Photoprotection and antioxidative metabolism in Ulva lactuca exposed to coastal oceanic acidification scenarios in the presence of Irgarol

Detalhes bibliográficos
Autor(a) principal: Sousa, Gabriela Tavares [UNESP]
Data de Publicação: 2021
Outros Autores: Lima Neto, Milton C. [UNESP], Choueri, Rodrigo Brasil, Castro, Italo Braga
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.aquatox.2020.105717
http://hdl.handle.net/11449/209811
Resumo: Anthropogenic changes such as ocean acidification, eutrophication, and the release of hazardous chemicals affect coastal environments and aquatic organisms. We investigated the effects of seawater pH (7.4 and 8.2) isolated and in combination with Irgarol on Ulva lactuca. Stress indicators such as membrane damage, lipid peroxidation, and hydrogen peroxide content were assessed. In addition, chlorophyll fluorescence and antioxidant enzyme activities were measured. The photosynthetic yield was affected by low pH in assays with and without Irgarol. However, the combination of low pH and Irgarol promoted photoinhibition, besides the induction of nonphotochemical quenching (NPQ) and changes in photosynthetic pigment contents. The induction of NPQ was directly influenced by low pH. The membrane damage was increased in low pH with and without Irgarol exposure. Total soluble protein and carbohydrate contents decreased in low pH, and in presence of Irgarol. The H2O2 content and lipid pemxidation were not affected by low pH. In contrast, Irgaml exposure strongly increased lipid peroxidation in both pHs, suggesting a possible synergistic effect. To avoid the harmful effects of high H2O2, U. lactuca increased antioxidant enzyme activities in treatments under low pH and in presence of Irgaml. Our results indicate that U. lactuca is tolerant to low pH by inducing NPQ, changing pigment contents, and increasing antioxidant defenses. In contrast, these protective mechanisms could not avoid the harmful effects of the combination with Irgaml.
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spelling Photoprotection and antioxidative metabolism in Ulva lactuca exposed to coastal oceanic acidification scenarios in the presence of IrgarolAntioxidant metabolismPhotosynthesisTriazineNPQOcean acidificationAnthropogenic changes such as ocean acidification, eutrophication, and the release of hazardous chemicals affect coastal environments and aquatic organisms. We investigated the effects of seawater pH (7.4 and 8.2) isolated and in combination with Irgarol on Ulva lactuca. Stress indicators such as membrane damage, lipid peroxidation, and hydrogen peroxide content were assessed. In addition, chlorophyll fluorescence and antioxidant enzyme activities were measured. The photosynthetic yield was affected by low pH in assays with and without Irgarol. However, the combination of low pH and Irgarol promoted photoinhibition, besides the induction of nonphotochemical quenching (NPQ) and changes in photosynthetic pigment contents. The induction of NPQ was directly influenced by low pH. The membrane damage was increased in low pH with and without Irgarol exposure. Total soluble protein and carbohydrate contents decreased in low pH, and in presence of Irgarol. The H2O2 content and lipid pemxidation were not affected by low pH. In contrast, Irgaml exposure strongly increased lipid peroxidation in both pHs, suggesting a possible synergistic effect. To avoid the harmful effects of high H2O2, U. lactuca increased antioxidant enzyme activities in treatments under low pH and in presence of Irgaml. Our results indicate that U. lactuca is tolerant to low pH by inducing NPQ, changing pigment contents, and increasing antioxidant defenses. In contrast, these protective mechanisms could not avoid the harmful effects of the combination with Irgaml.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Fed Sao Paulo, Inst Mar, Rua Maria Maximo 168, BR-11030400 Santos, SP, BrazilState Univ Sao Paulo, Biosci Inst, Coastal Campus,Praca Infante Dom Henrique S-N, BR-11330900 Sao Vicente, SP, BrazilState Univ Sao Paulo, Biosci Inst, Coastal Campus,Praca Infante Dom Henrique S-N, BR-11330900 Sao Vicente, SP, BrazilFAPESP: 2018/0458-6CNPq: 423583/2018-2CAPES: 001CNPq: PQ 302713/2018-2CNPq: PQ 301766/2019-3Elsevier B.V.Universidade Federal de São Paulo (UNIFESP)Universidade Estadual Paulista (Unesp)Sousa, Gabriela Tavares [UNESP]Lima Neto, Milton C. [UNESP]Choueri, Rodrigo BrasilCastro, Italo Braga2021-06-25T12:30:03Z2021-06-25T12:30:03Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9http://dx.doi.org/10.1016/j.aquatox.2020.105717Aquatic Toxicology. Amsterdam: Elsevier, v. 230, 9 p., 2021.0166-445Xhttp://hdl.handle.net/11449/20981110.1016/j.aquatox.2020.105717WOS:000600837200025Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAquatic Toxicologyinfo:eu-repo/semantics/openAccess2021-10-23T19:50:02Zoai:repositorio.unesp.br:11449/209811Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T19:50:02Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Photoprotection and antioxidative metabolism in Ulva lactuca exposed to coastal oceanic acidification scenarios in the presence of Irgarol
title Photoprotection and antioxidative metabolism in Ulva lactuca exposed to coastal oceanic acidification scenarios in the presence of Irgarol
spellingShingle Photoprotection and antioxidative metabolism in Ulva lactuca exposed to coastal oceanic acidification scenarios in the presence of Irgarol
Sousa, Gabriela Tavares [UNESP]
Antioxidant metabolism
Photosynthesis
Triazine
NPQ
Ocean acidification
title_short Photoprotection and antioxidative metabolism in Ulva lactuca exposed to coastal oceanic acidification scenarios in the presence of Irgarol
title_full Photoprotection and antioxidative metabolism in Ulva lactuca exposed to coastal oceanic acidification scenarios in the presence of Irgarol
title_fullStr Photoprotection and antioxidative metabolism in Ulva lactuca exposed to coastal oceanic acidification scenarios in the presence of Irgarol
title_full_unstemmed Photoprotection and antioxidative metabolism in Ulva lactuca exposed to coastal oceanic acidification scenarios in the presence of Irgarol
title_sort Photoprotection and antioxidative metabolism in Ulva lactuca exposed to coastal oceanic acidification scenarios in the presence of Irgarol
author Sousa, Gabriela Tavares [UNESP]
author_facet Sousa, Gabriela Tavares [UNESP]
Lima Neto, Milton C. [UNESP]
Choueri, Rodrigo Brasil
Castro, Italo Braga
author_role author
author2 Lima Neto, Milton C. [UNESP]
Choueri, Rodrigo Brasil
Castro, Italo Braga
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Sousa, Gabriela Tavares [UNESP]
Lima Neto, Milton C. [UNESP]
Choueri, Rodrigo Brasil
Castro, Italo Braga
dc.subject.por.fl_str_mv Antioxidant metabolism
Photosynthesis
Triazine
NPQ
Ocean acidification
topic Antioxidant metabolism
Photosynthesis
Triazine
NPQ
Ocean acidification
description Anthropogenic changes such as ocean acidification, eutrophication, and the release of hazardous chemicals affect coastal environments and aquatic organisms. We investigated the effects of seawater pH (7.4 and 8.2) isolated and in combination with Irgarol on Ulva lactuca. Stress indicators such as membrane damage, lipid peroxidation, and hydrogen peroxide content were assessed. In addition, chlorophyll fluorescence and antioxidant enzyme activities were measured. The photosynthetic yield was affected by low pH in assays with and without Irgarol. However, the combination of low pH and Irgarol promoted photoinhibition, besides the induction of nonphotochemical quenching (NPQ) and changes in photosynthetic pigment contents. The induction of NPQ was directly influenced by low pH. The membrane damage was increased in low pH with and without Irgarol exposure. Total soluble protein and carbohydrate contents decreased in low pH, and in presence of Irgarol. The H2O2 content and lipid pemxidation were not affected by low pH. In contrast, Irgaml exposure strongly increased lipid peroxidation in both pHs, suggesting a possible synergistic effect. To avoid the harmful effects of high H2O2, U. lactuca increased antioxidant enzyme activities in treatments under low pH and in presence of Irgaml. Our results indicate that U. lactuca is tolerant to low pH by inducing NPQ, changing pigment contents, and increasing antioxidant defenses. In contrast, these protective mechanisms could not avoid the harmful effects of the combination with Irgaml.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T12:30:03Z
2021-06-25T12:30:03Z
2021-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.aquatox.2020.105717
Aquatic Toxicology. Amsterdam: Elsevier, v. 230, 9 p., 2021.
0166-445X
http://hdl.handle.net/11449/209811
10.1016/j.aquatox.2020.105717
WOS:000600837200025
url http://dx.doi.org/10.1016/j.aquatox.2020.105717
http://hdl.handle.net/11449/209811
identifier_str_mv Aquatic Toxicology. Amsterdam: Elsevier, v. 230, 9 p., 2021.
0166-445X
10.1016/j.aquatox.2020.105717
WOS:000600837200025
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Aquatic Toxicology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 9
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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