Influence of the Vehicle and Antibiotic Formulation on Cytotoxicity of Triple Antibiotic Paste
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.joen.2018.09.009 http://hdl.handle.net/11449/188392 |
Resumo: | Introduction: The aim of this study was to assess the influence of antibiotic formulations (tablet/capsule [TC] or United States Pharmacopeia [USP]-grade antibiotics) and vehicles (water [H 2 O] or macrogol + propylene glycol [MP]) on the cytotoxicity and pH of triple antibiotic pastes (TAPs). Methods: L929 fibroblasts were exposed to TAPs prepared with TC or USP-grade antibiotics mixed with H 2 O or MP for 72 hours. Each isolated antibiotic with each vehicle, each isolated vehicle, and the culture medium were used as controls. Cytotoxicity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and neutral red assays. The pH was measured after 3 and 8 hours of immersion of the pastes in water. Data were analyzed using analysis of variance, the Bonferroni or Tukey posttests (α = 0.05), and the Pearson correlation test (α = 0.05). Results: The pastes prepared with TC were less cytotoxic than pastes prepared with USP-grade antibiotics (P <.05), and pastes with the MP vehicle were less cytotoxic than pastes with H 2 O (P <.05). TC TAP + MP showed the lowest cytotoxicity, whereas USP-grade TAP + H 2 O showed the highest cytotoxicity (P <.05). All TAPs showed a pH ranging from 4.64–5.20. Irrespective of the vehicle, USP-grade TAP showed a lower pH than TC TAP (P <.05). TAPs with H 2 O had a lower pH than TAPs with MP (P <.05). Conclusions: The vehicle and the antibiotic formulations influenced the cytotoxicity and pH of TAP. The pastes prepared with TC and MP were less acidic and less cytotoxic than the type prepared with USP-grade antibiotics and H 2 O. |
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Repositório Institucional da UNESP |
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2946 |
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Influence of the Vehicle and Antibiotic Formulation on Cytotoxicity of Triple Antibiotic PasteAntibiotic formulationscytotoxicityregenerative endodonticsroot canal dressingtriple antibiotic pasteIntroduction: The aim of this study was to assess the influence of antibiotic formulations (tablet/capsule [TC] or United States Pharmacopeia [USP]-grade antibiotics) and vehicles (water [H 2 O] or macrogol + propylene glycol [MP]) on the cytotoxicity and pH of triple antibiotic pastes (TAPs). Methods: L929 fibroblasts were exposed to TAPs prepared with TC or USP-grade antibiotics mixed with H 2 O or MP for 72 hours. Each isolated antibiotic with each vehicle, each isolated vehicle, and the culture medium were used as controls. Cytotoxicity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and neutral red assays. The pH was measured after 3 and 8 hours of immersion of the pastes in water. Data were analyzed using analysis of variance, the Bonferroni or Tukey posttests (α = 0.05), and the Pearson correlation test (α = 0.05). Results: The pastes prepared with TC were less cytotoxic than pastes prepared with USP-grade antibiotics (P <.05), and pastes with the MP vehicle were less cytotoxic than pastes with H 2 O (P <.05). TC TAP + MP showed the lowest cytotoxicity, whereas USP-grade TAP + H 2 O showed the highest cytotoxicity (P <.05). All TAPs showed a pH ranging from 4.64–5.20. Irrespective of the vehicle, USP-grade TAP showed a lower pH than TC TAP (P <.05). TAPs with H 2 O had a lower pH than TAPs with MP (P <.05). Conclusions: The vehicle and the antibiotic formulations influenced the cytotoxicity and pH of TAP. The pastes prepared with TC and MP were less acidic and less cytotoxic than the type prepared with USP-grade antibiotics and H 2 O.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Restorative Dentistry Araraquara School of Dentistry São Paulo State University-UNESPDepartment of Pediatric Dentistry Alfenas School of Dentistry Jose Rosario Vellano University-UnifenasDepartment of Restorative Dentistry Araraquara School of Dentistry São Paulo State University-UNESPFAPESP: 2011/19511-0Universidade Estadual Paulista (Unesp)Jose Rosario Vellano University-UnifenasFaria, Gisele [UNESP]Rodrigues, Elisandra Márcia [UNESP]Coaguila-Llerena, Hernán [UNESP]Gomes-Cornélio, Ana Lívia [UNESP]Neto Angéloco, Renan Rodrigues [UNESP]Swerts Pereira, Maristela SoaresTanomaru Filho, Mario [UNESP]2019-10-06T16:06:34Z2019-10-06T16:06:34Z2018-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1812-1816http://dx.doi.org/10.1016/j.joen.2018.09.009Journal of Endodontics, v. 44, n. 12, p. 1812-1816, 2018.0099-2399http://hdl.handle.net/11449/18839210.1016/j.joen.2018.09.0092-s2.0-850569729192665211298584751Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Endodonticsinfo:eu-repo/semantics/openAccess2021-10-23T19:49:54Zoai:repositorio.unesp.br:11449/188392Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:16:01.258030Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Influence of the Vehicle and Antibiotic Formulation on Cytotoxicity of Triple Antibiotic Paste |
title |
Influence of the Vehicle and Antibiotic Formulation on Cytotoxicity of Triple Antibiotic Paste |
spellingShingle |
Influence of the Vehicle and Antibiotic Formulation on Cytotoxicity of Triple Antibiotic Paste Faria, Gisele [UNESP] Antibiotic formulations cytotoxicity regenerative endodontics root canal dressing triple antibiotic paste |
title_short |
Influence of the Vehicle and Antibiotic Formulation on Cytotoxicity of Triple Antibiotic Paste |
title_full |
Influence of the Vehicle and Antibiotic Formulation on Cytotoxicity of Triple Antibiotic Paste |
title_fullStr |
Influence of the Vehicle and Antibiotic Formulation on Cytotoxicity of Triple Antibiotic Paste |
title_full_unstemmed |
Influence of the Vehicle and Antibiotic Formulation on Cytotoxicity of Triple Antibiotic Paste |
title_sort |
Influence of the Vehicle and Antibiotic Formulation on Cytotoxicity of Triple Antibiotic Paste |
author |
Faria, Gisele [UNESP] |
author_facet |
Faria, Gisele [UNESP] Rodrigues, Elisandra Márcia [UNESP] Coaguila-Llerena, Hernán [UNESP] Gomes-Cornélio, Ana Lívia [UNESP] Neto Angéloco, Renan Rodrigues [UNESP] Swerts Pereira, Maristela Soares Tanomaru Filho, Mario [UNESP] |
author_role |
author |
author2 |
Rodrigues, Elisandra Márcia [UNESP] Coaguila-Llerena, Hernán [UNESP] Gomes-Cornélio, Ana Lívia [UNESP] Neto Angéloco, Renan Rodrigues [UNESP] Swerts Pereira, Maristela Soares Tanomaru Filho, Mario [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Jose Rosario Vellano University-Unifenas |
dc.contributor.author.fl_str_mv |
Faria, Gisele [UNESP] Rodrigues, Elisandra Márcia [UNESP] Coaguila-Llerena, Hernán [UNESP] Gomes-Cornélio, Ana Lívia [UNESP] Neto Angéloco, Renan Rodrigues [UNESP] Swerts Pereira, Maristela Soares Tanomaru Filho, Mario [UNESP] |
dc.subject.por.fl_str_mv |
Antibiotic formulations cytotoxicity regenerative endodontics root canal dressing triple antibiotic paste |
topic |
Antibiotic formulations cytotoxicity regenerative endodontics root canal dressing triple antibiotic paste |
description |
Introduction: The aim of this study was to assess the influence of antibiotic formulations (tablet/capsule [TC] or United States Pharmacopeia [USP]-grade antibiotics) and vehicles (water [H 2 O] or macrogol + propylene glycol [MP]) on the cytotoxicity and pH of triple antibiotic pastes (TAPs). Methods: L929 fibroblasts were exposed to TAPs prepared with TC or USP-grade antibiotics mixed with H 2 O or MP for 72 hours. Each isolated antibiotic with each vehicle, each isolated vehicle, and the culture medium were used as controls. Cytotoxicity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and neutral red assays. The pH was measured after 3 and 8 hours of immersion of the pastes in water. Data were analyzed using analysis of variance, the Bonferroni or Tukey posttests (α = 0.05), and the Pearson correlation test (α = 0.05). Results: The pastes prepared with TC were less cytotoxic than pastes prepared with USP-grade antibiotics (P <.05), and pastes with the MP vehicle were less cytotoxic than pastes with H 2 O (P <.05). TC TAP + MP showed the lowest cytotoxicity, whereas USP-grade TAP + H 2 O showed the highest cytotoxicity (P <.05). All TAPs showed a pH ranging from 4.64–5.20. Irrespective of the vehicle, USP-grade TAP showed a lower pH than TC TAP (P <.05). TAPs with H 2 O had a lower pH than TAPs with MP (P <.05). Conclusions: The vehicle and the antibiotic formulations influenced the cytotoxicity and pH of TAP. The pastes prepared with TC and MP were less acidic and less cytotoxic than the type prepared with USP-grade antibiotics and H 2 O. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-01 2019-10-06T16:06:34Z 2019-10-06T16:06:34Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.joen.2018.09.009 Journal of Endodontics, v. 44, n. 12, p. 1812-1816, 2018. 0099-2399 http://hdl.handle.net/11449/188392 10.1016/j.joen.2018.09.009 2-s2.0-85056972919 2665211298584751 |
url |
http://dx.doi.org/10.1016/j.joen.2018.09.009 http://hdl.handle.net/11449/188392 |
identifier_str_mv |
Journal of Endodontics, v. 44, n. 12, p. 1812-1816, 2018. 0099-2399 10.1016/j.joen.2018.09.009 2-s2.0-85056972919 2665211298584751 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Endodontics |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1812-1816 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128781031833600 |