Sex-specific effects of Eugenia punicifolia extract on gastric ulcer healing in rats

Detalhes bibliográficos
Autor(a) principal: Périco, Larissa Lucena [UNESP]
Data de Publicação: 2018
Outros Autores: Rodrigues, Vinícius Peixoto [UNESP], Ohara, Rie [UNESP], Bueno, Gabriela [UNESP], Nunes, Vânia Vasti Alfieri [UNESP], Dos Santos, Raquel Cássia, Camargo, Ana Carolina Lima [UNESP], Justulin, Luis Antônio [UNESP], De Andrade, Sérgio Faloni, Steimbach, Viviane Miranda Bispo, Da Silva, Luísa Mota, Da Rocha, Lúcia Regina Machado [UNESP], Vilegas, Wagner [UNESP], Dos Santos, Catarina [UNESP], Hiruma-Lima, Clélia Akiko [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3748/wjg.v24.i38.4369
http://hdl.handle.net/11449/188227
Resumo: AIM To evaluate the sex-specific effects of a hydroalcoholic extract from Eugenia punicifolia (HEEP) leaves on gastric ulcer healing. METHODS In this rat study involving males, intact (cycling) females, and ovariectomized females, gastric ulcers were induced using acetic acid. A vehicle, lansoprazole, or HEEP was administered for 14 d after ulcer induction. Body weight was monitored throughout the treatment period. At the end of treatment, the rats were euthanized and the following in vivo and in vitro investigations were performed: macroscopic examination of the lesion area and organ weights, biochemical analysis, zymography, and evaluation of protein expression levels. Additionally, the concentration-dependent effect of HEEP was evaluated in terms of subacute toxicity and cytotoxicity. RESULTS Compared to the vehicle, HEEP demonstrated a great healing capacity by substantially reducing the ulcerative lesion area in males (52.44%), intact females (85.22%), and ovariectomized females (65.47%), confirming that HEEP accelerates the healing of acetic acid-induced gastric lesions and suggesting that this effect is modulated by female sex hormones. The antiulcer effect of HEEP was mediated by prostaglandin E2 only in male rats. Overall, the beneficial effect of HEEP was the highest in intact females. Notably, HEEP promoted the expression of vascular endothelial growth factor (intact vs ovariectomized females) and decreased the expression of Caspase-8 and Bcl-2 (intact female vs male or ovariectomized female). Additionally, HEEP enhanced fibroblast proliferation and migration into a wounded area in vitro, confirming its healing effect. Finally, no sign of subacute toxicity or cytotoxicity of HEEP was observed. CONCLUSION In gastric ulcers, HEEP-induced healing (modulated by female sex hormones; in males, mediated by prostaglandin) involves extracellular matrix remodeling, with gastric mucosa cell proliferation and migration.
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spelling Sex-specific effects of Eugenia punicifolia extract on gastric ulcer healing in ratsAcetic acidEugenia punicifoliaGastric ulcer healingMyrtaceaeSex differenceToxicityAIM To evaluate the sex-specific effects of a hydroalcoholic extract from Eugenia punicifolia (HEEP) leaves on gastric ulcer healing. METHODS In this rat study involving males, intact (cycling) females, and ovariectomized females, gastric ulcers were induced using acetic acid. A vehicle, lansoprazole, or HEEP was administered for 14 d after ulcer induction. Body weight was monitored throughout the treatment period. At the end of treatment, the rats were euthanized and the following in vivo and in vitro investigations were performed: macroscopic examination of the lesion area and organ weights, biochemical analysis, zymography, and evaluation of protein expression levels. Additionally, the concentration-dependent effect of HEEP was evaluated in terms of subacute toxicity and cytotoxicity. RESULTS Compared to the vehicle, HEEP demonstrated a great healing capacity by substantially reducing the ulcerative lesion area in males (52.44%), intact females (85.22%), and ovariectomized females (65.47%), confirming that HEEP accelerates the healing of acetic acid-induced gastric lesions and suggesting that this effect is modulated by female sex hormones. The antiulcer effect of HEEP was mediated by prostaglandin E2 only in male rats. Overall, the beneficial effect of HEEP was the highest in intact females. Notably, HEEP promoted the expression of vascular endothelial growth factor (intact vs ovariectomized females) and decreased the expression of Caspase-8 and Bcl-2 (intact female vs male or ovariectomized female). Additionally, HEEP enhanced fibroblast proliferation and migration into a wounded area in vitro, confirming its healing effect. Finally, no sign of subacute toxicity or cytotoxicity of HEEP was observed. CONCLUSION In gastric ulcers, HEEP-induced healing (modulated by female sex hormones; in males, mediated by prostaglandin) involves extracellular matrix remodeling, with gastric mucosa cell proliferation and migration.Department of Physiology Institute of Biosciences São Paulo State University (UNESP)Laboratory of Bioactive Compounds São Francisco UniversityDepartment of Morphology Institute of Biosciences São Paulo State University (UNESP)Programa de Pós-graduação em Ciências Farmacêuticas Núcleo de Investigações Químico-Farmacêuticas (NIQFAR) Universidade do Vale do Itajaí - UNIVALICoastal Campus of São Vicente São Paulo State University (UNESP), 11330-900Department of Biological Science Faculty of Sciences and Languages São Paulo State University (UNESP)Department of Physiology Institute of Biosciences São Paulo State University (UNESP)Department of Morphology Institute of Biosciences São Paulo State University (UNESP)Coastal Campus of São Vicente São Paulo State University (UNESP), 11330-900Department of Biological Science Faculty of Sciences and Languages São Paulo State University (UNESP)Universidade Estadual Paulista (Unesp)São Francisco UniversityUniversidade do Vale do Itajaí - UNIVALIPérico, Larissa Lucena [UNESP]Rodrigues, Vinícius Peixoto [UNESP]Ohara, Rie [UNESP]Bueno, Gabriela [UNESP]Nunes, Vânia Vasti Alfieri [UNESP]Dos Santos, Raquel CássiaCamargo, Ana Carolina Lima [UNESP]Justulin, Luis Antônio [UNESP]De Andrade, Sérgio FaloniSteimbach, Viviane Miranda BispoDa Silva, Luísa MotaDa Rocha, Lúcia Regina Machado [UNESP]Vilegas, Wagner [UNESP]Dos Santos, Catarina [UNESP]Hiruma-Lima, Clélia Akiko [UNESP]2019-10-06T16:01:22Z2019-10-06T16:01:22Z2018-10-14info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article4369-4383http://dx.doi.org/10.3748/wjg.v24.i38.4369World Journal of Gastroenterology, v. 24, n. 38, p. 4369-4383, 2018.2219-28401007-9327http://hdl.handle.net/11449/18822710.3748/wjg.v24.i38.43692-s2.0-8505507155138145049013868440000-0002-8645-3777Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengWorld Journal of Gastroenterologyinfo:eu-repo/semantics/openAccess2022-02-09T00:56:54Zoai:repositorio.unesp.br:11449/188227Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:34:07.038809Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Sex-specific effects of Eugenia punicifolia extract on gastric ulcer healing in rats
title Sex-specific effects of Eugenia punicifolia extract on gastric ulcer healing in rats
spellingShingle Sex-specific effects of Eugenia punicifolia extract on gastric ulcer healing in rats
Périco, Larissa Lucena [UNESP]
Acetic acid
Eugenia punicifolia
Gastric ulcer healing
Myrtaceae
Sex difference
Toxicity
title_short Sex-specific effects of Eugenia punicifolia extract on gastric ulcer healing in rats
title_full Sex-specific effects of Eugenia punicifolia extract on gastric ulcer healing in rats
title_fullStr Sex-specific effects of Eugenia punicifolia extract on gastric ulcer healing in rats
title_full_unstemmed Sex-specific effects of Eugenia punicifolia extract on gastric ulcer healing in rats
title_sort Sex-specific effects of Eugenia punicifolia extract on gastric ulcer healing in rats
author Périco, Larissa Lucena [UNESP]
author_facet Périco, Larissa Lucena [UNESP]
Rodrigues, Vinícius Peixoto [UNESP]
Ohara, Rie [UNESP]
Bueno, Gabriela [UNESP]
Nunes, Vânia Vasti Alfieri [UNESP]
Dos Santos, Raquel Cássia
Camargo, Ana Carolina Lima [UNESP]
Justulin, Luis Antônio [UNESP]
De Andrade, Sérgio Faloni
Steimbach, Viviane Miranda Bispo
Da Silva, Luísa Mota
Da Rocha, Lúcia Regina Machado [UNESP]
Vilegas, Wagner [UNESP]
Dos Santos, Catarina [UNESP]
Hiruma-Lima, Clélia Akiko [UNESP]
author_role author
author2 Rodrigues, Vinícius Peixoto [UNESP]
Ohara, Rie [UNESP]
Bueno, Gabriela [UNESP]
Nunes, Vânia Vasti Alfieri [UNESP]
Dos Santos, Raquel Cássia
Camargo, Ana Carolina Lima [UNESP]
Justulin, Luis Antônio [UNESP]
De Andrade, Sérgio Faloni
Steimbach, Viviane Miranda Bispo
Da Silva, Luísa Mota
Da Rocha, Lúcia Regina Machado [UNESP]
Vilegas, Wagner [UNESP]
Dos Santos, Catarina [UNESP]
Hiruma-Lima, Clélia Akiko [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
São Francisco University
Universidade do Vale do Itajaí - UNIVALI
dc.contributor.author.fl_str_mv Périco, Larissa Lucena [UNESP]
Rodrigues, Vinícius Peixoto [UNESP]
Ohara, Rie [UNESP]
Bueno, Gabriela [UNESP]
Nunes, Vânia Vasti Alfieri [UNESP]
Dos Santos, Raquel Cássia
Camargo, Ana Carolina Lima [UNESP]
Justulin, Luis Antônio [UNESP]
De Andrade, Sérgio Faloni
Steimbach, Viviane Miranda Bispo
Da Silva, Luísa Mota
Da Rocha, Lúcia Regina Machado [UNESP]
Vilegas, Wagner [UNESP]
Dos Santos, Catarina [UNESP]
Hiruma-Lima, Clélia Akiko [UNESP]
dc.subject.por.fl_str_mv Acetic acid
Eugenia punicifolia
Gastric ulcer healing
Myrtaceae
Sex difference
Toxicity
topic Acetic acid
Eugenia punicifolia
Gastric ulcer healing
Myrtaceae
Sex difference
Toxicity
description AIM To evaluate the sex-specific effects of a hydroalcoholic extract from Eugenia punicifolia (HEEP) leaves on gastric ulcer healing. METHODS In this rat study involving males, intact (cycling) females, and ovariectomized females, gastric ulcers were induced using acetic acid. A vehicle, lansoprazole, or HEEP was administered for 14 d after ulcer induction. Body weight was monitored throughout the treatment period. At the end of treatment, the rats were euthanized and the following in vivo and in vitro investigations were performed: macroscopic examination of the lesion area and organ weights, biochemical analysis, zymography, and evaluation of protein expression levels. Additionally, the concentration-dependent effect of HEEP was evaluated in terms of subacute toxicity and cytotoxicity. RESULTS Compared to the vehicle, HEEP demonstrated a great healing capacity by substantially reducing the ulcerative lesion area in males (52.44%), intact females (85.22%), and ovariectomized females (65.47%), confirming that HEEP accelerates the healing of acetic acid-induced gastric lesions and suggesting that this effect is modulated by female sex hormones. The antiulcer effect of HEEP was mediated by prostaglandin E2 only in male rats. Overall, the beneficial effect of HEEP was the highest in intact females. Notably, HEEP promoted the expression of vascular endothelial growth factor (intact vs ovariectomized females) and decreased the expression of Caspase-8 and Bcl-2 (intact female vs male or ovariectomized female). Additionally, HEEP enhanced fibroblast proliferation and migration into a wounded area in vitro, confirming its healing effect. Finally, no sign of subacute toxicity or cytotoxicity of HEEP was observed. CONCLUSION In gastric ulcers, HEEP-induced healing (modulated by female sex hormones; in males, mediated by prostaglandin) involves extracellular matrix remodeling, with gastric mucosa cell proliferation and migration.
publishDate 2018
dc.date.none.fl_str_mv 2018-10-14
2019-10-06T16:01:22Z
2019-10-06T16:01:22Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3748/wjg.v24.i38.4369
World Journal of Gastroenterology, v. 24, n. 38, p. 4369-4383, 2018.
2219-2840
1007-9327
http://hdl.handle.net/11449/188227
10.3748/wjg.v24.i38.4369
2-s2.0-85055071551
3814504901386844
0000-0002-8645-3777
url http://dx.doi.org/10.3748/wjg.v24.i38.4369
http://hdl.handle.net/11449/188227
identifier_str_mv World Journal of Gastroenterology, v. 24, n. 38, p. 4369-4383, 2018.
2219-2840
1007-9327
10.3748/wjg.v24.i38.4369
2-s2.0-85055071551
3814504901386844
0000-0002-8645-3777
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv World Journal of Gastroenterology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 4369-4383
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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