Single intraoperative infrared laser optimized bone repair in rat femoral osteotomies with experimentally induced osteoporosis
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10103-023-03746-w http://hdl.handle.net/11449/249782 |
Resumo: | This study aimed to evaluate the effect of infrared laser (IRL) on bone repair in ovariectomized rats subjected to femoral osteotomies. Of 32 rats, half underwent bilateral ovariectomy (OVX) and the other half underwent sham ovariectomy (SHAM). A period of 3 months was defined to observe the presence of osteoporosis. The rats were subjected to osteotomies in the femurs and then fixed with a miniplate and 1.5-mm system screws. Thereafter, half of the rats from both SHAM and OVX groups were not irradiated, and the other half were irradiated by IRL using the following parameters: wavelength, 808 nm; power, 100 mW; 60 s for each point; 6 J/point; and a total of 5 points of bone gap. All animals were euthanized 60 days after surgery. The femur gap was scanned using micro-computed tomography (micro-CT). The samples were then examined under a confocal laser microscope to determine the amounts of calcein and alizarin red. The slides were stained with alizarin red and Stevenel’s blue for histometric analysis. In the micro-CT analysis, the OVX groups had the lowest bone volume (P < 0.05). When the laser was applied to the OVX groups, bone turnover increased (P < 0.05). New bone formation (NBF) was comparable between SHAM and OVX/IR (P > 0.05) groups; however, it was less in the OVX groups (P < 0.05). In conclusion, the results encourage the use of IRL intraoperatively as it optimizes bone repair, mainly in animals with low bone mineral density. |
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Repositório Institucional da UNESP |
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Single intraoperative infrared laser optimized bone repair in rat femoral osteotomies with experimentally induced osteoporosisBone regenerationFracture fixation internalLaser therapyOsteoporosisThis study aimed to evaluate the effect of infrared laser (IRL) on bone repair in ovariectomized rats subjected to femoral osteotomies. Of 32 rats, half underwent bilateral ovariectomy (OVX) and the other half underwent sham ovariectomy (SHAM). A period of 3 months was defined to observe the presence of osteoporosis. The rats were subjected to osteotomies in the femurs and then fixed with a miniplate and 1.5-mm system screws. Thereafter, half of the rats from both SHAM and OVX groups were not irradiated, and the other half were irradiated by IRL using the following parameters: wavelength, 808 nm; power, 100 mW; 60 s for each point; 6 J/point; and a total of 5 points of bone gap. All animals were euthanized 60 days after surgery. The femur gap was scanned using micro-computed tomography (micro-CT). The samples were then examined under a confocal laser microscope to determine the amounts of calcein and alizarin red. The slides were stained with alizarin red and Stevenel’s blue for histometric analysis. In the micro-CT analysis, the OVX groups had the lowest bone volume (P < 0.05). When the laser was applied to the OVX groups, bone turnover increased (P < 0.05). New bone formation (NBF) was comparable between SHAM and OVX/IR (P > 0.05) groups; however, it was less in the OVX groups (P < 0.05). In conclusion, the results encourage the use of IRL intraoperatively as it optimizes bone repair, mainly in animals with low bone mineral density.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)School of Dentistry São Paulo State University (UNESP), São PauloDepartment of Implantology University of Santo Amaro, Unisa, SPDiagnosis and Surgery Department School of Dentistry São Paulo State University (UNESP), 1193 José Bonifácio Street, São PauloSchool of Dentistry São Paulo State University (UNESP), São PauloDiagnosis and Surgery Department School of Dentistry São Paulo State University (UNESP), 1193 José Bonifácio Street, São PauloFAPESP: 2016/20297-6FAPESP: 2018/11496-0Universidade Estadual Paulista (UNESP)University of Santo AmaroPolo, Tárik Ocon Braga [UNESP]Fonseca-Santos, João Matheus [UNESP]Momesso, Gustavo Antonio Correa [UNESP]da Silva, William Phillip Pereira [UNESP]Barbosa, Stefany [UNESP]Santos, Anderson Maikon de Souza [UNESP]Silva, Mirela Caroline [UNESP]Garcia, Valdir Gouveia [UNESP]Theodoro, Letícia Helena [UNESP]Faverani, Leonardo P. [UNESP]2023-07-29T16:09:03Z2023-07-29T16:09:03Z2023-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s10103-023-03746-wLasers in Medical Science, v. 38, n. 1, 2023.1435-604X0268-8921http://hdl.handle.net/11449/24978210.1007/s10103-023-03746-w2-s2.0-85150531570Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLasers in Medical Scienceinfo:eu-repo/semantics/openAccess2023-07-29T16:09:03Zoai:repositorio.unesp.br:11449/249782Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:35:32.197672Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Single intraoperative infrared laser optimized bone repair in rat femoral osteotomies with experimentally induced osteoporosis |
title |
Single intraoperative infrared laser optimized bone repair in rat femoral osteotomies with experimentally induced osteoporosis |
spellingShingle |
Single intraoperative infrared laser optimized bone repair in rat femoral osteotomies with experimentally induced osteoporosis Polo, Tárik Ocon Braga [UNESP] Bone regeneration Fracture fixation internal Laser therapy Osteoporosis |
title_short |
Single intraoperative infrared laser optimized bone repair in rat femoral osteotomies with experimentally induced osteoporosis |
title_full |
Single intraoperative infrared laser optimized bone repair in rat femoral osteotomies with experimentally induced osteoporosis |
title_fullStr |
Single intraoperative infrared laser optimized bone repair in rat femoral osteotomies with experimentally induced osteoporosis |
title_full_unstemmed |
Single intraoperative infrared laser optimized bone repair in rat femoral osteotomies with experimentally induced osteoporosis |
title_sort |
Single intraoperative infrared laser optimized bone repair in rat femoral osteotomies with experimentally induced osteoporosis |
author |
Polo, Tárik Ocon Braga [UNESP] |
author_facet |
Polo, Tárik Ocon Braga [UNESP] Fonseca-Santos, João Matheus [UNESP] Momesso, Gustavo Antonio Correa [UNESP] da Silva, William Phillip Pereira [UNESP] Barbosa, Stefany [UNESP] Santos, Anderson Maikon de Souza [UNESP] Silva, Mirela Caroline [UNESP] Garcia, Valdir Gouveia [UNESP] Theodoro, Letícia Helena [UNESP] Faverani, Leonardo P. [UNESP] |
author_role |
author |
author2 |
Fonseca-Santos, João Matheus [UNESP] Momesso, Gustavo Antonio Correa [UNESP] da Silva, William Phillip Pereira [UNESP] Barbosa, Stefany [UNESP] Santos, Anderson Maikon de Souza [UNESP] Silva, Mirela Caroline [UNESP] Garcia, Valdir Gouveia [UNESP] Theodoro, Letícia Helena [UNESP] Faverani, Leonardo P. [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) University of Santo Amaro |
dc.contributor.author.fl_str_mv |
Polo, Tárik Ocon Braga [UNESP] Fonseca-Santos, João Matheus [UNESP] Momesso, Gustavo Antonio Correa [UNESP] da Silva, William Phillip Pereira [UNESP] Barbosa, Stefany [UNESP] Santos, Anderson Maikon de Souza [UNESP] Silva, Mirela Caroline [UNESP] Garcia, Valdir Gouveia [UNESP] Theodoro, Letícia Helena [UNESP] Faverani, Leonardo P. [UNESP] |
dc.subject.por.fl_str_mv |
Bone regeneration Fracture fixation internal Laser therapy Osteoporosis |
topic |
Bone regeneration Fracture fixation internal Laser therapy Osteoporosis |
description |
This study aimed to evaluate the effect of infrared laser (IRL) on bone repair in ovariectomized rats subjected to femoral osteotomies. Of 32 rats, half underwent bilateral ovariectomy (OVX) and the other half underwent sham ovariectomy (SHAM). A period of 3 months was defined to observe the presence of osteoporosis. The rats were subjected to osteotomies in the femurs and then fixed with a miniplate and 1.5-mm system screws. Thereafter, half of the rats from both SHAM and OVX groups were not irradiated, and the other half were irradiated by IRL using the following parameters: wavelength, 808 nm; power, 100 mW; 60 s for each point; 6 J/point; and a total of 5 points of bone gap. All animals were euthanized 60 days after surgery. The femur gap was scanned using micro-computed tomography (micro-CT). The samples were then examined under a confocal laser microscope to determine the amounts of calcein and alizarin red. The slides were stained with alizarin red and Stevenel’s blue for histometric analysis. In the micro-CT analysis, the OVX groups had the lowest bone volume (P < 0.05). When the laser was applied to the OVX groups, bone turnover increased (P < 0.05). New bone formation (NBF) was comparable between SHAM and OVX/IR (P > 0.05) groups; however, it was less in the OVX groups (P < 0.05). In conclusion, the results encourage the use of IRL intraoperatively as it optimizes bone repair, mainly in animals with low bone mineral density. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T16:09:03Z 2023-07-29T16:09:03Z 2023-12-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10103-023-03746-w Lasers in Medical Science, v. 38, n. 1, 2023. 1435-604X 0268-8921 http://hdl.handle.net/11449/249782 10.1007/s10103-023-03746-w 2-s2.0-85150531570 |
url |
http://dx.doi.org/10.1007/s10103-023-03746-w http://hdl.handle.net/11449/249782 |
identifier_str_mv |
Lasers in Medical Science, v. 38, n. 1, 2023. 1435-604X 0268-8921 10.1007/s10103-023-03746-w 2-s2.0-85150531570 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Lasers in Medical Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128952366006272 |