Exometaboloma do tegumento da vespa social Polybia paulista por análises GC-MS: bases moleculares do reconhecimento de tipos parentais

• Autor(a) principal: Esteves, Franciele Grego [UNESP]
• Data de Publicação: 2014
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/131727; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2015-10-16/000852183.pdf
• topic: Vespa; Cromatografia a gás; Espectrometria de massa; Tegumento comum

The social wasps have an efficient system of chemical communication by pheromones. Pheromones in social wasps are responsible for inducing activities such as alarm, recruitment, defense colony, beyond recognition of members of the colony itself. These components (mostly are non-polar and volatile) are generally secreted by various exocrine glands, such as venom gland, Dufour gland, Richard gland, Van der Vecht gland, among others. The integument of social wasps is covered by a mixture of waxes and hydrocarbons epicuticular, which together with a variety of other molecules, can play different roles in the chemical communication of these insects, one of those roles and focus of this work is the recognition of individuals intraspecific, more specifically the recognition of parental types (individuals of the same colony). This study aimed to identify the chemical compounds of low molecular masses present in the integument of the social wasp Polybia paulista of three distinct colonies, using gas chromatography and mass spectrometry (GC-MS) in order to characterize the chemical profile that constitutes the chemical cocktail responsible for recognition of the species, as well as the compounds that allow the identification of parental types. A total of 40 compounds were found which are mostly saturated and unsaturated alkanes, and other molecules such as acids, esters, ketone and amide. Among these, 32 compounds are common to the three colonies and apparently are the chemical basis for the recognition of the specie, and the same compounds showed quantitative differences among the three colonies. Specific compounds were also found for each colony, which apparently constitute the chemical basis of recognition of parental types. Thus, the analytical system using gas chromatography-mass spectrometry was efficient to analyze, characterize, identify, and distinguish colonies of the same species by the chemical composition of low molecular weight...