Transcriptome analysis in Parhyale hawaiensis reveal sex-specific responses to AgNP and AgCl exposure
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.envpol.2020.113963 http://hdl.handle.net/11449/195337 |
Resumo: | Analysis of the transcriptome of organisms exposed to toxicants offers new insights for ecotoxicology, but further research is needed to enhance interpretation of results and effectively incorporate them into useful environmental risk assessments. Factors that must be clarified to improve use of transcriptomics include assessment of the effect of organism sex within the context of toxicant exposure. Amphipods are well recognized as model organisms for toxicity evaluation because of their sensitivity and amenability to laboratory conditions. To investigate whether response to metals in crustaceans differs according to sex we analyzed the amphipod Parhyale hawaiensis after exposure to AgCl and Ag nanoparticles (AgNP) via contaminated food. Gene specific analysis and whole genome transcriptional profile of male and female organisms were performed by both RT-qPCR and RNA-seq. We observed that expression of transcripts of genes glutathione transferase (GST) did not differ among AgCl and AgNP treatments. Significant differences between males and females were observed after exposure to AgCl and AgNP. Males presented twice the number of differentially expressed genes in comparison to females, and more differentially expressed were observed after exposure to AgNP than AgCl treatments in both sexes. The genes that had the greatest change in expression relative to control were those genes related to peptidase and catalytic activity and chitin and carbohydrate metabolic processes. Our study is the first to demonstrate sex specific differences in the transcriptomes of amphipods upon exposure to toxicants and emphasizes the importance of considering gender in ecotoxicology. (C) 2020 Elsevier Ltd. All rights reserved. |
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Transcriptome analysis in Parhyale hawaiensis reveal sex-specific responses to AgNP and AgCl exposureAmphipodGene expressionGlutathione-S-TransferaseRNA-SeqRT-qPCRAnalysis of the transcriptome of organisms exposed to toxicants offers new insights for ecotoxicology, but further research is needed to enhance interpretation of results and effectively incorporate them into useful environmental risk assessments. Factors that must be clarified to improve use of transcriptomics include assessment of the effect of organism sex within the context of toxicant exposure. Amphipods are well recognized as model organisms for toxicity evaluation because of their sensitivity and amenability to laboratory conditions. To investigate whether response to metals in crustaceans differs according to sex we analyzed the amphipod Parhyale hawaiensis after exposure to AgCl and Ag nanoparticles (AgNP) via contaminated food. Gene specific analysis and whole genome transcriptional profile of male and female organisms were performed by both RT-qPCR and RNA-seq. We observed that expression of transcripts of genes glutathione transferase (GST) did not differ among AgCl and AgNP treatments. Significant differences between males and females were observed after exposure to AgCl and AgNP. Males presented twice the number of differentially expressed genes in comparison to females, and more differentially expressed were observed after exposure to AgNP than AgCl treatments in both sexes. The genes that had the greatest change in expression relative to control were those genes related to peptidase and catalytic activity and chitin and carbohydrate metabolic processes. Our study is the first to demonstrate sex specific differences in the transcriptomes of amphipods upon exposure to toxicants and emphasizes the importance of considering gender in ecotoxicology. (C) 2020 Elsevier Ltd. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Sao Paulo, Sch Pharmaceut Sci, BR-05508000 Sao Paulo, BrazilUniv Estadual Campinas, Sch Technol, BR-13484332 Limeira, SP, BrazilUniv Estadual Campinas, Sch Appl Sci, Lab Biotechnol, BR-13484350 Limeira, SP, BrazilSao Paulo State Univ, Inst Biosci, BR-13506900 Rio Claro, SP, BrazilUniv Estadual Campinas, Life Sci Core Facil LaCTAD, BR-13083886 Campinas, SP, BrazilHeriot Watt Univ, Sch Energy Geosci Infrastruct & Soc, Inst Life & Earth Sci, Edinburgh EH14 4AS, Midlothian, ScotlandUniv Tennessee, Ctr Environm Biotechnol, 676 Dabney Hall,1416 Circle Dr, Knoxville, TN 37996 USAUniv Estadual Campinas, Dept Biochem & Tissue Biol, BR-13083970 Campinas, SP, BrazilSao Paulo State Univ, Inst Biosci, BR-13506900 Rio Claro, SP, BrazilFAPESP: 2014/08829-7CNPq: 400362/2014-7CAPES: 001Elsevier B.V.Universidade de São Paulo (USP)Universidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (Unesp)Heriot Watt UnivUniv TennesseeArtal, Mariana ColettyPereira, Karina Danielle [UNESP]Luchessi, Augusto Ducati [UNESP]Okura, Vagner KatsumiHenry, Theodore BurdickMarques-Souza, HenriqueUmbuzeiro, Gisela de Aragao2020-12-10T17:31:06Z2020-12-10T17:31:06Z2020-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10http://dx.doi.org/10.1016/j.envpol.2020.113963Environmental Pollution. Oxford: Elsevier Sci Ltd, v. 260, 10 p., 2020.0269-7491http://hdl.handle.net/11449/19533710.1016/j.envpol.2020.113963WOS:000528537600137Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEnvironmental Pollutioninfo:eu-repo/semantics/openAccess2021-10-23T07:53:24Zoai:repositorio.unesp.br:11449/195337Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:50:42.610161Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Transcriptome analysis in Parhyale hawaiensis reveal sex-specific responses to AgNP and AgCl exposure |
title |
Transcriptome analysis in Parhyale hawaiensis reveal sex-specific responses to AgNP and AgCl exposure |
spellingShingle |
Transcriptome analysis in Parhyale hawaiensis reveal sex-specific responses to AgNP and AgCl exposure Artal, Mariana Coletty Amphipod Gene expression Glutathione-S-Transferase RNA-Seq RT-qPCR |
title_short |
Transcriptome analysis in Parhyale hawaiensis reveal sex-specific responses to AgNP and AgCl exposure |
title_full |
Transcriptome analysis in Parhyale hawaiensis reveal sex-specific responses to AgNP and AgCl exposure |
title_fullStr |
Transcriptome analysis in Parhyale hawaiensis reveal sex-specific responses to AgNP and AgCl exposure |
title_full_unstemmed |
Transcriptome analysis in Parhyale hawaiensis reveal sex-specific responses to AgNP and AgCl exposure |
title_sort |
Transcriptome analysis in Parhyale hawaiensis reveal sex-specific responses to AgNP and AgCl exposure |
author |
Artal, Mariana Coletty |
author_facet |
Artal, Mariana Coletty Pereira, Karina Danielle [UNESP] Luchessi, Augusto Ducati [UNESP] Okura, Vagner Katsumi Henry, Theodore Burdick Marques-Souza, Henrique Umbuzeiro, Gisela de Aragao |
author_role |
author |
author2 |
Pereira, Karina Danielle [UNESP] Luchessi, Augusto Ducati [UNESP] Okura, Vagner Katsumi Henry, Theodore Burdick Marques-Souza, Henrique Umbuzeiro, Gisela de Aragao |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual de Campinas (UNICAMP) Universidade Estadual Paulista (Unesp) Heriot Watt Univ Univ Tennessee |
dc.contributor.author.fl_str_mv |
Artal, Mariana Coletty Pereira, Karina Danielle [UNESP] Luchessi, Augusto Ducati [UNESP] Okura, Vagner Katsumi Henry, Theodore Burdick Marques-Souza, Henrique Umbuzeiro, Gisela de Aragao |
dc.subject.por.fl_str_mv |
Amphipod Gene expression Glutathione-S-Transferase RNA-Seq RT-qPCR |
topic |
Amphipod Gene expression Glutathione-S-Transferase RNA-Seq RT-qPCR |
description |
Analysis of the transcriptome of organisms exposed to toxicants offers new insights for ecotoxicology, but further research is needed to enhance interpretation of results and effectively incorporate them into useful environmental risk assessments. Factors that must be clarified to improve use of transcriptomics include assessment of the effect of organism sex within the context of toxicant exposure. Amphipods are well recognized as model organisms for toxicity evaluation because of their sensitivity and amenability to laboratory conditions. To investigate whether response to metals in crustaceans differs according to sex we analyzed the amphipod Parhyale hawaiensis after exposure to AgCl and Ag nanoparticles (AgNP) via contaminated food. Gene specific analysis and whole genome transcriptional profile of male and female organisms were performed by both RT-qPCR and RNA-seq. We observed that expression of transcripts of genes glutathione transferase (GST) did not differ among AgCl and AgNP treatments. Significant differences between males and females were observed after exposure to AgCl and AgNP. Males presented twice the number of differentially expressed genes in comparison to females, and more differentially expressed were observed after exposure to AgNP than AgCl treatments in both sexes. The genes that had the greatest change in expression relative to control were those genes related to peptidase and catalytic activity and chitin and carbohydrate metabolic processes. Our study is the first to demonstrate sex specific differences in the transcriptomes of amphipods upon exposure to toxicants and emphasizes the importance of considering gender in ecotoxicology. (C) 2020 Elsevier Ltd. All rights reserved. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-10T17:31:06Z 2020-12-10T17:31:06Z 2020-05-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.envpol.2020.113963 Environmental Pollution. Oxford: Elsevier Sci Ltd, v. 260, 10 p., 2020. 0269-7491 http://hdl.handle.net/11449/195337 10.1016/j.envpol.2020.113963 WOS:000528537600137 |
url |
http://dx.doi.org/10.1016/j.envpol.2020.113963 http://hdl.handle.net/11449/195337 |
identifier_str_mv |
Environmental Pollution. Oxford: Elsevier Sci Ltd, v. 260, 10 p., 2020. 0269-7491 10.1016/j.envpol.2020.113963 WOS:000528537600137 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Environmental Pollution |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
10 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129467814510592 |