Evaluation of the aesthetic effect, enamel microhardness and trans-amelodentinal cytotoxicity of a new bleaching agent for professional use containing trimetaphosphate and fluoride

• Autor(a) principal: Felipe Akabane, Sara Tiemi [UNESP]
• Data de Publicação: 2021
• Outros Autores: Danelon, Marcelle [UNESP], Nunes, Gabriel Pereira [UNESP], Gruba, Amanda Scarpin [UNESP], Alberto de Souza-Costa, Carlos [UNESP], Caroline de Oliveira Duque, Carla [UNESP], de Oliveira Gallinari, Marjorie [UNESP], Fraga Briso, André Luiz [UNESP], Botazzo Delbem, Alberto Carlos [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1016/j.jmbbm.2020.104225; http://hdl.handle.net/11449/205594
• topic: Cytotoxicity; Demineralization; Dental bleaching; Dental enamel; Fluoride; Phosphate

This study evaluated the effects of calcium gluconate (CaGlu), sodium fluoride (NaF), sodium trimetaphosphate (TMP), and NaF/TMP when added to a 35% hydrogen peroxide (H2O2) bleaching gel on the color change, enamel hardness, penetration and cytotoxicity trans-amelodentinal. Bovine enamel/dentin disks (n = 288) were divided according to the bleaching gel: 35% H2O2; 35% H2O2 + 0.05% NaF; 35% H2O2 + 0.25% TMP; 35% H2O2 + 0.05% NaF + 0.25% TMP; 35% H2O2 + 0.1% NaF + 1% TMP and 35% H2O2 + 2% CaGlu. The bleaching gels were applied thrice (40 min/session) at 7-day intervals between each application. Then, the color change, percentage of surface hardness loss (%SH), cross-sectional hardness (ΔKHN), trans-amelodentinal penetration of H2O2, cell viability and morphology (MDPC-23 odontoblast-like cells), alkaline phosphatase activity (ALP) and deposition of mineralization nodules were determined. The data were submitted to ANOVA, followed by the Student-Newman-Keuls test (p < 0.05). All bleaching gels showed significant color changes after treatment (p < 0.001). Mineral loss (%SH and ΔKHN) and H2O2 penetration were lower for 35% H2O2/0.1% NaF/1% TMP; 35% H2O2/2% CaGlu, meanwhile, showed higher values, compared to the other groups (p < 0.001). Cell viability was around 9%, except for the bleaching gel containing 35% H2O2/0.1% NaF/1% TMP with 12.8% (p < 0.05). ALP was higher for groups containing TMP compared to other whitening gels (p < 0.05). The formation of mineralization nodules was greater for gels containing NaF/TMP or CaGlu (p < 0.05). The alterations of cell morphology were intense for all bleaching gels. It was concluded that the addition of NaF/TMP in-office bleaching did not interfere in bleaching efficacy, reduced enamel demineralization, H2O2 penetration and cytotoxicity.