BIOMOLECULES EXTRACTED BY ATPS: PRACTICAL EXAMPLES
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://redalyc.org/articulo.oa?id=62031508001 http://hdl.handle.net/11449/117689 |
Resumo: | The actual biotechnology industry demands fast and economic upstream and downstream processes to purify biomolecules. In this context, different purification techniques, that offer both high recovery and purity to the final product, have been assayed by different research groups. Liquid-liquid extraction with aqueous two-phase systems is one of the most studied methodologies for bio-separation. This technique presents several advantages such as mild conditions of working, cost-effectiveness, short-time consumption and high recovery percentage of the final product. With the aim to present a comparison of liquid-liquid extractions with other techniques, several aqueous two-phase extraction processes of biomolecules are presented in this review. We presented the advantages and disadvantages of them as of the compared systems. In general, the highest final product purities are achieved when different methodologies are combine, being the chromatographic ones the most applied in the last stages for the high purification factor obtained after them. Alternative methodologies, such as aqueous two-phase systems (ATPS), i.e., PEG/salts or ionic liquids; aqueous two-phase micellar systems, using solvents and surfactants; and extractive fermentation with ATPS, are relevant for both cost-effectiveness and time-saving of the purification process. |
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BIOMOLECULES EXTRACTED BY ATPS: PRACTICAL EXAMPLESliquid-liquid extractiondownstream processingbiomoleculespolymerssurfactantThe actual biotechnology industry demands fast and economic upstream and downstream processes to purify biomolecules. In this context, different purification techniques, that offer both high recovery and purity to the final product, have been assayed by different research groups. Liquid-liquid extraction with aqueous two-phase systems is one of the most studied methodologies for bio-separation. This technique presents several advantages such as mild conditions of working, cost-effectiveness, short-time consumption and high recovery percentage of the final product. With the aim to present a comparison of liquid-liquid extractions with other techniques, several aqueous two-phase extraction processes of biomolecules are presented in this review. We presented the advantages and disadvantages of them as of the compared systems. In general, the highest final product purities are achieved when different methodologies are combine, being the chromatographic ones the most applied in the last stages for the high purification factor obtained after them. Alternative methodologies, such as aqueous two-phase systems (ATPS), i.e., PEG/salts or ionic liquids; aqueous two-phase micellar systems, using solvents and surfactants; and extractive fermentation with ATPS, are relevant for both cost-effectiveness and time-saving of the purification process.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Sao Paulo, Fac Pharmaceut Sci, Dept Biochem & Pharmaceut Technol, BR-05508 Sao Paulo, BrazilUniv Estadual Paulista, Fac Pharmaceut Sci, Dept Bioproc & Biotechnol, Sao Paulo, BrazilFed Rural Univ Pernambuco UFRPE, Dept Anim Morphol & Physiol, Recife, PE, BrazilUNR, Fac Biochem & Pharmaceut Sci, Dept Phys Chem, Rosario, Santa Fe, ArgentinaUniv Estadual Paulista, Fac Pharmaceut Sci, Dept Bioproc & Biotechnol, Sao Paulo, BrazilFAPESP: 12/12022-6Univ Autonoma Metropolitana-iztapalapaUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Fed Rural Univ Pernambuco UFRPEUNRDutra-Molino, J. V.Feitosa, V. AraujoLencastre-Novaes, L. C. deSantos-Ebinuma, V. de Carvalho [UNESP]Lopes, A. MoreniJozala, A. FaustinoViana Marques, D. de AraujoMalpiedi, L. PellegriniPessoa Junior, A.2015-03-18T15:56:46Z2015-03-18T15:56:46Z2014-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article359-377application/pdfhttp://redalyc.org/articulo.oa?id=62031508001Revista Mexicana De Ingenieria Quimica. Mexico: Univ Autonoma Metropolitana-iztapalapa, v. 13, n. 2, p. 359-377, 2014.1665-2738http://hdl.handle.net/11449/117689WOS:000340227700003WOS000340227700003.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Mexicana De Ingenieria Quimica1.0970,328info:eu-repo/semantics/openAccess2021-10-22T17:57:48Zoai:repositorio.unesp.br:11449/117689Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:14:11.350169Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
BIOMOLECULES EXTRACTED BY ATPS: PRACTICAL EXAMPLES |
title |
BIOMOLECULES EXTRACTED BY ATPS: PRACTICAL EXAMPLES |
spellingShingle |
BIOMOLECULES EXTRACTED BY ATPS: PRACTICAL EXAMPLES Dutra-Molino, J. V. liquid-liquid extraction downstream processing biomolecules polymers surfactant |
title_short |
BIOMOLECULES EXTRACTED BY ATPS: PRACTICAL EXAMPLES |
title_full |
BIOMOLECULES EXTRACTED BY ATPS: PRACTICAL EXAMPLES |
title_fullStr |
BIOMOLECULES EXTRACTED BY ATPS: PRACTICAL EXAMPLES |
title_full_unstemmed |
BIOMOLECULES EXTRACTED BY ATPS: PRACTICAL EXAMPLES |
title_sort |
BIOMOLECULES EXTRACTED BY ATPS: PRACTICAL EXAMPLES |
author |
Dutra-Molino, J. V. |
author_facet |
Dutra-Molino, J. V. Feitosa, V. Araujo Lencastre-Novaes, L. C. de Santos-Ebinuma, V. de Carvalho [UNESP] Lopes, A. Moreni Jozala, A. Faustino Viana Marques, D. de Araujo Malpiedi, L. Pellegrini Pessoa Junior, A. |
author_role |
author |
author2 |
Feitosa, V. Araujo Lencastre-Novaes, L. C. de Santos-Ebinuma, V. de Carvalho [UNESP] Lopes, A. Moreni Jozala, A. Faustino Viana Marques, D. de Araujo Malpiedi, L. Pellegrini Pessoa Junior, A. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) Fed Rural Univ Pernambuco UFRPE UNR |
dc.contributor.author.fl_str_mv |
Dutra-Molino, J. V. Feitosa, V. Araujo Lencastre-Novaes, L. C. de Santos-Ebinuma, V. de Carvalho [UNESP] Lopes, A. Moreni Jozala, A. Faustino Viana Marques, D. de Araujo Malpiedi, L. Pellegrini Pessoa Junior, A. |
dc.subject.por.fl_str_mv |
liquid-liquid extraction downstream processing biomolecules polymers surfactant |
topic |
liquid-liquid extraction downstream processing biomolecules polymers surfactant |
description |
The actual biotechnology industry demands fast and economic upstream and downstream processes to purify biomolecules. In this context, different purification techniques, that offer both high recovery and purity to the final product, have been assayed by different research groups. Liquid-liquid extraction with aqueous two-phase systems is one of the most studied methodologies for bio-separation. This technique presents several advantages such as mild conditions of working, cost-effectiveness, short-time consumption and high recovery percentage of the final product. With the aim to present a comparison of liquid-liquid extractions with other techniques, several aqueous two-phase extraction processes of biomolecules are presented in this review. We presented the advantages and disadvantages of them as of the compared systems. In general, the highest final product purities are achieved when different methodologies are combine, being the chromatographic ones the most applied in the last stages for the high purification factor obtained after them. Alternative methodologies, such as aqueous two-phase systems (ATPS), i.e., PEG/salts or ionic liquids; aqueous two-phase micellar systems, using solvents and surfactants; and extractive fermentation with ATPS, are relevant for both cost-effectiveness and time-saving of the purification process. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-08-01 2015-03-18T15:56:46Z 2015-03-18T15:56:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://redalyc.org/articulo.oa?id=62031508001 Revista Mexicana De Ingenieria Quimica. Mexico: Univ Autonoma Metropolitana-iztapalapa, v. 13, n. 2, p. 359-377, 2014. 1665-2738 http://hdl.handle.net/11449/117689 WOS:000340227700003 WOS000340227700003.pdf |
url |
http://redalyc.org/articulo.oa?id=62031508001 http://hdl.handle.net/11449/117689 |
identifier_str_mv |
Revista Mexicana De Ingenieria Quimica. Mexico: Univ Autonoma Metropolitana-iztapalapa, v. 13, n. 2, p. 359-377, 2014. 1665-2738 WOS:000340227700003 WOS000340227700003.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista Mexicana De Ingenieria Quimica 1.097 0,328 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
359-377 application/pdf |
dc.publisher.none.fl_str_mv |
Univ Autonoma Metropolitana-iztapalapa |
publisher.none.fl_str_mv |
Univ Autonoma Metropolitana-iztapalapa |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129038426832896 |