Antimicrobial effects of silver nanoparticles and extracts of Syzygium cumini flowers and seeds: Periodontal, cariogenic and opportunistic pathogens
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.archoralbio.2021.105101 http://hdl.handle.net/11449/210233 |
Resumo: | Objective: This study aimed to analyze the antimicrobial effects of lyophilized hydroalcoholic extract (HEScSeed and HEScFlower) and silver nanoparticles (AgNPs-HEScSeed and AgNPs-HEScFlower) of S. cumini seed and flower, and to characterize some compounds of these extracts and their NPs. Design: Phytochemical screening was performed by GC-MS. Nanoparticles were characterized by UV-vis spectroscopy, energy-dispersive X-ray (EDX) spectrophotometry, scanning electron microscopy (SEM) and field emission gun (FEG), dynamic light scattering (DLS) and zeta potential (ZP). Antimicrobial susceptibility tests were analyzed by broth microdilution and agar diffusion methods. Results: HEScSeed and HEScFlower showed 7 and 17 phytochemical compounds, respectively. AgNPs-plant extracts were reported as stable and with variable shapes and sizes. All studied species (A. naeslundii, C. albicans, F. nucleatum, S. aureus, S. epidermidis, S. mutans, S. oralis and V. dispar) were susceptible to extracts and AgNPs-plant extracts, with varying degrees of antimicrobial activities (extract: 648.4-5,187.5 mu g/mL; AgNPs-plant: 31.2-2,000 mu g/mL). Conclusion: The extracts of S. cumini seed and flower have antimicrobial action against pathogens of medical and dental interest, whose MIC and MMC are species-dependent. The AgNPs-HEScSeed and AgNPs-HEScFlower have different shapes, sizes, organic compounds, stability and electronegativity (capping), characteristics that contribute to their bacteriostatic and fungistatic effects, but at significantly lower concentrations than plant extracts. |
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Antimicrobial effects of silver nanoparticles and extracts of Syzygium cumini flowers and seeds: Periodontal, cariogenic and opportunistic pathogensAntimicrobial bioactivityGC-MSGreen synthesisMedical and oral pathogensPlant extractObjective: This study aimed to analyze the antimicrobial effects of lyophilized hydroalcoholic extract (HEScSeed and HEScFlower) and silver nanoparticles (AgNPs-HEScSeed and AgNPs-HEScFlower) of S. cumini seed and flower, and to characterize some compounds of these extracts and their NPs. Design: Phytochemical screening was performed by GC-MS. Nanoparticles were characterized by UV-vis spectroscopy, energy-dispersive X-ray (EDX) spectrophotometry, scanning electron microscopy (SEM) and field emission gun (FEG), dynamic light scattering (DLS) and zeta potential (ZP). Antimicrobial susceptibility tests were analyzed by broth microdilution and agar diffusion methods. Results: HEScSeed and HEScFlower showed 7 and 17 phytochemical compounds, respectively. AgNPs-plant extracts were reported as stable and with variable shapes and sizes. All studied species (A. naeslundii, C. albicans, F. nucleatum, S. aureus, S. epidermidis, S. mutans, S. oralis and V. dispar) were susceptible to extracts and AgNPs-plant extracts, with varying degrees of antimicrobial activities (extract: 648.4-5,187.5 mu g/mL; AgNPs-plant: 31.2-2,000 mu g/mL). Conclusion: The extracts of S. cumini seed and flower have antimicrobial action against pathogens of medical and dental interest, whose MIC and MMC are species-dependent. The AgNPs-HEScSeed and AgNPs-HEScFlower have different shapes, sizes, organic compounds, stability and electronegativity (capping), characteristics that contribute to their bacteriostatic and fungistatic effects, but at significantly lower concentrations than plant extracts.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Sao Paulo State Univ, Sch Dent, Dept Physiol & Pathol, Araraquara, SP, BrazilUniv Estadual Campinas, Dent Sch Piracicaba, Dept Oral Diag, Piracicaba, SP, BrazilUniv Fed Sao Carlos, Sch Chem, Sao Carlos, SP, BrazilSao Paulo State Univ, Sch Dent, Dept Physiol & Pathol, Araraquara, SP, BrazilElsevier B.V.Universidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Universidade Federal de São Carlos (UFSCar)Carvalho Bernardo, Wagner Luis de [UNESP]Gomes Boriollo, Marcelo FabianoTonon, Caroline Coradi [UNESP]Silva, Jeferson Junior daCruz, Fernando MoraesMartins, Adriano LuisHofling, Jose FranciscoPalomari Spolidorio, Denise Madalena [UNESP]2021-06-25T15:02:13Z2021-06-25T15:02:13Z2021-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article12http://dx.doi.org/10.1016/j.archoralbio.2021.105101Archives Of Oral Biology. Oxford: Pergamon-elsevier Science Ltd, v. 125, 12 p., 2021.0003-9969http://hdl.handle.net/11449/21023310.1016/j.archoralbio.2021.105101WOS:000640056400012Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives Of Oral Biologyinfo:eu-repo/semantics/openAccess2021-10-23T20:11:46Zoai:repositorio.unesp.br:11449/210233Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:22:27.120866Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Antimicrobial effects of silver nanoparticles and extracts of Syzygium cumini flowers and seeds: Periodontal, cariogenic and opportunistic pathogens |
title |
Antimicrobial effects of silver nanoparticles and extracts of Syzygium cumini flowers and seeds: Periodontal, cariogenic and opportunistic pathogens |
spellingShingle |
Antimicrobial effects of silver nanoparticles and extracts of Syzygium cumini flowers and seeds: Periodontal, cariogenic and opportunistic pathogens Carvalho Bernardo, Wagner Luis de [UNESP] Antimicrobial bioactivity GC-MS Green synthesis Medical and oral pathogens Plant extract |
title_short |
Antimicrobial effects of silver nanoparticles and extracts of Syzygium cumini flowers and seeds: Periodontal, cariogenic and opportunistic pathogens |
title_full |
Antimicrobial effects of silver nanoparticles and extracts of Syzygium cumini flowers and seeds: Periodontal, cariogenic and opportunistic pathogens |
title_fullStr |
Antimicrobial effects of silver nanoparticles and extracts of Syzygium cumini flowers and seeds: Periodontal, cariogenic and opportunistic pathogens |
title_full_unstemmed |
Antimicrobial effects of silver nanoparticles and extracts of Syzygium cumini flowers and seeds: Periodontal, cariogenic and opportunistic pathogens |
title_sort |
Antimicrobial effects of silver nanoparticles and extracts of Syzygium cumini flowers and seeds: Periodontal, cariogenic and opportunistic pathogens |
author |
Carvalho Bernardo, Wagner Luis de [UNESP] |
author_facet |
Carvalho Bernardo, Wagner Luis de [UNESP] Gomes Boriollo, Marcelo Fabiano Tonon, Caroline Coradi [UNESP] Silva, Jeferson Junior da Cruz, Fernando Moraes Martins, Adriano Luis Hofling, Jose Francisco Palomari Spolidorio, Denise Madalena [UNESP] |
author_role |
author |
author2 |
Gomes Boriollo, Marcelo Fabiano Tonon, Caroline Coradi [UNESP] Silva, Jeferson Junior da Cruz, Fernando Moraes Martins, Adriano Luis Hofling, Jose Francisco Palomari Spolidorio, Denise Madalena [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Estadual de Campinas (UNICAMP) Universidade Federal de São Carlos (UFSCar) |
dc.contributor.author.fl_str_mv |
Carvalho Bernardo, Wagner Luis de [UNESP] Gomes Boriollo, Marcelo Fabiano Tonon, Caroline Coradi [UNESP] Silva, Jeferson Junior da Cruz, Fernando Moraes Martins, Adriano Luis Hofling, Jose Francisco Palomari Spolidorio, Denise Madalena [UNESP] |
dc.subject.por.fl_str_mv |
Antimicrobial bioactivity GC-MS Green synthesis Medical and oral pathogens Plant extract |
topic |
Antimicrobial bioactivity GC-MS Green synthesis Medical and oral pathogens Plant extract |
description |
Objective: This study aimed to analyze the antimicrobial effects of lyophilized hydroalcoholic extract (HEScSeed and HEScFlower) and silver nanoparticles (AgNPs-HEScSeed and AgNPs-HEScFlower) of S. cumini seed and flower, and to characterize some compounds of these extracts and their NPs. Design: Phytochemical screening was performed by GC-MS. Nanoparticles were characterized by UV-vis spectroscopy, energy-dispersive X-ray (EDX) spectrophotometry, scanning electron microscopy (SEM) and field emission gun (FEG), dynamic light scattering (DLS) and zeta potential (ZP). Antimicrobial susceptibility tests were analyzed by broth microdilution and agar diffusion methods. Results: HEScSeed and HEScFlower showed 7 and 17 phytochemical compounds, respectively. AgNPs-plant extracts were reported as stable and with variable shapes and sizes. All studied species (A. naeslundii, C. albicans, F. nucleatum, S. aureus, S. epidermidis, S. mutans, S. oralis and V. dispar) were susceptible to extracts and AgNPs-plant extracts, with varying degrees of antimicrobial activities (extract: 648.4-5,187.5 mu g/mL; AgNPs-plant: 31.2-2,000 mu g/mL). Conclusion: The extracts of S. cumini seed and flower have antimicrobial action against pathogens of medical and dental interest, whose MIC and MMC are species-dependent. The AgNPs-HEScSeed and AgNPs-HEScFlower have different shapes, sizes, organic compounds, stability and electronegativity (capping), characteristics that contribute to their bacteriostatic and fungistatic effects, but at significantly lower concentrations than plant extracts. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T15:02:13Z 2021-06-25T15:02:13Z 2021-05-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.archoralbio.2021.105101 Archives Of Oral Biology. Oxford: Pergamon-elsevier Science Ltd, v. 125, 12 p., 2021. 0003-9969 http://hdl.handle.net/11449/210233 10.1016/j.archoralbio.2021.105101 WOS:000640056400012 |
url |
http://dx.doi.org/10.1016/j.archoralbio.2021.105101 http://hdl.handle.net/11449/210233 |
identifier_str_mv |
Archives Of Oral Biology. Oxford: Pergamon-elsevier Science Ltd, v. 125, 12 p., 2021. 0003-9969 10.1016/j.archoralbio.2021.105101 WOS:000640056400012 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Archives Of Oral Biology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
12 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129314263138304 |