Photoprotection and antioxidative metabolism in Ulva lactuca exposed to coastal oceanic acidification scenarios in the presence of Irgarol
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.aquatox.2020.105717 http://hdl.handle.net/11449/209811 |
Resumo: | Anthropogenic changes such as ocean acidification, eutrophication, and the release of hazardous chemicals affect coastal environments and aquatic organisms. We investigated the effects of seawater pH (7.4 and 8.2) isolated and in combination with Irgarol on Ulva lactuca. Stress indicators such as membrane damage, lipid peroxidation, and hydrogen peroxide content were assessed. In addition, chlorophyll fluorescence and antioxidant enzyme activities were measured. The photosynthetic yield was affected by low pH in assays with and without Irgarol. However, the combination of low pH and Irgarol promoted photoinhibition, besides the induction of nonphotochemical quenching (NPQ) and changes in photosynthetic pigment contents. The induction of NPQ was directly influenced by low pH. The membrane damage was increased in low pH with and without Irgarol exposure. Total soluble protein and carbohydrate contents decreased in low pH, and in presence of Irgarol. The H2O2 content and lipid pemxidation were not affected by low pH. In contrast, Irgaml exposure strongly increased lipid peroxidation in both pHs, suggesting a possible synergistic effect. To avoid the harmful effects of high H2O2, U. lactuca increased antioxidant enzyme activities in treatments under low pH and in presence of Irgaml. Our results indicate that U. lactuca is tolerant to low pH by inducing NPQ, changing pigment contents, and increasing antioxidant defenses. In contrast, these protective mechanisms could not avoid the harmful effects of the combination with Irgaml. |
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Photoprotection and antioxidative metabolism in Ulva lactuca exposed to coastal oceanic acidification scenarios in the presence of IrgarolAntioxidant metabolismPhotosynthesisTriazineNPQOcean acidificationAnthropogenic changes such as ocean acidification, eutrophication, and the release of hazardous chemicals affect coastal environments and aquatic organisms. We investigated the effects of seawater pH (7.4 and 8.2) isolated and in combination with Irgarol on Ulva lactuca. Stress indicators such as membrane damage, lipid peroxidation, and hydrogen peroxide content were assessed. In addition, chlorophyll fluorescence and antioxidant enzyme activities were measured. The photosynthetic yield was affected by low pH in assays with and without Irgarol. However, the combination of low pH and Irgarol promoted photoinhibition, besides the induction of nonphotochemical quenching (NPQ) and changes in photosynthetic pigment contents. The induction of NPQ was directly influenced by low pH. The membrane damage was increased in low pH with and without Irgarol exposure. Total soluble protein and carbohydrate contents decreased in low pH, and in presence of Irgarol. The H2O2 content and lipid pemxidation were not affected by low pH. In contrast, Irgaml exposure strongly increased lipid peroxidation in both pHs, suggesting a possible synergistic effect. To avoid the harmful effects of high H2O2, U. lactuca increased antioxidant enzyme activities in treatments under low pH and in presence of Irgaml. Our results indicate that U. lactuca is tolerant to low pH by inducing NPQ, changing pigment contents, and increasing antioxidant defenses. In contrast, these protective mechanisms could not avoid the harmful effects of the combination with Irgaml.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Fed Sao Paulo, Inst Mar, Rua Maria Maximo 168, BR-11030400 Santos, SP, BrazilState Univ Sao Paulo, Biosci Inst, Coastal Campus,Praca Infante Dom Henrique S-N, BR-11330900 Sao Vicente, SP, BrazilState Univ Sao Paulo, Biosci Inst, Coastal Campus,Praca Infante Dom Henrique S-N, BR-11330900 Sao Vicente, SP, BrazilFAPESP: 2018/0458-6CNPq: 423583/2018-2CAPES: 001CNPq: PQ 302713/2018-2CNPq: PQ 301766/2019-3Elsevier B.V.Universidade Federal de São Paulo (UNIFESP)Universidade Estadual Paulista (Unesp)Sousa, Gabriela Tavares [UNESP]Lima Neto, Milton C. [UNESP]Choueri, Rodrigo BrasilCastro, Italo Braga2021-06-25T12:30:03Z2021-06-25T12:30:03Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9http://dx.doi.org/10.1016/j.aquatox.2020.105717Aquatic Toxicology. Amsterdam: Elsevier, v. 230, 9 p., 2021.0166-445Xhttp://hdl.handle.net/11449/20981110.1016/j.aquatox.2020.105717WOS:000600837200025Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAquatic Toxicologyinfo:eu-repo/semantics/openAccess2021-10-23T19:50:02Zoai:repositorio.unesp.br:11449/209811Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T19:50:02Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Photoprotection and antioxidative metabolism in Ulva lactuca exposed to coastal oceanic acidification scenarios in the presence of Irgarol |
title |
Photoprotection and antioxidative metabolism in Ulva lactuca exposed to coastal oceanic acidification scenarios in the presence of Irgarol |
spellingShingle |
Photoprotection and antioxidative metabolism in Ulva lactuca exposed to coastal oceanic acidification scenarios in the presence of Irgarol Sousa, Gabriela Tavares [UNESP] Antioxidant metabolism Photosynthesis Triazine NPQ Ocean acidification |
title_short |
Photoprotection and antioxidative metabolism in Ulva lactuca exposed to coastal oceanic acidification scenarios in the presence of Irgarol |
title_full |
Photoprotection and antioxidative metabolism in Ulva lactuca exposed to coastal oceanic acidification scenarios in the presence of Irgarol |
title_fullStr |
Photoprotection and antioxidative metabolism in Ulva lactuca exposed to coastal oceanic acidification scenarios in the presence of Irgarol |
title_full_unstemmed |
Photoprotection and antioxidative metabolism in Ulva lactuca exposed to coastal oceanic acidification scenarios in the presence of Irgarol |
title_sort |
Photoprotection and antioxidative metabolism in Ulva lactuca exposed to coastal oceanic acidification scenarios in the presence of Irgarol |
author |
Sousa, Gabriela Tavares [UNESP] |
author_facet |
Sousa, Gabriela Tavares [UNESP] Lima Neto, Milton C. [UNESP] Choueri, Rodrigo Brasil Castro, Italo Braga |
author_role |
author |
author2 |
Lima Neto, Milton C. [UNESP] Choueri, Rodrigo Brasil Castro, Italo Braga |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Sousa, Gabriela Tavares [UNESP] Lima Neto, Milton C. [UNESP] Choueri, Rodrigo Brasil Castro, Italo Braga |
dc.subject.por.fl_str_mv |
Antioxidant metabolism Photosynthesis Triazine NPQ Ocean acidification |
topic |
Antioxidant metabolism Photosynthesis Triazine NPQ Ocean acidification |
description |
Anthropogenic changes such as ocean acidification, eutrophication, and the release of hazardous chemicals affect coastal environments and aquatic organisms. We investigated the effects of seawater pH (7.4 and 8.2) isolated and in combination with Irgarol on Ulva lactuca. Stress indicators such as membrane damage, lipid peroxidation, and hydrogen peroxide content were assessed. In addition, chlorophyll fluorescence and antioxidant enzyme activities were measured. The photosynthetic yield was affected by low pH in assays with and without Irgarol. However, the combination of low pH and Irgarol promoted photoinhibition, besides the induction of nonphotochemical quenching (NPQ) and changes in photosynthetic pigment contents. The induction of NPQ was directly influenced by low pH. The membrane damage was increased in low pH with and without Irgarol exposure. Total soluble protein and carbohydrate contents decreased in low pH, and in presence of Irgarol. The H2O2 content and lipid pemxidation were not affected by low pH. In contrast, Irgaml exposure strongly increased lipid peroxidation in both pHs, suggesting a possible synergistic effect. To avoid the harmful effects of high H2O2, U. lactuca increased antioxidant enzyme activities in treatments under low pH and in presence of Irgaml. Our results indicate that U. lactuca is tolerant to low pH by inducing NPQ, changing pigment contents, and increasing antioxidant defenses. In contrast, these protective mechanisms could not avoid the harmful effects of the combination with Irgaml. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T12:30:03Z 2021-06-25T12:30:03Z 2021-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.aquatox.2020.105717 Aquatic Toxicology. Amsterdam: Elsevier, v. 230, 9 p., 2021. 0166-445X http://hdl.handle.net/11449/209811 10.1016/j.aquatox.2020.105717 WOS:000600837200025 |
url |
http://dx.doi.org/10.1016/j.aquatox.2020.105717 http://hdl.handle.net/11449/209811 |
identifier_str_mv |
Aquatic Toxicology. Amsterdam: Elsevier, v. 230, 9 p., 2021. 0166-445X 10.1016/j.aquatox.2020.105717 WOS:000600837200025 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Aquatic Toxicology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
9 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965716253769728 |