraction with aqueous two-phase systems is one of the most studied methodologies for bio-separation. This technique presents several advantages such as mild conditions of working, cost-effectiveness, short-time consumption and high recovery percentage of the final product. With the aim to present a comparison of liquid-liquid extractions with other techniques, several aqueous two-phase extraction processes of biomolecules are presented in this review. We presented the advantages and disadvantages of them as of the compared systems. In general, the highest final product purities are achieved when different methodologies are combine, being the chromatographic ones the most applied in the last stages for the high purification factor obtained after them. Alternative methodologies, such as aqueous two-phase systems (ATPS), i.e., PEG/salts or ionic liquids; aqueous two-phase micellar systems, using solvents and surfactants; and extractive fermentation with ATPS, are relevant for both cost-effectiveness and time-saving of the purification process.
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spelling BIOMOLECULES EXTRACTED BY ATPS: PRACTICAL EXAMPLESliquid-liquid extractiondownstream processingbiomoleculespolymerssurfactantThe actual biotechnology industry demands fast and economic upstream and downstream processes to purify biomolecules. In this context, different purification techniques, that offer both high recovery and purity to the final product, have been assayed by different research groups. Liquid-liquid extraction with aqueous two-phase systems is one of the most studied methodologies for bio-separation. This technique presents several advantages such as mild conditions of working, cost-effectiveness, short-time consumption and high recovery percentage of the final product. With the aim to present a comparison of liquid-liquid extractions with other techniques, several aqueous two-phase extraction processes of biomolecules are presented in this review. We presented the advantages and disadvantages of them as of the compared systems. In general, the highest final product purities are achieved when different methodologies are combine, being the chromatographic ones the most applied in the last stages for the high purification factor obtained after them. Alternative methodologies, such as aqueous two-phase systems (ATPS), i.e., PEG/salts or ionic liquids; aqueous two-phase micellar systems, using solvents and surfactants; and extractive fermentation with ATPS, are relevant for both cost-effectiveness and time-saving of the purification process.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Sao Paulo, Fac Pharmaceut Sci, Dept Biochem & Pharmaceut Technol, BR-05508 Sao Paulo, BrazilUniv Estadual Paulista, Fac Pharmaceut Sci, Dept Bioproc & Biotechnol, Sao Paulo, BrazilFed Rural Univ Pernambuco UFRPE, Dept Anim Morphol & Physiol, Recife, PE, BrazilUNR, Fac Biochem & Pharmaceut Sci, Dept Phys Chem, Rosario, Santa Fe, ArgentinaUniv Estadual Paulista, Fac Pharmaceut Sci, Dept Bioproc & Biotechnol, Sao Paulo, BrazilFAPESP: 12/12022-6Univ Autonoma Metropolitana-iztapalapaUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Fed Rural Univ Pernambuco UFRPEUNRDutra-Molino, J. V.Feitosa, V. AraujoLencastre-Novaes, L. C. deSantos-Ebinuma, V. de Carvalho [UNESP]Lopes, A. MoreniJozala, A. FaustinoViana Marques, D. de AraujoMalpiedi, L. PellegriniPessoa Junior, A.2015-03-18T15:56:46Z2015-03-18T15:56:46Z2014-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article359-377application/pdfhttp://redalyc.org/articulo.oa?id=62031508001Revista Mexicana De Ingenieria Quimica. Mexico: Univ Autonoma Metropolitana-iztapalapa, v. 13, n. 2, p. 359-377, 2014.1665-2738http://hdl.handle.net/11449/117689WOS:000340227700003WOS000340227700003.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Mexicana De Ingenieria Quimica1.0970,328info:eu-repo/semantics/openAccess2021-10-22T17:57:48Zoai:repositorio.unesp.br:11449/117689Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:14:11.350169Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv BIOMOLECULES EXTRACTED BY ATPS: PRACTICAL EXAMPLES
title BIOMOLECULES EXTRACTED BY ATPS: PRACTICAL EXAMPLES
spellingShingle BIOMOLECULES EXTRACTED BY ATPS: PRACTICAL EXAMPLES
Dutra-Molino, J. V.
liquid-liquid extraction
downstream processing
biomolecules
polymers
surfactant
title_short BIOMOLECULES EXTRACTED BY ATPS: PRACTICAL EXAMPLES
title_full BIOMOLECULES EXTRACTED BY ATPS: PRACTICAL EXAMPLES
title_fullStr BIOMOLECULES EXTRACTED BY ATPS: PRACTICAL EXAMPLES
title_full_unstemmed BIOMOLECULES EXTRACTED BY ATPS: PRACTICAL EXAMPLES
title_sort BIOMOLECULES EXTRACTED BY ATPS: PRACTICAL EXAMPLES
author Dutra-Molino, J. V.
author_facet Dutra-Molino, J. V.
Feitosa, V. Araujo
Lencastre-Novaes, L. C. de
Santos-Ebinuma, V. de Carvalho [UNESP]
Lopes, A. Moreni
Jozala, A. Faustino
Viana Marques, D. de Araujo
Malpiedi, L. Pellegrini
Pessoa Junior, A.
author_role author
author2 Feitosa, V. Araujo
Lencastre-Novaes, L. C. de
Santos-Ebinuma, V. de Carvalho [UNESP]
Lopes, A. Moreni
Jozala, A. Faustino
Viana Marques, D. de Araujo
Malpiedi, L. Pellegrini
Pessoa Junior, A.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Estadual Paulista (Unesp)
Fed Rural Univ Pernambuco UFRPE
UNR
dc.contributor.author.fl_str_mv Dutra-Molino, J. V.
Feitosa, V. Araujo
Lencastre-Novaes, L. C. de
Santos-Ebinuma, V. de Carvalho [UNESP]
Lopes, A. Moreni
Jozala, A. Faustino
Viana Marques, D. de Araujo
Malpiedi, L. Pellegrini
Pessoa Junior, A.
dc.subject.por.fl_str_mv liquid-liquid extraction
downstream processing
biomolecules
polymers
surfactant
topic liquid-liquid extraction
downstream processing
biomolecules
polymers
surfactant
description The actual biotechnology industry demands fast and economic upstream and downstream processes to purify biomolecules. In this context, different purification techniques, that offer both high recovery and purity to the final product, have been assayed by different research groups. Liquid-liquid extraction with aqueous two-phase systems is one of the most studied methodologies for bio-separation. This technique presents several advantages such as mild conditions of working, cost-effectiveness, short-time consumption and high recovery percentage of the final product. With the aim to present a comparison of liquid-liquid extractions with other techniques, several aqueous two-phase extraction processes of biomolecules are presented in this review. We presented the advantages and disadvantages of them as of the compared systems. In general, the highest final product purities are achieved when different methodologies are combine, being the chromatographic ones the most applied in the last stages for the high purification factor obtained after them. Alternative methodologies, such as aqueous two-phase systems (ATPS), i.e., PEG/salts or ionic liquids; aqueous two-phase micellar systems, using solvents and surfactants; and extractive fermentation with ATPS, are relevant for both cost-effectiveness and time-saving of the purification process.
publishDate 2014
dc.date.none.fl_str_mv 2014-08-01
2015-03-18T15:56:46Z
2015-03-18T15:56:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://redalyc.org/articulo.oa?id=62031508001
Revista Mexicana De Ingenieria Quimica. Mexico: Univ Autonoma Metropolitana-iztapalapa, v. 13, n. 2, p. 359-377, 2014.
1665-2738
http://hdl.handle.net/11449/117689
WOS:000340227700003
WOS000340227700003.pdf
url http://redalyc.org/articulo.oa?id=62031508001
http://hdl.handle.net/11449/117689
identifier_str_mv Revista Mexicana De Ingenieria Quimica. Mexico: Univ Autonoma Metropolitana-iztapalapa, v. 13, n. 2, p. 359-377, 2014.
1665-2738
WOS:000340227700003
WOS000340227700003.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista Mexicana De Ingenieria Quimica
1.097
0,328
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 359-377
application/pdf
dc.publisher.none.fl_str_mv Univ Autonoma Metropolitana-iztapalapa
publisher.none.fl_str_mv Univ Autonoma Metropolitana-iztapalapa
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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